We will be switching to the new UniProt website soon. Please explore and share your feedback.
Take me to the new website.
UniProtKB - Q99MZ3 (MLXPL_MOUSE)
Protein
Carbohydrate-responsive element-binding protein
Gene
Mlxipl
Organism
Mus musculus (Mouse)
Status
Functioni
Transcriptional repressor. Binds to the canonical and non-canonical E box sequences 5'-CACGTG-3'.
GO - Molecular functioni
- carbohydrate response element binding Source: BHF-UCL
- DNA binding Source: MGI
- DNA-binding transcription factor activity Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: NTNU_SB
- protein heterodimerization activity Source: MGI
- protein kinase binding Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: NTNU_SB
- RNA polymerase II-specific DNA-binding transcription factor binding Source: BHF-UCL
- sequence-specific DNA binding Source: MGI
GO - Biological processi
- cellular response to glucose stimulus Source: MGI
- energy homeostasis Source: UniProtKB
- fatty acid homeostasis Source: UniProtKB
- glucose homeostasis Source: BHF-UCL
- glucose mediated signaling pathway Source: BHF-UCL
- negative regulation of oxidative phosphorylation Source: MGI
- negative regulation of peptidyl-serine phosphorylation Source: MGI
- negative regulation of transcription, DNA-templated Source: BHF-UCL
- negative regulation of transcription by RNA polymerase II Source: MGI
- positive regulation of cell population proliferation Source: MGI
- positive regulation of fatty acid biosynthetic process Source: BHF-UCL
- positive regulation of glycolytic process Source: BHF-UCL
- positive regulation of lipid biosynthetic process Source: MGI
- positive regulation of transcription, DNA-templated Source: BHF-UCL
- positive regulation of transcription by RNA polymerase II Source: BHF-UCL
- regulation of cell cycle Source: MGI
- regulation of glycolytic process Source: MGI
- regulation of transcription by RNA polymerase II Source: MGI
- response to glucose Source: MGI
Keywordsi
Molecular function | DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-MMU-163358, PKA-mediated phosphorylation of key metabolic factors R-MMU-163765, ChREBP activates metabolic gene expression |
Names & Taxonomyi
Protein namesi | Recommended name: Carbohydrate-responsive element-binding proteinShort name: ChREBP Alternative name(s): MLX interactor MLX-interacting protein-like Williams-Beuren syndrome chromosomal region 14 protein homolog |
Gene namesi | Name:Mlxipl Synonyms:Mio, Wbscr14 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1927999, Mlxipl |
VEuPathDBi | HostDB:ENSMUSG00000005373 |
Subcellular locationi
Nucleus
- Nucleus PROSITE-ProRule annotation
Cytosol
- cytosol Source: MGI
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: BHF-UCL
Other locations
- cytoplasm Source: BHF-UCL
- transcription regulator complex Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000127505 | 1 – 864 | Carbohydrate-responsive element-binding proteinAdd BLAST | 864 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 20 | PhosphoserineCombined sources | 1 | |
Modified residuei | 23 | PhosphoserineCombined sources | 1 | |
Modified residuei | 25 | PhosphoserineCombined sources | 1 | |
Modified residuei | 27 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 196 | PhosphoserineBy similarity | 1 | |
Modified residuei | 566 | Phosphoserine; by AMPKBy similarity | 1 | |
Modified residuei | 614 | PhosphoserineBy similarity | 1 | |
Modified residuei | 626 | PhosphoserineCombined sources | 1 | |
Modified residuei | 643 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Phosphorylation at Ser-566 by AMPK inactivates the DNA-binding activity.By similarity
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | Q99MZ3 |
PaxDbi | Q99MZ3 |
PeptideAtlasi | Q99MZ3 |
PRIDEi | Q99MZ3 |
ProteomicsDBi | 290086 [Q99MZ3-1] 290087 [Q99MZ3-2] 290088 [Q99MZ3-3] 290089 [Q99MZ3-4] 290090 [Q99MZ3-5] |
PTM databases
iPTMneti | Q99MZ3 |
PhosphoSitePlusi | Q99MZ3 |
Expressioni
Tissue specificityi
Expressed in the ventricular and intermediate zones of the developing spinal cord of 12.5 dpc embryos. In later embryos expressed in a variety of tissues.
Gene expression databases
Bgeei | ENSMUSG00000005373, Expressed in islet of Langerhans and 173 other tissues |
Genevisiblei | Q99MZ3, MM |
Interactioni
Subunit structurei
Binds DNA as a heterodimer with TCFL4/MLX.
GO - Molecular functioni
- protein heterodimerization activity Source: MGI
- protein kinase binding Source: MGI
- RNA polymerase II-specific DNA-binding transcription factor binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 208443, 6 interactors |
STRINGi | 10090.ENSMUSP00000005507 |
Miscellaneous databases
RNActi | Q99MZ3, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q99MZ3 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 661 – 715 | bHLHPROSITE-ProRule annotationAdd BLAST | 55 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 15 – 41 | DisorderedSequence analysisAdd BLAST | 27 | |
Regioni | 53 – 77 | DisorderedSequence analysisAdd BLAST | 25 | |
Regioni | 332 – 397 | DisorderedSequence analysisAdd BLAST | 66 | |
Regioni | 449 – 468 | DisorderedSequence analysisAdd BLAST | 20 | |
Regioni | 489 – 533 | DisorderedSequence analysisAdd BLAST | 45 | |
Regioni | 547 – 570 | DisorderedSequence analysisAdd BLAST | 24 | |
Regioni | 583 – 602 | DisorderedSequence analysisAdd BLAST | 20 | |
Regioni | 715 – 736 | Leucine-zipperAdd BLAST | 22 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 339 – 373 | Polar residuesSequence analysisAdd BLAST | 35 | |
Compositional biasi | 498 – 533 | Polar residuesSequence analysisAdd BLAST | 36 | |
Compositional biasi | 583 – 601 | Pro residuesSequence analysisAdd BLAST | 19 |
Phylogenomic databases
eggNOGi | KOG3582, Eukaryota |
GeneTreei | ENSGT00940000159210 |
HOGENOMi | CLU_007471_1_0_1 |
InParanoidi | Q99MZ3 |
OMAi | NTAINLC |
OrthoDBi | 388166at2759 |
PhylomeDBi | Q99MZ3 |
TreeFami | TF324749 |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
s (5+)i Sequence
Sequence statusi: Complete.
This entry describes 5 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 5 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q99MZ3-1) [UniParc]FASTAAdd to basket
Also known as: Zeta
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MARALADLSV NLQVPRVVPS PDSDSDTDLE DPSPRRSAGG LHRSQVIHSG
60 70 80 90 100
HFMVSSPHSD SLTRRRDQEG PVGLADFGPR SIDPTLTHLF ECLSLAYSGK
110 120 130 140 150
LVSPKWKNFK GLKLLCRDKI RLNNAIWRAW YIQYVQRRKS PVCGFVTPLQ
160 170 180 190 200
GSEADEHRKP EAVILEGNYW KRRIEVVMRE YHKWRIYYKK RLRKSSREGD
210 220 230 240 250
FLAPKQVEGG WPPPERWCEQ LFSSVVPVLL GGSEEEPGGR QLLDLDCFLS
260 270 280 290 300
DISDTLFTMT QPSPSSLQLP PEDAYVGNAD MIQPDLTPLQ PSLDDFMEIS
310 320 330 340 350
DFFTNYRPPQ TPTSSNYIES PSFGPMADSL FSSGILAPEM PSPASSSSSS
360 370 380 390 400
GMTPHSGNTR LQARNSCSGP LDPNPFLSSE FLLPEDPKTK IPPAPGPTPL
410 420 430 440 450
LPFPTPVKVH GLEPCTPSPF PTMAPPPSLL PEESLLSARF PFTSAPPAPG
460 470 480 490 500
VSTLPAPTTF VPTPQPGPGP VPFSVDHLPH GYLEPVFGPH FTVPQGMQPR
510 520 530 540 550
