UniProtKB - Q99NB8 (UBQL4_MOUSE)
Ubiquilin-4
Ubqln4
Functioni
GO - Molecular functioni
- identical protein binding Source: MGI
- K48-linked polyubiquitin modification-dependent protein binding Source: MGI
- polyubiquitin modification-dependent protein binding Source: UniProtKB
GO - Biological processi
- autophagy Source: UniProtKB-KW
- cellular response to DNA damage stimulus Source: UniProtKB
- DNA repair Source: UniProtKB-KW
- negative regulation of autophagosome maturation Source: MGI
- negative regulation of double-strand break repair via homologous recombination Source: UniProtKB
- regulation of proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
- ubiquitin-dependent protein catabolic process Source: GO_Central
Keywordsi
Biological process | Autophagy, DNA damage, DNA repair |
Names & Taxonomyi
Protein namesi | Recommended name: Ubiquilin-4CuratedAlternative name(s): Ataxin-1 interacting ubiquitin-like proteinCurated Short name: A1UpBy similarity Ataxin-1 ubiquitin-like-interacting protein A1U Connexin43-interacting protein of 75 kDa1 Publication Short name: CIP751 Publication |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2150152, Ubqln4 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum 3 Publications
Nucleus
- Nucleus 1 Publication
Other locations
- Cytoplasm By similarity
- Chromosome By similarity
- perinuclear region 1 Publication
- autophagosome By similarity
Note: Colocalizes with the proteasome, both in nucleus and cytoplasm. Exported from the nucleus following interaction with DESI1/POST. In response to DNA damage and phosphorylation at Ser-318 by ATM, localizes to the nucleus and is recruited to sites of DNA damage.By similarity
Cytosol
- cytosol Source: MGI
- cytosolic proteasome complex Source: Ensembl
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: MGI
Nucleus
- nuclear proteasome complex Source: Ensembl
- nucleoplasm Source: MGI
- nucleus Source: UniProtKB
Other locations
- autophagosome Source: MGI
- cytoplasm Source: UniProtKB
- cytoplasmic vesicle Source: UniProtKB-KW
- perinuclear region of cytoplasm Source: UniProtKB-SubCell
- protein-containing complex Source: MGI
- site of DNA damage Source: UniProtKB
Keywords - Cellular componenti
Chromosome, Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000211016 | 1 – 596 | Ubiquilin-4Add BLAST | 596 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 23 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 62 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 139 | PhosphoserineBy similarity | 1 | |
Modified residuei | 282 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 313 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q99NB8 |
MaxQBi | Q99NB8 |
PaxDbi | Q99NB8 |
PeptideAtlasi | Q99NB8 |
PRIDEi | Q99NB8 |
PTM databases
iPTMneti | Q99NB8 |
PhosphoSitePlusi | Q99NB8 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000008604, Expressed in ventricular zone and 291 other tissues |
Genevisiblei | Q99NB8, MM |
Interactioni
Subunit structurei
Homooligomer (By similarity). Binds signal sequences of proteins that are targeted to the endoplasmic reticulum (PubMed:11162551).
Interacts (via UBA domain) with GJA1 (not ubiquitinated) and with ubiquitin; both compete for the same binding site (PubMed:18079109, PubMed:20127391, PubMed:20940304).
Interacts (via UBA domain) with ubiquitin and with polyubiquitin chains (PubMed:20940304).
Interacts (via ubiquitin-like domain) with PSMD2 and PSMD4, regulatory subunits of the 26S proteasome (PubMed:18079109).
Interacts with ATXN1/SCA1; interaction with ATXN1 inhibits polyubiquitination of UBQLN4 and interferes with PSMD4 binding (By similarity).
Interacts with HERPUD1 (By similarity).
Interacts (via ubiquitin-like domain) with UBQLN1 (via UBA domain) (By similarity).
Interacts with UBQLN2 (By similarity).
Interacts (via STI1 1 and 2 domains) with MAP1LC3A/B/C (By similarity).
Interacts with BAG6 (By similarity).
Interacts with MRE11 (when ubiquitinated); interaction with ubiquitinated MRE11 leads to MRE11 removal from chromatin (By similarity).
Interacts with DESI1/POST; leading to nuclear export (By similarity).
By similarity4 PublicationsGO - Molecular functioni
- identical protein binding Source: MGI
- K48-linked polyubiquitin modification-dependent protein binding Source: MGI
- polyubiquitin modification-dependent protein binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 220488, 48 interactors |
IntActi | Q99NB8, 4 interactors |
MINTi | Q99NB8 |
STRINGi | 10090.ENSMUSP00000008748 |
Miscellaneous databases
RNActi | Q99NB8, protein |
Structurei
Secondary structure
3D structure databases
BMRBi | Q99NB8 |
SMRi | Q99NB8 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q99NB8 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 13 – 87 | Ubiquitin-likePROSITE-ProRule annotationAdd BLAST | 75 | |
Domaini | 187 – 224 | STI1 1Sequence analysisAdd BLAST | 38 | |
Domaini | 225 – 256 | STI1 2Sequence analysisAdd BLAST | 32 | |
Domaini | 388 – 435 | STI1 3Sequence analysisAdd BLAST | 48 | |
Domaini | 439 – 471 | STI1 4Sequence analysisAdd BLAST | 33 | |
Domaini | 548 – 593 | UBAPROSITE-ProRule annotationAdd BLAST | 46 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 174 – 257 | Met-richAdd BLAST | 84 |
Phylogenomic databases
eggNOGi | KOG0010, Eukaryota |
GeneTreei | ENSGT00940000155620 |
HOGENOMi | CLU_024293_4_0_1 |
InParanoidi | Q99NB8 |
OMAi | SIGGVKM |
OrthoDBi | 1553668at2759 |
PhylomeDBi | Q99NB8 |
TreeFami | TF314412 |
Family and domain databases
InterProi | View protein in InterPro IPR006636, STI1_HS-bd IPR015940, UBA IPR009060, UBA-like_sf IPR015496, Ubiquilin IPR000626, Ubiquitin-like_dom IPR029071, Ubiquitin-like_domsf |
PANTHERi | PTHR10677, PTHR10677, 1 hit |
Pfami | View protein in Pfam PF00627, UBA, 1 hit PF00240, ubiquitin, 1 hit |
SMARTi | View protein in SMART SM00727, STI1, 4 hits SM00165, UBA, 1 hit SM00213, UBQ, 1 hit |
SUPFAMi | SSF46934, SSF46934, 1 hit SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS50030, UBA, 1 hit PS50053, UBIQUITIN_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MAEPSGAETR PQIRVTVKTP KDKEEIVICD QASVKEFKEE ISRRFKAQQD
60 70 80 90 100
QLVLIFAGKI LKDGDTLSQH GIKDGLTVHL VIKTPQKAQD PVTAAASPPS
110 120 130 140 150
TPDSASAPST TPASPAAAPV QPCSSGNTTS DAGSGGGPSP VAAEGPSSAT
160 170 180 190 200
ASILSGFGGI LGLGSLGLGS ANFMELQQQM QRQLMSNPEM LSQIMENPLV
210 220 230 240 250
QDMMSNPDLM RHMIMANPQM QQLMERNPEI SHMLNNPELM RQTMELARNP
260 270 280 290 300
AMMQEMMRNQ DRALSNLESV PGGYNALRRM YTDIQEPMFT AAREQFGNNP
310 320 330 340 350
FSSLAGNSDN SSSQPLRTEN REPLPNPWSP SPPTSQAPGS GGEGTGGSGT
360 370 380 390 400
SQVHPTVSNP FGINAASLGS GMFNSPEMQA LLQQISENPQ LMQNVISAPY
410 420 430 440 450
MRTMMQTLAQ NPDFAAQMMV NVPLFAGNPQ LQEQLRLQLP VFLQQMQNPE
460 470 480 490 500
SLSILTNPRA MQALLQIQQG LQTLQTEAPG LVPSLGSFGT PRTSVPLAGS
510 520 530 540 550
NSGSSAEAPT SSPGVPATSP PSAGSNAQQQ LMQQMIQLLS GSGNSQVPMP
560 570 580 590
EVRFQQQLEQ LNSMGFINRE ANLQALIATG GDINAAIERL LGSQLS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0A6YXU5 | A0A0A6YXU5_MOUSE | Ubiquilin-4 | Ubqln4 | 45 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB040050 mRNA Translation: BAB40326.1 BC017686 mRNA Translation: AAH17686.1 AK077511 mRNA Translation: BAC36837.1 |
CCDSi | CCDS17479.1 |
RefSeqi | NP_277068.1, NM_033526.2 |
Genome annotation databases
Ensembli | ENSMUST00000008748; ENSMUSP00000008748; ENSMUSG00000008604 |
GeneIDi | 94232 |
KEGGi | mmu:94232 |
UCSCi | uc008pvq.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB040050 mRNA Translation: BAB40326.1 BC017686 mRNA Translation: AAH17686.1 AK077511 mRNA Translation: BAC36837.1 |
CCDSi | CCDS17479.1 |
RefSeqi | NP_277068.1, NM_033526.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2KNZ | NMR | - | A | 549-596 | [»] | |
BMRBi | Q99NB8 | |||||
SMRi | Q99NB8 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 220488, 48 interactors |
IntActi | Q99NB8, 4 interactors |
MINTi | Q99NB8 |
STRINGi | 10090.ENSMUSP00000008748 |
PTM databases
iPTMneti | Q99NB8 |
PhosphoSitePlusi | Q99NB8 |
Proteomic databases
EPDi | Q99NB8 |
MaxQBi | Q99NB8 |
PaxDbi | Q99NB8 |
PeptideAtlasi | Q99NB8 |
PRIDEi | Q99NB8 |
Protocols and materials databases
Antibodypediai | 34204, 103 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000008748; ENSMUSP00000008748; ENSMUSG00000008604 |
GeneIDi | 94232 |
KEGGi | mmu:94232 |
UCSCi | uc008pvq.1, mouse |
Organism-specific databases
CTDi | 56893 |
MGIi | MGI:2150152, Ubqln4 |
Phylogenomic databases
eggNOGi | KOG0010, Eukaryota |
GeneTreei | ENSGT00940000155620 |
HOGENOMi | CLU_024293_4_0_1 |
InParanoidi | Q99NB8 |
OMAi | SIGGVKM |
OrthoDBi | 1553668at2759 |
PhylomeDBi | Q99NB8 |
TreeFami | TF314412 |
Miscellaneous databases
BioGRID-ORCSi | 94232, 1 hit in 17 CRISPR screens |
ChiTaRSi | Ubqln4, mouse |
EvolutionaryTracei | Q99NB8 |
PROi | PR:Q99NB8 |
RNActi | Q99NB8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000008604, Expressed in ventricular zone and 291 other tissues |
Genevisiblei | Q99NB8, MM |
Family and domain databases
InterProi | View protein in InterPro IPR006636, STI1_HS-bd IPR015940, UBA IPR009060, UBA-like_sf IPR015496, Ubiquilin IPR000626, Ubiquitin-like_dom IPR029071, Ubiquitin-like_domsf |
PANTHERi | PTHR10677, PTHR10677, 1 hit |
Pfami | View protein in Pfam PF00627, UBA, 1 hit PF00240, ubiquitin, 1 hit |
SMARTi | View protein in SMART SM00727, STI1, 4 hits SM00165, UBA, 1 hit SM00213, UBQ, 1 hit |
SUPFAMi | SSF46934, SSF46934, 1 hit SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS50030, UBA, 1 hit PS50053, UBIQUITIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | UBQL4_MOUSE | |
Accessioni | Q99NB8Primary (citable) accession number: Q99NB8 Secondary accession number(s): Q8BP88 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 15, 2004 |
Last sequence update: | June 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 159 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references