UniProtKB - Q99PE8 (ABCG5_MOUSE)
ATP-binding cassette sub-family G member 5
Abcg5
Functioni
ABCG5 and ABCG8 form an obligate heterodimer that mediates Mg2+- and ATP-dependent sterol transport across the cell membrane (PubMed:16352607, PubMed:16867993, PubMed:18402465).
Plays an essential role in the selective transport of dietary plant sterols and cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile (PubMed:12444248, PubMed:14504269, PubMed:14657202, PubMed:19846887, PubMed:25378657).
Required for normal sterol homeostasis (PubMed:12444248, PubMed:14657202).
The heterodimer with ABCG8 has ATPase activity (PubMed:16352607, PubMed:16867993).
8 PublicationsCatalytic activityi
- This reaction proceeds in the forward1 Publication direction.
- (24R)-ethylcholest-5-en-3β-ol(in) + ATP + H2O = (24R)-ethylcholest-5-en-3β-ol(out) + ADP + H+ + phosphate1 PublicationThis reaction proceeds in the forward1 Publication direction.
Cofactori
Activity regulationi
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 87 – 94 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ABC-type transporter activity Source: InterPro
- ATPase-coupled transmembrane transporter activity Source: MGI
- ATP binding Source: UniProtKB-KW
- ATP hydrolysis activity Source: Ensembl
- cholesterol transfer activity Source: Ensembl
- metal ion binding Source: UniProtKB-KW
- protein heterodimerization activity Source: UniProtKB
GO - Biological processi
- bile acid signaling pathway Source: Ensembl
- cholesterol efflux Source: BHF-UCL
- cholesterol homeostasis Source: MGI
- excretion Source: BHF-UCL
- intestinal cholesterol absorption Source: BHF-UCL
- negative regulation of intestinal cholesterol absorption Source: MGI
- negative regulation of intestinal phytosterol absorption Source: MGI
- response to ionizing radiation Source: Ensembl
- response to muscle activity Source: Ensembl
- response to nutrient Source: Ensembl
- response to xenobiotic stimulus Source: Ensembl
- sterol transport Source: UniProtKB
- triglyceride homeostasis Source: Ensembl
Keywordsi
Molecular function | Translocase |
Biological process | Lipid transport, Transport |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-1369062, ABC transporters in lipid homeostasis |
Names & Taxonomyi
Protein namesi | Recommended name: ATP-binding cassette sub-family G member 5Curated (EC:7.6.2.-1 Publication)Alternative name(s): Sterolin-12 Publications |
Gene namesi | Name:Abcg5Imported |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1351659, Abcg5 |
VEuPathDBi | HostDB:ENSMUSG00000040505 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication2 Publications; Multi-pass membrane protein Curated
- Apical cell membrane 5 Publications; Multi-pass membrane protein Curated
Plasma Membrane
- apical plasma membrane Source: UniProtKB
- ATP-binding cassette (ABC) transporter complex Source: MGI
- integral component of plasma membrane Source: UniProtKB
Other locations
- apical part of cell Source: MGI
- membrane Source: MGI
- receptor complex Source: BHF-UCL
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 384 | CytoplasmicBy similarityAdd BLAST | 384 | |
Transmembranei | 385 – 405 | Helical; Name=1By similarityAdd BLAST | 21 | |
Topological domaini | 406 – 422 | ExtracellularBy similarityAdd BLAST | 17 | |
Transmembranei | 423 – 443 | Helical; Name=2By similarityAdd BLAST | 21 | |
Topological domaini | 444 – 468 | CytoplasmicBy similarityAdd BLAST | 25 | |
Transmembranei | 469 – 490 | Helical; Name=3By similarityAdd BLAST | 22 | |
Topological domaini | 491 – 501 | ExtracellularBy similarityAdd BLAST | 11 | |
Transmembranei | 502 – 522 | Helical; Name=4By similarityAdd BLAST | 21 | |
Topological domaini | 523 – 529 | CytoplasmicBy similarity | 7 | |
Transmembranei | 530 – 550 | Helical; Name=5By similarityAdd BLAST | 21 | |
Topological domaini | 551 – 624 | ExtracellularBy similarityAdd BLAST | 74 | |
Transmembranei | 625 – 645 | Helical; Name=6By similarityAdd BLAST | 21 | |
Topological domaini | 646 – 652 | CytoplasmicBy similarity | 7 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Involvement in diseasei
