UniProtKB - Q99NA9 (PCGF6_MOUSE)
Protein
Polycomb group RING finger protein 6
Gene
Pcgf6
Organism
Mus musculus (Mouse)
Status
Functioni
Transcriptional repressor. May modulate the levels of histone H3K4Me3 by activating KDM5D histone demethylase. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Within the PRC1-like complex, regulates RNF2 ubiquitin ligase activity.By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 137 – 176 | RING-typePROSITE-ProRule annotationAdd BLAST | 40 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- protein domain specific binding Source: MGI
GO - Biological processi
- histone H2A-K119 monoubiquitination Source: UniProtKB
- negative regulation of transcription, DNA-templated Source: MGI
- negative regulation of transcription by RNA polymerase II Source: NTNU_SB
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | Repressor |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-8953750, Transcriptional Regulation by E2F6 |
Names & Taxonomyi
Protein namesi | Recommended name: Polycomb group RING finger protein 6Alternative name(s): Mel18 and Bmi1-like RING finger RING finger protein 134 |
Gene namesi | Name:Pcgf6 Synonyms:Mblr, Rnf134 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1918291, Pcgf6 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Nucleus
- nucleus Source: UniProtKB
- PcG protein complex Source: UniProtKB
- PRC1 complex Source: UniProtKB
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000055990 | 1 – 353 | Polycomb group RING finger protein 6Add BLAST | 353 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 34 | PhosphoserineBy similarity | 1 | |
Modified residuei | 118 | PhosphoserineCombined sources | 1 | |
Cross-linki | 226 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 237 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity |
Post-translational modificationi
Phosphorylated during mitosis.By similarity
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q99NA9 |
MaxQBi | Q99NA9 |
PaxDbi | Q99NA9 |
PeptideAtlasi | Q99NA9 |
PRIDEi | Q99NA9 |
PTM databases
iPTMneti | Q99NA9 |
PhosphoSitePlusi | Q99NA9 |
Expressioni
Tissue specificityi
Expressed in ovary, testis, stomach, liver, thymus and kidney (at protein level).1 Publication
Developmental stagei
Expressed in embryo at 10.5 dpc.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000025050, Expressed in primary oocyte and 282 other tissues |
ExpressionAtlasi | Q99NA9, baseline and differential |
Genevisiblei | Q99NA9, MM |
Interactioni
Subunit structurei
Component of a PRC1-like complex.
Interacts with BMI1/PCGF4, RING1 and RNF2.
Interacts with KDM5D.
Interacts with CBX4, CBX6, CBX7 and CBX8.
By similarityGO - Molecular functioni
- protein domain specific binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 214431, 7 interactors |
IntActi | Q99NA9, 6 interactors |
MINTi | Q99NA9 |
STRINGi | 10090.ENSMUSP00000026032 |
Miscellaneous databases
RNActi | Q99NA9, protein |
Family & Domainsi
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 71 – 112 | Sequence analysisAdd BLAST | 42 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 24 – 42 | Pro-richAdd BLAST | 19 | |
Compositional biasi | 82 – 123 | Glu-richAdd BLAST | 42 |
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 137 – 176 | RING-typePROSITE-ProRule annotationAdd BLAST | 40 |
Keywords - Domaini
Coiled coil, Zinc-fingerPhylogenomic databases
eggNOGi | KOG2660, Eukaryota |
GeneTreei | ENSGT00940000158034 |
HOGENOMi | CLU_046427_4_0_1 |
InParanoidi | Q99NA9 |
OMAi | YPYIRCA |
OrthoDBi | 1203951at2759 |
PhylomeDBi | Q99NA9 |
TreeFami | TF324206 |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR038037, PCGF6 IPR029071, Ubiquitin-like_domsf IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
PANTHERi | PTHR10825:SF74, PTHR10825:SF74, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit |
SUPFAMi | SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q99NA9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDEAETDATE NKRASEAKRA SAMPPPPPPP PPISPPALIP APAAGEEGPA
60 70 80 90 100
SLGQAGAAGC SRSRPPALEP ERSLGRLRGR FEDYDEELEE EEEMEEEEEE
110 120 130 140 150
EEEMSHFSLR LESGRADSED EEERLINLVE LTPYILCSIC KGYLIDATTI
160 170 180 190 200
TECLHTFCKS CIVRHFYYSN RCPKCNIVVH QTQPLYNIRL DRQLQDIVYK
210 220 230 240 250
LVINLEEREK KQMHDFYKER GLEVPKPAAP QPVPSSKGKT KKVLESVFRI
260 270 280 290 300
PPELDMSLLL EFIGANEDTG HFKPLEKKFV RVSGEATIGH VEKFLRRKMG
310 320 330 340 350
LDPACQVDII CGDHLLERYQ TLREIRRAIG DTAMQDGLLV LHYGLVVSPL
KIT
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A494B9Z3 | A0A494B9Z3_MOUSE | Polycomb group RING finger protein ... | Pcgf6 | 239 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB047007 mRNA Translation: BAB40780.1 BC016195 mRNA Translation: AAH16195.1 BC089460 mRNA Translation: AAH89460.1 |
CCDSi | CCDS29885.1 |
RefSeqi | NP_081930.1, NM_027654.3 |
Genome annotation databases
Ensembli | ENSMUST00000026032; ENSMUSP00000026032; ENSMUSG00000025050 |
GeneIDi | 71041 |
KEGGi | mmu:71041 |
UCSCi | uc008hul.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB047007 mRNA Translation: BAB40780.1 BC016195 mRNA Translation: AAH16195.1 BC089460 mRNA Translation: AAH89460.1 |
CCDSi | CCDS29885.1 |
RefSeqi | NP_081930.1, NM_027654.3 |
3D structure databases
BMRBi | Q99NA9 |
SMRi | Q99NA9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 214431, 7 interactors |
IntActi | Q99NA9, 6 interactors |
MINTi | Q99NA9 |
STRINGi | 10090.ENSMUSP00000026032 |
PTM databases
iPTMneti | Q99NA9 |
PhosphoSitePlusi | Q99NA9 |
Proteomic databases
EPDi | Q99NA9 |
MaxQBi | Q99NA9 |
PaxDbi | Q99NA9 |
PeptideAtlasi | Q99NA9 |
PRIDEi | Q99NA9 |
Protocols and materials databases
Antibodypediai | 31521, 152 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000026032; ENSMUSP00000026032; ENSMUSG00000025050 |
GeneIDi | 71041 |
KEGGi | mmu:71041 |
UCSCi | uc008hul.1, mouse |
Organism-specific databases
CTDi | 84108 |
MGIi | MGI:1918291, Pcgf6 |
Phylogenomic databases
eggNOGi | KOG2660, Eukaryota |
GeneTreei | ENSGT00940000158034 |
HOGENOMi | CLU_046427_4_0_1 |
InParanoidi | Q99NA9 |
OMAi | YPYIRCA |
OrthoDBi | 1203951at2759 |
PhylomeDBi | Q99NA9 |
TreeFami | TF324206 |
Enzyme and pathway databases
Reactomei | R-MMU-8953750, Transcriptional Regulation by E2F6 |
Miscellaneous databases
BioGRID-ORCSi | 71041, 3 hits in 20 CRISPR screens |
ChiTaRSi | Pcgf6, mouse |
PROi | PR:Q99NA9 |
RNActi | Q99NA9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000025050, Expressed in primary oocyte and 282 other tissues |
ExpressionAtlasi | Q99NA9, baseline and differential |
Genevisiblei | Q99NA9, MM |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR038037, PCGF6 IPR029071, Ubiquitin-like_domsf IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
PANTHERi | PTHR10825:SF74, PTHR10825:SF74, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit |
SUPFAMi | SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PCGF6_MOUSE | |
Accessioni | Q99NA9Primary (citable) accession number: Q99NA9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 11, 2005 |
Last sequence update: | June 1, 2001 | |
Last modified: | February 10, 2021 | |
This is version 145 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot