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Protein

Contactin-associated protein-like 4

Gene

Cntnap4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Presynaptic protein involved in both dopaminergic synaptic transmission and GABAergic system, thereby participating in the structural maturation of inhibitory interneuron synapses. Involved in the dopaminergic synaptic transmission by attenuating dopamine release through a presynaptic mechanism. Also participates in the GABAergic system.1 Publication

GO - Biological processi

  • cell adhesion Source: UniProtKB-KW
  • regulation of grooming behavior Source: UniProtKB
  • regulation of synapse organization Source: SynGO
  • regulation of synaptic transmission, dopaminergic Source: UniProtKB
  • regulation of synaptic transmission, GABAergic Source: UniProtKB

Keywordsi

Biological processCell adhesion

Names & Taxonomyi

Protein namesi
Recommended name:
Contactin-associated protein-like 4
Alternative name(s):
Cell recognition molecule Caspr4
Gene namesi
Name:Cntnap4
Synonyms:Caspr4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:2183572 Cntnap4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini28 – 1243ExtracellularSequence analysisAdd BLAST1216
Transmembranei1244 – 1264HelicalSequence analysisAdd BLAST21
Topological domaini1265 – 1310CytoplasmicSequence analysisAdd BLAST46

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Synapse

Pathology & Biotechi

Disruption phenotypei

Synaptic defects characterized by increased dopamine but decreased GABA signaling. A reduction in the output of cortical parvalbumin (PV)-positive GABAergic basket cells is observed, together with an increase of midbrain dopaminergic release in the nucleus accumbens. Increased dopaminergic signaling induces behavior abnormalities, characterized by severe and highly penetrant over-grooming behavior, resulting in whisker, face and sometimes body hair loss but rarely lesions. The over-grooming phenotype can be pharmacologically reversed following administration of haloperidol drug.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
ChainiPRO_000001951128 – 1310Contactin-associated protein-like 4Add BLAST1283

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi33 ↔ 179By similarity
Glycosylationi262N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi287N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi334 ↔ 366By similarity
Glycosylationi361N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi517 ↔ 549By similarity
Glycosylationi540N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi555 ↔ 566By similarity
Disulfide bondi560 ↔ 575By similarity
Glycosylationi576N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi577 ↔ 587By similarity
Glycosylationi604N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi627N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi639N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi708N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi750N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi933 ↔ 960By similarity
Disulfide bondi964 ↔ 977By similarity
Disulfide bondi971 ↔ 986By similarity
Disulfide bondi988 ↔ 998By similarity
Glycosylationi1019N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1025N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1075N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi1169 ↔ 1204By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ99P47
PaxDbiQ99P47
PRIDEiQ99P47

PTM databases

iPTMnetiQ99P47
PhosphoSitePlusiQ99P47

Expressioni

Tissue specificityi

Specifically present in developing cortical interneurons: highly expressed in cortical parvalbumin (PV) cells and midbrain dopaminergic neurons and is localized presynaptically (at protein level). Also present in the substantia nigra pars compacta (SnC) and ventral tegmental area (VTA) midbrain dopaminergic projection populations.1 Publication

Gene expression databases

BgeeiENSMUSG00000031772 Expressed in 102 organ(s), highest expression level in substantia nigra
CleanExiMM_CNTNAP4
ExpressionAtlasiQ99P47 baseline and differential
GenevisibleiQ99P47 MM

Interactioni

Subunit structurei

Interacts with TIAM1.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034225

Structurei

3D structure databases

ProteinModelPortaliQ99P47
SMRiQ99P47
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 179F5/8 type CPROSITE-ProRule annotationAdd BLAST147
Domaini214 – 346Laminin G-like 1PROSITE-ProRule annotationAdd BLAST133
Domaini400 – 529Laminin G-like 2PROSITE-ProRule annotationAdd BLAST130
Domaini551 – 588EGF-like 1PROSITE-ProRule annotationAdd BLAST38
Domaini589 – 794Fibrinogen C-terminalPROSITE-ProRule annotationAdd BLAST206
Domaini795 – 960Laminin G-like 3PROSITE-ProRule annotationAdd BLAST166
Domaini960 – 999EGF-like 2PROSITE-ProRule annotationAdd BLAST40
Domaini1048 – 1204Laminin G-like 4PROSITE-ProRule annotationAdd BLAST157

Sequence similaritiesi

Belongs to the neurexin family.Curated

Keywords - Domaini

EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3516 Eukaryota
ENOG410XPHG LUCA
GeneTreeiENSGT00760000118991
HOVERGENiHBG057718
InParanoidiQ99P47
OrthoDBiEOG091G00LF

