UniProtKB - Q99XG5 (GYRA_STAAN)

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History
Entry version 113 (23 May 2018)
Sequence version 1 (01 Jun 2001)
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Protein

DNA gyrase subunit A

Gene

gyrA

Organism

Staphylococcus aureus (strain N315)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.UniRule annotation

Miscellaneous

Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.UniRule annotation

Catalytic activityⁱ

ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotation

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Active siteⁱ	123	O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation		1

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-KW
DNA binding Source: UniProtKB-KW
DNA topoisomerase type II (ATP-hydrolyzing) activity Source: UniProtKB-EC

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

DNA topological change Source: InterPro
response to antibiotic Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	DNA-binding, Isomerase, Topoisomerase
Biological process	Antibiotic resistance
Ligand	ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAⁱ	5.99.1.3 3352

BRENDAⁱ

5.99.1.3 3352

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: DNA gyrase subunit AUniRule annotation (EC:5.99.1.3UniRule annotation)
Gene namesⁱ	Name:gyrAUniRule annotation Ordered Locus Names:SA0006
Organismⁱ	Staphylococcus aureus (strain N315)
Taxonomic identifierⁱ	158879 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Terrabacteria group › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › Staphylococcus aureus › Staphylococcus aureus subsp. aureus
Proteomesⁱ	UP000000751 Componentⁱ: Chromosome

Subcellular locationⁱ

Cytoplasm
UniRule annotation
Manual assertion according to rulesⁱ
- HAMAP-Rule:MF_01897

GO - Cellular componentⁱ

chromosome Source: InterPro
cytoplasm Source: UniProtKB-SubCell

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cytoplasm

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000145253	1 – 889	DNA gyrase subunit AAdd BLAST		889

Proteomic databases

PRIDEⁱ	Q99XG5

PRIDEⁱ

Q99XG5

Interactionⁱ

Subunit structureⁱ

Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.UniRule annotation

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Show more details

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	11 – 13	Combined sources	3
Helixⁱ	14 – 32	Combined sources	19
Turnⁱ	38 – 40	Combined sources	3
Helixⁱ	44 – 55	Combined sources	12
Beta strandⁱ	60 – 62	Combined sources	3
Helixⁱ	67 – 77	Combined sources	11
Helixⁱ	83 – 93	Combined sources	11
Turnⁱ	96 – 98	Combined sources	3
Beta strandⁱ	99 – 101	Combined sources	3
Beta strandⁱ	103 – 108	Combined sources	6
Turnⁱ	121 – 123	Combined sources	3
Beta strandⁱ	125 – 128	Combined sources	4
Helixⁱ	130 – 136	Combined sources	7
Turnⁱ	137 – 142	Combined sources	6
Beta strandⁱ	146 – 148	Combined sources	3
Beta strandⁱ	155 – 159	Combined sources	5
Helixⁱ	166 – 170	Combined sources	5
Beta strandⁱ	172 – 175	Combined sources	4
Beta strandⁱ	180 – 183	Combined sources	4
Helixⁱ	188 – 200	Combined sources	13
Helixⁱ	206 – 212	Combined sources	7
Beta strandⁱ	223 – 225	Combined sources	3
Helixⁱ	228 – 236	Combined sources	9
Beta strandⁱ	237 – 244	Combined sources	8
Beta strandⁱ	246 – 253	Combined sources	8
Beta strandⁱ	256 – 263	Combined sources	8
Helixⁱ	270 – 282	Combined sources	13
Beta strandⁱ	284 – 286	Combined sources	3
Beta strandⁱ	288 – 294	Combined sources	7
Turnⁱ	298 – 300	Combined sources	3
Beta strandⁱ	304 – 308	Combined sources	5
Beta strandⁱ	310 – 312	Combined sources	3
Helixⁱ	314 – 324	Combined sources	11
Beta strandⁱ	328 – 334	Combined sources	7
Beta strandⁱ	336 – 339	Combined sources	4
Beta strandⁱ	342 – 345	Combined sources	4
Helixⁱ	348 – 389	Combined sources	42
Helixⁱ	391 – 400	Combined sources	10
Helixⁱ	404 – 415	Combined sources	12
Helixⁱ	419 – 427	Combined sources	9
Helixⁱ	430 – 433	Combined sources	4
Beta strandⁱ	434 – 436	Combined sources	3
Helixⁱ	437 – 460	Combined sources	24
Helixⁱ	462 – 480	Combined sources	19
Beta strandⁱ	486 – 489	Combined sources	4

