UniProtKB - Q99XG5 (GYRA_STAAN)
Protein
DNA gyrase subunit A
Gene
gyrA
Organism
Staphylococcus aureus (strain N315)
Status
Functioni
A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.UniRule annotation
Miscellaneous
Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.UniRule annotation
Catalytic activityi
- ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotation EC:5.6.2.2
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 123 | O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- DNA binding Source: UniProtKB-UniRule
- DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity Source: UniProtKB-UniRule
GO - Biological processi
- DNA-dependent DNA replication Source: UniProtKB-UniRule
- DNA topological change Source: UniProtKB-UniRule
- response to antibiotic Source: UniProtKB-KW
Keywordsi
Molecular function | DNA-binding, Isomerase, Topoisomerase |
Biological process | Antibiotic resistance |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | SAUR158879:G1G21-6-MONOMER |
BRENDAi | 5.99.1.3, 3352 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:gyrAUniRule annotation Ordered Locus Names:SA0006 |
Organismi | Staphylococcus aureus (strain N315) |
Taxonomic identifieri | 158879 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- chromosome Source: InterPro
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000145253 | 1 – 889 | DNA gyrase subunit AAdd BLAST | 889 |
Interactioni
Subunit structurei
Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.
UniRule annotationStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q99XG5 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q99XG5 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 528 – 534 | GyrA-boxUniRule annotation | 7 |
Sequence similaritiesi
Belongs to the type II topoisomerase GyrA/ParC subunit family.UniRule annotation
Phylogenomic databases
HOGENOMi | CLU_002977_6_1_9 |
OMAi | THHWLLF |
Family and domain databases
CDDi | cd00187, TOP4c, 1 hit |
Gene3Di | 1.10.268.10, 1 hit 2.120.10.90, 1 hit 3.90.199.10, 1 hit |
HAMAPi | MF_01897, GyrA, 1 hit |
InterProi | View protein in InterPro IPR005743, GyrA IPR006691, GyrA/parC_rep IPR035516, Gyrase/topoIV_suA_C IPR013760, Topo_IIA-like_dom_sf IPR002205, Topo_IIA_A/C IPR013758, Topo_IIA_A/C_ab IPR013757, Topo_IIA_A_a_sf |
Pfami | View protein in Pfam PF03989, DNA_gyraseA_C, 6 hits PF00521, DNA_topoisoIV, 1 hit |
SMARTi | View protein in SMART SM00434, TOP4c, 1 hit |
SUPFAMi | SSF101904, SSF101904, 1 hit SSF56719, SSF56719, 1 hit |
i Sequence
Sequence statusi: Complete.
Q99XG5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAELPQSRIN ERNITSEMRE SFLDYAMSVI VARALPDVRD GLKPVHRRIL
60 70 80 90 100
YGLNEQGMTP DKSYKKSARI VGDVMGKYHP HGDSSIYEAM VRMAQDFSYR
110 120 130 140 150
YPLVDGQGNF GSMDGDGAAA MRYTEARMTK ITLELLRDIN KDTIDFIDNY
160 170 180 190 200
DGNEREPSVL PARFPNLLAN GASGIAVGMA TNIPPHNLTE LINGVLSLSK
210 220 230 240 250
NPDISIAELM EDIEGPDFPT AGLILGKSGI RRAYETGRGS IQMRSRAVIE
260 270 280 290 300
ERGGGRQRIV VTEIPFQVNK ARMIEKIAEL VRDKKIDGIT DLRDETSLRT
310 320 330 340 350
GVRVVIDVRK DANASVILNN LYKQTPLQTS FGVNMIALVN GRPKLINLKE
360 370 380 390 400
ALVHYLEHQK TVVRRRTQYN LRKAKDRAHI LEGLRIALDH IDEIISTIRE
410 420 430 440 450
SDTDKVAMES LQQRFKLSEK QAQAILDMRL RRLTGLERDK IEAEYNELLN
460 470 480 490 500
YISELETILA DEEVLLQLVR DELTEIRDRF GDDRRTEIQL GGFEDLEDED
510 520 530 540 550
