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UniProtKB - Q99PR0 (POMT1_RAT)
Protein
Protein O-mannosyl-transferase 1
Gene
Pomt1
Organism
Rattus norvegicus (Rat)
Status
Functioni
Transfers mannosyl residues to the hydroxyl group of serine or threonine residues. Coexpression of both POMT1 and POMT2 is necessary for enzyme activity, expression of either POMT1 or POMT2 alone is insufficient. Essentially dedicated to O-mannosylation of alpha-DAG1 and few other proteins but not of cadherins and protocaherins.
By similarityCatalytic activityi
- dolichyl β-D-mannosyl phosphate + L-seryl-[protein] = 3-O-(α-D-mannosyl)-L-seryl-[protein] + dolichyl phosphate + H+By similarityEC:2.4.1.109By similarity
- dolichyl β-D-mannosyl phosphate + L-threonyl-[protein] = 3-O-(α-D-mannosyl)-L-threonyl-[protein] + dolichyl phosphate + H+By similarityEC:2.4.1.109By similarity
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
GO - Molecular functioni
- dolichyl-phosphate-mannose-protein mannosyltransferase activity Source: UniProtKB-EC
- mannosyltransferase activity Source: UniProtKB
GO - Biological processi
- extracellular matrix organization Source: RGD
- positive regulation of protein O-linked glycosylation Source: RGD
- protein O-linked mannosylation Source: UniProtKB
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Enzyme and pathway databases
BRENDAi | 2.4.1.109, 5301 |
UniPathwayi | UPA00378 |
Protein family/group databases
CAZyi | GT39, Glycosyltransferase Family 39 |
Names & Taxonomyi
Protein namesi | Recommended name: Protein O-mannosyl-transferase 1 (EC:2.4.1.109)Alternative name(s): Dolichyl-phosphate-mannose--protein mannosyltransferase 1 |
Gene namesi | Name:Pomt1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 620078, Pomt1 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein By similarity
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB
- sarcoplasmic reticulum Source: RGD
Other locations
- acrosomal vesicle Source: RGD
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 30 – 50 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 90 – 110 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 121 – 141 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 176 – 196 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 205 – 225 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 228 – 248 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 267 – 287 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 597 – 617 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 636 – 656 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 660 – 680 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000121486 | 1 – 747 | Protein O-mannosyl-transferase 1Add BLAST | 747 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 435 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 471 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 539 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
PaxDbi | Q99PR0 |
PTM databases
GlyGeni | Q99PR0, 3 sites, 2 N-linked glycans (1 site) |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000010477, Expressed in testis and 21 other tissues |
ExpressionAtlasi | Q99PR0, baseline and differential |
Genevisiblei | Q99PR0, RN |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 318 – 381 | MIR 1PROSITE-ProRule annotationAdd BLAST | 64 | |
Domaini | 392 – 449 | MIR 2PROSITE-ProRule annotationAdd BLAST | 58 | |
Domaini | 453 – 513 | MIR 3PROSITE-ProRule annotationAdd BLAST | 61 |
Sequence similaritiesi
Belongs to the glycosyltransferase 39 family.Curated
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3359, Eukaryota |
GeneTreei | ENSGT00940000158049 |
InParanoidi | Q99PR0 |
OMAi | FWTQNDT |
OrthoDBi | 203029at2759 |
PhylomeDBi | Q99PR0 |
TreeFami | TF300552 |
Family and domain databases
InterProi | View protein in InterPro IPR027005, GlyclTrfase_39-like IPR003342, Glyco_trans_39/83 IPR036300, MIR_dom_sf IPR016093, MIR_motif IPR032421, PMT_4TMC |
PANTHERi | PTHR10050, PTHR10050, 1 hit |
Pfami | View protein in Pfam PF02815, MIR, 1 hit PF02366, PMT, 1 hit PF16192, PMT_4TMC, 1 hit |
SMARTi | View protein in SMART SM00472, MIR, 3 hits |
SUPFAMi | SSF82109, SSF82109, 1 hit |
PROSITEi | View protein in PROSITE PS50919, MIR, 3 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q99PR0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGNRSMGRED TLGVLPSLLF CKMLRFLKRP LVVTIDINLN LVALTVLGLL
60 70 80 90 100
TRLWQLSYPR AVVFDEVYYG QYISFYMKRV FFLDDSGPPF GHMLLALGGW
110 120 130 140 150
LGGFDGNFLW NRIGAEYSSN VPVWSLRLLP ALAGALSVPM AYQIVLELHF
160 170 180 190 200
SHCTAMGAAL LMLIENALIT QSRLMLLESI LIFFNLLAVL SYLKFFNSQT
210 220 230 240 250
HSPFSVHWWL WLMLTGVSCS CAVGIKYMGI FTYLLVLSIA AVHAWHLIGD
260 270 280 290 300
QTLSNICVLS HLLARAVALL VVPVFLYLLF FYVHLMLLYR SGPHDQIMSS
310 320 330 340 350
AFQASLEGGL ARITQGQPLE VAFGSQVTLK SVSGKPLPCW LHSHKNTYPM
360 370 380 390 400
IYENGRGSSH QQQVTCYPFK DINNWWIVKD PGRHQLVVNN PPRPVRHGDI
410 420 430 440 450
VQLVHGMTTR LLNTHDVAAP LSPHSQEVSC YIDYNISMPA QNLWKLDIVN
460 470 480 490 500
RESNQDTWKT ILSEVRFVHV NTSAILKLSG AHLPDWGFRQ LEVVGEKLSL
510 520 530 540 550
GPHESMVWNV EEHRYGRGHE QKERELELHS PTQHDISRNL SFMARFSELQ
560 570 580 590 600
WKMLTLKNED LEHQYSSTPL EWLTLDTNIA YWLHPRTSAQ IHLLGNIVIW
610 620 630 640 650
TSASLATVAY TLLFFWYLLR RRRNICDLPE DAWSHWVLAG ALCIGGWALN
660 670 680 690 700
YLPFFLMERM LFLYHYLPAL TFQILLLPIV MQHASDHLCR SQLQRNVFSA
710 720 730 740
LVVAWYSSAC HVSNMLRPLT YGDTSLSPGE LRALRWKDSW DILIRKY
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2K523 | A0A0G2K523_RAT | Dolichyl-phosphate-mannose--protein... | Pomt1 | 703 | Annotation score: |
Sequence cautioni
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF192388 mRNA Translation: AAG53461.1 BC070912 mRNA Translation: AAH70912.1 Sequence problems. |
RefSeqi | NP_445858.1, NM_053406.2 |
Genome annotation databases
Ensembli | ENSRNOT00000015214; ENSRNOP00000015214; ENSRNOG00000010477 |
GeneIDi | 84430 |
KEGGi | rno:84430 |
UCSCi | RGD:620078, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF192388 mRNA Translation: AAG53461.1 BC070912 mRNA Translation: AAH70912.1 Sequence problems. |
RefSeqi | NP_445858.1, NM_053406.2 |
3D structure databases
SMRi | Q99PR0 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000015214 |
Protein family/group databases
CAZyi | GT39, Glycosyltransferase Family 39 |
PTM databases
GlyGeni | Q99PR0, 3 sites, 2 N-linked glycans (1 site) |
Proteomic databases
PaxDbi | Q99PR0 |
Genome annotation databases
Ensembli | ENSRNOT00000015214; ENSRNOP00000015214; ENSRNOG00000010477 |
GeneIDi | 84430 |
KEGGi | rno:84430 |
UCSCi | RGD:620078, rat |
Organism-specific databases
CTDi | 10585 |
RGDi | 620078, Pomt1 |
Phylogenomic databases
eggNOGi | KOG3359, Eukaryota |
GeneTreei | ENSGT00940000158049 |
InParanoidi | Q99PR0 |
OMAi | FWTQNDT |
OrthoDBi | 203029at2759 |
PhylomeDBi | Q99PR0 |
TreeFami | TF300552 |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
BRENDAi | 2.4.1.109, 5301 |
Miscellaneous databases
PROi | PR:Q99PR0 |
Gene expression databases
Bgeei | ENSRNOG00000010477, Expressed in testis and 21 other tissues |
ExpressionAtlasi | Q99PR0, baseline and differential |
Genevisiblei | Q99PR0, RN |
Family and domain databases
InterProi | View protein in InterPro IPR027005, GlyclTrfase_39-like IPR003342, Glyco_trans_39/83 IPR036300, MIR_dom_sf IPR016093, MIR_motif IPR032421, PMT_4TMC |
PANTHERi | PTHR10050, PTHR10050, 1 hit |
Pfami | View protein in Pfam PF02815, MIR, 1 hit PF02366, PMT, 1 hit PF16192, PMT_4TMC, 1 hit |
SMARTi | View protein in SMART SM00472, MIR, 3 hits |
SUPFAMi | SSF82109, SSF82109, 1 hit |
PROSITEi | View protein in PROSITE PS50919, MIR, 3 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | POMT1_RAT | |
Accessioni | Q99PR0Primary (citable) accession number: Q99PR0 Secondary accession number(s): Q6IRI2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 20, 2003 |
Last sequence update: | June 1, 2001 | |
Last modified: | February 23, 2022 | |
This is version 136 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families