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Protein

Leucine-rich repeat-containing protein 4

Gene

Lrrc4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Synaptic adhesion protein. Regulates the formation of exitatory synapses through the recruitment of pre-and-postsynaptic proteins. Organize the lamina/pathway-specific differentiation of dendrites. Plays a important role for auditory synaptic responses. Involved in the suppression of glioma.3 Publications

GO - Biological processi

  • modulation of chemical synaptic transmission Source: SynGO
  • postsynaptic density protein 95 clustering Source: MGI
  • regulation of synapse organization Source: MGI
  • synapse organization Source: MGI
  • synaptic membrane adhesion Source: MGI

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine-rich repeat-containing protein 4
Alternative name(s):
Brain tumor-associated protein MBAG1
Netrin-G2 ligand
Short name:
NGL-2
Gene namesi
Name:Lrrc4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:2182081 Lrrc4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini41 – 526ExtracellularSequence analysisAdd BLAST486
Transmembranei527 – 547HelicalSequence analysisAdd BLAST21
Topological domaini548 – 652CytoplasmicSequence analysisAdd BLAST105

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Disruption phenotypei

Mutant mice dysplay impaired startle response to acoustic stimulus.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 40Sequence analysisAdd BLAST40
ChainiPRO_000001483441 – 652Leucine-rich repeat-containing protein 4Add BLAST612

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi45 ↔ 51PROSITE-ProRule annotation
Disulfide bondi49 ↔ 60PROSITE-ProRule annotation
Glycosylationi276N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi303 ↔ 328PROSITE-ProRule annotation
Disulfide bondi305 ↔ 349PROSITE-ProRule annotation
Glycosylationi321N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi362N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi373 ↔ 423PROSITE-ProRule annotation
Glycosylationi387N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi409N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi433N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi439N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi449N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ99PH1
PRIDEiQ99PH1

PTM databases

PhosphoSitePlusiQ99PH1

Expressioni

Tissue specificityi

Specifically expressed in brain. In the hippocampus, parietal cortex and piriform cortex expressed in proximal segments of CA1 pyramidal neurons.2 Publications

Gene expression databases

BgeeiENSMUSG00000049939 Expressed in 181 organ(s), highest expression level in embryo
CleanExiMM_LRRC4
ExpressionAtlasiQ99PH1 baseline and differential
GenevisibleiQ99PH1 MM

Interactioni

Subunit structurei

Interacts (via LRR repeats) with NTNG2. Interacts with DLG4. Forms a complex with DLG4 and with NMDA receptors.2 Publications

Protein-protein interaction databases

DIPiDIP-46449N
IntActiQ99PH1, 1 interactor
STRINGi10090.ENSMUSP00000062158

Structurei

3D structure databases

ProteinModelPortaliQ99PH1
SMRiQ99PH1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini41 – 74LRRNTAdd BLAST34
Repeati75 – 96LRR 1Add BLAST22
Repeati99 – 120LRR 2Add BLAST22
Repeati123 – 144LRR 3Add BLAST22
Repeati147 – 168LRR 4Add BLAST22
Repeati171 – 193LRR 5Add BLAST23
Repeati196 – 217LRR 6Add BLAST22
Repeati218 – 239LRR 7Add BLAST22
Repeati242 – 263LRR 8Add BLAST22
Repeati266 – 287LRR 9Add BLAST22
Domaini299 – 351LRRCTAdd BLAST53
Domaini352 – 441Ig-likeAdd BLAST90

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi454 – 525Thr-richAdd BLAST72

Domaini

The last 4 C-terminal residues bind to the first 2 PDZ domains of DLG4.

Keywords - Domaini

Immunoglobulin domain, Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0619 Eukaryota
COG4886 LUCA
GeneTreeiENSGT00760000118831
HOGENOMiHOG000252924
HOVERGENiHBG052359
InParanoidiQ99PH1
KOiK16351
OMAiSWWLREY
OrthoDBiEOG091G037N
PhylomeDBiQ99PH1
TreeFamiTF324303

Family and domain databases

Gene3Di2.60.40.10, 1 hit
3.80.10.10, 1 hit
InterProiView protein in InterPro
IPR000483 Cys-rich_flank_reg_C
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR013098 Ig_I-set
IPR003599 Ig_sub
IPR003598 Ig_sub2
IPR001611 Leu-rich_rpt
IPR003591 Leu-rich_rpt_typical-subtyp
IPR032675 LRR_dom_sf
IPR026882 Lrrc4
IPR000372 LRRNT
PANTHERiPTHR24369:SF9 PTHR24369:SF9, 1 hit
PfamiView protein in Pfam
PF07679 I-set, 1 hit
PF13855 LRR_8, 3 hits
SMARTiView protein in SMART
SM00409 IG, 1 hit
SM00408 IGc2, 1 hit
SM00369 LRR_TYP, 7 hits
SM00082 LRRCT, 1 hit
SM00013 LRRNT, 1 hit
SUPFAMiSSF48726 SSF48726, 1 hit
PROSITEiView protein in PROSITE
PS50835 IG_LIKE, 1 hit
PS51450 LRR, 7 hits

