UniProtKB - Q99PS8 (HRG_RAT)
Histidine-rich glycoprotein
Hrg
Functioni
GO - Molecular functioni
- cysteine-type endopeptidase inhibitor activity Source: InterPro
- endopeptidase inhibitor activity Source: GO_Central
- heme binding Source: UniProtKB
- heparan sulfate proteoglycan binding Source: UniProtKB
- heparin binding Source: UniProtKB
- immunoglobulin binding Source: UniProtKB
- metal ion binding Source: UniProtKB
- serine-type endopeptidase inhibitor activity Source: RGD
- signaling receptor binding Source: UniProtKB
- zinc ion binding Source: UniProtKB
GO - Biological processi
- antimicrobial humoral immune response mediated by antimicrobial peptide Source: RGD
- cytolysis by host of symbiont cells Source: RGD
- defense response to fungus Source: UniProtKB
- fibrinolysis Source: UniProtKB-KW
- heme export Source: RGD
- heme transport Source: RGD
- negative regulation of angiogenesis Source: UniProtKB
- negative regulation of blood vessel endothelial cell migration Source: UniProtKB
- negative regulation of cell adhesion Source: UniProtKB
- negative regulation of cell adhesion mediated by integrin Source: UniProtKB
- negative regulation of cell growth Source: UniProtKB
- negative regulation of cell population proliferation Source: UniProtKB
- negative regulation of endopeptidase activity Source: GO_Central
- negative regulation of endothelial cell chemotaxis Source: UniProtKB
- negative regulation of fibrinolysis Source: RGD
- negative regulation of lamellipodium assembly Source: UniProtKB
- negative regulation of vascular endothelial growth factor signaling pathway Source: UniProtKB
- platelet activation Source: UniProtKB
- positive regulation of apoptotic process Source: UniProtKB
- positive regulation of blood vessel remodeling Source: UniProtKB
- positive regulation of focal adhesion assembly Source: UniProtKB
- positive regulation of immune response to tumor cell Source: UniProtKB
- regulation of actin cytoskeleton organization Source: UniProtKB
- regulation of blood coagulation Source: UniProtKB
- regulation of gene expression Source: UniProtKB
- regulation of peptidyl-tyrosine phosphorylation Source: UniProtKB
- regulation of platelet activation Source: UniProtKB
- regulation of protein-containing complex assembly Source: UniProtKB
- regulation of transcription from RNA polymerase II promoter in response to iron Source: RGD
- response to organic cyclic compound Source: RGD
Keywordsi
Molecular function | Heparin-binding |
Biological process | Blood coagulation, Fibrinolysis, Hemostasis |
Ligand | Copper, Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-RNO-114608, Platelet degranulation R-RNO-75205, Dissolution of Fibrin Clot |
Protein family/group databases
MEROPSi | I25.022 |
Names & Taxonomyi
Protein namesi | Recommended name: Histidine-rich glycoproteinAlternative name(s): Histidine-proline-rich glycoprotein Short name: HPRG Histidine-rich glycoprotein 1 Short name: HRG1 |
Gene namesi | Name:Hrg Synonyms:Hrg1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 619808, Hrg |
Subcellular locationi
Extracellular region or secreted
- Secreted 1 Publication
Endosome
- endolysosome Source: RGD
Extracellular region or secreted
- extracellular region Source: RGD
Lysosome
- endolysosome Source: RGD
- phagolysosome membrane Source: RGD
Other locations
- cell surface Source: RGD
- vesicle Source: RGD
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 18 | Sequence analysisAdd BLAST | 18 | |
ChainiPRO_0000408508 | 19 – 525 | Histidine-rich glycoproteinAdd BLAST | 507 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 24 ↔ 504 | By similarity | ||
Disulfide bondi | 78 ↔ 89 | By similarity | ||
Disulfide bondi | 103 ↔ 124 | By similarity | ||
Glycosylationi | 112 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 123 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 145 | PhosphoserineCombined sources | 1 | |
Glycosylationi | 200 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 201 ↔ 414 | By similarity | ||
Disulfide bondi | 216 ↔ 239 | By similarity | ||
Glycosylationi | 322 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 330 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 438 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 439 – 440 | Cleavage; by plasminBy similarity | 2 |
Keywords - PTMi
Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
PaxDbi | Q99PS8 |
PRIDEi | Q99PS8 |
PTM databases
GlyGeni | Q99PS8, 5 sites |
iPTMneti | Q99PS8 |
PhosphoSitePlusi | Q99PS8 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSRNOG00000001809, Expressed in liver and 17 other tissues |
ExpressionAtlasi | Q99PS8, baseline and differential |
Genevisiblei | Q99PS8, RN |
Interactioni
Subunit structurei
Interacts with THBS1 (via the TSP type I repeats); the interaction blocks the antiangiogenic effect of THBS1 with CD36.
