Bhlhe41

Functionⁱ

Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA/B/G, NR1H3/LXRA, NR1H4 and VDR transactivation activity.5 Publications

Miscellaneous

Activity profiles and sleep recordings of transgenic mice carrying the mutation Arg-325 show increased vigilance time and less sleep time than control mice in a zeitgeber time- and sleep deprivation-dependent manner.

GO - Molecular functionⁱ

bHLH transcription factor binding
Source: BHF-UCL
Inferred from direct assayⁱ
- 12657651
DNA-binding transcription factor activity, RNA polymerase II-specific
Source: BHF-UCL
Inferred from direct assayⁱ
- 12397359
- 15147242
DNA-binding transcription repressor activity, RNA polymerase II-specific
Source: BHF-UCL
Inferred from direct assayⁱ
- 12397359
- 15147242
E-box binding
Source: BHF-UCL
Inferred from direct assayⁱ
histone deacetylase binding
Source: BHF-UCL
Inferred from direct assayⁱ
- 17487425
MRF binding
Source: BHF-UCL
Inferred from direct assayⁱ
- 12657651
- 17487425
protein heterodimerization activity
Source: BHF-UCL
Inferred from direct assayⁱ
- 17487425
protein homodimerization activity
Source: BHF-UCL
Inferred from physical interactionⁱ
- 17487425
proximal promoter DNA-binding transcription repressor activity, RNA polymerase II-specific
Source: BHF-UCL
Inferred from direct assayⁱ
- 12657651
- 17487425
RNA polymerase II activating transcription factor binding
Source: BHF-UCL
Inferred from direct assayⁱ
- 17487425
RNA polymerase II distal enhancer sequence-specific DNA binding
Source: BHF-UCL
Inferred from direct assayⁱ
- 15147242
RNA polymerase II proximal promoter sequence-specific DNA binding
Source: BHF-UCL
Inferred from direct assayⁱ
- 12657651
- 15147242
RNA polymerase II regulatory region sequence-specific DNA binding
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
sequence-specific DNA binding
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
sequence-specific double-stranded DNA binding
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
transcription corepressor activity
Source: UniProtKB
Inferred from direct assayⁱ
- 19786558
transcription factor binding
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cell differentiation
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
circadian regulation of gene expression
Source: BHF-UCL
Inferred from mutant phenotypeⁱ
- 18411297
circadian rhythm
Source: UniProtKB
Inferred from expression patternⁱ
- 19786558
negative regulation of myotube differentiation
Source: BHF-UCL
Inferred from direct assayⁱ
- 12657651
negative regulation of transcription, DNA-templated
Source: UniProtKB
Inferred from direct assayⁱ
- 12397359
- 24736997
negative regulation of transcription by competitive promoter binding
Source: BHF-UCL
Inferred from direct assayⁱ
- 12657651
negative regulation of transcription by RNA polymerase II
Source: BHF-UCL
Inferred from direct assayⁱ
- 12397359
Notch signaling pathway
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
regulation of neurogenesis
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
somitogenesis
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	DNA-binding, Repressor
Biological process	Biological rhythms, Transcription, Transcription regulation

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Class E basic helix-loop-helix protein 41 Short name: bHLHe41 Alternative name(s): Class B basic helix-loop-helix protein 3 Short name: bHLHb3 Differentially expressed in chondrocytes protein 2 Short name: mDEC2
Gene namesⁱ	Name:Bhlhe41 Synonyms:Bhlhb3, Dec2
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 6

Organism-specific databases

MGIⁱ	MGI:1930704 Bhlhe41

Keywords - Cellular componentⁱ

Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000127148	1 – 410	Class E basic helix-loop-helix protein 41Add BLAST		410

Amino acid modifications

Feature key	Position(s)	DescriptionActions
Cross-linkⁱ	31	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	121	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	240	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMⁱ

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q99PV5
PaxDbⁱ	Q99PV5
PRIDEⁱ	Q99PV5

PTM databases

iPTMnetⁱ	Q99PV5
PhosphoSitePlusⁱ	Q99PV5

Expressionⁱ

Tissue specificityⁱ

Expressed in skeletal muscle, brain and lung.

Inductionⁱ

Expressed in a circdadian manner in the liver with a peak at ZT10.1 Publication

Gene expression databases

Bgeeⁱ	ENSMUSG00000030256 Expressed in 97 organ(s), highest expression level in skeletal muscle tissue
ExpressionAtlasⁱ	Q99PV5 baseline and differential
Genevisibleⁱ	Q99PV5 MM

Interactionⁱ

Subunit structureⁱ

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
Cebpb	P28033	5	EBI-6143801,EBI-1029979
Ciart	Q3TQ03	2	EBI-6143801,EBI-16101489
HIF1A	Q16665	3	EBI-6143801,EBI-447269	From Homo sapiens.
PSMA4	P25789	2	EBI-6143801,EBI-359310	From Homo sapiens.

GO - Molecular functionⁱ

bHLH transcription factor binding
Source: BHF-UCL
Inferred from direct assayⁱ
- 12657651
histone deacetylase binding
Source: BHF-UCL
Inferred from direct assayⁱ
- 17487425
MRF binding
Source: BHF-UCL
Inferred from direct assayⁱ
- 12657651
- 17487425
protein heterodimerization activity
Source: BHF-UCL
Inferred from direct assayⁱ
- 17487425
protein homodimerization activity
Source: BHF-UCL
Inferred from physical interactionⁱ
- 17487425
RNA polymerase II activating transcription factor binding
Source: BHF-UCL
Inferred from direct assayⁱ
- 17487425
transcription factor binding
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	219755, 3 interactors
Database of interacting proteins More...DIPⁱ	DIP-57696N
IntActⁱ	Q99PV5, 25 interactors
Molecular INTeraction database More...MINTⁱ	Q99PV5
STRINGⁱ	10090.ENSMUSP00000032386

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q99PV5
SMRⁱ	Q99PV5
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	44 – 99	bHLHPROSITE-ProRule annotationAdd BLAST		56
Domainⁱ	131 – 166	OrangePROSITE-ProRule annotationAdd BLAST		36

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	321 – 373	Ala/Gly-richAdd BLAST		53

Phylogenomic databases

eggNOGⁱ	KOG4304 Eukaryota ENOG4111F0X LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00510000046898
HOGENOMⁱ	HOG000234381
InParanoidⁱ	Q99PV5
KEGG Orthology (KO) More...KOⁱ	K03730
OMAⁱ	LYMCKSK
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0D3I
PhylomeDBⁱ	Q99PV5
TreeFamⁱ	TF330859

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00083 HLH, 1 hit
Gene3Dⁱ	4.10.280.10, 1 hit
InterProⁱ	View protein in InterPro IPR011598 bHLH_dom IPR036638 HLH_DNA-bd_sf IPR003650 Orange_dom
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF07527 Hairy_orange, 1 hit PF00010 HLH, 1 hit
SMARTⁱ	View protein in SMART SM00353 HLH, 1 hit SM00511 ORANGE, 1 hit
SUPFAMⁱ	SSF47459 SSF47459, 1 hit
PROSITEⁱ	View protein in PROSITE PS50888 BHLH, 1 hit PS51054 ORANGE, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show all Align All

Q99PV5-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MDEGIPHLQE RQLLEHRDFI GLDYSSLYMC KPKRSLKRDD TKDTYKLPHR 
        60         70         80         90        100
LIEKKRRDRI NECIAQLKDL LPEHLKLTTL GHLEKAVVLE LTLKHLKALT 
       110        120        130        140        150
ALTEQQHQKI IALQNGERSL KSPVQADLDA FHSGFQTCAK EVLQYLARFE 
       160        170        180        190        200
SWTPREPRCA QLVSHLHAVA TQLLTPQVPS GRGSGRAPCS AGAAAASGPE 
       210        220        230        240        250
RVARCVPVIQ RTQPGTEPEH DTDTDSGYGG EAEQGRAAVK QEPPGDSSPA 
       260        270        280        290        300
PKRPKLEARG ALLGPEPALL GSLVALGGGA PFAQPAAAPF CLPFYLLSPS 
       310        320        330        340        350
AAAYVQPWLD KSGLDKYLYP AAAAPFPLLY PGIPAAAAAA AAAAFPCLSS 
       360        370        380        390        400
VLSPPPEKAG ATAGAPFLAH EVAPPGPLRP QHAHSRTHLP RAVNPESSQE 
       410
DATQPAKDAP

Length:410

Mass (Da):43,946

Last modified:June 1, 2001 - v1

Checksum:ⁱ40A87281B08E233D

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	D3Z2G6	D3Z2G6_MOUSE		Class E basic helix-loop-helix prot... Class E basic helix-loop-helix protein 41	Bhlhe41	131	Annotation score:

