UniProtKB - Q99N16 (CP4F3_MOUSE)
Cytochrome P450 4F3
Cyp4f3
Functioni
A cytochrome P450 monooxygenase involved in the metabolism of the proinflammatory lipid mediator leukotriene B4 (LTB4) (PubMed:16380383, PubMed:24632148).
Hydroxylates at the omega-1 and omega-2 positions LTB4. This oxidation step leads to LTB4 inactivation, which is postulated to be a crucial part of the resolution of inflammation (PubMed:16380383, PubMed:24632148).
Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:16380383, PubMed:24632148).
2 PublicationsCatalytic activityi
- leukotriene B4 + O2 + reduced [NADPH—hemoprotein reductase] = 18-hydroxy-leukotriene B4 + H+ + H2O + oxidized [NADPH—hemoprotein reductase]2 PublicationsThis reaction proceeds in the forward2 Publications direction.
- leukotriene B4 + O2 + reduced [NADPH—hemoprotein reductase] = 19-hydroxy-leukotriene B4 + H+ + H2O + oxidized [NADPH—hemoprotein reductase]2 PublicationsThis reaction proceeds in the forward2 Publications direction.
Cofactori
: leukotriene B4 degradation Pathwayi
This protein is involved in the pathway leukotriene B4 degradation, which is part of Lipid metabolism.2 PublicationsView all proteins of this organism that are known to be involved in the pathway leukotriene B4 degradation and in Lipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 468 | Iron (heme axial ligand)By similarity | 1 |
GO - Molecular functioni
- arachidonic acid monooxygenase activity Source: GO_Central
- docosanoate omega-hydroxylase activity Source: MGI
- heme binding Source: InterPro
- iron ion binding Source: InterPro
- leukotriene-B4 20-monooxygenase activity Source: MGI
- long-chain fatty acid omega-1 hydroxylase activity Source: UniProtKB
- long-chain fatty acid omega-hydroxylase activity Source: MGI
GO - Biological processi
- arachidonic acid metabolic process Source: GO_Central
- fatty acid omega-oxidation Source: MGI
- leukotriene B4 catabolic process Source: MGI
- leukotriene B4 metabolic process Source: UniProtKB
- menaquinone catabolic process Source: GO_Central
- phylloquinone catabolic process Source: GO_Central
- vitamin K catabolic process Source: GO_Central
Keywordsi
Molecular function | Monooxygenase, Oxidoreductase |
Biological process | Lipid metabolism |
Ligand | Heme, Iron, Metal-binding |
Enzyme and pathway databases
Reactomei | R-MMU-211935, Fatty acids R-MMU-211958, Miscellaneous substrates R-MMU-211979, Eicosanoids R-MMU-2142691, Synthesis of Leukotrienes (LT) and Eoxins (EX) R-MMU-2142816, Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE) |
UniPathwayi | UPA00883 |
Chemistry databases
SwissLipidsi | SLP:000001724 |
Names & Taxonomyi
Protein namesi | Recommended name: Cytochrome P450 4F31 PublicationAlternative name(s): CYPIVF3 Leukotriene-B(4) omega-1/omega-2 hydroxylase1 Publication |
Gene namesi | Name:Cyp4f3By similarity Synonyms:Cyp4f181 PublicationImported |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1919304, Cyp4f18 |
VEuPathDBi | HostDB:ENSMUSG00000003484 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication; Single-pass membrane protein Curated
- Microsome membrane 1 Publication; Single-pass membrane protein Curated
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
- intracellular membrane-bounded organelle Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 19 – 39 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Endoplasmic reticulum, Membrane, MicrosomePathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000238924 | 1 – 524 | Cytochrome P450 4F3Add BLAST | 524 |
Proteomic databases
jPOSTi | Q99N16 |
MaxQBi | Q99N16 |
PaxDbi | Q99N16 |
PeptideAtlasi | Q99N16 |
PRIDEi | Q99N16 |
ProteomicsDBi | 285281 |
PTM databases
iPTMneti | Q99N16 |
PhosphoSitePlusi | Q99N16 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000003484, Expressed in granulocyte and 104 other tissues |
ExpressionAtlasi | Q99N16, baseline and differential |
Genevisiblei | Q99N16, MM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 215120, 29 interactors |
STRINGi | 10090.ENSMUSP00000003574 |
Miscellaneous databases
RNActi | Q99N16, protein |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0157, Eukaryota |
GeneTreei | ENSGT00940000163407 |
HOGENOMi | CLU_001570_5_1_1 |
InParanoidi | Q99N16 |
OMAi | TFTTMCI |
OrthoDBi | 1247045at2759 |
PhylomeDBi | Q99N16 |
TreeFami | TF105088 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MSQLSMSWMG LGHTAASPWL LLLLAGASCL LAYILTPIYG VFENSLRLRC
60 70 80 90 100
FPQPPKRNWI LGHLGLIQSS EEGLLYIQSL VRTFRDACCW WVGPLHPVIR
110 120 130 140 150
IFHPAFIKPV VLAPALVAPK DTVFYRFLKP WLGDGLLMST GDKWSRHRRM
160 170 180 190 200
LTPAFHFNIL KPYVKVFNDS TNIMHAKWQR LASKGSAYLN MFEHISLMTL
210 220 230 240 250
DSLQKCVFSF DSNCQEKPSE YITAILELST LVARRHQRLL LHVDLFYYLT
260 270 280 290 300
HDGMRFRKAC RLVHDFTDAV IRERRRTLLD QGGVDVLKAK AKAKTLDFID
310 320 330 340 350
VLLLSKDEHG KALSDEDIRA EADTFMFGGH DTTASGLSWI LYNLARHPEY
360 370 380 390 400
QERCRQEVRE LLRDREPEEI EWDDLAQLPF LTMCIKESLR LHPPVTAISR
410 420 430 440 450
CCTQDIVLPD GRVIPKGVIS RISIFGTHHN PAVWPDPEVY DPFRFDADNV
460 470 480 490 500
KGRSPLAFIP FSAGPRNCIG QTFAMSEMKV ALALTLLRFR VLPDDKEPRR
510 520
KPELILRAEG GLWLKVEPLS AGAQ
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A1D5RLD0 | A0A1D5RLD0_MOUSE | Cytochrome P450, family 4, subfamil... | Cyp4f18 | 175 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 6 | M → L in AAK15013 (Ref. 1) Curated | 1 | |
Sequence conflicti | 6 | M → L in AAH13494 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 37 | P → Q in AAK15013 (Ref. 1) Curated | 1 | |
Sequence conflicti | 37 | P → Q in AAH13494 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 142 | D → G in BAB25315 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 230 | T → A in AAK15013 (Ref. 1) Curated | 1 | |
Sequence conflicti | 230 | T → A in AAH13494 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 284 | V → D in AAK15013 (Ref. 1) Curated | 1 | |
Sequence conflicti | 284 | V → D in AAH13494 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF233647 mRNA Translation: AAK15013.1 AK007863 mRNA Translation: BAB25315.1 AC162522 Genomic DNA No translation available. BC013494 mRNA Translation: AAH13494.1 |
CCDSi | CCDS22406.1 |
RefSeqi | NP_077764.2, NM_024444.2 XP_006509811.1, XM_006509748.2 |
Genome annotation databases
Ensembli | ENSMUST00000003574; ENSMUSP00000003574; ENSMUSG00000003484 |
GeneIDi | 72054 |
KEGGi | mmu:72054 |
UCSCi | uc009mfe.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF233647 mRNA Translation: AAK15013.1 AK007863 mRNA Translation: BAB25315.1 AC162522 Genomic DNA No translation available. BC013494 mRNA Translation: AAH13494.1 |
CCDSi | CCDS22406.1 |
RefSeqi | NP_077764.2, NM_024444.2 XP_006509811.1, XM_006509748.2 |
3D structure databases
SMRi | Q99N16 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 215120, 29 interactors |
STRINGi | 10090.ENSMUSP00000003574 |
Chemistry databases
SwissLipidsi | SLP:000001724 |
PTM databases
iPTMneti | Q99N16 |
PhosphoSitePlusi | Q99N16 |
Proteomic databases
jPOSTi | Q99N16 |
MaxQBi | Q99N16 |
PaxDbi | Q99N16 |
PeptideAtlasi | Q99N16 |
PRIDEi | Q99N16 |
ProteomicsDBi | 285281 |
Protocols and materials databases
Antibodypediai | 27108, 139 antibodies from 27 providers |
Genome annotation databases
Ensembli | ENSMUST00000003574; ENSMUSP00000003574; ENSMUSG00000003484 |
GeneIDi | 72054 |
KEGGi | mmu:72054 |
UCSCi | uc009mfe.2, mouse |
Organism-specific databases
CTDi | 72054 |
MGIi | MGI:1919304, Cyp4f18 |
VEuPathDBi | HostDB:ENSMUSG00000003484 |
Phylogenomic databases
eggNOGi | KOG0157, Eukaryota |
GeneTreei | ENSGT00940000163407 |
HOGENOMi | CLU_001570_5_1_1 |
InParanoidi | Q99N16 |
OMAi | TFTTMCI |
OrthoDBi | 1247045at2759 |
PhylomeDBi | Q99N16 |
TreeFami | TF105088 |
Enzyme and pathway databases
UniPathwayi | UPA00883 |
Reactomei | R-MMU-211935, Fatty acids R-MMU-211958, Miscellaneous substrates R-MMU-211979, Eicosanoids R-MMU-2142691, Synthesis of Leukotrienes (LT) and Eoxins (EX) R-MMU-2142816, Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE) |
Miscellaneous databases
BioGRID-ORCSi | 72054, 2 hits in 66 CRISPR screens |
PROi | PR:Q99N16 |
RNActi | Q99N16, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000003484, Expressed in granulocyte and 104 other tissues |
ExpressionAtlasi | Q99N16, baseline and differential |
Genevisiblei | Q99N16, MM |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CP4F3_MOUSE | |
Accessioni | Q99N16Primary (citable) accession number: Q99N16 Secondary accession number(s): E9QLZ3, Q9D8N4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2006 |
Last sequence update: | July 27, 2011 | |
Last modified: | February 23, 2022 | |
This is version 149 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families