UniProtKB - Q99PG0 (AAAD_MOUSE)
Protein
Arylacetamide deacetylase
Gene
Aadac
Organism
Mus musculus (Mouse)
Status
Functioni
Displays cellular triglyceride lipase activity in liver, increases the levels of intracellular fatty acids derived from the hydrolysis of newly formed triglyceride stores and plays a role in very low-density lipoprotein assembly (By similarity). Displays serine esterase activity in liver. Deacetylates a variety of arylacetamide substrates, including xenobiotic compounds and procarcinogens, converting them to the primary arylamide compounds and increasing their toxicity.By similarity2 Publications
Catalytic activityi
- EC:3.1.1.31 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 188 | PROSITE-ProRule annotation | 1 | |
Active sitei | 342 | By similarity | 1 | |
Active sitei | 372 | By similarity | 1 |
GO - Molecular functioni
- deacetylase activity Source: UniProtKB
- hydrolase activity Source: GO_Central
- lipase activity Source: MGI
- serine hydrolase activity Source: UniProtKB
- triglyceride lipase activity Source: UniProtKB
GO - Biological processi
- positive regulation of triglyceride catabolic process Source: UniProtKB
Keywordsi
Molecular function | Hydrolase |
Enzyme and pathway databases
Reactomei | R-MMU-211945, Phase I - Functionalization of compounds |
SABIO-RKi | Q99PG0 |
Protein family/group databases
ESTHERi | mouse-aryla, Arylacetamide_deacetylase |
MEROPSi | S09.991 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Aadac Synonyms:Aada |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1915008, Aadac |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication; Single-pass type II membrane protein 1 Publication
- Microsome membrane By similarity; Single-pass type II membrane protein By similarity
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 5 | CytoplasmicSequence analysis | 5 | |
Transmembranei | 6 – 26 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 21 | |
Topological domaini | 27 – 398 | LumenalSequence analysisAdd BLAST | 372 |
Keywords - Cellular componenti
Endoplasmic reticulum, Membrane, MicrosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000071543 | 1 – 398 | Arylacetamide deacetylaseAdd BLAST | 398 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 115 ↔ 339 | By similarity | ||
Glycosylationi | 281 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
N-glycosylated.1 Publication
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
jPOSTi | Q99PG0 |
MaxQBi | Q99PG0 |
PaxDbi | Q99PG0 |
PeptideAtlasi | Q99PG0 |
PRIDEi | Q99PG0 |
PTM databases
GlyGeni | Q99PG0, 1 site |
iPTMneti | Q99PG0 |
PhosphoSitePlusi | Q99PG0 |
SwissPalmi | Q99PG0 |
Expressioni
Tissue specificityi
Highest levels in liver with lower levels in jejunum and kidney.2 Publications
Gene expression databases
Bgeei | ENSMUSG00000027761, Expressed in epithelium of small intestine and 141 other tissues |
Genevisiblei | Q99PG0, MM |
Interactioni
Protein-protein interaction databases
IntActi | Q99PG0, 3 interactors |
MINTi | Q99PG0 |
STRINGi | 10090.ENSMUSP00000029325 |
Miscellaneous databases
RNActi | Q99PG0, protein |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 110 – 112 | Involved in the stabilization of the negatively charged intermediate by the formation of the oxyanion holeBy similarity | 3 |
Sequence similaritiesi
Belongs to the 'GDXG' lipolytic enzyme family.Curated
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1515, Eukaryota |
GeneTreei | ENSGT00940000155975 |
HOGENOMi | CLU_012494_12_0_1 |
InParanoidi | Q99PG0 |
OMAi | MWINAHL |
OrthoDBi | 1263520at2759 |
PhylomeDBi | Q99PG0 |
TreeFami | TF314978 |
Family and domain databases
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR013094, AB_hydrolase_3 IPR017157, Arylacetamide_deacetylase IPR002168, Lipase_GDXG_HIS_AS IPR033140, Lipase_GDXG_put_SER_AS |
Pfami | View protein in Pfam PF07859, Abhydrolase_3, 2 hits |
PIRSFi | PIRSF037251, Arylacetamide_deacetylase, 1 hit |
SUPFAMi | SSF53474, SSF53474, 1 hit |
PROSITEi | View protein in PROSITE PS01173, LIPASE_GDXG_HIS, 1 hit PS01174, LIPASE_GDXG_SER, 1 hit |
i Sequence
Sequence statusi: Complete.