Family and domain databases

CDDicd00057 FA58C, 1 hit
Gene3Di2.60.120.260, 1 hit
InterProiView protein in InterPro
IPR028875 CASPR4
IPR013320 ConA-like_dom_sf
IPR000742 EGF-like_dom
IPR000421 FA58C
IPR036056 Fibrinogen-like_C
IPR002181 Fibrinogen_a/b/g_C_dom
IPR008979 Galactose-bd-like_sf
IPR001791 Laminin_G
PANTHERiPTHR43925:SF2 PTHR43925:SF2, 1 hit
PfamiView protein in Pfam
PF00754 F5_F8_type_C, 1 hit
PF02210 Laminin_G_2, 4 hits
SMARTiView protein in SMART
SM00181 EGF, 2 hits
SM00231 FA58C, 1 hit
SM00282 LamG, 4 hits
SUPFAMiSSF49785 SSF49785, 1 hit
SSF49899 SSF49899, 4 hits
SSF56496 SSF56496, 1 hit
PROSITEiView protein in PROSITE
PS50026 EGF_3, 2 hits
PS01285 FA58C_1, 1 hit
PS01286 FA58C_2, 1 hit
PS50022 FA58C_3, 1 hit
PS51406 FIBRINOGEN_C_2, 1 hit
PS50025 LAM_G_DOMAIN, 4 hits

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q99P47-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNMGSVAGAV LKMLLLLSTQ NWNRVEAGNS YDCDEPLVSA LPQASFSSSS
60 70 80 90 100
ELSSSHGPGF ARLNRRDGAG GWSPLVSNKY QWLQIDLGER MEVTSVATQG
110 120 130 140 150
GYGSSNWVTS YLLMFSDSGR NWKQYRQEDS IWGFSGNANA DSVVYYRLQP
160 170 180 190 200
SIKARFLRFI PLEWNPKGRI GMRIEVFGCA YRSVVIDLDG KSSLLYRFDQ
210 220 230 240 250
NSLSPIRDII SLKFKTMESD GILLHRAGPA GDHITLELRR GKLFLLINSG
260 270 280 290 300
DARLTSSSTL INLTLGSLLD DQHWHSVLIQ RLGKQVNFTV DEHRRHFHAQ
310 320 330 340 350
GEFNYLDLDY EISFGGISAP AKSVSLPYKH FHGCLENLFY NGVDVIGLVK
360 370 380 390 400
EHSPQIITMG NASFSCSQPQ SMPLTFLSPR SYLVLPASTK EEAISASFQF
410 420 430 440 450
RTWNKAGLLL FSELQLVSGS LLLLLSDGKL KLTLYQPGKS PSDITAGAGL
460 470 480 490 500
GDGQWHSVSL SAKRNHLSVV VDGHISPASP WLGPEQVNSG GVFYFGGCPD
510 520 530 540 550
KGFGSKCKSP LGGFQGCMRL ISINNKMVDL IAVQQGALGN FSDLQIDSCG
560 570 580 590 600
ISDRCLPNSC EHGGECSQSW STFHCNCTNT GYTGATCHSS VYEQSCEAYK
610 620 630 640 650
HQGNASGFYY IDSDGSGPLQ PFLLYCNMTE TAWTVMQHNG SDLMRVRNTH
660 670 680 690 700
SENAHTGVFE YTASMEQLQA AINRAEHCQQ ELVYYCKKSR LVNQQDGSPR
710 720 730 740 750
SWWVGRTNET QTYWGGSLPV HQKCTCGLEG NCIDAQYHCN CDADLNEWTN
760 770 780 790 800
DTGFLSYKEH LPVTKIVITD TGRPHSEAAY KLGPLLCRGD RPFWNAASFN
810 820 830 840 850
TEASYLHFPT FHGELSADVS FFFKTTALSG VFLENLGITD FIRIELRSPT
860 870 880 890 900
TVTFSFDVGN GPFELSVHSP THFNDNQWHH VRVERNMKEA SLRVDELPPK
910 920 930 940 950
IQAAPTDGHV LLQLNSQLFV GGTATRQRGF LGCIRSLQLN GMALDLEERA
960 970 980 990 1000
TVTPGVQPGC RGHCGSYGKL CRHGGKCREK PSGFFCDCSS SAYAGPFCSK
1010 1020 1030 1040 1050
EISAYFGSGS SVIYNFQENY SLSKNSSFHA ASFHGDMKLS REMIKFSFRT
1060 1070 1080 1090 1100
TRAPSLLLHM SSFYKEYLSI IIAKNGSLQI RYKLNKYHEP DVISFDLKSM
1110 1120 1130 1140 1150
ADGQLHHIKI NREEGMVFVE IDENTRRQTY LSSGTEFSAV KSLVLGRMLE
1160 1170 1180 1190 1200
YSDVDQETAL AAAHGFTGCL SAVQFSHIAP LKAALQPGPP APVTVTGHVT
1210 1220 1230 1240 1250
ESSCVAPSGT DATSRERTHS FADHSGTMDD REPLTHAIKS DSAVIGGLIA
1260 1270 1280 1290 1300
VVIFILLCVS AIAVRIYQQK RLYKRNEAKR SENVDSAEAV LKSELHIQNA
1310
VGENQKEYFF
Length:1,310
Mass (Da):144,730
Last modified:July 27, 2011 - v2
Checksum:iBEC6AB0A11DE6A14
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3YWB9D3YWB9_MOUSE
Contactin-associated protein-like 4
Cntnap4
1,310Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti993Y → F in AAG52890 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF333770 mRNA Translation: AAG52890.1
AK142816 mRNA Translation: BAE25200.1
CH466525 Genomic DNA Translation: EDL11532.1
BC034628 mRNA Translation: AAH34628.1
CCDSiCCDS40482.1
RefSeqiNP_569724.2, NM_130457.2
UniGeneiMm.209232
Mm.403088