CKPSSPSPGG QKASPPTLAS ATASPTATAT ARDNNPCLTQ LLRAAKPEQA
560 570 580 590 600
LEPPTMPGTL LRPPESPQDT VSEIPRARAF FPPIPAPTPP RPPPGPATLA
610 620 630 640 650
PPRSLVVPKA ERLSPPASSG SERRLSGDLN SIQPSGALSV HLSPPQTVLS
660 670 680 690 700
RGRVDNNKME NRRITHISAE QKRRFNIKLG FDTLHGLVST LSAQPSLKVS
710 720 730 740 750
KATTLQKTAE YILMLQQERA AMQEEAQQLR DEIEELNAAI NLCQQQLPAT
760 770 780 790 800
GVPITHQRFD QMRDMFDDYV RTRTLHNWKF WVFSILIRPL FESFNGMVST
810 820 830 840 850
ASLHSLRQTS LAWLEQYCSL PALRPTVLNS LRQLSTSTSI LTDPSLVPEQ
860
ATRAVTEGTL GRPL
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF6Q3B6 | F6Q3B6_MOUSE | Carbohydrate-responsive element-bin... | Mlxipl | 181 | Annotation score: | ||
F6Q4B6 | F6Q4B6_MOUSE | Carbohydrate-responsive element-bin... | Mlxipl | 179 | Annotation score: | ||
A0A0J9YUT0 | A0A0J9YUT0_MOUSE | Carbohydrate-responsive element-bin... | Mlxipl | 545 | Annotation score: | ||
A0A0J9YUR1 | A0A0J9YUR1_MOUSE | Carbohydrate-responsive element-bin... | Mlxipl | 220 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 67 | D → Y in AAF68175 (PubMed:10780788).Curated | 1 | |
Sequence conflicti | 107 | K → N in AAF68175 (PubMed:10780788).Curated | 1 | |
Sequence conflicti | 128 | R → I in AAF68175 (PubMed:10780788).Curated | 1 | |
Sequence conflicti | 138 – 139 | RK → TR in AAF68175 (PubMed:10780788).Curated | 2 | |
Sequence conflicti | 155 | D → H in AAF68175 (PubMed:10780788).Curated | 1 | |
Sequence conflicti | 175 | E → D in AAF68175 (PubMed:10780788).Curated | 1 | |
Sequence conflicti | 183 | K → V in AAF68175 (PubMed:10780788).Curated | 1 | |
Sequence conflicti | 727 – 728 | QQ → HE in AAF68175 (PubMed:10780788).Curated | 2 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_002174 | 58 – 79 | Missing in isoform 2. CuratedAdd BLAST | 22 | |
Alternative sequenceiVSP_002175 | 545 – 556 | AKPEQ…EPPTM → VVLIVLPVPSQA in isoform 5. CuratedAdd BLAST | 12 | |
Alternative sequenceiVSP_002176 | 557 – 864 | Missing in isoform 5. CuratedAdd BLAST | 308 | |
Alternative sequenceiVSP_002177 | 699 – 744 | VSKAT…INLCQ → GLPTQRPTLVALAGEQSNHA SEDSGVHPDAAAGTGSYAGG GAAAAG in isoform 3. CuratedAdd BLAST | 46 | |
Alternative sequenceiVSP_002179 | 699 – 714 | VSKAT…EYILM → LPGLANTEAHIGGARR in isoform 4. CuratedAdd BLAST | 16 | |
Alternative sequenceiVSP_002180 | 715 – 864 | Missing in isoform 4. CuratedAdd BLAST | 150 | |
Alternative sequenceiVSP_002178 | 745 – 864 | Missing in isoform 3. CuratedAdd BLAST | 120 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF245475 mRNA Translation: AAK20940.1 AF245476 mRNA Translation: AAK20941.1 AF245477 mRNA Translation: AAK20942.1 AF245478 mRNA Translation: AAK20943.1 AF245479 mRNA Translation: AAK20944.1 AF156604 mRNA Translation: AAF68175.1 |
CCDSi | CCDS39316.1 [Q99MZ3-1] |
RefSeqi | NP_067430.2, NM_021455.4 [Q99MZ3-1] |
Genome annotation databases
Ensembli | ENSMUST00000005507; ENSMUSP00000005507; ENSMUSG00000005373 [Q99MZ3-1] ENSMUST00000128691; ENSMUSP00000121348; ENSMUSG00000005373 [Q99MZ3-4] ENSMUST00000129008; ENSMUSP00000114933; ENSMUSG00000005373 [Q99MZ3-5] ENSMUST00000153519; ENSMUSP00000122198; ENSMUSG00000005373 [Q99MZ3-3] |
GeneIDi | 58805 |
KEGGi | mmu:58805 |
UCSCi | uc008zxt.2, mouse [Q99MZ3-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF245475 mRNA Translation: AAK20940.1 AF245476 mRNA Translation: AAK20941.1 AF245477 mRNA Translation: AAK20942.1 AF245478 mRNA Translation: AAK20943.1 AF245479 mRNA Translation: AAK20944.1 AF156604 mRNA Translation: AAF68175.1 |
CCDSi | CCDS39316.1 [Q99MZ3-1] |
RefSeqi | NP_067430.2, NM_021455.4 [Q99MZ3-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4GNT | X-ray | 2.41 | B | 117-137 | [»] | |
SMRi | Q99MZ3 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 208443, 6 interactors |
STRINGi | 10090.ENSMUSP00000005507 |
PTM databases
iPTMneti | Q99MZ3 |
PhosphoSitePlusi | Q99MZ3 |
Proteomic databases
MaxQBi | Q99MZ3 |
PaxDbi | Q99MZ3 |
PeptideAtlasi | Q99MZ3 |
PRIDEi | Q99MZ3 |
ProteomicsDBi | 290086 [Q99MZ3-1] 290087 [Q99MZ3-2] 290088 [Q99MZ3-3] 290089 [Q99MZ3-4] 290090 [Q99MZ3-5] |
Protocols and materials databases
Antibodypediai | 14346, 353 antibodies from 30 providers |
DNASUi | 58805 |
Genome annotation databases
Ensembli | ENSMUST00000005507; ENSMUSP00000005507; ENSMUSG00000005373 [Q99MZ3-1] ENSMUST00000128691; ENSMUSP00000121348; ENSMUSG00000005373 [Q99MZ3-4] ENSMUST00000129008; ENSMUSP00000114933; ENSMUSG00000005373 [Q99MZ3-5] ENSMUST00000153519; ENSMUSP00000122198; ENSMUSG00000005373 [Q99MZ3-3] |
GeneIDi | 58805 |
KEGGi | mmu:58805 |
UCSCi | uc008zxt.2, mouse [Q99MZ3-1] |
Organism-specific databases
CTDi | 51085 |
MGIi | MGI:1927999, Mlxipl |
VEuPathDBi | HostDB:ENSMUSG00000005373 |
Phylogenomic databases
eggNOGi | KOG3582, Eukaryota |
GeneTreei | ENSGT00940000159210 |
HOGENOMi | CLU_007471_1_0_1 |
InParanoidi | Q99MZ3 |
OMAi | NTAINLC |
OrthoDBi | 388166at2759 |
PhylomeDBi | Q99MZ3 |
TreeFami | TF324749 |
Enzyme and pathway databases
Reactomei | R-MMU-163358, PKA-mediated phosphorylation of key metabolic factors R-MMU-163765, ChREBP activates metabolic gene expression |
Miscellaneous databases
BioGRID-ORCSi | 58805, 4 hits in 64 CRISPR screens |
ChiTaRSi | Mlxipl, mouse |
PROi | PR:Q99MZ3 |
RNActi | Q99MZ3, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000005373, Expressed in islet of Langerhans and 173 other tissues |
Genevisiblei | Q99MZ3, MM |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MLXPL_MOUSE | |
Accessioni | Q99MZ3Primary (citable) accession number: Q99MZ3 Secondary accession number(s): Q99MY9 Q9JLM5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 23, 2002 |
Last sequence update: | June 1, 2001 | |
Last modified: | February 23, 2022 | |
This is version 166 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references