Q99PG0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGKTISLLIS VVLVAYYLYI PLPDAIEEPW KVVWETAFVK IGTDLASFGE
60 70 80 90 100
LLGISHFMET IQLLMSFQEV PPTSDEHVTV METAFDSVPV RIYIPKRKSM
110 120 130 140 150
ALRRGLFYIH GGGWCLGSAA HFSYDTLSRW TAHKLDAVVV STDYGLAPKH
160 170 180 190 200
HFPRQFEDVY RSLRWFLQED VLEKYGVDPR RVGVSGDSAG GNLAAAVTQQ
210 220 230 240 250
LIQDPDVKIK LKVQALIYPA LQALDTNVPS QQEGSHFPVL TRSLMVRFWS
260 270 280 290 300
EYFTTDRGLE KAMLLNQHVP MESSHLLQFV NWSSLLPERY KKSPVYKNPT
310 320 330 340 350
PGSSELAQKY PGFIDVKACP LLANDNILHH LPKTYIITCQ YDVLRDDGLM
360 370 380 390
YVKRLQNVGV HVTHHHVEDG FHGTFSFPGL KLSERMKNQY LSWLIKNL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 101 | A → T in AAH19999 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 101 | A → T in AAH54823 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF306788 Genomic DNA Translation: AAG60035.1 BC019999 mRNA Translation: AAH19999.1 BC054823 mRNA Translation: AAH54823.1 |
CCDSi | CCDS17375.1 |
RefSeqi | NP_075872.1, NM_023383.1 |
Genome annotation databases
Ensembli | ENSMUST00000029325; ENSMUSP00000029325; ENSMUSG00000027761 |
GeneIDi | 67758 |
KEGGi | mmu:67758 |
UCSCi | uc008piz.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF306788 Genomic DNA Translation: AAG60035.1 BC019999 mRNA Translation: AAH19999.1 BC054823 mRNA Translation: AAH54823.1 |
CCDSi | CCDS17375.1 |
RefSeqi | NP_075872.1, NM_023383.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
IntActi | Q99PG0, 3 interactors |
MINTi | Q99PG0 |
STRINGi | 10090.ENSMUSP00000029325 |
Chemistry databases
ChEMBLi | CHEMBL3509583 |
Protein family/group databases
ESTHERi | mouse-aryla, Arylacetamide_deacetylase |
MEROPSi | S09.991 |
PTM databases
GlyGeni | Q99PG0, 1 site |
iPTMneti | Q99PG0 |
PhosphoSitePlusi | Q99PG0 |
SwissPalmi | Q99PG0 |
Proteomic databases
jPOSTi | Q99PG0 |
MaxQBi | Q99PG0 |
PaxDbi | Q99PG0 |
PeptideAtlasi | Q99PG0 |
PRIDEi | Q99PG0 |
Protocols and materials databases
Antibodypediai | 1182, 193 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000029325; ENSMUSP00000029325; ENSMUSG00000027761 |
GeneIDi | 67758 |
KEGGi | mmu:67758 |
UCSCi | uc008piz.1, mouse |
Organism-specific databases
CTDi | 13 |
MGIi | MGI:1915008, Aadac |
Phylogenomic databases
eggNOGi | KOG1515, Eukaryota |
GeneTreei | ENSGT00940000155975 |
HOGENOMi | CLU_012494_12_0_1 |
InParanoidi | Q99PG0 |
OMAi | MWINAHL |
OrthoDBi | 1263520at2759 |
PhylomeDBi | Q99PG0 |
TreeFami | TF314978 |
Enzyme and pathway databases
Reactomei | R-MMU-211945, Phase I - Functionalization of compounds |
SABIO-RKi | Q99PG0 |
Miscellaneous databases
BioGRID-ORCSi | 67758, 0 hits in 17 CRISPR screens |
ChiTaRSi | Aadac, mouse |
PROi | PR:Q99PG0 |
RNActi | Q99PG0, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000027761, Expressed in epithelium of small intestine and 141 other tissues |
Genevisiblei | Q99PG0, MM |
Family and domain databases
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR013094, AB_hydrolase_3 IPR017157, Arylacetamide_deacetylase IPR002168, Lipase_GDXG_HIS_AS IPR033140, Lipase_GDXG_put_SER_AS |
Pfami | View protein in Pfam PF07859, Abhydrolase_3, 2 hits |
PIRSFi | PIRSF037251, Arylacetamide_deacetylase, 1 hit |
SUPFAMi | SSF53474, SSF53474, 1 hit |
PROSITEi | View protein in PROSITE PS01173, LIPASE_GDXG_HIS, 1 hit PS01174, LIPASE_GDXG_SER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AAAD_MOUSE | |
Accessioni | Q99PG0Primary (citable) accession number: Q99PG0 Secondary accession number(s): Q8VCF2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 4, 2005 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 140 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families