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Protein

Sorting nexin-1

Gene

Snx1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in several stages of intracellular trafficking. Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Acts in part as component of the retromer membrane-deforming SNX-BAR subcomplex. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX-BAR subcomplex functions to deform the donor membrane into a tubular profile called endosome-to-TGN transport carrier (ETC). Can sense membrane curvature and has in vitro vesicle-to-membrane remodeling activity. Involved in retrograde endosome-to-TGN transport of lysosomal enzyme receptors (IGF2R, M6PR and SORT1). Plays a role in targeting ligand-activated EGFR to the lysosomes for degradation after endocytosis from the cell surface and release from the Golgi. Involvement in retromer-independent endocytic trafficking of P2RY1 and lysosomal degradation of protease-activated receptor-1/F2R. Promotes KALRN- and RHOG-dependent but retromer-independent membrane remodeling such as lamellipodium formation; the function is dependent on GEF activity of KALRN. Required for endocytosis of DRD5 upon agonist stimulation but not for basal receptor trafficking (By similarity).By similarity

Miscellaneous

Binds phosphatidylinositol 3-phosphate (PtdIns-3P) and phosphatidylinositol 3,5-bisphosphate (PtdIns-(3,5)P2) in liposome-based assays. Can bind PtdIns(3,4,5)P3 in protein:lipid overlay assays, but not in liposome-based assays (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei186Phosphatidylinositol 3-phosphateBy similarity1
Binding sitei188Phosphatidylinositol 3-phosphate; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei214Phosphatidylinositol 3-phosphateBy similarity1
Binding sitei238Phosphatidylinositol 3-phosphateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport
LigandLipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Sorting nexin-1
Gene namesi
Name:Snx1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi68935 Snx1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Endosome, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002138371 – 522Sorting nexin-1Add BLAST522

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei32PhosphoserineCombined sources1
Modified residuei39PhosphoserineBy similarity1
Modified residuei41PhosphothreonineBy similarity1
Modified residuei48PhosphothreonineBy similarity1
Modified residuei58PhosphoserineBy similarity1
Modified residuei72PhosphoserineBy similarity1
Modified residuei188PhosphoserineCombined sources1
Modified residuei237N6-acetyllysineBy similarity1
Modified residuei280PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ99N27
PRIDEiQ99N27

PTM databases

iPTMnetiQ99N27
PhosphoSitePlusiQ99N27

Expressioni

Gene expression databases

BgeeiENSRNOG00000017029 Expressed in 9 organ(s), highest expression level in spleen
GenevisibleiQ99N27 RN

Interactioni

Subunit structurei

Predominantly forms heterodimers with BAR domain-containing sorting nexins SNX5, SNX6 and SNX32; can self-associate to form homodimers. The heterodimers are proposed to self-assemble into helical arrays on the membrane to stabilize and expand local membrane curvature underlying endosomal tubule formation. Thought to be a component of the originally described retromer complex (also called SNX-BAR retromer) which is a pentamer containing the heterotrimeric retromer cargo-selective complex (CSC), also described as vacuolar protein sorting subcomplex (VPS) and a heterodimeric membrane-deforming subcomplex formed between SNX1 or SNX2 and SNX5 or SNX6 (also called SNX-BAR subcomplex); the respective CSC and SNX-BAR subcomplexes associate with low affinity. Interacts with SNX5, SNX6, SNX32, VPS26A, VPS29, VPS35, DRD5, DENND5A, KALRN, RHOG (GDP-bound form). The interaction with SNX2 is reported controversially. Interacts with DNAJC13; prevented by presence of HGS (By similarity). Interacts with HGS (PubMed:11110793).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ99N27, 1 interactor
STRINGi10116.ENSRNOP00000022960

Structurei

3D structure databases

ProteinModelPortaliQ99N27
SMRiQ99N27
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini143 – 272PXPROSITE-ProRule annotationAdd BLAST130
Domaini302 – 522BARAdd BLAST221

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni281 – 298Membrane-binding amphipathic helixBy similarityAdd BLAST18

Domaini

The BAR domain is able to sense membrane curvature upon dimerization. Membrane remodeling seems to implicate insertion of a N-terminal amphipatric helix (AH) in the membrane (By similarity).By similarity

Sequence similaritiesi

Belongs to the sorting nexin family.Curated

Phylogenomic databases

eggNOGiKOG2273 Eukaryota
COG5391 LUCA
GeneTreeiENSGT00910000144062
HOGENOMiHOG000293327
HOVERGENiHBG000618
InParanoidiQ99N27
KOiK17917
OMAiFLMQLDA
OrthoDBiEOG091G06FB
PhylomeDBiQ99N27
TreeFamiTF313698

Family and domain databases

CDDicd07281 PX_SNX1, 1 hit
Gene3Di1.20.1270.60, 1 hit
3.30.1520.10, 1 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR001683 Phox
IPR036871 PX_dom_sf
IPR034901 PX_SNX1
IPR028660 SNX1
IPR039358 Sorting_nexin-like
IPR005329 Sorting_nexin_N
IPR015404 Vps5_C
PANTHERiPTHR10555 PTHR10555, 1 hit
PTHR10555:SF129 PTHR10555:SF129, 1 hit
PfamiView protein in Pfam
PF00787 PX, 1 hit
PF03700 Sorting_nexin, 1 hit
PF09325 Vps5, 1 hit
SMARTiView protein in SMART
SM00312 PX, 1 hit
SUPFAMiSSF103657 SSF103657, 1 hit
SSF64268 SSF64268, 1 hit
PROSITEiView protein in PROSITE
PS50195 PX, 1 hit

Sequencei

Sequence statusi: Complete.

Q99N27-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MASGGGGCSA SERLPPPFPG MDPESEGAAG GSEPEAGDSD TEGEDIFTGA
60 70 80 90 100
AAASKPQSPK KTTSLFPIKN GSKENGIHEE QDQEPQDLFA DATVELSLDS
110 120 130 140 150
TQNNQKTMPG KTLIPHPTQE ATNSPKPQPS YEELEEEEQE DQFDLTVGIT
160 170 180 190 200
DPEKIGDGMN AYVAYKVTTQ TSLPMFRSRQ FAVKRRFSDF LGLYEKLSEK
210 220 230 240 250
HSQNGFIVPP PPEKSLIGMT KVKVGKEDSS SAEFLEKRRA ALERYLQRIV
260 270 280 290 300
NHPTMLQDPD VREFLEKEEL PRAVGTQALS GAGLLKMFNK ATDAVSKMTI
310 320 330 340 350
KMNESDIWFE EKLQEVECEE QRLRKLHAVV ETLVNHRKEL ALNTALFAKS
360 370 380 390 400
LAMLGSSEDN TALSRALSQL AEVEEKIEQL HQEQANNDFF LLAELLSDYI
410 420 430 440 450
RLLAIVRAAF DQRMKTWQRW QDAQATLQKK RESEARLLWA NKPDKLQQAK
460 470 480 490 500
DEITEWESRV TQYERDFERI STVVRKEVTR FEKEKSKDFK NHVIKYLETL
510 520
LHSQQQLAKY WEAFLPEARA IS
Length:522
Mass (Da):59,044
Last modified:June 1, 2001 - v1
Checksum:iF0F05664087E4D24
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF218916 mRNA Translation: AAG59616.1
BC092201 mRNA Translation: AAH92201.1
RefSeqiNP_445863.1, NM_053411.1
UniGeneiRn.144614

Genome annotation databases

EnsembliENSRNOT00000022960; ENSRNOP00000022960; ENSRNOG00000017029
GeneIDi84471
KEGGirno:84471
UCSCiRGD:68935 rat

Similar proteinsi

Entry informationi

Entry nameiSNX1_RAT
AccessioniPrimary (citable) accession number: Q99N27
Secondary accession number(s): Q56A25
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: June 1, 2001
Last modified: September 12, 2018
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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