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 61 | C → A: Abolishes palmitoylation. No effect on function and subcellular location. 1 Publication | 1 | |
Mutagenesisi | 93 | K → M: Disrupts sterol transport activity. Decreases expression of both ABCG5 and ABCG8. 2 Publications | 1 | |
Mutagenesisi | 93 | K → R: Strongly reduces cholesterol transport activity, but has little effect on biliary secretion of campesterol and sitosterol. No effect on ATP-binding and on expression of ABCG5 and ABCG8. 1 Publication | 1 | |
Mutagenesisi | 194 | I → V: No effect on cholesterol and sitosterol transport activity; when associated with G-196. 1 Publication | 1 | |
Mutagenesisi | 196 | S → G: No effect on cholesterol and sitosterol transport activity; when associated with V-194. 1 Publication | 1 | |
Mutagenesisi | 197 | G → D: No effect on cholesterol and sitosterol transport activity. Mildly reduced expression of ABCG5 and ABCG8. 1 Publication | 1 | |
Mutagenesisi | 219 | E → D: Decreases expression of both ABCG5 and ABCG8. Disrupts sterol transport activity. 1 Publication | 1 | |
Mutagenesisi | 219 | E → Q: Strongly decreases expression of both ABCG5 and ABCG8. Disrupts sterol transport activity. 1 Publication | 1 | |
Mutagenesisi | 585 | N → Q: Loss of one N-glycosylation site. Abolishes N-glycosylation; when associated with Q-592. 1 Publication | 1 | |
Mutagenesisi | 592 | N → Q: Loss of one N-glycosylation site. Abolishes N-glycosylation; when associated with Q-585. 1 Publication | 1 |
Keywords - Diseasei
Disease variantPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000093394 | 1 – 652 | ATP-binding cassette sub-family G member 5Add BLAST | 652 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 61 | S-palmitoyl cysteine1 Publication | 1 | |
Glycosylationi | 410 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 585 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 592 | N-linked (GlcNAc...) asparagine1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Glycoprotein, Lipoprotein, PalmitateProteomic databases
jPOSTi | Q99PE8 |
MaxQBi | Q99PE8 |
PaxDbi | Q99PE8 |
PRIDEi | Q99PE8 |
ProteomicsDBi | 285820 |
PTM databases
GlyGeni | Q99PE8, 3 sites |
iPTMneti | Q99PE8 |
PhosphoSitePlusi | Q99PE8 |
SwissPalmi | Q99PE8 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000040505, Expressed in jejunum and 115 other tissues |
ExpressionAtlasi | Q99PE8, baseline and differential |
Genevisiblei | Q99PE8, MM |
Interactioni
Subunit structurei
Heterodimer with ABCG8.
6 PublicationsGO - Molecular functioni
- protein heterodimerization activity Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 205215, 1 interactor |
CORUMi | Q99PE8 |
STRINGi | 10090.ENSMUSP00000069495 |
Miscellaneous databases
RNActi | Q99PE8, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 39 – 294 | ABC transporterPROSITE-ProRule annotationAdd BLAST | 256 | |
Domaini | 389 – 646 | ABC transmembrane type-2Add BLAST | 258 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 25 | DisorderedSequence analysisAdd BLAST | 25 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0061, Eukaryota |
GeneTreei | ENSGT00940000157985 |
HOGENOMi | CLU_000604_57_9_1 |
InParanoidi | Q99PE8 |
OMAi | DCGYPCP |
OrthoDBi | 1022017at2759 |
PhylomeDBi | Q99PE8 |
TreeFami | TF105212 |
Family and domain databases
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR013525, ABC_2_trans IPR003439, ABC_transporter-like_ATP-bd IPR017871, ABC_transporter-like_CS IPR043926, ABCG_dom IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF01061, ABC2_membrane, 1 hit PF19055, ABC2_membrane_7, 1 hit PF00005, ABC_tran, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00211, ABC_TRANSPORTER_1, 1 hit PS50893, ABC_TRANSPORTER_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MGELPFLSPE GARGPHINRG SLSSLEQGSV TGTEARHSLG VLHVSYSVSN
60 70 80 90 100
RVGPWWNIKS CQQKWDRQIL KDVSLYIESG QIMCILGSSG SGKTTLLDAI
110 120 130 140 150
SGRLRRTGTL EGEVFVNGCE LRRDQFQDCF SYVLQSDVFL SSLTVRETLR
160 170 180 190 200
YTAMLALCRS SADFYNKKVE AVMTELSLSH VADQMIGSYN FGGISSGERR
210 220 230 240 250
RVSIAAQLLQ DPKVMMLDEP TTGLDCMTAN QIVLLLAELA RRDRIVIVTI
260 270 280 290 300
HQPRSELFQH FDKIAILTYG ELVFCGTPEE MLGFFNNCGY PCPEHSNPFD
310 320 330 340 350
