UniProtKB - Q99P30 (NUDT7_MOUSE)
Protein
Peroxisomal coenzyme A diphosphatase NUDT7
Gene
Nudt7
Organism
Mus musculus (Mouse)
Status
Functioni
Coenzyme A diphosphatase which mediates the cleavage of CoA, CoA esters and oxidized CoA with similar efficiencies, yielding 3',5'-ADP and the corresponding 4'-phosphopantetheine derivative as products. Preferentially hydrolyzes medium-chain acyl-CoAs and bile acid-CoAs (PubMed:18799520). CoA into 3',5'-ADP and 4'-phosphopantetheine. Has no activity toward NDP-sugars, CDP-alcohols, (deoxy)nucleoside 5'-triphosphates, nucleoside 5'-di or monophosphates, diadenosine polyphosphates, NAD, NADH, NADP, NADPH or thymidine-5'-monophospho-p-nitrophenyl ester. May be required to eliminate oxidized CoA from peroxisomes, or regulate CoA and acyl-CoA levels in this organelle in response to metabolic demand. Does not play a role in U8 snoRNA decapping activity. Binds U8 snoRNA.2 Publications
Catalytic activityi
- H2O + hexanoyl-CoA = adenosine 3',5'-bisphosphate + 2 H+ + hexanoyl-4ʼ-phosphopantetheine1 PublicationThis reaction proceeds in the forward1 Publication direction.
- H2O + octanoyl-CoA = adenosine 3',5'-bisphosphate + 2 H+ + octanoyl-4ʼ-phosphopantetheine1 PublicationThis reaction proceeds in the forward1 Publication direction.
- butanoyl-CoA + H2O = adenosine 3',5'-bisphosphate + butanoyl-4ʼ-phosphopantetheine + 2 H+1 PublicationThis reaction proceeds in the forward1 Publication direction.
- decanoyl-CoA + H2O = adenosine 3',5'-bisphosphate + decanoyl-4ʼ-phosphopantetheine + 2 H+1 PublicationThis reaction proceeds in the forward1 Publication direction.
- dodecanoyl-CoA + H2O = adenosine 3',5'-bisphosphate + dodecanoyl-4ʼ-phosphopantetheine + 2 H+1 PublicationThis reaction proceeds in the forward1 Publication direction.
- H2O + tetradecanoyl-CoA = adenosine 3',5'-bisphosphate + 2 H+ + tetradecanoyl-4ʼ-phosphopantetheine1 PublicationThis reaction proceeds in the forward1 Publication direction.
- choloyl-CoA + H2O = adenosine 3',5'-bisphosphate + choloyl-4ʼ-phosphopantetheine + 2 H+1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 3α,7α,12α-trihydroxy-5β-cholestan-26-oyl-CoA + H2O = 3α,7α,12α-trihydroxy-5β-cholestan-26-oyl-4ʼ-phosphopantetheine + adenosine 3',5'-bisphosphate + 2 H+1 PublicationThis reaction proceeds in the forward1 Publication direction.
- acetyl-CoA + H2O = adenosine 3',5'-bisphosphate + 2 H+ + S-acetyl-4'-phosphopantetheine1 PublicationThis reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
Cofactori
Activity regulationi
Inhibited by fluoride.