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AB044090 mRNA Translation: BAB21503.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS20697.1
PIRⁱ	JC7584
NCBI Reference Sequences More...RefSeqⁱ	NP_001258697.1, NM_001271768.1 NP_077789.1, NM_024469.2
UniGeneⁱ	Mm.154529

Genome annotation databases

Ensemblⁱ	ENSMUST00000032386; ENSMUSP00000032386; ENSMUSG00000030256
GeneIDⁱ	79362
KEGGⁱ	mmu:79362
UCSC genome browser More...UCSCⁱ	uc009ert.2 mouse

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q99PV5	BHLH transcriptional factor Dec2		MOUSE		410	UniRef100_Q99PV5
Bhlhe41 protein (Fragment)		MOUSE		193

Protein	Similar proteins		Species	Score	Length	Source
Q99PV5	Class E basic helix-loop-helix protein 41		RAT		410	UniRef90_Q99PV5
BHLH transcriptional factor Dec2		MOUSE		410
Uncharacterized protein		NEOLE		432
UPI000154C5DA		MUSCR		410
UPI00038C39E0		MICOH		409
+3

Protein	Similar proteins		Species	Score	Length	Source
Q99PV5	Class E basic helix-loop-helix protein 41		RAT		410	UniRef50_Q99PV5
BHLH transcriptional factor Dec2		MOUSE		410
Uncharacterized protein		NEOLE		432
Basic helix-loop-helix family, member e41		TAKRU		350
Basic helix-loop-helix family, member e41 (Fragment)		MUSPF		280
+8

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AB044090 mRNA Translation: BAB21503.1
CCDSⁱ	CCDS20697.1
PIRⁱ	JC7584
RefSeqⁱ	NP_001258697.1, NM_001271768.1 NP_077789.1, NM_024469.2
UniGeneⁱ	Mm.154529

3D structure databases

ProteinModelPortalⁱ	Q99PV5
SMRⁱ	Q99PV5
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	219755, 3 interactors
DIPⁱ	DIP-57696N
IntActⁱ	Q99PV5, 25 interactors
MINTⁱ	Q99PV5
STRINGⁱ	10090.ENSMUSP00000032386

PTM databases

iPTMnetⁱ	Q99PV5
PhosphoSitePlusⁱ	Q99PV5

Proteomic databases

MaxQBⁱ	Q99PV5
PaxDbⁱ	Q99PV5
PRIDEⁱ	Q99PV5

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000032386; ENSMUSP00000032386; ENSMUSG00000030256
GeneIDⁱ	79362
KEGGⁱ	mmu:79362
UCSCⁱ	uc009ert.2 mouse

Organism-specific databases

CTDⁱ	79365
MGIⁱ	MGI:1930704 Bhlhe41

Phylogenomic databases

eggNOGⁱ	KOG4304 Eukaryota ENOG4111F0X LUCA
GeneTreeⁱ	ENSGT00510000046898
HOGENOMⁱ	HOG000234381
InParanoidⁱ	Q99PV5
KOⁱ	K03730
OMAⁱ	LYMCKSK
OrthoDBⁱ	EOG091G0D3I
PhylomeDBⁱ	Q99PV5
TreeFamⁱ	TF330859

Miscellaneous databases

ChiTaRSⁱ	Bhlhe41 mouse
Protein Ontology More...PROⁱ	PR:Q99PV5
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000030256 Expressed in 97 organ(s), highest expression level in skeletal muscle tissue
ExpressionAtlasⁱ	Q99PV5 baseline and differential
Genevisibleⁱ	Q99PV5 MM

Family and domain databases

CDDⁱ	cd00083 HLH, 1 hit
Gene3Dⁱ	4.10.280.10, 1 hit
InterProⁱ	View protein in InterPro IPR011598 bHLH_dom IPR036638 HLH_DNA-bd_sf IPR003650 Orange_dom
Pfamⁱ	View protein in Pfam PF07527 Hairy_orange, 1 hit PF00010 HLH, 1 hit
SMARTⁱ	View protein in SMART SM00353 HLH, 1 hit SM00511 ORANGE, 1 hit
SUPFAMⁱ	SSF47459 SSF47459, 1 hit
PROSITEⁱ	View protein in PROSITE PS50888 BHLH, 1 hit PS51054 ORANGE, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	BHE41_MOUSE
Accessionⁱ	Q99PV5Primary (citable) accession number: Q99PV5
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	March 5, 2002
	Last sequence update:	June 1, 2001
	Last modified:	November 7, 2018
	This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q99PV5 (BHE41_MOUSE)

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Class E basic helix-loop-helix protein 41

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Compositional bias

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