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Entry version 147 (12 Aug 2020)
Sequence version 2 (07 Dec 2004)
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Protein

E3 ubiquitin-protein ligase TRIM33

Gene

Trim33

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway (By similarity). May act as a transcriptional repressor (By similarity). Inhibits the transcriptional response to TGF-beta/BMP signaling cascade (By similarity). Plays a role in the control of cell proliferation (By similarity). Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor. Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade) (By similarity).By similarity1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

  • S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. EC:2.3.2.27

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section specifies the position(s) and type(s) of zinc fingers within the protein.<p><a href='/help/zn_fing' target='_top'>More...</a></p>Zinc fingeri141 – 201RING-typePROSITE-ProRule annotationAdd BLAST61
Zinc fingeri228 – 275B box-type 1PROSITE-ProRule annotationAdd BLAST48
Zinc fingeri287 – 328B box-type 2PROSITE-ProRule annotationAdd BLAST42
Zinc fingeri902 – 949PHD-typePROSITE-ProRule annotationAdd BLAST48

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionDNA-binding, Repressor, Transferase
Biological processTranscription, Transcription regulation, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-MMU-2173795, Downregulation of SMAD2/3:SMAD4 transcriptional activity

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...
UniPathwayi
UPA00143

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
E3 ubiquitin-protein ligase TRIM33 (EC:2.3.2.27)
Alternative name(s):
Ectodermin homolog
RING-type E3 ubiquitin transferase TRIM33Curated
Transcription intermediary factor 1-gamma
Short name:
TIF1-gamma
Tripartite motif-containing protein 33
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:Trim33
Synonyms:Kiaa1113
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMus musculus (Mouse)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri10090 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000589 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Unplaced

Organism-specific databases

Mouse genome database (MGD) from Mouse Genome Informatics (MGI)

More...
MGIi
MGI:2137357, Trim33

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000563961 – 1142E3 ubiquitin-protein ligase TRIM33Add BLAST1142

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM / Processing</a> section describes <strong>covalent linkages</strong> of various types formed <strong>between two proteins (interchain cross-links)</strong> or <strong>between two parts of the same protein (intrachain cross-links)</strong>, except the disulfide bonds that are annotated in the <a href="http://www.uniprot.org/manual/disulfid">'Disulfide bond'</a> subsection.<p><a href='/help/crosslnk' target='_top'>More...</a></p>Cross-linki345Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki350Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki497Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki520Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei531Asymmetric dimethylarginine; alternateCombined sources1
Modified residuei531Omega-N-methylarginine; alternateCombined sources1
Cross-linki543Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei551Omega-N-methylarginineBy similarity1
Modified residuei593Asymmetric dimethylarginineCombined sources1
Modified residuei607Asymmetric dimethylarginine; alternateCombined sources1
Modified residuei607Omega-N-methylarginine; alternateBy similarity1
Modified residuei614Asymmetric dimethylarginineCombined sources1
Modified residuei620Asymmetric dimethylarginineCombined sources1
Modified residuei778N6-acetyllysine; alternateCombined sources1
Cross-linki778Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei784N6-acetyllysine; alternateCombined sources1
Cross-linki784Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Cross-linki789Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki791Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Cross-linki791Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei808N6-acetyllysine; alternateCombined sources1
Cross-linki808Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Cross-linki808Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Cross-linki811Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei818PhosphoserineCombined sources1
Modified residuei830PhosphothreonineCombined sources1
Cross-linki876Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei877PhosphoserineBy similarity1
Modified residuei966N6-acetyllysineCombined sources1
Modified residuei968N6-acetyllysine; alternateCombined sources1
Cross-linki968Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Cross-linki1022Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki1058Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei1066PhosphothreonineBy similarity1
Cross-linki1072Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei1117PhosphothreonineBy similarity1
Modified residuei1120PhosphoserineBy similarity1
Cross-linki1133Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei1134PhosphoserineBy similarity1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Sumoylated with SUMO1.1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

Encyclopedia of Proteome Dynamics

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EPDi
Q99PP7

jPOST - Japan Proteome Standard Repository/Database

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jPOSTi
Q99PP7

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
Q99PP7

PRoteomics IDEntifications database

More...
PRIDEi
Q99PP7

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
Q99PP7

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...
PhosphoSitePlusi
Q99PP7

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Ubiquitous with high level in testis.