3D structure databases

ProteinModelPortalⁱ	Q99XG5
SMRⁱ	Q99XG5
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	Q99XG5

EvolutionaryTraceⁱ

Q99XG5

Family & Domainsⁱ

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	528 – 534	GyrA-boxUniRule annotation		7

Sequence similaritiesⁱ

Belongs to the type II topoisomerase GyrA/ParC subunit family.UniRule annotation

Phylogenomic databases

HOGENOMⁱ	HOG000076278
KEGG Orthology (KO) More...KOⁱ	K02469
OMAⁱ	THHWLLF

Family and domain databases

Gene3Dⁱ	1.10.268.10, 1 hit 2.120.10.90, 1 hit 3.90.199.10, 3 hits
HAMAPⁱ	MF_01897 GyrA, 1 hit
InterProⁱ	View protein in InterPro IPR005743 GyrA IPR006691 GyrA/parC_rep IPR035516 Gyrase/topoIV_suA_C IPR013760 Topo_IIA-like_dom_sf IPR002205 Topo_IIA_A/C IPR013758 Topo_IIA_A/C_ab IPR013757 Topo_IIA_A_a_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF03989 DNA_gyraseA_C, 6 hits PF00521 DNA_topoisoIV, 1 hit
SMARTⁱ	View protein in SMART SM00434 TOP4c, 1 hit
SUPFAMⁱ	SSF101904 SSF101904, 1 hit SSF56719 SSF56719, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q99XG5-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MAELPQSRIN ERNITSEMRE SFLDYAMSVI VARALPDVRD GLKPVHRRIL 
        60         70         80         90        100
YGLNEQGMTP DKSYKKSARI VGDVMGKYHP HGDSSIYEAM VRMAQDFSYR 
       110        120        130        140        150
YPLVDGQGNF GSMDGDGAAA MRYTEARMTK ITLELLRDIN KDTIDFIDNY 
       160        170        180        190        200
DGNEREPSVL PARFPNLLAN GASGIAVGMA TNIPPHNLTE LINGVLSLSK 
       210        220        230        240        250
NPDISIAELM EDIEGPDFPT AGLILGKSGI RRAYETGRGS IQMRSRAVIE 
       260        270        280        290        300
ERGGGRQRIV VTEIPFQVNK ARMIEKIAEL VRDKKIDGIT DLRDETSLRT 
       310        320        330        340        350
GVRVVIDVRK DANASVILNN LYKQTPLQTS FGVNMIALVN GRPKLINLKE 
       360        370        380        390        400
ALVHYLEHQK TVVRRRTQYN LRKAKDRAHI LEGLRIALDH IDEIISTIRE 
       410        420        430        440        450
SDTDKVAMES LQQRFKLSEK QAQAILDMRL RRLTGLERDK IEAEYNELLN 
       460        470        480        490        500
YISELETILA DEEVLLQLVR DELTEIRDRF GDDRRTEIQL GGFEDLEDED 
       510        520        530        540        550
LIPEEQIVIT LSHNNYIKRL PVSTYRAQNR GGRGVQGMNT LEEDFVSQLV 
       560        570        580        590        600
TLSTHDHVLF FTNKGRVYKL KGYEVPELSR QSKGIPVVNA IELENDEVIS 
       610        620        630        640        650
TMIAVKDLES EDNFLVFATK RGVVKRSALS NFSRINRNGK IAISFREDDE 
       660        670        680        690        700
LIAVRLTSGQ EDILIGTSHA SLIRFPESTL RPLGRTATGV KGITLREGDE 
       710        720        730        740        750
VVGLDVAHAN SVDEVLVVTE NGYGKRTPVN DYRLSNRGGK GIKTATITER 
       760        770        780        790        800
NGNVVCITTV TGEEDLMIVT NAGVIIRLDV ADISQNGRAA QGVRLIRLGD 
       810        820        830        840        850
DQFVSTVAKV KEDAEDETNE DEQSTSTVSE DGTEQQREAV VNDETPGNAI 
       860        870        880 
HTEVIDSEEN DEDGRIEVRQ DFMDRVEEDI QQSSDEDEE

