We will be switching to the new UniProt website soon. Please explore and share your feedback.
Take me to the new website.
UniProtKB - Q99S77 (PTLCB_STAAN)
Protein
PTS system lactose-specific EIICB component
Gene
lacE
Organism
Staphylococcus aureus (strain N315)
Status
Functioni
The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II LacEF PTS system is involved in lactose transport.
By similarityCatalytic activityi
- lactose(out) + Nπ-phospho-L-histidyl-[protein] = L-histidyl-[protein] + lactose 6-phosphate(in)By similarityEC:2.7.1.207By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 474 | Phosphocysteine intermediate; for EIIB activityBy similarity | 1 |
GO - Molecular functioni
- kinase activity Source: UniProtKB-KW
- protein-N(PI)-phosphohistidine-lactose phosphotransferase system transporter activity Source: InterPro
GO - Biological processi
- phosphoenolpyruvate-dependent sugar phosphotransferase system Source: UniProtKB-KW
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Phosphotransferase system, Sugar transport, Transport |
Names & Taxonomyi
Protein namesi | Recommended name: PTS system lactose-specific EIICB componentBy similarityAlternative name(s): EIICB-LacBy similarity Short name: EII-LacBy similarity Including the following 2 domains: PTS system lactose-specific EIIC componentBy similarity Alternative name(s): Lactose permease IIC componentBy similarity Alternative name(s): Lactose-specific phosphotransferase enzyme IIB componentBy similarity |
Gene namesi | Name:lacEBy similarity Ordered Locus Names:SA1992 |
Organismi | Staphylococcus aureus (strain N315) |
Taxonomic identifieri | 158879 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell membrane PROSITE-ProRule annotationBy similarity; Multi-pass membrane protein PROSITE-ProRule annotationBy similarity
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 31 – 51 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 65 – 85 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 104 – 124 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 133 – 153 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 178 – 198 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 223 – 243 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 283 – 303 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 340 – 360 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 382 – 402 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000186587 | 1 – 570 | PTS system lactose-specific EIICB componentAdd BLAST | 570 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 474 | Phosphocysteine; by EIIAPROSITE-ProRule annotationBy similarity | 1 |
Keywords - PTMi
PhosphoproteinExpressioni
Inductioni
Induced by lactose, galactose and galactose-6-P. Repressed by glucose.By similarity
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 9 – 410 | PTS EIIC type-3PROSITE-ProRule annotationAdd BLAST | 402 | |
Domaini | 467 – 570 | PTS EIIB type-3PROSITE-ProRule annotationAdd BLAST | 104 |
Domaini
The EIIC type-3 domain forms the PTS system translocation channel and contains the specific substrate-binding site.PROSITE-ProRule annotation
The PTS EIIB type-3 domain is phosphorylated by phospho-EIIA on a cysteinyl residue. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the PTS EIIC type-3 domain.PROSITE-ProRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
HOGENOMi | CLU_029688_0_0_9 |
OMAi | ISAMPFM |
Family and domain databases
CDDi | cd05565, PTS_IIB_lactose, 1 hit |
InterProi | View protein in InterPro IPR004801, LacE IPR036095, PTS_EIIB-like_sf IPR003501, PTS_EIIB_2/3 IPR013012, PTS_EIIB_3 IPR003352, PTS_EIIC IPR004501, PTS_EIIC_3 IPR041713, PTS_IIB |
Pfami | View protein in Pfam PF02378, PTS_EIIC, 1 hit PF02302, PTS_IIB, 1 hit |
SUPFAMi | SSF52794, SSF52794, 1 hit |
TIGRFAMsi | TIGR00394, lac_pts_IIC, 1 hit TIGR00410, lacE, 1 hit TIGR00853, pts-lac, 1 hit |
PROSITEi | View protein in PROSITE PS51100, PTS_EIIB_TYPE_3, 1 hit PS51105, PTS_EIIC_TYPE_3, 1 hit |
i Sequence
Sequence statusi: Complete.
Q99S77-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MMQKLIAQIE KGKPFFEKLS RNIYLRAIRD GFISAMPVIL FSSIFLLIAY
60 70 80 90 100
VPNIFGFKWD KGMEAILMKP YNYTMGLVAF LVAGTTAKSL TDSFNRKLES
110 120 130 140 150
TNQINFISTM LAAMCGFLFL ASDPAKDGGF LSAFMGTKGL LTAFLSAFVT
160 170 180 190 200
VIVYNFCVKR NITIKMPKEV PPNISQVFKD LIPFSAVIII LYALDLVIRN
210 220 230 240 250
SFKSNVAEGI LKLFEPLFTA ADGWIGVTII FGAFALFWFV GIHGPSIVEP
260 270 280 290 300
AIAAITYANI EANFKLLQAG EHADKIITSG TQMFIVTFGG TGATLVVPFM
310 320 330 340 350
FMWMTKSKRN KAIGRASVVP TFFGVNEPIL FGAPLVLNPV FFIPFVLAPI
360 370 380 390 400
VNVWIFKLFV EVLGINSFSV NLPWTTPGPL GIIMGTGFGL WSFVLAITLI
410 420 430 440 450
VVDIIIYYPF LKVYDSEILD EEEGRKESNS DLKEKVAANF DTKKADSILA
460 470 480 490 500
ASGVSDDAAK ASNITEQTNV LVLCAGGGTS GLLANALNKA AEEYHVPVKA
510 520 530 540 550
AAGGYGAHMD IMKEYQLIIL APQVASNYED IKQDTDRLGI KLAKTQGAEY
560 570
IKLTRDGQAA LDFVQQQFEN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BA000018 Genomic DNA Translation: BAB43282.1 |
PIRi | A90015 |
Genome annotation databases
EnsemblBacteriai | BAB43282; BAB43282; BAB43282 |
KEGGi | sau:SA1992 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BA000018 Genomic DNA Translation: BAB43282.1 |
PIRi | A90015 |
3D structure databases
SMRi | Q99S77 |
ModBasei | Search... |
Genome annotation databases
EnsemblBacteriai | BAB43282; BAB43282; BAB43282 |
KEGGi | sau:SA1992 |
Phylogenomic databases
HOGENOMi | CLU_029688_0_0_9 |
OMAi | ISAMPFM |
Family and domain databases
CDDi | cd05565, PTS_IIB_lactose, 1 hit |
InterProi | View protein in InterPro IPR004801, LacE IPR036095, PTS_EIIB-like_sf IPR003501, PTS_EIIB_2/3 IPR013012, PTS_EIIB_3 IPR003352, PTS_EIIC IPR004501, PTS_EIIC_3 IPR041713, PTS_IIB |
Pfami | View protein in Pfam PF02378, PTS_EIIC, 1 hit PF02302, PTS_IIB, 1 hit |
SUPFAMi | SSF52794, SSF52794, 1 hit |
TIGRFAMsi | TIGR00394, lac_pts_IIC, 1 hit TIGR00410, lacE, 1 hit TIGR00853, pts-lac, 1 hit |
PROSITEi | View protein in PROSITE PS51100, PTS_EIIB_TYPE_3, 1 hit PS51105, PTS_EIIC_TYPE_3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PTLCB_STAAN | |
Accessioni | Q99S77Primary (citable) accession number: Q99S77 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | June 1, 2001 | |
Last modified: | February 23, 2022 | |
This is version 108 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |