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Protein

Leupaxin

Gene

Lpxn

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional coactivator for androgen receptor (AR) and serum response factor (SRF). Contributes to the regulation of cell adhesion, spreading and cell migration and acts as a negative regulator in integrin-mediated cell adhesion events. Suppresses the integrin-induced tyrosine phosphorylation of paxillin (PXN). May play a critical role as an adapter protein in the formation of the adhesion zone in osteoclasts. Negatively regulates B-cell antigen receptor (BCR) signaling.4 Publications

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • transcription coregulator activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionActivator
Biological processCell adhesion, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Leupaxin
Gene namesi
Name:Lpxn
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:2147677 Lpxn

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000758371 – 386LeupaxinAdd BLAST386

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei19PhosphoserineBy similarity1
Modified residuei22PhosphotyrosineCombined sources1
Modified residuei54PhosphoserineCombined sources1
Modified residuei62PhosphotyrosineCombined sources1
Modified residuei72Phosphotyrosine; by LYNCombined sources1 Publication1
Modified residuei81PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated on tyrosine residues. Phosphorylation on Tyr-72 is important for its inhibitory function. Bombesin stimulates phosphorylation on Tyr-22, Tyr-62 and Tyr-72 (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ99N69
MaxQBiQ99N69
PaxDbiQ99N69
PeptideAtlasiQ99N69
PRIDEiQ99N69

PTM databases

iPTMnetiQ99N69
PhosphoSitePlusiQ99N69

Expressioni

Tissue specificityi

Expressed in osteoclasts (at protein level). Highly expressed in vascular smooth muscle.2 Publications

Gene expression databases

BgeeiENSMUSG00000024696 Expressed in 86 organ(s), highest expression level in bone marrow macrophage
CleanExiMM_LPXN
GenevisibleiQ99N69 MM

Interactioni

Subunit structurei

Interacts with unphosphorylated ITGA4. Interacts with AR and SRF (By similarity). Interacts with PTK2B/PYK2, PTPN22 and PTPN12. Interacts (via LD motif 3) with LYN and the interaction is induced upon B-cell antigen receptor (BCR) activation. Interacts (via LD motif 3) with PTK2/FAK.By similarity4 Publications

Protein-protein interaction databases

BioGridi223241, 2 interactors
IntActiQ99N69, 2 interactors
STRINGi10090.ENSMUSP00000025601

Structurei

3D structure databases

ProteinModelPortaliQ99N69
SMRiQ99N69
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini150 – 208LIM zinc-binding 1PROSITE-ProRule annotationAdd BLAST59
Domaini209 – 267LIM zinc-binding 2PROSITE-ProRule annotationAdd BLAST59
Domaini268 – 326LIM zinc-binding 3PROSITE-ProRule annotationAdd BLAST59
Domaini327 – 386LIM zinc-binding 4PROSITE-ProRule annotationAdd BLAST60

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi3 – 15LD motif 1Add BLAST13
Motifi70 – 82LD motif 2Add BLAST13
Motifi92 – 103LD motif 3Add BLAST12

Domaini

The LIM domain 3 is critical for focal adhesion targeting and the suppression of paxillin (PXN) tyrosine phosphorylation. The LIM domain 3 alone or both LIM domains 3 and 4 can mediate interaction with AR.1 Publication

Sequence similaritiesi

Belongs to the paxillin family.Curated

Keywords - Domaini

LIM domain, Repeat

Phylogenomic databases

eggNOGiKOG1703 Eukaryota
ENOG410XRD4 LUCA
GeneTreeiENSGT00760000118910
HOGENOMiHOG000018764
HOVERGENiHBG001512
InParanoidiQ99N69
OMAiELMAHLC
OrthoDBiEOG091G05AW
PhylomeDBiQ99N69
TreeFamiTF314113

Family and domain databases

InterProiView protein in InterPro
IPR034961 Leupaxin
IPR017305 Tgfb1i1/Leupaxin/TGFB1I1
IPR001781 Znf_LIM
PANTHERiPTHR24216:SF23 PTHR24216:SF23, 1 hit
PfamiView protein in Pfam
PF00412 LIM, 4 hits
PIRSFiPIRSF037881 Leupaxin, 1 hit
SMARTiView protein in SMART
SM00132 LIM, 4 hits
PROSITEiView protein in PROSITE
PS00478 LIM_DOMAIN_1, 4 hits
PS50023 LIM_DOMAIN_2, 4 hits

Sequencei

Sequence statusi: Complete.