Interacts with HPSE; the interaction is enhanced at acidic pH, partially inhibits binding of HPSE to cell surface receptors and modulates its enzymatic activity.
Interacts (via the HRR domain) with TMP1; the interaction partially mediates the antiangiogenic properties of HRG.
Interacts with kappa and lambda light chains of IgG molecules.
Interacts with ATP5F1A; the interaction occurs on the surface of T-cells and alters their cell morphology in concert with CONA. Binds IgG molecules containing kappa and lambda light chains and inhibits the formation of insoluble immunoglobulin complexes.
Interacts with F12; the interaction, which is enhanced in the presence of zinc ions and inhibited by heparin-binding to HRG, inhibits factor XII autoactivation and contact-initiated coagulation (By similarity).
Interacts with PLG (via its Kringle domains); the interaction tethers PLG to the cell surface and enhances its activation.
Interacts (via the HRR domain) with TPM1; the interaction appears to contribute to the antiangiogenic properties of the HRR domain.
Interacts with THBS2; the interaction blocks the antiangiogenic effect of THBS2 with CD36 (By similarity).
By similarityGO - Molecular functioni
- heparan sulfate proteoglycan binding Source: UniProtKB
- signaling receptor binding Source: UniProtKB
Protein-protein interaction databases
IntActi | Q99PS8, 6 interactors |
STRINGi | 10116.ENSRNOP00000049290 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 19 – 122 | Cystatin 1Add BLAST | 104 | |
Domaini | 135 – 240 | Cystatin 2Add BLAST | 106 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 41 – 84 | Interaction with ATP5F1ABy similarityAdd BLAST | 44 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 267 – 312 | Pro-richAdd BLAST | 46 | |
Compositional biasi | 337 – 497 | His/Pro-rich (HRR)Add BLAST | 161 | |
Compositional biasi | 462 – 497 | Pro-richAdd BLAST | 36 |
Domaini
Keywords - Domaini
Repeat, SignalPhylogenomic databases
eggNOGi | ENOG502S50D, Eukaryota |
GeneTreei | ENSGT00950000182930 |
HOGENOMi | CLU_575637_0_0_1 |
InParanoidi | Q99PS8 |
OrthoDBi | 715844at2759 |
PhylomeDBi | Q99PS8 |
TreeFami | TF333729 |
Family and domain databases
CDDi | cd00042, CY, 1 hit |
InterProi | View protein in InterPro IPR000010, Cystatin_dom |
SMARTi | View protein in SMART SM00043, CY, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MKVLTTALLL VTLQCSHALS PTNCDASKPL AEKVLDLINK GRRSGYTFQL
60 70 80 90 100
LRVSDAHLDR VETATIYYLV LDVVESDCWV LSTKAQDECL PAMRTSEVVI
110 120 130 140 150
GQCKVIATRY SNESQDLSVN GYNCTMRSVS SAYINTKDSP VLVDSFEDSE
160 170 180 190 200
PYRKLARKAL DKYKAENGDF ASFRVERAER VIRMRGGERT SYFIEFSVRN
210 220 230 240 250
CSTQHFPRHP PVFGLCRVVL TYSTEASDLE TPEYTDLICE VFNTEDLKNR
260 270 280 290 300
SDMKPHRGHE HPHCDKHLCK LSGPRDHHHT HKTHEIGCPP PPEGKDNSDR
310 320 330 340 350
PPLQEGALPQ MLPGHSGPSG TNRSHRPPHN HSCNEHPCHG QHPHGHHPHG