<p>This subsection of the 'Expression' section provides information on the expression of the gene product at various stages of a cell, tissue or organism development. By default, the information is derived from experiments at the mRNA level, unless specified 'at the protein level'.<p><a href='/help/developmental_stage' target='_top'>More...</a></p>Developmental stagei

Expressed in round hematopoietic cells in yolk sac blood islands at 8.5 dpc. Expressed uniformly at 10.5 dpc. Expressed in the brain, spinal cord, neuroepithelium and in spinal ganglia at 12.5 dpc. Expressed in brain, spinal cord, developing epithelia of the lung, stomach, intestine, outer region of the developing kidney, liver, brown fat tissue, skeletal muscle, developing craniofacial region, thymus, cochlear and pharyngeal epithelia and olfactory and respiratory epithelia at 16.5 dpc.2 Publications

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homooligomer and heterooligomer with TRIM24 and TRIM28 family members (By similarity).

Interacts with SMAD4 in unstimulated cells (By similarity).

Found in a complex with SMAD2 and SMAD3 upon addition of TGF-beta (By similarity).

Interacts with SMAD2 and SMAD3 (By similarity).

Interacts with SMAD4 under basal and induced conditions and, upon TGF-beta signaling, with activated SMAD2.

Forms a ternary complex with SMAD4 and SMAD2 upon TGF-beta signaling (By similarity).

By similarity

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...
BioGRIDi
220448, 5 interactors

CORUM comprehensive resource of mammalian protein complexes

More...
CORUMi
Q99PP7

Database of interacting proteins

More...
DIPi
DIP-59674N

Protein interaction database and analysis system

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IntActi
Q99PP7, 6 interactors

Molecular INTeraction database

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MINTi
Q99PP7

STRING: functional protein association networks

More...
STRINGi
10090.ENSMUSP00000029444

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...
RNActi
Q99PP7, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

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SMRi
Q99PP7

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini989 – 1061BromoPROSITE-ProRule annotationAdd BLAST73

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1 – 163Necessary for E3 ubiquitin-protein ligase activity and repression of SMAD4 signaling and transcriptional repressionBy similarityAdd BLAST163
Regioni315 – 417Necessary for oligomerizationBy similarityAdd BLAST103

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and domains' section denotes the positions of regions of coiled coil within the protein.<p><a href='/help/coiled' target='_top'>More...</a></p>Coiled coili315 – 417Sequence analysisAdd BLAST103

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi13 – 18Poly-Gly6
Compositional biasi20 – 122Ala-richAdd BLAST103
Compositional biasi524 – 636Gln-richAdd BLAST113
Compositional biasi708 – 785Ser-richAdd BLAST78

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the TRIM/RBCC family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri141 – 201RING-typePROSITE-ProRule annotationAdd BLAST61
Zinc fingeri228 – 275B box-type 1PROSITE-ProRule annotationAdd BLAST48
Zinc fingeri287 – 328B box-type 2PROSITE-ProRule annotationAdd BLAST42
Zinc fingeri902 – 949PHD-typePROSITE-ProRule annotationAdd BLAST48

Keywords - Domaini

Bromodomain, Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

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eggNOGi
KOG2177, Eukaryota

InParanoid: Eukaryotic Ortholog Groups

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InParanoidi
Q99PP7

KEGG Orthology (KO)

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KOi
K08883

Database of Orthologous Groups

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OrthoDBi
756911at2759

Database for complete collections of gene phylogenies

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PhylomeDBi
Q99PP7

Family and domain databases

Conserved Domains Database

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CDDi
cd00021, BBOX, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

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Gene3Di
1.20.920.10, 1 hit
3.30.40.10, 2 hits

Integrated resource of protein families, domains and functional sites

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InterProi
View protein in InterPro
IPR003649, Bbox_C
IPR001487, Bromodomain
IPR036427, Bromodomain-like_sf
IPR019786, Zinc_finger_PHD-type_CS
IPR027370, Znf-RING_LisH
IPR000315, Znf_B-box
IPR011011, Znf_FYVE_PHD
IPR001965, Znf_PHD
IPR019787, Znf_PHD-finger
IPR001841, Znf_RING
IPR013083, Znf_RING/FYVE/PHD
IPR017907, Znf_RING_CS

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00439, Bromodomain, 1 hit
PF00628, PHD, 1 hit
PF00643, zf-B_box, 1 hit
PF13445, zf-RING_UBOX, 1 hit

Protein Motif fingerprint database; a protein domain database

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PRINTSi
PR00503, BROMODOMAIN

Simple Modular Architecture Research Tool; a protein domain database

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SMARTi
View protein in SMART
SM00502, BBC, 1 hit
SM00336, BBOX, 2 hits
SM00297, BROMO, 1 hit
SM00249, PHD, 2 hits
SM00184, RING, 2 hits

Superfamily database of structural and functional annotation

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SUPFAMi
SSF47370, SSF47370, 1 hit
SSF57903, SSF57903, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS50014, BROMODOMAIN_2, 1 hit
PS50119, ZF_BBOX, 2 hits
PS01359, ZF_PHD_1, 1 hit
PS50016, ZF_PHD_2, 1 hit
PS00518, ZF_RING_1, 1 hit
PS50089, ZF_RING_2, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (2+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 2 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform. This section is only present in reviewed entries, i.e. in UniProtKB/Swiss-Prot.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All

Isoform Alpha (identifier: Q99PP7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAENKGGGEA ESGGGGSGSA PVTAGAAGPT AQEAEPPLAA VLVEEEEEEG
60 70 80 90 100
GRAGAEGGAA GPDDGGVAAA SSSSAPAASV PAASVGSAVP GGAASTPAPA
110 120 130 140 150
AAPAPAPAPA PAPAPAPAPA PAPGSSSGPP LGPPASLLDT CAVCQQSLQS
160 170 180 190 200
RREAEPKLLP CLHSFCLRCL PEPERQLSVP IPGGSNGDVQ QVGVIRCPVC
210 220 230 240 250
RQECRQIDLV DNYFVKDTSE APSSSDEKSE QVCTSCEDNA SAVGFCVECG
260 270 280 290 300
EWLCKTCIEA HQRVKFTKDH LIRKKEDVSE SVGTSGQRPV FCPVHKQEQL
310 320 330 340 350
KLFCETCDRL TCRDCQLLEH KEHRYQFLEE AFQNQKGAIE NLLAKLLEKK
360 370 380 390 400
NYVHFAATQV QNRIKEVNET NKRVEQEIKV AIFTLINEIN KKGKSLLQQL
410 420 430 440 450
ENVTKERQMK LLQQQNDITG LSRQVKHVMN FTNWAIASGS STALLYSKRL
460 470 480 490 500
ITFQLRHILK ARCDPVPAAN GAIRFHCDPT FWAKNVVNLG NLVIESKPAP
510 520 530 540 550
GYTPNVVVGQ VPPGTNHISK TPGQINLAQL RLQHMQQQVY AQKHQQLQQM
560 570 580 590 600
RLQQPPAPIP TTTATTQQHP RQAAPQMLQQ QPPRLISVQT MQRGNMNCGA
610 620 630 640 650
FQAHQMRLAQ NAARIPGIPR HSAPQYSMMQ PHLQRQHSNP GHAGPFPVVS
660 670 680 690 700
AHNPINPTSP TTATMANANR GPTSPSVTAI ELIPSVTNPE NLPSLPDIPP
710 720 730 740 750
IQLEDAGSSS LDNLLSRYIS GSHLPPQPTS TMNPSPGPSA LSPGSSGLSN
760 770 780 790 800
SHTPVRPPST SSTGSRGSCG SSGRTAEKSA HSFKSDQVKV KQEPGTEEEI
810 820 830 840 850
CSFSGAVKQE KTEDGRRSAC MLSSPESSLT PPLSTNLHLE SELDTLTGLE
860 870 880 890 900
NHVKTEPTDI SESCKQSGLS NLVNGKSPIR NLMHRSARIG GDGNSKDDDP
910 920 930 940 950
NEDWCAVCQN GGDLLCCEKC PKVFHLTCHV PTLLSFPSGD WICTFCRDIG
960 970 980 990 1000
KPEVEYDCDN MQHSKKGKTA QGLSPVDQRK CERLLLYLYC HELSIEFQEP
1010 1020 1030 1040 1050
VPVSIPNYYK IIKKPMDLST VKKKLQKKHS QHYQIPDDFV ADVRLIFKNC
1060 1070 1080 1090 1100
ERFNEMMKVV QVYADTQEIN LKGDSEVAKA GKAVALYFED KLSEIYSDRT
1110 1120 1130 1140
FTPLPEFEQD EDDGEVTEDS DEDFIQPRRK RLKSDERPVH IK
Length:1,142
Mass (Da):123,843
Last modified:December 7, 2004 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iD43699AE75F0893F
GO
Isoform Beta (identifier: Q99PP7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1056-1072: Missing.

Show »
Length:1,125
Mass (Da):121,850
Checksum:iE19A5D1F2973E83D
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9QP19E9QP19_MOUSE
E3 ubiquitin-protein ligase TRIM33
Trim33
1,140Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
E9QME5E9QME5_MOUSE
E3 ubiquitin-protein ligase TRIM33
Trim33
1,123Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
A0A0G2JGY5A0A0G2JGY5_MOUSE
E3 ubiquitin-protein ligase TRIM33
Trim33
193Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
A0A0G2JE09A0A0G2JE09_MOUSE
E3 ubiquitin-protein ligase TRIM33
Trim33
402Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
A0A0G2JFC3A0A0G2JFC3_MOUSE
E3 ubiquitin-protein ligase TRIM33
Trim33
291Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti949 – 952IGKP → HASA (PubMed:11331580).Curated4

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_0120681056 – 1072Missing in isoform Beta. 1 PublicationAdd BLAST17

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

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EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AY458590 mRNA Translation: AAS10352.1
AK129293 mRNA Translation: BAC98103.1
AF220138 mRNA Translation: AAG53511.1

NCBI Reference Sequences

More...
RefSeqi
NP_001073299.1, NM_001079830.2
NP_444400.2, NM_053170.3

Genome annotation databases

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
94093

KEGG: Kyoto Encyclopedia of Genes and Genomes

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KEGGi
mmu:94093

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY458590 mRNA Translation: AAS10352.1
AK129293 mRNA Translation: BAC98103.1
AF220138 mRNA Translation: AAG53511.1
RefSeqiNP_001073299.1, NM_001079830.2
NP_444400.2, NM_053170.3

3D structure databases

SMRiQ99PP7
ModBaseiSearch...

Protein-protein interaction databases

BioGRIDi220448, 5 interactors
CORUMiQ99PP7
DIPiDIP-59674N
IntActiQ99PP7, 6 interactors
MINTiQ99PP7
STRINGi10090.ENSMUSP00000029444

PTM databases

iPTMnetiQ99PP7
PhosphoSitePlusiQ99PP7

Proteomic databases

EPDiQ99PP7
jPOSTiQ99PP7
PaxDbiQ99PP7
PRIDEiQ99PP7

Genome annotation databases

GeneIDi94093
KEGGimmu:94093

Organism-specific databases

Comparative Toxicogenomics Database

More...
CTDi
51592
MGIiMGI:2137357, Trim33

Rodent Unidentified Gene-Encoded large proteins database

More...
Rougei
Search...

Phylogenomic databases

eggNOGiKOG2177, Eukaryota
InParanoidiQ99PP7
KOiK08883
OrthoDBi756911at2759
PhylomeDBiQ99PP7

Enzyme and pathway databases

UniPathwayiUPA00143
ReactomeiR-MMU-2173795, Downregulation of SMAD2/3:SMAD4 transcriptional activity

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...
BioGRID-ORCSi
94093, 5 hits in 22 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...
ChiTaRSi
Trim33, mouse

Protein Ontology

More...
PROi
PR:Q99PP7
RNActiQ99PP7, protein

The Stanford Online Universal Resource for Clones and ESTs

More...
SOURCEi
Search...

Family and domain databases

CDDicd00021, BBOX, 1 hit
Gene3Di1.20.920.10, 1 hit
3.30.40.10, 2 hits
InterProiView protein in InterPro
IPR003649, Bbox_C
IPR001487, Bromodomain
IPR036427, Bromodomain-like_sf
IPR019786, Zinc_finger_PHD-type_CS
IPR027370, Znf-RING_LisH
IPR000315, Znf_B-box
IPR011011, Znf_FYVE_PHD
IPR001965, Znf_PHD
IPR019787, Znf_PHD-finger
IPR001841, Znf_RING
IPR013083, Znf_RING/FYVE/PHD
IPR017907, Znf_RING_CS
PfamiView protein in Pfam
PF00439, Bromodomain, 1 hit
PF00628, PHD, 1 hit
PF00643, zf-B_box, 1 hit
PF13445, zf-RING_UBOX, 1 hit
PRINTSiPR00503, BROMODOMAIN
SMARTiView protein in SMART
SM00502, BBC, 1 hit
SM00336, BBOX, 2 hits
SM00297, BROMO, 1 hit
SM00249, PHD, 2 hits
SM00184, RING, 2 hits
SUPFAMiSSF47370, SSF47370, 1 hit
SSF57903, SSF57903, 1 hit
PROSITEiView protein in PROSITE
PS50014, BROMODOMAIN_2, 1 hit
PS50119, ZF_BBOX, 2 hits
PS01359, ZF_PHD_1, 1 hit
PS50016, ZF_PHD_2, 1 hit
PS00518, ZF_RING_1, 1 hit
PS50089, ZF_RING_2, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiTRI33_MOUSE
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q99PP7
Secondary accession number(s): Q6SI71, Q6ZPX5
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: December 7, 2004
Last modified: August 12, 2020
This is version 147 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families
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