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UniProtKB - Q9A9H3 (GDPPS_CAUVC)
Protein
GDP-perosamine synthase
Gene
per
Organism
Caulobacter vibrioides (strain ATCC 19089 / CB15) (Caulobacter crescentus)
Status
Functioni
Catalyzes the synthesis of GDP-perosamine from GDP-4-keto-6-deoxy-D-mannose and L-glutamate. Can use only L-glutamate as amino donor. In vitro, can also use GDP-4-keto-3,6-dideoxymannose to produce GDP-3-deoxyperosamine. Involved in the formation of S-LPS, which is required for attachment of the protein S-layer to the outer membrane surface.
3 PublicationsCatalytic activityi
- EC:2.6.1.1022 Publications
Cofactori
pyridoxal 5'-phosphate2 Publications
Kineticsi
kcat is 2.7 sec(-1) for GDP-4-keto-6-deoxy-D-mannose. kcat is 0.015 sec(-1) for GDP-4-keto-3,6-dideoxymannose.1 Publication
- KM=0.013 mM for GDP-4-keto-6-deoxy-D-mannose1 Publication
- KM=0.016 mM for GDP-4-keto-3,6-dideoxymannose1 Publication
- KM=4.6 mM for L-glutamate (with GDP-4-keto-6-deoxy-D-mannose as cosubstrate)1 Publication
- KM=0.13 mM for L-glutamate (with GDP-4-keto-3,6-dideoxymannose as cosubstrate)1 Publication
: LPS O-antigen biosynthesis Pathwayi
This protein is involved in the pathway LPS O-antigen biosynthesis, which is part of Bacterial outer membrane biogenesis.1 PublicationView all proteins of this organism that are known to be involved in the pathway LPS O-antigen biosynthesis and in Bacterial outer membrane biogenesis.
GO - Molecular functioni
- GDP-4-dehydro-6-deoxy-D-mannose-4-aminotransferase activity Source: UniProtKB-EC
GO - Biological processi
- O antigen biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Aminotransferase, Transferase |
Biological process | Lipopolysaccharide biosynthesis |
Ligand | Pyridoxal phosphate |
Enzyme and pathway databases
BioCyci | CAULO:CC1012-MONOMER |
BRENDAi | 2.6.1.102, 1218 |
UniPathwayi | UPA00281 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:per1 Publication Ordered Locus Names:CC_1012Imported |
Organismi | Caulobacter vibrioides (strain ATCC 19089 / CB15) (Caulobacter crescentus) |
Taxonomic identifieri | 190650 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Caulobacter › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Mutant produces altered S-LPS.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 186 | K → A: Loss of activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000430719 | 1 – 371 | GDP-perosamine synthaseAdd BLAST | 371 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 186 | N6-(pyridoxal phosphate)lysine1 PublicationBy similarity | 1 |
Interactioni
Subunit structurei
Homodimer.
1 PublicationProtein-protein interaction databases
STRINGi | 190650.CC_1012 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9A9H3 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9A9H3 |
Family & Domainsi
Sequence similaritiesi
Belongs to the DegT/DnrJ/EryC1 family.Curated
Phylogenomic databases
eggNOGi | COG0399, Bacteria |
HOGENOMi | CLU_033332_7_2_5 |
OMAi | GALVCHD |
Family and domain databases
CDDi | cd00616, AHBA_syn, 1 hit |
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR000653, DegT/StrS_aminotransferase IPR015424, PyrdxlP-dep_Trfase IPR015421, PyrdxlP-dep_Trfase_major IPR015422, PyrdxlP-dep_Trfase_small |
PANTHERi | PTHR30244, PTHR30244, 1 hit |
Pfami | View protein in Pfam PF01041, DegT_DnrJ_EryC1, 1 hit |
PIRSFi | PIRSF000390, PLP_StrS, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9A9H3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSDLPRISVA APRLDGNERD YVLECMDTTW ISSVGRFIVE FEKAFADYCG
60 70 80 90 100
VKHAIACNNG TTALHLALVA MGIGPGDEVI VPSLTYIASA NSVTYCGATP
110 120 130 140 150
VLVDNDPRTF NLDAAKLEAL ITPRTKAIMP VHLYGQICDM DPILEVARRH
160 170 180 190 200
NLLVIEDAAE AVGATYRGKK SGSLGDCATF SFFGNKIITT GEGGMITTND
210 220 230 240 250
DDLAAKMRLL RGQGMDPNRR YWFPIVGFNY RMTNIQAAIG LAQLERVDEH
260 270 280 290 300
LAARERVVGW YEQKLARLGN RVTKPHVALT GRHVFWMYTV RLGEGLSTTR
310 320 330 340 350
DQVIKDLDAL GIESRPVFHP MHIMPPYAHL ATDDLKIAEA CGVDGLNLPT
360 370
HAGLTEADID RVIAALDQVL V
Sequence cautioni
The sequence AAC38670 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF062345 Genomic DNA Translation: AAC38670.2 Different initiation. AE005673 Genomic DNA Translation: AAK22996.1 |
PIRi | H87374 |
RefSeqi | NP_419828.1, NC_002696.2 WP_010918896.1, NC_002696.2 |
Genome annotation databases
EnsemblBacteriai | AAK22996; AAK22996; CC_1012 |
KEGGi | ccr:CC_1012 |
PATRICi | fig|190650.5.peg.1030 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF062345 Genomic DNA Translation: AAC38670.2 Different initiation. AE005673 Genomic DNA Translation: AAK22996.1 |
PIRi | H87374 |
RefSeqi | NP_419828.1, NC_002696.2 WP_010918896.1, NC_002696.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3BN1 | X-ray | 1.80 | A/B/C/D | 2-371 | [»] | |
3DR4 | X-ray | 1.60 | A/B/C/D | 1-371 | [»] | |
3DR7 | X-ray | 1.70 | A/B/C/D | 1-371 | [»] | |
SMRi | Q9A9H3 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 190650.CC_1012 |
Genome annotation databases
EnsemblBacteriai | AAK22996; AAK22996; CC_1012 |
KEGGi | ccr:CC_1012 |
PATRICi | fig|190650.5.peg.1030 |
Phylogenomic databases
eggNOGi | COG0399, Bacteria |
HOGENOMi | CLU_033332_7_2_5 |
OMAi | GALVCHD |
Enzyme and pathway databases
UniPathwayi | UPA00281 |
BioCyci | CAULO:CC1012-MONOMER |
BRENDAi | 2.6.1.102, 1218 |
Miscellaneous databases
EvolutionaryTracei | Q9A9H3 |
Family and domain databases
CDDi | cd00616, AHBA_syn, 1 hit |
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR000653, DegT/StrS_aminotransferase IPR015424, PyrdxlP-dep_Trfase IPR015421, PyrdxlP-dep_Trfase_major IPR015422, PyrdxlP-dep_Trfase_small |
PANTHERi | PTHR30244, PTHR30244, 1 hit |
Pfami | View protein in Pfam PF01041, DegT_DnrJ_EryC1, 1 hit |
PIRSFi | PIRSF000390, PLP_StrS, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GDPPS_CAUVC | |
Accessioni | Q9A9H3Primary (citable) accession number: Q9A9H3 Secondary accession number(s): O85354 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 29, 2014 |
Last sequence update: | June 1, 2001 | |
Last modified: | September 29, 2021 | |
This is version 101 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families