Genome annotation databases

EnsembliENSMUST00000034225; ENSMUSP00000034225; ENSMUSG00000031772
GeneIDi170571
KEGGimmu:170571
UCSCiuc009nnj.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF333770 mRNA Translation: AAG52890.1
AK142816 mRNA Translation: BAE25200.1
CH466525 Genomic DNA Translation: EDL11532.1
BC034628 mRNA Translation: AAH34628.1
CCDSiCCDS40482.1
RefSeqiNP_569724.2, NM_130457.2
UniGeneiMm.209232
Mm.403088

3D structure databases

ProteinModelPortaliQ99P47
SMRiQ99P47
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034225

PTM databases

iPTMnetiQ99P47
PhosphoSitePlusiQ99P47

Proteomic databases

MaxQBiQ99P47
PaxDbiQ99P47
PRIDEiQ99P47

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034225; ENSMUSP00000034225; ENSMUSG00000031772
GeneIDi170571
KEGGimmu:170571
UCSCiuc009nnj.1 mouse

Organism-specific databases

CTDi85445
MGIiMGI:2183572 Cntnap4

Phylogenomic databases

eggNOGiKOG3516 Eukaryota
ENOG410XPHG LUCA
GeneTreeiENSGT00760000118991
HOVERGENiHBG057718
InParanoidiQ99P47
OrthoDBiEOG091G00LF

Miscellaneous databases

PROiPR:Q99P47
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031772 Expressed in 102 organ(s), highest expression level in substantia nigra
CleanExiMM_CNTNAP4
ExpressionAtlasiQ99P47 baseline and differential
GenevisibleiQ99P47 MM

Family and domain databases

CDDicd00057 FA58C, 1 hit
Gene3Di2.60.120.260, 1 hit
InterProiView protein in InterPro
IPR028875 CASPR4
IPR013320 ConA-like_dom_sf
IPR000742 EGF-like_dom
IPR000421 FA58C
IPR036056 Fibrinogen-like_C
IPR002181 Fibrinogen_a/b/g_C_dom
IPR008979 Galactose-bd-like_sf
IPR001791 Laminin_G
PANTHERiPTHR43925:SF2 PTHR43925:SF2, 1 hit
PfamiView protein in Pfam
PF00754 F5_F8_type_C, 1 hit
PF02210 Laminin_G_2, 4 hits
SMARTiView protein in SMART
SM00181 EGF, 2 hits
SM00231 FA58C, 1 hit
SM00282 LamG, 4 hits
SUPFAMiSSF49785 SSF49785, 1 hit
SSF49899 SSF49899, 4 hits
SSF56496 SSF56496, 1 hit
PROSITEiView protein in PROSITE
PS50026 EGF_3, 2 hits
PS01285 FA58C_1, 1 hit
PS01286 FA58C_2, 1 hit
PS50022 FA58C_3, 1 hit
PS51406 FIBRINOGEN_C_2, 1 hit
PS50025 LAM_G_DOMAIN, 4 hits
ProtoNetiSearch...

Entry informationi

Entry nameiCNTP4_MOUSE
AccessioniPrimary (citable) accession number: Q99P47
Secondary accession number(s): Q8K002
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 5, 2001
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 133 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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