FYMDLTSVDT QSREREIETY KRVQMLECAF KESDIYHKIL ENIERARYLK
360 370 380 390 400
TLPTVPFKTK DPPGMFGKLG VLLRRVTRNL MRNKQAVIMR LVQNLIMGLF
410 420 430 440 450
LIFYLLRVQN NTLKGAVQDR VGLLYQLVGA TPYTGMLNAV NLFPMLRAVS
460 470 480 490 500
DQESQDGLYH KWQMLLAYVL HVLPFSVIAT VIFSSVCYWT LGLYPEVARF
510 520 530 540 550
GYFSAALLAP HLIGEFLTLV LLGIVQNPNI VNSIVALLSI SGLLIGSGFI
560 570 580 590 600
RNIQEMPIPL KILGYFTFQK YCCEILVVNE FYGLNFTCGG SNTSMLNHPM
610 620 630 640 650
CAITQGVQFI EKTCPGATSR FTANFLILYG FIPALVILGI VIFKVRDYLI
SR
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketE9QA91 | E9QA91_MOUSE | ATP-binding cassette sub-family G m... | Abcg5 | 480 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 462 – 652 | Missing in trac; strongly increased levels of sitosterol, brassicasterol and campesterol in blood plasma. 1 PublicationAdd BLAST | 191 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF312713 mRNA Translation: AAG53097.1 AH011511 , AF351786, AF351787, AF351789, AF351790, AF351791, AF351792, AF351793, AF351794, AF351795, AF351796, AF351797 Genomic DNA Translation: AAL82586.1 AY195873 mRNA Translation: AAO45094.1 AK149569 mRNA Translation: BAE28965.1 CH466537 Genomic DNA Translation: EDL38580.1 BC106766 mRNA Translation: AAI06767.1 |
CCDSi | CCDS29001.1 |
RefSeqi | NP_114090.1, NM_031884.2 |
Genome annotation databases
Ensembli | ENSMUST00000066175; ENSMUSP00000069495; ENSMUSG00000040505 |
GeneIDi | 27409 |
KEGGi | mmu:27409 |
UCSCi | uc008dsz.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF312713 mRNA Translation: AAG53097.1 AH011511 , AF351786, AF351787, AF351789, AF351790, AF351791, AF351792, AF351793, AF351794, AF351795, AF351796, AF351797 Genomic DNA Translation: AAL82586.1 AY195873 mRNA Translation: AAO45094.1 AK149569 mRNA Translation: BAE28965.1 CH466537 Genomic DNA Translation: EDL38580.1 BC106766 mRNA Translation: AAI06767.1 |
CCDSi | CCDS29001.1 |
RefSeqi | NP_114090.1, NM_031884.2 |
3D structure databases
SMRi | Q99PE8 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 205215, 1 interactor |
CORUMi | Q99PE8 |
STRINGi | 10090.ENSMUSP00000069495 |
PTM databases
GlyGeni | Q99PE8, 3 sites |
iPTMneti | Q99PE8 |
PhosphoSitePlusi | Q99PE8 |
SwissPalmi | Q99PE8 |
Proteomic databases
jPOSTi | Q99PE8 |
MaxQBi | Q99PE8 |
PaxDbi | Q99PE8 |
PRIDEi | Q99PE8 |
ProteomicsDBi | 285820 |
Protocols and materials databases
Antibodypediai | 14899, 234 antibodies from 31 providers |
DNASUi | 27409 |
Genome annotation databases
Ensembli | ENSMUST00000066175; ENSMUSP00000069495; ENSMUSG00000040505 |
GeneIDi | 27409 |
KEGGi | mmu:27409 |
UCSCi | uc008dsz.1, mouse |
Organism-specific databases
CTDi | 64240 |
MGIi | MGI:1351659, Abcg5 |
VEuPathDBi | HostDB:ENSMUSG00000040505 |
Phylogenomic databases
eggNOGi | KOG0061, Eukaryota |
GeneTreei | ENSGT00940000157985 |
HOGENOMi | CLU_000604_57_9_1 |
InParanoidi | Q99PE8 |
OMAi | DCGYPCP |
OrthoDBi | 1022017at2759 |
PhylomeDBi | Q99PE8 |
TreeFami | TF105212 |
Enzyme and pathway databases
Reactomei | R-MMU-1369062, ABC transporters in lipid homeostasis |
Miscellaneous databases
BioGRID-ORCSi | 27409, 0 hits in 66 CRISPR screens |
ChiTaRSi | Abcg5, mouse |
PROi | PR:Q99PE8 |
RNActi | Q99PE8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000040505, Expressed in jejunum and 115 other tissues |
ExpressionAtlasi | Q99PE8, baseline and differential |
Genevisiblei | Q99PE8, MM |
Family and domain databases
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR013525, ABC_2_trans IPR003439, ABC_transporter-like_ATP-bd IPR017871, ABC_transporter-like_CS IPR043926, ABCG_dom IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF01061, ABC2_membrane, 1 hit PF19055, ABC2_membrane_7, 1 hit PF00005, ABC_tran, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00211, ABC_TRANSPORTER_1, 1 hit PS50893, ABC_TRANSPORTER_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ABCG5_MOUSE | |
Accessioni | Q99PE8Primary (citable) accession number: Q99PE8 Secondary accession number(s): Q540E8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 5, 2001 |
Last sequence update: | June 1, 2001 | |
Last modified: | February 23, 2022 | |
This is version 168 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families