Length:889

Mass (Da):99,625

Last modified:June 1, 2001 - v1

Checksum:ⁱDD5762C23EDC6E2F

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	BA000018 Genomic DNA Translation: BAB41222.1
PIRⁱ	F89758
NCBI Reference Sequences More...RefSeqⁱ	WP_000819096.1, NC_002745.2

Genome annotation databases

EnsemblBacteriaⁱ	BAB41222; BAB41222; BAB41222
KEGGⁱ	sau:SA0006

Protein	Similar proteins		Species	Score	Length
Q99XG5	DNA gyrase subunit A		STAA3		887
DNA gyrase subunit A		STAAU		887
DNA gyrase subunit A		Staphylococcus sp. HMSC055H04		887
DNA gyrase subunit A		Staphylococcus aureus subsp. aureus 71193		895
DNA gyrase subunit A		Staphylococcus aureus subsp. aureus CN1		895
+379

Protein	Similar proteins		Species	Score	Length
Q99XG5	DNA gyrase subunit A		STAA3		887
DNA gyrase subunit A		STAAU		887
DNA gyrase subunit A		Staphylococcus sp. HMSC055H04		887
DNA gyrase subunit A		Staphylococcus aureus subsp. aureus CN1		895
UPI0001AE9AFC		Staphylococcus		889
+1712

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2XCO	X-ray	3.10	A	2-491	[»]
2XCQ	X-ray	2.98	A	2-491	[»]
2XCR	X-ray	3.50	B/D/S/U	2-491	[»]
2XCS	X-ray	2.10	B/D	2-491	[»]
2XCT	X-ray	3.35	B/D/S/U	2-491	[»]
4BUL	X-ray	2.60	A/C	2-491	[»]
5CDM	X-ray	2.50	A/C	9-490	[»]
5CDN	X-ray	2.79	A/C/R/T	10-490	[»]
5CDO	X-ray	3.15	A/C/R/T	9-490	[»]
5CDP	X-ray	2.45	A/C	9-491	[»]
5CDQ	X-ray	2.95	A/C/R/T	10-490	[»]
5CDR	X-ray	2.65	A/C	9-491	[»]
5IWI	X-ray	1.98	A/C	2-491	[»]
5IWM	X-ray	2.50	A/C	2-491	[»]
5NPK	X-ray	1.98	B/D/b/d	2-491	[»]
5NPP	X-ray	2.22	B/D	2-491	[»]
6FQM	X-ray	3.06	A/C/a/c	2-491	[»]
6FQS	X-ray	3.11	A/C	2-491	[»]
6FQV	X-ray	2.60	A/C/R/T	2-491	[»]
ProteinModelPortalⁱ	Q99XG5
SMRⁱ	Q99XG5
ModBaseⁱ	Search...
MobiDBⁱ	Search...

PRIDEⁱ	Q99XG5

Structural Biology Knowledgebase	Search...

BRENDAⁱ	5.99.1.3 3352

EvolutionaryTraceⁱ	Q99XG5

Entry informationⁱ

Entry nameⁱ	GYRA_STAAN
Accessionⁱ	Q99XG5Primary (citable) accession number: Q99XG5
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
	Last sequence update:	June 1, 2001
	Last modified:	May 23, 2018
	This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

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UniProtKB - Q99XG5 (GYRA_STAAN)

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DNA gyrase subunit A

gyrA

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Motif

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