360 370 380 390 400
QHPHGHHPHG QHPHGHHPHG QHPHGHHPHG QHPHGHHPHG HHPHGDHPHG
410 420 430 440 450
HHPHGHDFLD YGPCDPPSNS QELKGQYHRG HGPPHGHSRK RGPGKGLFPF
460 470 480 490 500
HQRQIGYVYR LPPLNVGEVL TPPEANFPIF SLPNCNRPPQ PEIRPFPQTA
510 520
SKSCPGKFEG KFPQVSNFFE HTPPK
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2K3G0 | A0A0G2K3G0_RAT | Histidine-rich glycoprotein | Hrg rCG_36700 | 536 | Annotation score: | ||
A0A0G2K9Y5 | A0A0G2K9Y5_RAT | Histidine-rich glycoprotein | Hrg | 515 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 6 | T → A in AAG28417 (PubMed:10849117).Curated | 1 | |
Sequence conflicti | 74 | V → I in AAG28417 (PubMed:10849117).Curated | 1 | |
Sequence conflicti | 74 | V → I in BAB33093 (Ref. 2) Curated | 1 | |
Sequence conflicti | 93 – 98 | MRTSEV → RWTSEI in AAG28417 (PubMed:10849117).Curated | 6 | |
Sequence conflicti | 93 – 98 | MRTSEV → RWTSEI in BAB33093 (Ref. 2) Curated | 6 | |
Sequence conflicti | 133 – 134 | YI → LS in AAG28417 (PubMed:10849117).Curated | 2 | |
Sequence conflicti | 133 – 134 | YI → LS in BAB33093 (Ref. 2) Curated | 2 | |
Sequence conflicti | 143 – 145 | VDS → FDF in AAG28417 (PubMed:10849117).Curated | 3 | |
Sequence conflicti | 143 – 145 | VDS → FDF in BAB33093 (Ref. 2) Curated | 3 | |
Sequence conflicti | 165 | A → E in AAG28417 (PubMed:10849117).Curated | 1 | |
Sequence conflicti | 165 | A → E in BAB33093 (Ref. 2) Curated | 1 | |
Sequence conflicti | 172 | S → L in AAG28417 (PubMed:10849117).Curated | 1 | |
Sequence conflicti | 184 | M → G in AAG28417 (PubMed:10849117).Curated | 1 | |
Sequence conflicti | 184 | M → G in BAB33093 (Ref. 2) Curated | 1 | |
Sequence conflicti | 191 | S → N in AAG28417 (PubMed:10849117).Curated | 1 | |
Sequence conflicti | 211 | P → L in AAG28417 (PubMed:10849117).Curated | 1 | |
Sequence conflicti | 215 | L → F in AAG28417 (PubMed:10849117).Curated | 1 | |
Sequence conflicti | 215 | L → F in BAB33093 (Ref. 2) Curated | 1 | |
Sequence conflicti | 218 – 224 | VVLTYST → ALLSYSI in AAG28417 (PubMed:10849117).Curated | 7 | |
Sequence conflicti | 218 – 224 | VVLTYST → ALLSYSI in BAB33093 (Ref. 2) Curated | 7 | |
Sequence conflicti | 237 – 238 | LI → VT in AAG28417 (PubMed:10849117).Curated | 2 | |
Sequence conflicti | 237 – 238 | LI → VT in BAB33093 (Ref. 2) Curated | 2 | |
Sequence conflicti | 242 | F → V in AAG28417 (PubMed:10849117).Curated | 1 | |
Sequence conflicti | 242 | F → V in BAB33093 (Ref. 2) Curated | 1 | |
Sequence conflicti | 329 | H → R in AAG28417 (PubMed:10849117).Curated | 1 | |
Sequence conflicti | 339 – 348 | Missing in AAG28417 (PubMed:10849117).Curated | 10 | |
Sequence conflicti | 339 – 348 | Missing in BAB33093 (Ref. 2) Curated | 10 | |
Sequence conflicti | 374 | H → R in AAG28417 (PubMed:10849117).Curated | 1 | |
Sequence conflicti | 381 – 385 | QHPHG → HHLHR in BAB33093 (Ref. 2) Curated | 5 | |