Kineticsi
- KM=0.240 mM for CoA1 Publication
- KM=0.157 mM for CoA1 Publication
- KM=0.270 mM for acetyl-CoA1 Publication
- KM=92.6 µM for hexanoyl-CoA1 Publication
- KM=62.6 µM for octanoyl-CoA1 Publication
- KM=34 µM for tetradecanoyl-CoA1 Publication
- KM=157.9 µM for butanoyl-CoA1 Publication
- KM=242 µM for decanoyl-CoA1 Publication
- KM=22.4 µM for dodecanoyl-CoA1 Publication
- KM=221.4 µM for propionyl-CoA1 Publication
- KM=28.7 µM for palmitoyl-CoA1 Publication
- KM=0.430 mM for acetyl-CoA1 Publication
- KM=0.235 mM for CoA-S-S-CoA1 Publication
- KM=0.330 mM for succinyl-CoA1 Publication
- KM=0.480 mM for 3'-dephospho-CoA1 Publication
- Vmax=0.13 µmol/min/mg enzyme toward CoA1 Publication
- Vmax=0.27 µmol/min/mg enzyme toward acetyl-CoA1 Publication
- Vmax=0.33 µmol/min/mg enzyme toward propionyl-CoA1 Publication
- Vmax=0.49 µmol/min/mg enzyme toward butanoyl-CoA1 Publication
- Vmax=0.83 µmol/min/mg enzyme toward hexanoyl-CoA1 Publication
- Vmax=1.08 µmol/min/mg enzyme toward octanoyl-CoA1 Publication
- Vmax=1.84 µmol/min/mg enzyme toward decanoyl-CoA1 Publication
- Vmax=1.06 µmol/min/mg enzyme toward dodecanoyl-CoA1 Publication
- Vmax=0.41 µmol/min/mg enzyme toward tetradecanoyl-CoA1 Publication
- Vmax=0.15 µmol/min/mg enzyme toward palmitoyl-CoA1 Publication
pH dependencei
Optimum pH is 8.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 92 | Magnesium or manganeseBy similarity | 1 | |
Metal bindingi | 96 | Magnesium or manganeseBy similarity | 1 |
GO - Molecular functioni
- acetyl-CoA hydrolase activity Source: MGI
- hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides Source: InterPro
- magnesium ion binding Source: InterPro
- manganese ion binding Source: InterPro
- snoRNA binding Source: UniProtKB
GO - Biological processi
- acetyl-CoA catabolic process Source: UniProtKB
- brown fat cell differentiation Source: MGI
- butyryl-CoA catabolic process Source: UniProtKB
- coenzyme A catabolic process Source: UniProtKB
- medium-chain fatty-acyl-CoA catabolic process Source: UniProtKB
- nucleoside diphosphate metabolic process Source: InterPro
- propionyl-CoA catabolic process Source: UniProtKB
Keywordsi
Molecular function | Hydrolase, RNA-binding |
Ligand | Magnesium, Manganese, Metal-binding |
Enzyme and pathway databases
Reactomei | R-MMU-9033241, Peroxisomal protein import |
Chemistry databases
SwissLipidsi | SLP:000001595 [Q99P30-1] |
Names & Taxonomyi
Protein namesi | Recommended name: Peroxisomal coenzyme A diphosphatase NUDT7 (EC:3.6.1.-)Alternative name(s): Nucleoside diphosphate-linked moiety X motif 7 Short name: Nudix motif 7 |
Gene namesi | Name:Nudt7Imported |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1914778, Nudt7 |
Subcellular locationi
Peroxisome
- Peroxisome 2 Publications
Peroxisome
- peroxisome Source: UniProtKB
Keywords - Cellular componenti
PeroxisomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000057141 | 1 – 236 | Peroxisomal coenzyme A diphosphatase NUDT7Add BLAST | 236 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 20 | N6-succinyllysineCombined sources | 1 | |
Modified residuei | 178 | N6-succinyllysineCombined sources | 1 |
Proteomic databases
jPOSTi | Q99P30 |
PaxDbi | Q99P30 |
PeptideAtlasi | Q99P30 |
PRIDEi | Q99P30 |
2D gel databases
REPRODUCTION-2DPAGEi | Q99P30 |
PTM databases
iPTMneti | Q99P30 |
PhosphoSitePlusi | Q99P30 |
SwissPalmi | Q99P30 |
Expressioni
Tissue specificityi
Highly expressed in liver, brown adipose tissue and heart. Expressed at intermediate level in lung and kidney and at low level in brain.2 Publications
Expressed in liver, brown adipose tissue and heart at 20 times lower levels than isoform 1.1 Publication
Inductioni
Expression decreases in response to peroxisome proliferators.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000031767, Expressed in liver and 279 other tissues |
Genevisiblei | Q99P30, MM |
Interactioni
Protein-protein interaction databases
IntActi | Q99P30, 2 interactors |
MINTi | Q99P30 |
STRINGi | 10090.ENSMUSP00000073213 |
Miscellaneous databases
RNActi | Q99P30, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 37 – 169 | Nudix hydrolasePROSITE-ProRule annotationAdd BLAST | 133 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 77 – 98 | Nudix boxAdd BLAST | 22 | |
Motifi | 234 – 236 | Microbody targeting signalCurated | 3 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3069, Eukaryota |
GeneTreei | ENSGT00940000159631 |
HOGENOMi | CLU_040940_6_0_1 |
InParanoidi | Q99P30 |
OMAi | CFEYTDP |
OrthoDBi | 1253012at2759 |
PhylomeDBi | Q99P30 |
TreeFami | TF106350 |
Family and domain databases
InterProi | View protein in InterPro IPR015797, NUDIX_hydrolase-like_dom_sf IPR000086, NUDIX_hydrolase_dom IPR000059, NUDIX_hydrolase_NudL_CS |
Pfami | View protein in Pfam PF00293, NUDIX, 1 hit |
SUPFAMi | SSF55811, SSF55811, 1 hit |
PROSITEi | View protein in PROSITE PS51462, NUDIX, 1 hit PS01293, NUDIX_COA, 1 hit |
s (5)i Sequence
Sequence statusi: Complete.
This entry describes 5 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q99P30-1) [UniParc]FASTAAdd to basket
Also known as: Alpha, Nudt7alpha1 Publication
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSRPCGLPEP VRNNLIDDAK ARLRKSDVGT RYSHLSSNKF SVLVPLLARG
60 70 80 90 100
GKLYLMFTVR SDKLKREPGE VCFPGGKRDP VDTDDTATAL REAQEEVGLH
110 120 130 140 150
PHQVEVVSHL VPYVFDNDAL VTPVVGFLDH NFQAQPNADE VKEVFFVPLD
160 170 180 190 200
YFLHPQVYYQ KQITQSGRDF IMHCFEYKDP ETGVNYLIQG MTSKLAVLVA
210 220 230
LIILEQSPAF KIDFDLHDLI PSCERTFLWR YSLSKL
Sequence cautioni
The sequence AAH33046 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 161 | K → E in AAK07483 (PubMed:11415433).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_014271 | 1 – 63 | MSRPC…VRSDK → MRVRTICFPKSPAASKRKYC RADVATLWTPGACQ in isoform 4. 1 PublicationAdd BLAST | 63 | |
Alternative sequenceiVSP_014272 | 1 – 12 | MSRPC…PEPVR → MVQSLELPQRQPCHFGSWIK RSPSGLTSKSPSQVLG in isoform 2. 2 PublicationsAdd BLAST | 12 | |
Alternative sequenceiVSP_014273 | 97 – 116 | Missing in isoform 3. 1 PublicationAdd BLAST | 20 | |
Alternative sequenceiVSP_014274 | 117 – 171 | NDALV…GRDFI → VRTGMGPPELSGASTKFPNT LGTEPGELSPCVDSVLCELC GPSHSIQEERGEPPQ in isoform 5. 1 PublicationAdd BLAST | 55 | |
Alternative sequenceiVSP_014275 | 172 – 236 | Missing in isoform 5. 1 PublicationAdd BLAST | 65 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF338424 mRNA Translation: AAK07483.1 AK004924 mRNA Translation: BAB23675.1 AK011172 mRNA Translation: BAB27446.1 AK088191 mRNA Translation: BAC40199.1 BC033046 mRNA Translation: AAH33046.1 Different initiation. BC069843 mRNA Translation: AAH69843.1 |
CCDSi | CCDS22687.1 [Q99P30-1] CCDS22688.2 [Q99P30-3] CCDS80934.1 [Q99P30-5] CCDS80935.1 [Q99P30-2] |
RefSeqi | NP_001277109.1, NM_001290180.1 NP_001277110.1, NM_001290181.1 [Q99P30-2] NP_001277111.1, NM_001290182.1 [Q99P30-5] NP_077757.2, NM_024437.4 [Q99P30-1] NP_077766.3, NM_024446.5 [Q99P30-3] |
Genome annotation databases
Ensembli | ENSMUST00000066514; ENSMUSP00000065791; ENSMUSG00000031767 [Q99P30-4] ENSMUST00000073521; ENSMUSP00000073213; ENSMUSG00000031767 [Q99P30-1] ENSMUST00000109109; ENSMUSP00000104737; ENSMUSG00000031767 [Q99P30-2] ENSMUST00000134593; ENSMUSP00000116868; ENSMUSG00000031767 [Q99P30-5] ENSMUST00000147605; ENSMUSP00000114598; ENSMUSG00000031767 [Q99P30-3] |
GeneIDi | 67528 |
KEGGi | mmu:67528 |
UCSCi | uc009nnv.3, mouse [Q99P30-5] uc009nnw.3, mouse [Q99P30-1] uc009nny.3, mouse [Q99P30-3] uc012glf.2, mouse [Q99P30-4] uc057and.1, mouse [Q99P30-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF338424 mRNA Translation: AAK07483.1 AK004924 mRNA Translation: BAB23675.1 AK011172 mRNA Translation: BAB27446.1 AK088191 mRNA Translation: BAC40199.1 BC033046 mRNA Translation: AAH33046.1 Different initiation. BC069843 mRNA Translation: AAH69843.1 |
CCDSi | CCDS22687.1 [Q99P30-1] CCDS22688.2 [Q99P30-3] CCDS80934.1 [Q99P30-5] CCDS80935.1 [Q99P30-2] |
RefSeqi | NP_001277109.1, NM_001290180.1 NP_001277110.1, NM_001290181.1 [Q99P30-2] NP_001277111.1, NM_001290182.1 [Q99P30-5] NP_077757.2, NM_024437.4 [Q99P30-1] NP_077766.3, NM_024446.5 [Q99P30-3] |
3D structure databases
SMRi | Q99P30 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | Q99P30, 2 interactors |
MINTi | Q99P30 |
STRINGi | 10090.ENSMUSP00000073213 |
Chemistry databases
SwissLipidsi | SLP:000001595 [Q99P30-1] |
PTM databases
iPTMneti | Q99P30 |
PhosphoSitePlusi | Q99P30 |
SwissPalmi | Q99P30 |
2D gel databases
REPRODUCTION-2DPAGEi | Q99P30 |
Proteomic databases
jPOSTi | Q99P30 |
PaxDbi | Q99P30 |
PeptideAtlasi | Q99P30 |
PRIDEi | Q99P30 |
Protocols and materials databases
Antibodypediai | 48153, 29 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000066514; ENSMUSP00000065791; ENSMUSG00000031767 [Q99P30-4] ENSMUST00000073521; ENSMUSP00000073213; ENSMUSG00000031767 [Q99P30-1] ENSMUST00000109109; ENSMUSP00000104737; ENSMUSG00000031767 [Q99P30-2] ENSMUST00000134593; ENSMUSP00000116868; ENSMUSG00000031767 [Q99P30-5] ENSMUST00000147605; ENSMUSP00000114598; ENSMUSG00000031767 [Q99P30-3] |
GeneIDi | 67528 |
KEGGi | mmu:67528 |
UCSCi | uc009nnv.3, mouse [Q99P30-5] uc009nnw.3, mouse [Q99P30-1] uc009nny.3, mouse [Q99P30-3] uc012glf.2, mouse [Q99P30-4] uc057and.1, mouse [Q99P30-2] |
Organism-specific databases
CTDi | 283927 |
MGIi | MGI:1914778, Nudt7 |
Phylogenomic databases
eggNOGi | KOG3069, Eukaryota |
GeneTreei | ENSGT00940000159631 |
HOGENOMi | CLU_040940_6_0_1 |
InParanoidi | Q99P30 |
OMAi | CFEYTDP |
OrthoDBi | 1253012at2759 |
PhylomeDBi | Q99P30 |
TreeFami | TF106350 |
Enzyme and pathway databases
Reactomei | R-MMU-9033241, Peroxisomal protein import |
Miscellaneous databases
BioGRID-ORCSi | 67528, 0 hits in 16 CRISPR screens |
ChiTaRSi | Nudt7, mouse |
PROi | PR:Q99P30 |
RNActi | Q99P30, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000031767, Expressed in liver and 279 other tissues |
Genevisiblei | Q99P30, MM |
Family and domain databases
InterProi | View protein in InterPro IPR015797, NUDIX_hydrolase-like_dom_sf IPR000086, NUDIX_hydrolase_dom IPR000059, NUDIX_hydrolase_NudL_CS |
Pfami | View protein in Pfam PF00293, NUDIX, 1 hit |
SUPFAMi | SSF55811, SSF55811, 1 hit |
PROSITEi | View protein in PROSITE PS51462, NUDIX, 1 hit PS01293, NUDIX_COA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NUDT7_MOUSE | |
Accessioni | Q99P30Primary (citable) accession number: Q99P30 Secondary accession number(s): Q6IS65 Q9DBI9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2005 |
Last sequence update: | July 19, 2005 | |
Last modified: | February 10, 2021 | |
This is version 130 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families