UniProtKB - Q99PV5 (BHE41_MOUSE)
Protein
Class E basic helix-loop-helix protein 41
Gene
Bhlhe41
Organism
Mus musculus (Mouse)
Status
Functioni
Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA/B/G, NR1H3/LXRA, NR1H4 and VDR transactivation activity. Inhibits HNF1A-mediated transactivation of CYP1A2, CYP2E1 AND CYP3A11 (PubMed:30555544).6 Publications
Miscellaneous
Activity profiles and sleep recordings of transgenic mice carrying the mutation Arg-325 show increased vigilance time and less sleep time than control mice in a zeitgeber time- and sleep deprivation-dependent manner.
GO - Molecular functioni
- bHLH transcription factor binding Source: BHF-UCL
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: BHF-UCL
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: BHF-UCL
- E-box binding Source: BHF-UCL
- histone deacetylase binding Source: BHF-UCL
- MRF binding Source: BHF-UCL
- protein heterodimerization activity Source: BHF-UCL
- protein homodimerization activity Source: BHF-UCL
- RNA polymerase II activating transcription factor binding Source: BHF-UCL
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: BHF-UCL
- transcription corepressor activity Source: MGI
GO - Biological processi
- anterior/posterior pattern specification Source: GO_Central
- circadian regulation of gene expression Source: BHF-UCL
- circadian rhythm Source: UniProtKB
- negative regulation of myotube differentiation Source: BHF-UCL
- negative regulation of transcription, DNA-templated Source: UniProtKB
- negative regulation of transcription by competitive promoter binding Source: BHF-UCL
- negative regulation of transcription by RNA polymerase II Source: BHF-UCL
- regulation of neurogenesis Source: GO_Central
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | DNA-binding, Repressor |
Biological process | Biological rhythms, Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Class E basic helix-loop-helix protein 41Short name: bHLHe41 Alternative name(s): Class B basic helix-loop-helix protein 3 Short name: bHLHb3 Differentially expressed in chondrocytes protein 2 Short name: mDEC2 |
Gene namesi | Name:Bhlhe41 Synonyms:Bhlhb3, Dec2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1930704, Bhlhe41 |
Subcellular locationi
Nucleus
- Nucleus PROSITE-ProRule annotation
Nucleus
- nucleoplasm Source: Reactome
- nucleus Source: GO_Central
Other locations
- transcription repressor complex Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000127148 | 1 – 410 | Class E basic helix-loop-helix protein 41Add BLAST | 410 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 31 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 121 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 240 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity |
Keywords - PTMi
Isopeptide bond, Ubl conjugationProteomic databases
MaxQBi | Q99PV5 |
PaxDbi | Q99PV5 |
PRIDEi | Q99PV5 |
ProteomicsDBi | 273682 |
PTM databases
iPTMneti | Q99PV5 |
PhosphoSitePlusi | Q99PV5 |
Expressioni
Tissue specificityi
Expressed in skeletal muscle, brain and lung.
Inductioni
Expressed in a circdadian manner in the liver with a peak at ZT10.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000030256, Expressed in retinal neural layer and 124 other tissues |
ExpressionAtlasi | Q99PV5, baseline and differential |
Genevisiblei | Q99PV5, MM |
Interactioni
Subunit structurei
Binary interactionsi
Hide detailsQ99PV5
GO - Molecular functioni
- bHLH transcription factor binding Source: BHF-UCL
- histone deacetylase binding Source: BHF-UCL
- MRF binding Source: BHF-UCL
- protein heterodimerization activity Source: BHF-UCL
- protein homodimerization activity Source: BHF-UCL
- RNA polymerase II activating transcription factor binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 219755, 3 interactors |
DIPi | DIP-57696N |
IntActi | Q99PV5, 25 interactors |
MINTi | Q99PV5 |
STRINGi | 10090.ENSMUSP00000032386 |
Miscellaneous databases
RNActi | Q99PV5, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 44 – 99 | bHLHPROSITE-ProRule annotationAdd BLAST | 56 | |
Domaini | 131 – 166 | OrangePROSITE-ProRule annotationAdd BLAST | 36 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 321 – 373 | Ala/Gly-richAdd BLAST | 53 |
Phylogenomic databases
eggNOGi | KOG4304, Eukaryota |
GeneTreei | ENSGT00940000161014 |
HOGENOMi | CLU_049895_0_0_1 |
InParanoidi | Q99PV5 |
OMAi | LYMCKSK |
OrthoDBi | 629563at2759 |
PhylomeDBi | Q99PV5 |
TreeFami | TF330859 |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR003650, Orange_dom |
Pfami | View protein in Pfam PF07527, Hairy_orange, 1 hit PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit SM00511, ORANGE, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit PS51054, ORANGE, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q99PV5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDEGIPHLQE RQLLEHRDFI GLDYSSLYMC KPKRSLKRDD TKDTYKLPHR
60 70 80 90 100
LIEKKRRDRI NECIAQLKDL LPEHLKLTTL GHLEKAVVLE LTLKHLKALT
110 120 130 140 150
ALTEQQHQKI IALQNGERSL KSPVQADLDA FHSGFQTCAK EVLQYLARFE
160 170 180 190 200
SWTPREPRCA QLVSHLHAVA TQLLTPQVPS GRGSGRAPCS AGAAAASGPE
210 220 230 240 250
RVARCVPVIQ RTQPGTEPEH DTDTDSGYGG EAEQGRAAVK QEPPGDSSPA
260 270 280 290 300
PKRPKLEARG ALLGPEPALL GSLVALGGGA PFAQPAAAPF CLPFYLLSPS
310 320 330 340 350
AAAYVQPWLD KSGLDKYLYP AAAAPFPLLY PGIPAAAAAA AAAAFPCLSS
360 370 380 390 400
VLSPPPEKAG ATAGAPFLAH EVAPPGPLRP QHAHSRTHLP RAVNPESSQE
410
DATQPAKDAP
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketD3Z2G6 | D3Z2G6_MOUSE | Class E basic helix-loop-helix prot... | Bhlhe41 | 131 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB044090 mRNA Translation: BAB21503.1 |
CCDSi | CCDS20697.1 |
PIRi | JC7584 |
RefSeqi | NP_001258697.1, NM_001271768.1 NP_077789.1, NM_024469.2 |
Genome annotation databases
Ensembli | ENSMUST00000032386; ENSMUSP00000032386; ENSMUSG00000030256 |
GeneIDi | 79362 |
KEGGi | mmu:79362 |
UCSCi | uc009ert.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB044090 mRNA Translation: BAB21503.1 |
CCDSi | CCDS20697.1 |
PIRi | JC7584 |
RefSeqi | NP_001258697.1, NM_001271768.1 NP_077789.1, NM_024469.2 |
3D structure databases
SMRi | Q99PV5 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 219755, 3 interactors |
DIPi | DIP-57696N |
IntActi | Q99PV5, 25 interactors |
MINTi | Q99PV5 |
STRINGi | 10090.ENSMUSP00000032386 |
PTM databases
iPTMneti | Q99PV5 |
PhosphoSitePlusi | Q99PV5 |
Proteomic databases
MaxQBi | Q99PV5 |
PaxDbi | Q99PV5 |
PRIDEi | Q99PV5 |
ProteomicsDBi | 273682 |
Protocols and materials databases
Antibodypediai | 24289, 420 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000032386; ENSMUSP00000032386; ENSMUSG00000030256 |
GeneIDi | 79362 |
KEGGi | mmu:79362 |
UCSCi | uc009ert.2, mouse |
Organism-specific databases
CTDi | 79365 |
MGIi | MGI:1930704, Bhlhe41 |
Phylogenomic databases
eggNOGi | KOG4304, Eukaryota |
GeneTreei | ENSGT00940000161014 |
HOGENOMi | CLU_049895_0_0_1 |
InParanoidi | Q99PV5 |
OMAi | LYMCKSK |
OrthoDBi | 629563at2759 |
PhylomeDBi | Q99PV5 |
TreeFami | TF330859 |
Miscellaneous databases
BioGRID-ORCSi | 79362, 0 hits in 52 CRISPR screens |
ChiTaRSi | Bhlhe41, mouse |
PROi | PR:Q99PV5 |
RNActi | Q99PV5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000030256, Expressed in retinal neural layer and 124 other tissues |
ExpressionAtlasi | Q99PV5, baseline and differential |
Genevisiblei | Q99PV5, MM |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR003650, Orange_dom |
Pfami | View protein in Pfam PF07527, Hairy_orange, 1 hit PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit SM00511, ORANGE, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit PS51054, ORANGE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BHE41_MOUSE | |
Accessioni | Q99PV5Primary (citable) accession number: Q99PV5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 5, 2002 |
Last sequence update: | June 1, 2001 | |
Last modified: | April 7, 2021 | |
This is version 150 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot