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Protein

S-adenosylmethionine synthase

Gene

metK

Organism
Streptococcus pyogenes serotype M1
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme.UniRule annotation

Catalytic activityi

ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 2 divalent ions per subunit.UniRule annotation
  • K+UniRule annotationNote: Binds 1 potassium ion per subunit.UniRule annotation

Pathwayi: S-adenosyl-L-methionine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. S-adenosylmethionine synthase (metK)
This subpathway is part of the pathway S-adenosyl-L-methionine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine, the pathway S-adenosyl-L-methionine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei16ATPUniRule annotation1
Metal bindingi18MagnesiumUniRule annotation1
Metal bindingi44PotassiumUniRule annotation1
Binding sitei57MethionineUniRule annotation1
Binding sitei100MethionineUniRule annotation1
Binding sitei250ATP; shared with neighboring subunitUniRule annotation1
Binding sitei250Methionine; shared with neighboring subunitUniRule annotation1
Binding sitei273ATP; via amide nitrogen; shared with neighboring subunitUniRule annotation1
Binding sitei277ATP; shared with neighboring subunitUniRule annotation1
Binding sitei281MethionineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi174 – 176ATPUniRule annotation3
Nucleotide bindingi241 – 242ATPUniRule annotation2
Nucleotide bindingi256 – 257ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processOne-carbon metabolism
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium

Enzyme and pathway databases

UniPathwayiUPA00315; UER00080

Names & Taxonomyi

Protein namesi
Recommended name:
S-adenosylmethionine synthaseUniRule annotation (EC:2.5.1.6UniRule annotation)
Short name:
AdoMet synthaseUniRule annotation
Alternative name(s):
MATUniRule annotation
Methionine adenosyltransferaseUniRule annotation
Gene namesi
Name:metKUniRule annotation
Ordered Locus Names:SPy_1359, M5005_Spy1108
OrganismiStreptococcus pyogenes serotype M1
Taxonomic identifieri301447 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000000750 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001746031 – 398S-adenosylmethionine synthaseAdd BLAST398

Proteomic databases

PaxDbiQ99Z77
PRIDEiQ99Z77

Interactioni

Subunit structurei

Homotetramer; dimer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi160490.SPy_1359

Structurei

3D structure databases

ProteinModelPortaliQ99Z77
SMRiQ99Z77
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni100 – 110Flexible loopUniRule annotationAdd BLAST11

Sequence similaritiesi

Belongs to the AdoMet synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CPH Bacteria
COG0192 LUCA
HOGENOMiHOG000245710
KOiK00789
OMAiPGHFLFT

Family and domain databases

HAMAPiMF_00086 S_AdoMet_synth1, 1 hit
InterProiView protein in InterPro
IPR022631 ADOMET_SYNTHASE_CS
IPR022630 S-AdoMet_synt_C
IPR022629 S-AdoMet_synt_central
IPR022628 S-AdoMet_synt_N
IPR002133 S-AdoMet_synthetase
IPR022636 S-AdoMet_synthetase_sfam
PANTHERiPTHR11964 PTHR11964, 1 hit
PfamiView protein in Pfam
PF02773 S-AdoMet_synt_C, 1 hit
PF02772 S-AdoMet_synt_M, 1 hit
PF00438 S-AdoMet_synt_N, 1 hit
PIRSFiPIRSF000497 MAT, 1 hit
SUPFAMiSSF55973 SSF55973, 3 hits
TIGRFAMsiTIGR01034 metK, 1 hit
PROSITEiView protein in PROSITE
PS00376 ADOMET_SYNTHASE_1, 1 hit
PS00377 ADOMET_SYNTHASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q99Z77-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSERKLFTSE SVSEGHPDKI ADQISDAILD AILAEDPEAH VAAETCVYTG
60 70 80 90 100
SVHVFGEIST TAYIDINRVV RDTIAEIGYT EAEYGFSAES VGVHPSLVEQ
110 120 130 140 150
SGDIAQGVNE AFESREGDTD DLSHIGAGDQ GLMFGFAINE TPELMPLPIS
160 170 180 190 200
LSHQLVRRLA ELRKSGEISY LRPDAKSQVT VEYDEHDKPV RVDTVVISTQ
210 220 230 240 250
HDPEATNDQI RQDVIEKVIK AVIPADYLDD DTKFFINPTG RFVIGGPQGD
260 270 280 290 300
SGLTGRKIIV DTYGGYSRHG GGAFSGKDAT KVDRSASYAA RYIAKNLVAA
310 320 330 340 350
GLVTKAEVQL AYAIGVAQPV SVRVDTFGTS TVPEAVLEAA VRQVFDLRPA
360 370 380 390
GIIQMLDLKR PIYKQTAAYG HMGRTDIDLP WERLNKVDAL VEAVKTVL
Length:398
Mass (Da):43,179
Last modified:June 1, 2001 - v1
Checksum:iEB9CD98127BC959B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004092 Genomic DNA Translation: AAK34187.1
CP000017 Genomic DNA Translation: AAZ51726.1
RefSeqiNP_269466.1, NC_002737.2

Genome annotation databases

EnsemblBacteriaiAAK34187; AAK34187; SPy_1359
AAZ51726; AAZ51726; M5005_Spy1108
GeneIDi901434
KEGGispy:SPy_1359
spz:M5005_Spy1108
PATRICifig|160490.10.peg.1186

Similar proteinsi

Entry informationi

Entry nameiMETK_STRP1
AccessioniPrimary (citable) accession number: Q99Z77
Secondary accession number(s): Q48Y46
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: June 1, 2001
Last modified: July 18, 2018
This is version 110 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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