UniProtKB - Q99NA2 (ATOH8_MOUSE)
Transcription factor Atoh8
Atoh8
Functioni
Transcription factor that binds a palindromic (canonical) core consensus DNA sequence 5'-CANNTG- 3' known as an E-box element, possibly as a heterodimer with other bHLH proteins (By similarity).
Regulates endothelial cell proliferation, migration and tube-like structures formation (By similarity).
Modulates endothelial cell differentiation through NOS3 (By similarity).
May be implicated in specification and differentiation of neuronal cell lineages in the brain (PubMed:11733035).
May participate in kidney development and may be involved in podocyte differentiation (PubMed:16937370).
During early embryonic development is involved in tissue-specific differentiation processes that are dependent on class II bHLH factors and namely modulates the differentiation program initiated by the pro-endocrine factor NEUROG3 (PubMed:18560595).
During myogenesis, may play a role during the transition of myoblasts from the proliferative phase to the differentiation phase (PubMed:24186058).
Positively regulates HAMP transcription in two ways, firstly by acting directly on the HAMP promoter via E-boxes binding and indirectly through increased phosphorylation of SMAD protein complex (By similarity).
Repress NEUROG3-dependent gene activation in a gene-specific manner through at least two mechanisms; requires only either the sequestering of a general partner such as TCF3 through heterodimerization, either also requires binding of the bHLH domain to DNA via a basic motif (PubMed:23938248).
By similarity4 PublicationsCaution
GO - Molecular functioni
- DNA-binding transcription factor activity Source: UniProtKB
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- DNA-binding transcription factor binding Source: UniProtKB
- E-box binding Source: UniProtKB
- protein dimerization activity Source: InterPro
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- transcription factor binding Source: UniProtKB
GO - Biological processi
- formation of primary germ layer Source: UniProtKB
- myoblast proliferation Source: UniProtKB
- negative regulation of endothelial cell proliferation Source: UniProtKB
- negative regulation of gene expression Source: MGI
- negative regulation of transcription, DNA-templated Source: UniProtKB
- neuron differentiation Source: GO_Central
- positive regulation of endothelial cell differentiation Source: UniProtKB
- positive regulation of endothelial cell migration Source: UniProtKB
- positive regulation of miRNA transcription Source: MGI
- positive regulation of transcription, DNA-templated Source: UniProtKB
- regulation of transcription by RNA polymerase II Source: GO_Central
- SMAD protein signal transduction Source: UniProtKB
- tube formation Source: UniProtKB
Keywordsi
Molecular function | Developmental protein, DNA-binding |
Biological process | Differentiation, Neurogenesis, Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription factor Atoh8CuratedAlternative name(s): Helix-loop-helix protein mATH-61 Publication Short name: mATH61 Publication Okadin1 Publication Protein atonal homolog 8Imported |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1918343, Atoh8 |
VEuPathDBi | HostDB:ENSMUSG00000037621 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
- Nucleus speckle By similarity
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Nucleus
- nuclear speck Source: UniProtKB-SubCell
- nucleoplasm Source: MGI
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000323752 | 1 – 322 | Transcription factor Atoh8Add BLAST | 322 |
Proteomic databases
PaxDbi | Q99NA2 |
PRIDEi | Q99NA2 |
PTM databases
iPTMneti | Q99NA2 |
PhosphoSitePlusi | Q99NA2 |
Expressioni
Tissue specificityi
Developmental stagei
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000037621, Expressed in testis and 144 other tissues |
ExpressionAtlasi | Q99NA2, baseline and differential |
Genevisiblei | Q99NA2, MM |
Interactioni
Subunit structurei
Efficient DNA binding requires dimerization with another bHLH protein.
Interacts with NEUROG3 AND NEUROD1.
Interacts with ZFPM2; mediates indirect interaction with GATA4.
Forms a heterodimer with TCF3; repress transcription of TCF3 and TCF3/NEUROG3 dimer-induced transactivation of E box-dependent promoters.
By similarity3 PublicationsGO - Molecular functioni
- DNA-binding transcription factor binding Source: UniProtKB
- protein dimerization activity Source: InterPro
- transcription factor binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 214472, 1 interactor |
IntActi | Q99NA2, 1 interactor |
STRINGi | 10090.ENSMUSP00000036981 |
Miscellaneous databases
RNActi | Q99NA2, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 231 – 283 | bHLHPROSITE-ProRule annotationAdd BLAST | 53 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 77 – 96 | DisorderedSequence analysisAdd BLAST | 20 | |
Regioni | 101 – 144 | DisorderedSequence analysisAdd BLAST | 44 | |
Regioni | 159 – 221 | DisorderedSequence analysisAdd BLAST | 63 | |
Regioni | 231 – 244 | Basic motif; degeneratePROSITE-ProRule annotationAdd BLAST | 14 | |
Regioni | 245 – 283 | Helix-loop-helix motifPROSITE-ProRule annotationAdd BLAST | 39 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 121 – 135 | Pro residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 164 – 186 | Pro residuesSequence analysisAdd BLAST | 23 | |
Compositional biasi | 188 – 209 | Polar residuesSequence analysisAdd BLAST | 22 |
Domaini
Phylogenomic databases
eggNOGi | KOG3898, Eukaryota |
GeneTreei | ENSGT00940000161151 |
HOGENOMi | CLU_057987_0_0_1 |
InParanoidi | Q99NA2 |
OMAi | VCVQELH |
OrthoDBi | 1014108at2759 |
PhylomeDBi | Q99NA2 |
TreeFami | TF324848 |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR032660, ATOH8 IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf |
PANTHERi | PTHR19290:SF102, PTHR19290:SF102, 1 hit |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MKHIPVLEDG PWKTVCVKEL NGLKKLKRKG KEPVRRANGY KTFRLDLEAP
60 70 80 90 100
ELGATVSTTA ATNGLRDRTQ PFPIATPVPA SVAPAVPPGG GTDTAREFRG
110 120 130 140 150
IRAPEVSDAR KRGFALGTVG PGLPTPPPPP ASQSLAPGDP EAHSFREQAL
160 170 180 190 200
RPRILLCAPP ARPTQSAPLA PPAAPQESPV RPAPPTRPGE SSYSSISHVI
210 220 230 240 250
YNNHPDSSAS PRKRPGEATA ASTEIKALQQ TRRLLANARE RTRVHTISAA
260 270 280 290 300
FEALRKQVPC YSYGQKLSKL AILRIACNYI LSLARLADLD YSADHSNLSF
310 320
SECVQRCTRT LQAEGRAKKR KE
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0U1RP16 | A0A0U1RP16_MOUSE | Transcription factor Atoh8 | Atoh8 | 114 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 213 | K → E in BAC25499 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB046527 mRNA Translation: BAC57617.1 AB046528 mRNA Translation: BAC57618.1 AB049066 mRNA Translation: BAB39468.1 AY349615 mRNA Translation: AAQ54689.1 BC023684 mRNA Translation: AAH23684.1 AK016909 mRNA Translation: BAC25499.1 |
CCDSi | CCDS39511.1 |
RefSeqi | NP_722473.1, NM_153778.3 |
Genome annotation databases
Ensembli | ENSMUST00000042646; ENSMUSP00000036981; ENSMUSG00000037621 |
GeneIDi | 71093 |
KEGGi | mmu:71093 |
UCSCi | uc009cic.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB046527 mRNA Translation: BAC57617.1 AB046528 mRNA Translation: BAC57618.1 AB049066 mRNA Translation: BAB39468.1 AY349615 mRNA Translation: AAQ54689.1 BC023684 mRNA Translation: AAH23684.1 AK016909 mRNA Translation: BAC25499.1 |
CCDSi | CCDS39511.1 |
RefSeqi | NP_722473.1, NM_153778.3 |
3D structure databases
AlphaFoldDBi | Q99NA2 |
SMRi | Q99NA2 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 214472, 1 interactor |
IntActi | Q99NA2, 1 interactor |
STRINGi | 10090.ENSMUSP00000036981 |
PTM databases
iPTMneti | Q99NA2 |
PhosphoSitePlusi | Q99NA2 |
Proteomic databases
PaxDbi | Q99NA2 |
PRIDEi | Q99NA2 |
Protocols and materials databases
Antibodypediai | 17011, 135 antibodies from 26 providers |
DNASUi | 71093 |
Genome annotation databases
Ensembli | ENSMUST00000042646; ENSMUSP00000036981; ENSMUSG00000037621 |
GeneIDi | 71093 |
KEGGi | mmu:71093 |
UCSCi | uc009cic.2, mouse |
Organism-specific databases
CTDi | 84913 |
MGIi | MGI:1918343, Atoh8 |
VEuPathDBi | HostDB:ENSMUSG00000037621 |
Phylogenomic databases
eggNOGi | KOG3898, Eukaryota |
GeneTreei | ENSGT00940000161151 |
HOGENOMi | CLU_057987_0_0_1 |
InParanoidi | Q99NA2 |
OMAi | VCVQELH |
OrthoDBi | 1014108at2759 |
PhylomeDBi | Q99NA2 |
TreeFami | TF324848 |
Miscellaneous databases
BioGRID-ORCSi | 71093, 0 hits in 71 CRISPR screens |
ChiTaRSi | Atoh8, mouse |
PROi | PR:Q99NA2 |
RNActi | Q99NA2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000037621, Expressed in testis and 144 other tissues |
ExpressionAtlasi | Q99NA2, baseline and differential |
Genevisiblei | Q99NA2, MM |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR032660, ATOH8 IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf |
PANTHERi | PTHR19290:SF102, PTHR19290:SF102, 1 hit |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ATOH8_MOUSE | |
Accessioni | Q99NA2Primary (citable) accession number: Q99NA2 Secondary accession number(s): Q8C1I7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 18, 2008 |
Last sequence update: | June 1, 2001 | |
Last modified: | May 25, 2022 | |
This is version 133 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot