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Protein

Carbohydrate-responsive element-binding protein

Gene

Mlxipl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional repressor. Binds to the canonical and non-canonical E box sequences 5'-CACGTG-3'.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-163358 PKA-mediated phosphorylation of key metabolic factors
R-MMU-163765 ChREBP activates metabolic gene expression

Names & Taxonomyi

Protein namesi
Recommended name:
Carbohydrate-responsive element-binding protein
Short name:
ChREBP
Alternative name(s):
MLX interactor
MLX-interacting protein-like
Williams-Beuren syndrome chromosomal region 14 protein homolog
Gene namesi
Name:Mlxipl
Synonyms:Mio, Wbscr14
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1927999 Mlxipl

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001275051 – 864Carbohydrate-responsive element-binding proteinAdd BLAST864

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei20PhosphoserineCombined sources1
Modified residuei23PhosphoserineCombined sources1
Modified residuei25PhosphoserineCombined sources1
Modified residuei27PhosphothreonineCombined sources1
Modified residuei196PhosphoserineBy similarity1
Modified residuei566Phosphoserine; by AMPKBy similarity1
Modified residuei614PhosphoserineBy similarity1
Modified residuei626PhosphoserineCombined sources1
Modified residuei643PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylation at Ser-566 by AMPK inactivates the DNA-binding activity.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ99MZ3
PeptideAtlasiQ99MZ3
PRIDEiQ99MZ3

PTM databases

iPTMnetiQ99MZ3
PhosphoSitePlusiQ99MZ3

Expressioni

Tissue specificityi

Expressed in the ventricular and intermediate zones of the developing spinal cord of E12.5 embryos. In later embryos expressed in a variety of tissues.

Gene expression databases

BgeeiENSMUSG00000005373 Expressed in 131 organ(s), highest expression level in brown adipose tissue
CleanExiMM_MLXIPL
ExpressionAtlasiQ99MZ3 baseline and differential
GenevisibleiQ99MZ3 MM

Interactioni

Subunit structurei

Binds DNA as a heterodimer with TCFL4/MLX.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi208443, 6 interactors
IntActiQ99MZ3, 1 interactor
MINTiQ99MZ3
STRINGi10090.ENSMUSP00000005507

Structurei

Secondary structure

1864
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ99MZ3
SMRiQ99MZ3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini661 – 715bHLHPROSITE-ProRule annotationAdd BLAST55

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni715 – 736Leucine-zipperAdd BLAST22

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi345 – 350Poly-Ser6

Phylogenomic databases

eggNOGiKOG3582 Eukaryota
ENOG410XTA5 LUCA
GeneTreeiENSGT00530000063219
HOGENOMiHOG000113606
HOVERGENiHBG073589
InParanoidiQ99MZ3
KOiK09113
OMAiTAAKPEQ
OrthoDBiEOG091G02A1
PhylomeDBiQ99MZ3
TreeFamiTF324749

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
PfamiView protein in Pfam
PF00010 HLH, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit

Sequences (5+)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 5 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q99MZ3-1) [UniParc]FASTAAdd to basket
Also known as: Zeta

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MARALADLSV NLQVPRVVPS PDSDSDTDLE DPSPRRSAGG LHRSQVIHSG
60 70 80 90 100
HFMVSSPHSD SLTRRRDQEG PVGLADFGPR SIDPTLTHLF ECLSLAYSGK
110 120 130 140 150
LVSPKWKNFK GLKLLCRDKI RLNNAIWRAW YIQYVQRRKS PVCGFVTPLQ
160 170 180 190 200
GSEADEHRKP EAVILEGNYW KRRIEVVMRE YHKWRIYYKK RLRKSSREGD
210 220 230 240 250
FLAPKQVEGG WPPPERWCEQ LFSSVVPVLL GGSEEEPGGR QLLDLDCFLS
260 270 280 290 300
DISDTLFTMT QPSPSSLQLP PEDAYVGNAD MIQPDLTPLQ PSLDDFMEIS
310 320 330 340 350
DFFTNYRPPQ TPTSSNYIES PSFGPMADSL FSSGILAPEM PSPASSSSSS
360 370 380 390 400
GMTPHSGNTR LQARNSCSGP LDPNPFLSSE FLLPEDPKTK IPPAPGPTPL
410 420 430 440 450
LPFPTPVKVH GLEPCTPSPF PTMAPPPSLL PEESLLSARF PFTSAPPAPG
460 470 480 490 500
VSTLPAPTTF VPTPQPGPGP VPFSVDHLPH GYLEPVFGPH FTVPQGMQPR
510 520 530 540 550
CKPSSPSPGG QKASPPTLAS ATASPTATAT ARDNNPCLTQ LLRAAKPEQA
560 570 580 590 600
LEPPTMPGTL LRPPESPQDT VSEIPRARAF FPPIPAPTPP RPPPGPATLA
610 620 630 640 650
PPRSLVVPKA ERLSPPASSG SERRLSGDLN SIQPSGALSV HLSPPQTVLS
660 670 680 690 700
RGRVDNNKME NRRITHISAE QKRRFNIKLG FDTLHGLVST LSAQPSLKVS
710 720 730 740 750
KATTLQKTAE YILMLQQERA AMQEEAQQLR DEIEELNAAI NLCQQQLPAT
760 770 780 790 800
GVPITHQRFD QMRDMFDDYV RTRTLHNWKF WVFSILIRPL FESFNGMVST
810 820 830 840 850
ASLHSLRQTS LAWLEQYCSL PALRPTVLNS LRQLSTSTSI LTDPSLVPEQ
860
ATRAVTEGTL GRPL
Length:864
Mass (Da):94,875
Last modified:June 1, 2001 - v1
Checksum:i7E6AFFB04C71B327
GO
Isoform 2 (identifier: Q99MZ3-2) [UniParc]FASTAAdd to basket
Also known as: Theta

The sequence of this isoform differs from the canonical sequence as follows:
     58-79: Missing.

Show »
Length:842
Mass (Da):92,482
Checksum:i14BFA5F3820AFF74
GO
Isoform 3 (identifier: Q99MZ3-3) [UniParc]FASTAAdd to basket
Also known as: Iota

The sequence of this isoform differs from the canonical sequence as follows:
     699-744: VSKATTLQKT...ELNAAINLCQ → GLPTQRPTLV...YAGGGAAAAG
     745-864: Missing.

Show »
Length:744
Mass (Da):80,170
Checksum:iF052B12D7CE0259B
GO
Isoform 4 (identifier: Q99MZ3-4) [UniParc]FASTAAdd to basket
Also known as: Kappa

The sequence of this isoform differs from the canonical sequence as follows:
     699-714: VSKATTLQKTAEYILM → LPGLANTEAHIGGARR
     715-864: Missing.

Show »
Length:714
Mass (Da):77,556
Checksum:i4BAD0BE936A72292
GO
Isoform 5 (identifier: Q99MZ3-5) [UniParc]FASTAAdd to basket
Also known as: Eta

The sequence of this isoform differs from the canonical sequence as follows:
     545-556: AKPEQALEPPTM → VVLIVLPVPSQA
     557-864: Missing.

Show »
Length:556
Mass (Da):60,602
Checksum:iBF8BA1D9BEA7E942
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F6Q4B6F6Q4B6_MOUSE
Carbohydrate-responsive element-bin...
Mlxipl
179Annotation score:
F6Q3B6F6Q3B6_MOUSE
Carbohydrate-responsive element-bin...
Mlxipl
181Annotation score:
A0A0J9YUT0A0A0J9YUT0_MOUSE
Carbohydrate-responsive element-bin...
Mlxipl
545Annotation score:
A0A0J9YUR1A0A0J9YUR1_MOUSE
Carbohydrate-responsive element-bin...
Mlxipl
220Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti67D → Y in AAF68175 (PubMed:10780788).Curated1
Sequence conflicti107K → N in AAF68175 (PubMed:10780788).Curated1
Sequence conflicti128R → I in AAF68175 (PubMed:10780788).Curated1
Sequence conflicti138 – 139RK → TR in AAF68175 (PubMed:10780788).Curated2
Sequence conflicti155D → H in AAF68175 (PubMed:10780788).Curated1
Sequence conflicti175E → D in AAF68175 (PubMed:10780788).Curated1
Sequence conflicti183K → V in AAF68175 (PubMed:10780788).Curated1
Sequence conflicti727 – 728QQ → HE in AAF68175 (PubMed:10780788).Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00217458 – 79Missing in isoform 2. CuratedAdd BLAST22
Alternative sequenceiVSP_002175545 – 556AKPEQ…EPPTM → VVLIVLPVPSQA in isoform 5. CuratedAdd BLAST12
Alternative sequenceiVSP_002176557 – 864Missing in isoform 5. CuratedAdd BLAST308
Alternative sequenceiVSP_002177699 – 744VSKAT…INLCQ → GLPTQRPTLVALAGEQSNHA SEDSGVHPDAAAGTGSYAGG GAAAAG in isoform 3. CuratedAdd BLAST46
Alternative sequenceiVSP_002179699 – 714VSKAT…EYILM → LPGLANTEAHIGGARR in isoform 4. CuratedAdd BLAST16
Alternative sequenceiVSP_002180715 – 864Missing in isoform 4. CuratedAdd BLAST150
Alternative sequenceiVSP_002178745 – 864Missing in isoform 3. CuratedAdd BLAST120

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF245475 mRNA Translation: AAK20940.1
AF245476 mRNA Translation: AAK20941.1
AF245477 mRNA Translation: AAK20942.1
AF245478 mRNA Translation: AAK20943.1
AF245479 mRNA Translation: AAK20944.1
AF156604 mRNA Translation: AAF68175.1
CCDSiCCDS39316.1 [Q99MZ3-1]
RefSeqiNP_067430.2, NM_021455.4 [Q99MZ3-1]
UniGeneiMm.34213

Genome annotation databases

EnsembliENSMUST00000005507; ENSMUSP00000005507; ENSMUSG00000005373 [Q99MZ3-1]
ENSMUST00000128691; ENSMUSP00000121348; ENSMUSG00000005373 [Q99MZ3-4]
ENSMUST00000129008; ENSMUSP00000114933; ENSMUSG00000005373 [Q99MZ3-5]
ENSMUST00000153519; ENSMUSP00000122198; ENSMUSG00000005373 [Q99MZ3-3]
GeneIDi58805
KEGGimmu:58805
UCSCiuc008zxt.2 mouse [Q99MZ3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF245475 mRNA Translation: AAK20940.1
AF245476 mRNA Translation: AAK20941.1
AF245477 mRNA Translation: AAK20942.1
AF245478 mRNA Translation: AAK20943.1
AF245479 mRNA Translation: AAK20944.1
AF156604 mRNA Translation: AAF68175.1
CCDSiCCDS39316.1 [Q99MZ3-1]
RefSeqiNP_067430.2, NM_021455.4 [Q99MZ3-1]
UniGeneiMm.34213

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4GNTX-ray2.41B117-137[»]
ProteinModelPortaliQ99MZ3
SMRiQ99MZ3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi208443, 6 interactors
IntActiQ99MZ3, 1 interactor
MINTiQ99MZ3
STRINGi10090.ENSMUSP00000005507

PTM databases

iPTMnetiQ99MZ3
PhosphoSitePlusiQ99MZ3

Proteomic databases

PaxDbiQ99MZ3
PeptideAtlasiQ99MZ3
PRIDEiQ99MZ3

Protocols and materials databases

DNASUi58805
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000005507; ENSMUSP00000005507; ENSMUSG00000005373 [Q99MZ3-1]
ENSMUST00000128691; ENSMUSP00000121348; ENSMUSG00000005373 [Q99MZ3-4]
ENSMUST00000129008; ENSMUSP00000114933; ENSMUSG00000005373 [Q99MZ3-5]
ENSMUST00000153519; ENSMUSP00000122198; ENSMUSG00000005373 [Q99MZ3-3]
GeneIDi58805
KEGGimmu:58805
UCSCiuc008zxt.2 mouse [Q99MZ3-1]

Organism-specific databases

CTDi51085
MGIiMGI:1927999 Mlxipl

Phylogenomic databases

eggNOGiKOG3582 Eukaryota
ENOG410XTA5 LUCA
GeneTreeiENSGT00530000063219
HOGENOMiHOG000113606
HOVERGENiHBG073589
InParanoidiQ99MZ3
KOiK09113
OMAiTAAKPEQ
OrthoDBiEOG091G02A1
PhylomeDBiQ99MZ3
TreeFamiTF324749

Enzyme and pathway databases

ReactomeiR-MMU-163358 PKA-mediated phosphorylation of key metabolic factors
R-MMU-163765 ChREBP activates metabolic gene expression

Miscellaneous databases

PROiPR:Q99MZ3
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000005373 Expressed in 131 organ(s), highest expression level in brown adipose tissue
CleanExiMM_MLXIPL
ExpressionAtlasiQ99MZ3 baseline and differential
GenevisibleiQ99MZ3 MM

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
PfamiView protein in Pfam
PF00010 HLH, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMLXPL_MOUSE
AccessioniPrimary (citable) accession number: Q99MZ3
Secondary accession number(s): Q99MY9
, Q99MZ0, Q99MZ1, Q99MZ2, Q9JLM5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: June 1, 2001
Last modified: September 12, 2018
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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