LIPEEQIVIT LSHNNYIKRL PVSTYRAQNR GGRGVQGMNT LEEDFVSQLV
560 570 580 590 600
TLSTHDHVLF FTNKGRVYKL KGYEVPELSR QSKGIPVVNA IELENDEVIS
610 620 630 640 650
TMIAVKDLES EDNFLVFATK RGVVKRSALS NFSRINRNGK IAISFREDDE
660 670 680 690 700
LIAVRLTSGQ EDILIGTSHA SLIRFPESTL RPLGRTATGV KGITLREGDE
710 720 730 740 750
VVGLDVAHAN SVDEVLVVTE NGYGKRTPVN DYRLSNRGGK GIKTATITER
760 770 780 790 800
NGNVVCITTV TGEEDLMIVT NAGVIIRLDV ADISQNGRAA QGVRLIRLGD
810 820 830 840 850
DQFVSTVAKV KEDAEDETNE DEQSTSTVSE DGTEQQREAV VNDETPGNAI
860 870 880
HTEVIDSEEN DEDGRIEVRQ DFMDRVEEDI QQSSDEDEE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BA000018 Genomic DNA Translation: BAB41222.1 |
PIRi | F89758 |
RefSeqi | WP_000819096.1, NC_002745.2 |
Genome annotation databases
EnsemblBacteriai | BAB41222; BAB41222; BAB41222 |
KEGGi | sau:SA0006 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BA000018 Genomic DNA Translation: BAB41222.1 |
PIRi | F89758 |
RefSeqi | WP_000819096.1, NC_002745.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2XCO | X-ray | 3.10 | A | 2-491 | [»] | |
2XCQ | X-ray | 2.98 | A | 2-491 | [»] | |
2XCR | X-ray | 3.50 | B/D/S/U | 2-491 | [»] | |
2XCS | X-ray | 2.10 | B/D | 2-491 | [»] | |
2XCT | X-ray | 3.35 | B/D/S/U | 2-491 | [»] | |
4BUL | X-ray | 2.60 | A/C | 2-491 | [»] | |
5CDM | X-ray | 2.50 | A/C | 9-490 | [»] | |
5CDN | X-ray | 2.79 | A/C/R/T | 10-490 | [»] | |
5CDO | X-ray | 3.15 | A/C/R/T | 9-490 | [»] | |
5CDP | X-ray | 2.45 | A/C | 9-491 | [»] | |
5CDQ | X-ray | 2.95 | A/C/R/T | 10-490 | [»] | |
5CDR | X-ray | 2.65 | A/C | 9-491 | [»] | |
5IWI | X-ray | 1.98 | A/C | 2-491 | [»] | |
5IWM | X-ray | 2.50 | A/C | 2-491 | [»] | |
5NPK | X-ray | 1.98 | B/D/b/d | 2-491 | [»] | |
5NPP | X-ray | 2.22 | B/D | 2-491 | [»] | |
6FM4 | X-ray | 2.70 | B/D | 2-491 | [»] | |
6FQM | X-ray | 3.06 | A/C/a/c | 2-491 | [»] | |
6FQS | X-ray | 3.11 | A/C | 2-491 | [»] | |
6FQV | X-ray | 2.60 | A/C/R/T | 2-491 | [»] | |
6QTK | X-ray | 2.31 | A/C | 2-491 | [»] | |
6QTP | X-ray | 2.37 | A/C | 2-491 | [»] | |
6QX1 | X-ray | 2.65 | A/C | 2-491 | [»] | |
SMRi | Q99XG5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Genome annotation databases
EnsemblBacteriai | BAB41222; BAB41222; BAB41222 |
KEGGi | sau:SA0006 |
Phylogenomic databases
HOGENOMi | CLU_002977_6_1_9 |
OMAi | THHWLLF |
Enzyme and pathway databases
BioCyci | SAUR158879:G1G21-6-MONOMER |
BRENDAi | 5.99.1.3, 3352 |
Miscellaneous databases
EvolutionaryTracei | Q99XG5 |
Family and domain databases
CDDi | cd00187, TOP4c, 1 hit |
Gene3Di | 1.10.268.10, 1 hit 2.120.10.90, 1 hit 3.90.199.10, 1 hit |
HAMAPi | MF_01897, GyrA, 1 hit |
InterProi | View protein in InterPro IPR005743, GyrA IPR006691, GyrA/parC_rep IPR035516, Gyrase/topoIV_suA_C IPR013760, Topo_IIA-like_dom_sf IPR002205, Topo_IIA_A/C IPR013758, Topo_IIA_A/C_ab IPR013757, Topo_IIA_A_a_sf |
Pfami | View protein in Pfam PF03989, DNA_gyraseA_C, 6 hits PF00521, DNA_topoisoIV, 1 hit |
SMARTi | View protein in SMART SM00434, TOP4c, 1 hit |
SUPFAMi | SSF101904, SSF101904, 1 hit SSF56719, SSF56719, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GYRA_STAAN | |
Accessioni | Q99XG5Primary (citable) accession number: Q99XG5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 28, 2003 |
Last sequence update: | June 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 126 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families