Q99N69-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEELDALLEE LERCTFQDSE EYSNPVSCHL DQQSTEESKI PQTPKTLSSQ
60 70 80 90 100
GNTSPLKVQL VYATNIQEPN VYSEVQEPKE SVLPPKTSAA AQLDELMAHL
110 120 130 140 150
SEMQAKVSVK ADTSRKPLPD QQDHKASLDS MLGDLEQELQ DLGIATVPKG
160 170 180 190 200
YCASCQKPIA GKVIHALGQS WHPEHFVCTH CKEELGSSPF FERSGLAYCS
210 220 230 240 250
KDYHRLFSPR CAYCAAPITD KVLTAMNKTW HPEHFFCSHC GEVFGAEGFH
260 270 280 290 300
EKDKKPYCRK DFLAMFSPKC GGCNRPVLEN YLSAMNTVWH PECFVCGDCF
310 320 330 340 350
SSFSSGSFFE LDGRPFCELH YHHRRGTLCH DCGQPITGRC ISAMGHKFHP
360 370 380
EHFVCAFCLT QLPKGIFKEQ NNKTYCEKCF TKLFSQ
Length:386
Mass (Da):43,478
Last modified:October 1, 2002 - v2
Checksum:iAA7EB76AEF5983C7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB053936 mRNA Translation: BAB40667.2
AB071194 mRNA Translation: BAC22615.1
CCDSiCCDS29638.1
RefSeqiNP_598913.1, NM_134152.3
UniGeneiMm.313136

Genome annotation databases

EnsembliENSMUST00000025601; ENSMUSP00000025601; ENSMUSG00000024696
GeneIDi107321
KEGGimmu:107321
UCSCiuc008guq.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB053936 mRNA Translation: BAB40667.2
AB071194 mRNA Translation: BAC22615.1
CCDSiCCDS29638.1
RefSeqiNP_598913.1, NM_134152.3
UniGeneiMm.313136

3D structure databases

ProteinModelPortaliQ99N69
SMRiQ99N69
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi223241, 2 interactors
IntActiQ99N69, 2 interactors
STRINGi10090.ENSMUSP00000025601

PTM databases

iPTMnetiQ99N69
PhosphoSitePlusiQ99N69

Proteomic databases

EPDiQ99N69
MaxQBiQ99N69
PaxDbiQ99N69
PeptideAtlasiQ99N69
PRIDEiQ99N69

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025601; ENSMUSP00000025601; ENSMUSG00000024696
GeneIDi107321
KEGGimmu:107321
UCSCiuc008guq.1 mouse

Organism-specific databases

CTDi9404
MGIiMGI:2147677 Lpxn

Phylogenomic databases

eggNOGiKOG1703 Eukaryota
ENOG410XRD4 LUCA
GeneTreeiENSGT00760000118910
HOGENOMiHOG000018764
HOVERGENiHBG001512
InParanoidiQ99N69
OMAiELMAHLC
OrthoDBiEOG091G05AW
PhylomeDBiQ99N69
TreeFamiTF314113

Miscellaneous databases

PROiPR:Q99N69
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024696 Expressed in 86 organ(s), highest expression level in bone marrow macrophage
CleanExiMM_LPXN
GenevisibleiQ99N69 MM

Family and domain databases

InterProiView protein in InterPro
IPR034961 Leupaxin
IPR017305 Tgfb1i1/Leupaxin/TGFB1I1
IPR001781 Znf_LIM
PANTHERiPTHR24216:SF23 PTHR24216:SF23, 1 hit
PfamiView protein in Pfam
PF00412 LIM, 4 hits
PIRSFiPIRSF037881 Leupaxin, 1 hit
SMARTiView protein in SMART
SM00132 LIM, 4 hits
PROSITEiView protein in PROSITE
PS00478 LIM_DOMAIN_1, 4 hits
PS50023 LIM_DOMAIN_2, 4 hits
ProtoNetiSearch...

Entry informationi

Entry nameiLPXN_MOUSE
AccessioniPrimary (citable) accession number: Q99N69
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: October 1, 2002
Last modified: November 7, 2018
This is version 128 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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