Sequence conflicti | 384 | H → R in AAG28417 (PubMed:10849117).Curated | 1 | |
Sequence conflicti | 421 – 425 | Missing in AAG28417 (PubMed:10849117).Curated | 5 | |
Sequence conflicti | 429 | R → Q in AAG28417 (PubMed:10849117).Curated | 1 | |
Sequence conflicti | 479 | I → S in BAB33093 (Ref. 2) Curated | 1 | |
Sequence conflicti | 494 | R → Q in BAB33093 (Ref. 2) Curated | 1 | |
Sequence conflicti | 510 | G → S in BAB33093 (Ref. 2) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF194029 mRNA Translation: AAG28417.1 AB055895 mRNA Translation: BAB33092.1 AB055896 mRNA Translation: BAB33093.1 BC089779 mRNA Translation: AAH89779.1 |
RefSeqi | NP_001316829.1, NM_001329900.1 NP_596919.1, NM_133428.2 |
Genome annotation databases
Ensembli | ENSRNOT00000047595; ENSRNOP00000049290; ENSRNOG00000001809 |
GeneIDi | 171016 681544 |
KEGGi | rno:171016 rno:681544 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF194029 mRNA Translation: AAG28417.1 AB055895 mRNA Translation: BAB33092.1 AB055896 mRNA Translation: BAB33093.1 BC089779 mRNA Translation: AAH89779.1 |
RefSeqi | NP_001316829.1, NM_001329900.1 NP_596919.1, NM_133428.2 |
3D structure databases
SMRi | Q99PS8 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | Q99PS8, 6 interactors |
STRINGi | 10116.ENSRNOP00000049290 |
Protein family/group databases
MEROPSi | I25.022 |
PTM databases
GlyGeni | Q99PS8, 5 sites |
iPTMneti | Q99PS8 |
PhosphoSitePlusi | Q99PS8 |
Proteomic databases
PaxDbi | Q99PS8 |
PRIDEi | Q99PS8 |
Genome annotation databases
Ensembli | ENSRNOT00000047595; ENSRNOP00000049290; ENSRNOG00000001809 |
GeneIDi | 171016 681544 |
KEGGi | rno:171016 rno:681544 |
Organism-specific databases
CTDi | 3273 |
RGDi | 619808, Hrg |
Phylogenomic databases
eggNOGi | ENOG502S50D, Eukaryota |
GeneTreei | ENSGT00950000182930 |
HOGENOMi | CLU_575637_0_0_1 |
InParanoidi | Q99PS8 |
OrthoDBi | 715844at2759 |
PhylomeDBi | Q99PS8 |
TreeFami | TF333729 |
Enzyme and pathway databases
Reactomei | R-RNO-114608, Platelet degranulation R-RNO-75205, Dissolution of Fibrin Clot |
Miscellaneous databases
PROi | PR:Q99PS8 |
Gene expression databases
Bgeei | ENSRNOG00000001809, Expressed in liver and 17 other tissues |
ExpressionAtlasi | Q99PS8, baseline and differential |
Genevisiblei | Q99PS8, RN |
Family and domain databases
CDDi | cd00042, CY, 1 hit |
InterProi | View protein in InterPro IPR000010, Cystatin_dom |
SMARTi | View protein in SMART SM00043, CY, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HRG_RAT | |
Accessioni | Q99PS8Primary (citable) accession number: Q99PS8 Secondary accession number(s): D3ZJN0, Q99PS7, Q9ESB2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 31, 2011 |
Last sequence update: | June 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 115 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |