UniProtKB - Q99N57 (RAF1_MOUSE)
RAF proto-oncogene serine/threonine-protein kinase
Raf1
Functioni
Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation (By similarity).
Regulates Rho signaling and migration, and is required for normal wound healing.
By similarity1 PublicationCatalytic activityi
- EC:2.7.11.1By similarityThis reaction proceeds in the forwardBy similarity direction.
- EC:2.7.11.1By similarityThis reaction proceeds in the forwardBy similarity direction.
Cofactori
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 139 | Zinc 1By similarity | 1 | |
Metal bindingi | 152 | Zinc 2By similarity | 1 | |
Metal bindingi | 155 | Zinc 2By similarity | 1 | |
Metal bindingi | 165 | Zinc 1By similarity | 1 | |
Metal bindingi | 168 | Zinc 1By similarity | 1 | |
Metal bindingi | 173 | Zinc 2By similarity | 1 | |
Metal bindingi | 176 | Zinc 2By similarity | 1 | |
Metal bindingi | 184 | Zinc 1By similarity | 1 | |
Binding sitei | 375 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 468 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 138 – 184 | Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST | 47 | |
Nucleotide bindingi | 355 – 363 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- adenylate cyclase activator activity Source: MGI
- adenylate cyclase binding Source: MGI
- ATP binding Source: MGI
- enzyme binding Source: MGI
- identical protein binding Source: MGI
- MAP kinase kinase kinase activity Source: MGI
- metal ion binding Source: UniProtKB-KW
- mitogen-activated protein kinase kinase binding Source: MGI
- protein-containing complex binding Source: MGI
- protein kinase activity Source: GO_Central
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine/threonine kinase activity Source: MGI
- protein serine kinase activity Source: RHEA
- small GTPase binding Source: MGI
GO - Biological processi
- activation of adenylate cyclase activity Source: MGI
- cell differentiation Source: MGI
- cellular glucose homeostasis Source: MGI
- death-inducing signaling complex assembly Source: MGI
- face development Source: MGI
- insulin secretion involved in cellular response to glucose stimulus Source: MGI
- intermediate filament cytoskeleton organization Source: MGI
- intracellular signal transduction Source: MGI
- MAPK cascade Source: MGI
- negative regulation of apoptotic process Source: MGI
- negative regulation of cell population proliferation Source: MGI
- negative regulation of extrinsic apoptotic signaling pathway via death domain receptors Source: MGI
- negative regulation of protein-containing complex assembly Source: MGI
- neurotrophin TRK receptor signaling pathway Source: MGI
- positive regulation of MAPK cascade Source: MGI
- positive regulation of peptidyl-serine phosphorylation Source: MGI
- positive regulation of protein phosphorylation Source: MGI
- positive regulation of transcription by RNA polymerase II Source: MGI
- protein phosphorylation Source: MGI
- response to hypoxia Source: MGI
- response to muscle stretch Source: MGI
- somatic stem cell population maintenance Source: MGI
- thymus development Source: MGI
- thyroid gland development Source: MGI
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, Metal-binding, Nucleotide-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-2672351, Stimuli-sensing channels R-MMU-392517, Rap1 signalling R-MMU-430116, GP1b-IX-V activation signalling R-MMU-5621575, CD209 (DC-SIGN) signaling R-MMU-5673000, RAF activation R-MMU-5674135, MAP2K and MAPK activation R-MMU-5674499, Negative feedback regulation of MAPK pathway R-MMU-5675221, Negative regulation of MAPK pathway |
Names & Taxonomyi
Protein namesi | Recommended name: RAF proto-oncogene serine/threonine-protein kinase (EC:2.7.11.1By similarity)Alternative name(s): Proto-oncogene c-RAF Short name: cRaf Raf-1 |
Gene namesi | Name:Raf1 Synonyms:Craf |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:97847, Raf1 |
VEuPathDBi | HostDB:ENSMUSG00000000441 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Mitochondrion
- Mitochondrion By similarity
Cytoplasm and Cytosol
- Cytoplasm By similarity
Plasma membrane
- Cell membrane By similarity
Note: Colocalizes with RGS14 and BRAF in both the cytoplasm and membranes. Phosphorylation at Ser-259 impairs its membrane accumulation. Recruited to the cell membrane by the active Ras protein. Phosphorylation at Ser-338 and Ser-339 by PAK1 is required for its mitochondrial localization (By similarity). Retinoic acid-induced Ser-621 phosphorylated form of RAF1 is predominantly localized at the nucleus.By similarity
Cytosol
- cytosol Source: MGI
Golgi apparatus
- Golgi apparatus Source: MGI
Mitochondrion
- mitochondrion Source: GO_Central
Nucleus
- nuclear speck Source: MGI
Plasma Membrane
- plasma membrane Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
- pseudopodium Source: UniProtKB
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Membrane, Mitochondrion, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000086597 | 1 – 648 | RAF proto-oncogene serine/threonine-protein kinaseAdd BLAST | 648 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 29 | Phosphoserine; by MAPK11 Publication | 1 | |
Modified residuei | 43 | Phosphoserine; by PKA and MAPK11 Publication | 1 | |
Modified residuei | 233 | Phosphoserine; by PKABy similarity | 1 | |
Modified residuei | 252 | PhosphoserineBy similarity | 1 | |
Modified residuei | 259 | Phosphoserine; by PKA, PKC and PKB/AKT1By similarity | 1 | |
Modified residuei | 268 | Phosphothreonine; by autocatalysisBy similarity | 1 | |
Modified residuei | 269 | Phosphothreonine; by PKABy similarity | 1 | |
Modified residuei | 289 | Phosphoserine; by MAPK11 Publication | 1 | |
Modified residuei | 296 | Phosphoserine; by MAPK1Combined sources1 Publication | 1 | |
Modified residuei | 301 | Phosphoserine; by MAPK1Combined sources1 Publication | 1 | |
Modified residuei | 338 | Phosphoserine; by PAK1, PAK2, PAK3 and PAK5By similarity | 1 | |
Modified residuei | 339 | Phosphoserine; by PAK1, PAK2 and PAK3By similarity | 1 | |
Modified residuei | 340 | Phosphotyrosine; by SRCBy similarity | 1 | |
Modified residuei | 341 | Phosphotyrosine; by SRCBy similarity | 1 | |
Modified residuei | 471 | PhosphoserineBy similarity | 1 | |
Modified residuei | 491 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 494 | PhosphoserineBy similarity | 1 | |
Modified residuei | 497 | Phosphoserine; by PKCBy similarity | 1 | |
Modified residuei | 499 | Phosphoserine; by PKCBy similarity | 1 | |
Modified residuei | 563 | Symmetric dimethylarginine; by PRMT5By similarity | 1 | |
Modified residuei | 621 | Phosphoserine1 Publication | 1 | |
Modified residuei | 642 | Phosphoserine; by MAPK1Combined sources1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Methylation, PhosphoproteinProteomic databases
EPDi | Q99N57 |
jPOSTi | Q99N57 |
MaxQBi | Q99N57 |
PaxDbi | Q99N57 |
PeptideAtlasi | Q99N57 |
PRIDEi | Q99N57 |
ProteomicsDBi | 254892 [Q99N57-1] 254893 [Q99N57-2] |
PTM databases
iPTMneti | Q99N57 |
PhosphoSitePlusi | Q99N57 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000000441, Expressed in granulocyte and 326 other tissues |
ExpressionAtlasi | Q99N57, baseline and differential |
Genevisiblei | Q99N57, MM |
Interactioni
Subunit structurei
Monomer (By similarity). Homodimer (By similarity). Heterodimerizes with BRAF and this heterodimer possesses a highly increased kinase activity compared to the respective homodimers or monomers (By similarity). Heterodimerization is mitogen-regulated and enhanced by 14-3-3 proteins (By similarity). MAPK1/ERK2 activation can induce a negative feedback that promotes the dissociation of the heterodimer (By similarity).
Forms a multiprotein complex with Ras (M-Ras/MRAS), SHOC2 and protein phosphatase 1 (PPP1CA, PPP1CB and PPP1CC) (By similarity).
Interacts with LZTR1 (By similarity).
Interacts with Ras proteins; the interaction is antagonized by RIN1 (By similarity). Weakly interacts with RIT1 (By similarity).
Interacts (via N-terminus) with RGS14 (via RBD domains); the interaction mediates the formation of a ternary complex with BRAF, a ternary complex inhibited by GNAI1 (By similarity). Probably forms a complex composed of chaperones HSP90 and HSP70, co-chaperones CDC37, PPP5C, TSC1 and client protein TSC2, CDK4, AKT, RAF1 and NR3C1; this complex does not contain co-chaperones STIP1/HOP and PTGES3/p23 (By similarity).
Interacts with STK3/MST2; the interaction inhibits its pro-apoptotic activity (By similarity).
Interacts (when phosphorylated at Ser-259) with YWHAZ (unphosphorylated at 'Thr-232') (By similarity).
Interacts with MAP2K1/MEK1 and MAP2K2/MEK2 (By similarity).
Interacts with MAP3K5/ASF1 (via N-terminus) and this interaction inhibits the proapoptotic function of MAP3K5/ASK1 (By similarity).
Interacts with PAK1 (via kinase domain) (By similarity). The Ser-338 and Ser-339 phosphorylated form (by PAK1) interacts with BCL2 (By similarity).
Interacts with PEBP1/RKIP and this interaction is enhanced if RAF1 is phosphorylated on residues Ser-338, Ser-339, Tyr-340 and Tyr-341 (By similarity).
Interacts with ADCY2, ADCY5, ADCY6, DGKH, RCAN1/DSCR1, PPP1R12A, PKB/AKT1, SPRY2, SPRY4, CNKSR1/CNK1, KSR2 and PHB/prohibitin (By similarity). The phosphorylated form interacts with PIN1 (PubMed:15664191).
Interacts with PPP2CA, PPP2R1B and ROCK2 (PubMed:15753127, PubMed:15664191). In its active form, interacts with PRMT5 (By similarity).
Interacts with FAM83B; displaces 14-3-3 proteins from RAF1 and activates RAF1 (By similarity).
Interacts with PDE8A; the interaction promotes RAF1 activity (By similarity).
Interacts with MFHAS1 (By similarity).
Interacts with GLS (By similarity).
Interacts with NEK10 and MAP2K1; the interaction is direct with NEK10 and required for ERK1/2-signaling pathway activation in response to UV irradiation (By similarity).
By similarity2 PublicationsBinary interactionsi
Q99N57
GO - Molecular functioni
- adenylate cyclase binding Source: MGI
- enzyme binding Source: MGI
- identical protein binding Source: MGI
- mitogen-activated protein kinase kinase binding Source: MGI
- small GTPase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 225343, 17 interactors |
CORUMi | Q99N57 |
DIPi | DIP-31555N |
IntActi | Q99N57, 15 interactors |
MINTi | Q99N57 |
STRINGi | 10090.ENSMUSP00000000451 |
Chemistry databases
BindingDBi | Q99N57 |
Miscellaneous databases
RNActi | Q99N57, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 56 – 131 | RBDPROSITE-ProRule annotationAdd BLAST | 76 | |
Domaini | 349 – 609 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 261 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 205 – 265 | DisorderedSequence analysisAdd BLAST | 61 | |
Regioni | 281 – 335 | DisorderedSequence analysisAdd BLAST | 55 | |
Regioni | 331 – 349 | Interaction with PEBP1/RKIPBy similarityAdd BLAST | 19 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 222 – 265 | Polar residuesSequence analysisAdd BLAST | 44 | |
Compositional biasi | 284 – 310 | Polar residuesSequence analysisAdd BLAST | 27 |
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 138 – 184 | Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST | 47 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG0193, Eukaryota |
GeneTreei | ENSGT00940000156084 |
HOGENOMi | CLU_023684_1_1_1 |
InParanoidi | Q99N57 |
OMAi | SWCHRFW |
OrthoDBi | 243095at2759 |
PhylomeDBi | Q99N57 |
TreeFami | TF317006 |
Family and domain databases
CDDi | cd00029, C1, 1 hit |
InterProi | View protein in InterPro IPR046349, C1-like_sf IPR020454, DAG/PE-bd IPR011009, Kinase-like_dom_sf IPR002219, PE/DAG-bd IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR003116, RBD_dom IPR001245, Ser-Thr/Tyr_kinase_cat_dom IPR008271, Ser/Thr_kinase_AS IPR029071, Ubiquitin-like_domsf |
Pfami | View protein in Pfam PF00130, C1_1, 1 hit PF07714, PK_Tyr_Ser-Thr, 1 hit PF02196, RBD, 1 hit |
PRINTSi | PR00008, DAGPEDOMAIN |
SMARTi | View protein in SMART SM00109, C1, 1 hit SM00455, RBD, 1 hit SM00220, S_TKc, 1 hit |
SUPFAMi | SSF54236, SSF54236, 1 hit SSF56112, SSF56112, 1 hit SSF57889, SSF57889, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit PS50898, RBD, 1 hit PS00479, ZF_DAG_PE_1, 1 hit PS50081, ZF_DAG_PE_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEHIQGAWKT ISNGFGLKDA VFDGSSCISP TIVQQFGYQR RASDDGKLTD
60 70 80 90 100
SSKTSNTIRV FLPNKQRTVV NVRNGMSLHD CLMKALKVRG LQPECCAVFR
110 120 130 140 150
LLQEHKGKKA RLDWNTDAAS LIGEELQVDF LDHVPLTTHN FARKTFLKLA
160 170 180 190 200
FCDICQKFLL NGFRCQTCGY KFHEHCSTKV PTMCVDWSNI RQLLLFPNST
210 220 230 240 250
VGDSGVPAPP SFPMRRMRES VSRMPASSQH RYSTPHAFTF NTSSPSSEGS
260 270 280 290 300
LSQRQRSTST PNVHMVSTTL HVDSRMIEDA IRSHSESASP SALSSSPNNL
310 320 330 340 350
SPTGWSQPKT PVPAQRERAP GSGTQEKNKI RPRGQRDSSY YWEIEASEVM
360 370 380 390 400
LSTRIGSGSF GTVYKGKWHG DVAVKILKVV DPTPEQLQAF RNEVAVLRKT
410 420 430 440 450
RHVNILLFMG YMTKDNLAIV TQWCEGSSLY KHLHVQETKF QMFQLIDIAR
460 470 480 490 500
QTAQGMDYLH AKNIIHRDMK SNNIFLHEGL TVKIGDFGLA TVKSRWSGSQ
510 520 530 540 550
QVEQPTGSVL WMAPEVIRMQ DDNPFSFQSD VYSYGIVLYE LMAGELPYAH
560 570 580 590 600
INNRDQIIFM VGRGYASPDL SRLYKNCPKA MKRLVADCVK KVKEERPLFP
610 620 630 640
QILSSIELLQ HSLPKINRSA SEPSLHRAAH TEDINACTLT TSPRLPVF
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF6TUC4 | F6TUC4_MOUSE | RAF proto-oncogene serine/threonine... | Raf1 | 205 | Annotation score: | ||
A0A0N4SWH5 | A0A0N4SWH5_MOUSE | RAF proto-oncogene serine/threonine... | Raf1 | 96 | Annotation score: | ||
A0A0N4SV36 | A0A0N4SV36_MOUSE | RAF proto-oncogene serine/threonine... | Raf1 | 118 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 522 | D → N in AAH92040 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 543 | A → T in AAH92040 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_034629 | 278 | E → ESNSLNASPRACSRRFCLRG R in isoform 2. Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB057655 mRNA Translation: BAB39748.1 AB057663 mRNA Translation: BAB39743.2 AK141745 mRNA Translation: BAE24820.1 BC015273 mRNA Translation: AAH15273.1 BC092040 mRNA Translation: AAH92040.1 X55432 Genomic DNA No translation available. |
CCDSi | CCDS20441.1 [Q99N57-1] |
RefSeqi | NP_084056.1, NM_029780.3 [Q99N57-1] XP_006505426.1, XM_006505363.3 XP_006505427.1, XM_006505364.2 [Q99N57-2] XP_006505428.1, XM_006505365.2 |
Genome annotation databases
Ensembli | ENSMUST00000000451; ENSMUSP00000000451; ENSMUSG00000000441 [Q99N57-1] ENSMUST00000112949; ENSMUSP00000108571; ENSMUSG00000000441 [Q99N57-1] |
GeneIDi | 110157 |
KEGGi | mmu:110157 |
UCSCi | uc009dix.1, mouse [Q99N57-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB057655 mRNA Translation: BAB39748.1 AB057663 mRNA Translation: BAB39743.2 AK141745 mRNA Translation: BAE24820.1 BC015273 mRNA Translation: AAH15273.1 BC092040 mRNA Translation: AAH92040.1 X55432 Genomic DNA No translation available. |
CCDSi | CCDS20441.1 [Q99N57-1] |
RefSeqi | NP_084056.1, NM_029780.3 [Q99N57-1] XP_006505426.1, XM_006505363.3 XP_006505427.1, XM_006505364.2 [Q99N57-2] XP_006505428.1, XM_006505365.2 |
3D structure databases
AlphaFoldDBi | Q99N57 |
BMRBi | Q99N57 |
SMRi | Q99N57 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 225343, 17 interactors |
CORUMi | Q99N57 |
DIPi | DIP-31555N |
IntActi | Q99N57, 15 interactors |
MINTi | Q99N57 |
STRINGi | 10090.ENSMUSP00000000451 |
Chemistry databases
BindingDBi | Q99N57 |
ChEMBLi | CHEMBL3804748 |
PTM databases
iPTMneti | Q99N57 |
PhosphoSitePlusi | Q99N57 |
Proteomic databases
EPDi | Q99N57 |
jPOSTi | Q99N57 |
MaxQBi | Q99N57 |
PaxDbi | Q99N57 |
PeptideAtlasi | Q99N57 |
PRIDEi | Q99N57 |
ProteomicsDBi | 254892 [Q99N57-1] 254893 [Q99N57-2] |
Protocols and materials databases
Antibodypediai | 1131, 3158 antibodies from 49 providers |
DNASUi | 110157 |
Genome annotation databases
Ensembli | ENSMUST00000000451; ENSMUSP00000000451; ENSMUSG00000000441 [Q99N57-1] ENSMUST00000112949; ENSMUSP00000108571; ENSMUSG00000000441 [Q99N57-1] |
GeneIDi | 110157 |
KEGGi | mmu:110157 |
UCSCi | uc009dix.1, mouse [Q99N57-1] |
Organism-specific databases
CTDi | 5894 |
MGIi | MGI:97847, Raf1 |
VEuPathDBi | HostDB:ENSMUSG00000000441 |
Phylogenomic databases
eggNOGi | KOG0193, Eukaryota |
GeneTreei | ENSGT00940000156084 |
HOGENOMi | CLU_023684_1_1_1 |
InParanoidi | Q99N57 |
OMAi | SWCHRFW |
OrthoDBi | 243095at2759 |
PhylomeDBi | Q99N57 |
TreeFami | TF317006 |
Enzyme and pathway databases
Reactomei | R-MMU-2672351, Stimuli-sensing channels R-MMU-392517, Rap1 signalling R-MMU-430116, GP1b-IX-V activation signalling R-MMU-5621575, CD209 (DC-SIGN) signaling R-MMU-5673000, RAF activation R-MMU-5674135, MAP2K and MAPK activation R-MMU-5674499, Negative feedback regulation of MAPK pathway R-MMU-5675221, Negative regulation of MAPK pathway |
Miscellaneous databases
BioGRID-ORCSi | 110157, 10 hits in 75 CRISPR screens |
ChiTaRSi | Raf1, mouse |
PROi | PR:Q99N57 |
RNActi | Q99N57, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000000441, Expressed in granulocyte and 326 other tissues |
ExpressionAtlasi | Q99N57, baseline and differential |
Genevisiblei | Q99N57, MM |
Family and domain databases
CDDi | cd00029, C1, 1 hit |
InterProi | View protein in InterPro IPR046349, C1-like_sf IPR020454, DAG/PE-bd IPR011009, Kinase-like_dom_sf IPR002219, PE/DAG-bd IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR003116, RBD_dom IPR001245, Ser-Thr/Tyr_kinase_cat_dom IPR008271, Ser/Thr_kinase_AS IPR029071, Ubiquitin-like_domsf |
Pfami | View protein in Pfam PF00130, C1_1, 1 hit PF07714, PK_Tyr_Ser-Thr, 1 hit PF02196, RBD, 1 hit |
PRINTSi | PR00008, DAGPEDOMAIN |
SMARTi | View protein in SMART SM00109, C1, 1 hit SM00455, RBD, 1 hit SM00220, S_TKc, 1 hit |
SUPFAMi | SSF54236, SSF54236, 1 hit SSF56112, SSF56112, 1 hit SSF57889, SSF57889, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit PS50898, RBD, 1 hit PS00479, ZF_DAG_PE_1, 1 hit PS50081, ZF_DAG_PE_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RAF1_MOUSE | |
Accessioni | Q99N57Primary (citable) accession number: Q99N57 Secondary accession number(s): Q3UR68 Q9QUU8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 15, 2003 |
Last sequence update: | March 1, 2002 | |
Last modified: | May 25, 2022 | |
This is version 193 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human and mouse protein kinases
Human and mouse protein kinases: classification and index - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families