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q99PH1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKLLWQVTVH HTWNAVLLPV VYLTAQVWIL CAAIAAAASA GPQNCPSVCS
60 70 80 90 100
CSNQFSKVVC TRRGLSEVPQ GIPSNTRYLN LMENNIQMIQ ADTFRHLHHL
110 120 130 140 150
EVLQLGRNSI RQIEVGAFNG LASLNTLELF DNWLTVIPSG AFEYLSKLRE
160 170 180 190 200
LWLRNNPIES IPSYAFNRVP SLMRLDLGEL KKLEYISEGA FEGLFNLKYL
210 220 230 240 250
NLGMCNIKDM PNLTPLVGLE ELEMSGNHFP EIRPGSFHGL SSLKKLWVMN
260 270 280 290 300
SQVSLIERNA FDGLASLVEL NLAHNNLSSL PHDLFTPLRY LVELHLHHNP
310 320 330 340 350
WNCDCDILWL AWWLREYIPT NSTCCGRCHA PMHMRGRYLV EVDQAAFQCS
360 370 380 390 400
APFIMDAPRD LNISEDRMAE LKCRTPPMSS VKWLLPNGTV LSHASRHPRI
410 420 430 440 450
SVLNDGTLNF SRVLLIDTGV YTCMVTNVAG NSNASAYLNV SSAELNTPNF
460 470 480 490 500
SFFTTVTVET TEISPEDITR KYKPVPTTST GYQPAYTTST TVLIQTTRVP
510 520 530 540 550
KQVPVPSTDT TDKMQTSLDE VMKTTKIIIG CFVAVTLLAA AMLIVFYKLR
560 570 580 590 600
KRHQQRSTVT AARTVEIIQV DEDIPAAAPA AATAAPSGVS GEGAVVLPTI
610 620 630 640 650
HDHINYNTYK PAHGAHWTEN SLGNSLHPTV TTISEPYIIQ THTKDKVQET

QI
Length:652
Mass (Da):72,619
Last modified:October 1, 2002 - v2
Checksum:i9D2807A1AEE30062
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9PUX8E9PUX8_MOUSE
Leucine-rich repeat-containing prot...
Lrrc4
177Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti252 – 256QVSLI → H in AAL67671 (Ref. 1) Curated5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF300458 mRNA Translation: AAL67671.1
DQ177325 mRNA Translation: AAG60620.2
BC117834 mRNA Translation: AAI17835.1
CCDSiCCDS39448.1
RefSeqiNP_619623.2, NM_138682.2
UniGeneiMm.443660

Genome annotation databases

EnsembliENSMUST00000062304; ENSMUSP00000062158; ENSMUSG00000049939
GeneIDi192198
KEGGimmu:192198
UCSCiuc009bcu.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF300458 mRNA Translation: AAL67671.1
DQ177325 mRNA Translation: AAG60620.2
BC117834 mRNA Translation: AAI17835.1
CCDSiCCDS39448.1
RefSeqiNP_619623.2, NM_138682.2
UniGeneiMm.443660

3D structure databases

ProteinModelPortaliQ99PH1
SMRiQ99PH1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-46449N
IntActiQ99PH1, 1 interactor
STRINGi10090.ENSMUSP00000062158

PTM databases

PhosphoSitePlusiQ99PH1

Proteomic databases

PaxDbiQ99PH1
PRIDEiQ99PH1

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000062304; ENSMUSP00000062158; ENSMUSG00000049939
GeneIDi192198
KEGGimmu:192198
UCSCiuc009bcu.1 mouse

Organism-specific databases

CTDi64101
MGIiMGI:2182081 Lrrc4

Phylogenomic databases

eggNOGiKOG0619 Eukaryota
COG4886 LUCA
GeneTreeiENSGT00760000118831
HOGENOMiHOG000252924
HOVERGENiHBG052359
InParanoidiQ99PH1
KOiK16351
OMAiSWWLREY
OrthoDBiEOG091G037N
PhylomeDBiQ99PH1
TreeFamiTF324303

Miscellaneous databases

PROiPR:Q99PH1
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000049939 Expressed in 181 organ(s), highest expression level in embryo
CleanExiMM_LRRC4
ExpressionAtlasiQ99PH1 baseline and differential
GenevisibleiQ99PH1 MM

Family and domain databases

Gene3Di2.60.40.10, 1 hit
3.80.10.10, 1 hit
InterProiView protein in InterPro
IPR000483 Cys-rich_flank_reg_C
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR013098 Ig_I-set
IPR003599 Ig_sub
IPR003598 Ig_sub2
IPR001611 Leu-rich_rpt
IPR003591 Leu-rich_rpt_typical-subtyp
IPR032675 LRR_dom_sf
IPR026882 Lrrc4
IPR000372 LRRNT
PANTHERiPTHR24369:SF9 PTHR24369:SF9, 1 hit
PfamiView protein in Pfam
PF07679 I-set, 1 hit
PF13855 LRR_8, 3 hits
SMARTiView protein in SMART
SM00409 IG, 1 hit
SM00408 IGc2, 1 hit
SM00369 LRR_TYP, 7 hits
SM00082 LRRCT, 1 hit
SM00013 LRRNT, 1 hit
SUPFAMiSSF48726 SSF48726, 1 hit
PROSITEiView protein in PROSITE
PS50835 IG_LIKE, 1 hit
PS51450 LRR, 7 hits
ProtoNetiSearch...

Entry informationi

Entry nameiLRRC4_MOUSE
AccessioniPrimary (citable) accession number: Q99PH1
Secondary accession number(s): Q149E5, Q8VI35
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: October 1, 2002
Last modified: November 7, 2018
This is version 136 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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