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Protein

Actin-like protein 6B

Gene

Actl6b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Belongs to the neuron-specific chromatin remodeling complex (nBAF complex), as such plays a role in remodeling mononucleosomes in an ATP-dependent fashion, and is required for postmitotic neural development and dendritic outgrowth. During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. ACTL6B/BAF53B is not essential for assembly of the nBAF complex but is required for targeting the complex and CREST to the promoter of genes essential for dendritic growth.3 Publications

GO - Biological processi

  • chromatin assembly or disassembly Source: UniProtKB
  • chromatin remodeling Source: MGI
  • nervous system development Source: UniProtKB
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • spinal cord development Source: Ensembl
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionChromatin regulator
Biological processNeurogenesis, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-3214858 RMTs methylate histone arginines
R-MMU-8939243 RUNX1 interacts with co-factors whose precise effect on RUNX1 targets is not known

Names & Taxonomyi

Protein namesi
Recommended name:
Actin-like protein 6B
Alternative name(s):
53 kDa BRG1-associated factor B
Actin-related protein Baf53b
ArpN-alpha
Short name:
ArpNa
BRG1-associated factor 53B
Short name:
BAF53B
Gene namesi
Name:Actl6b
Synonyms:Actl6, Arpna, Baf53b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1933548 Actl6b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice have lethal defects in neuronal development, including defects in activity-dependent dendritic outgrowth.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000891361 – 426Actin-like protein 6BAdd BLAST426

Proteomic databases

MaxQBiQ99MR0
PaxDbiQ99MR0
PeptideAtlasiQ99MR0
PRIDEiQ99MR0

PTM databases

PhosphoSitePlusiQ99MR0

Expressioni

Tissue specificityi

Restricted to the brain and peripheral nervous tissue (at protein level). Present in virtually all neurons in the cerebral neocortex (layers II-VI), hippocampus (CA1-CA3 region and dentate gyrus), cerebellum (molecular, granular and Purkinje cell layers), spinal cord (dorsally and ventrally), dorsal root ganglion, retina and the olfactory bulb (mitral and granule cell layers). Expressed specifically in postmitotic neurons (at protein level).3 Publications

Developmental stagei

Expression begins near the time of neuronal cell type specification. First apparent at 10.5 dpc in the nervous system, with high levels in the telencephalon and less in the diencephalon, mesencephalon and spinal cord. This pattern of expression persists and becomes more defined at 12.5 dpc and later stages. Detected in dorsal root ganglia, but not in neural crest derivatives that give rise to non-neuronal tissues, including great vessels. Expressed in postmitotic cells in the CNS, but not in actively proliferating precursor cells. At protein level, first detected at 12.5 dpc in nervous tissues. In the developing forebrain, cerebellar primordium and spinal cord, strictly expressed in postmitotic neurons.2 Publications

Inductioni

By retinoic acid of P19 embryonic carcinoma stem cells induced by this treatment to differentiate into neuronal cells.1 Publication

Gene expression databases

BgeeiENSMUSG00000029712 Expressed in 107 organ(s), highest expression level in cerebral cortex
CleanExiMM_ACTL6B
ExpressionAtlasiQ99MR0 baseline and differential
GenevisibleiQ99MR0 MM

Interactioni

Subunit structurei

Component of the multiprotein chromatin-remodeling complexes SWI/SNF: SWI/SNF-A (BAF), SWI/SNF-B (PBAF) and related complexes. The canonical complex contains a catalytic subunit (either SMARCA4/BRG1/BAF190A or SMARCA2/BRM/BAF190B) and at least SMARCE1, ACTL6A/BAF53, SMARCC1/BAF155, SMARCC2/BAF170, and SMARCB1/SNF5/BAF47. Other subunits specific to each of the complexes may also be present permitting several possible combinations developmentally and tissue specific (PubMed:22952240, PubMed:26601204).Component of the BAF complex, which includes at least actin (ACTB), ARID1A/BAF250A, ARID1B/BAF250B, SMARCA2/BRM, SMARCA4/BRG1/BAF190A, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57, SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C (By similarity). Component of neuron-specific chromatin remodeling complex (nBAF complex) composed of at least, ARID1A/BAF250A or ARID1B/BAF250B, SMARCD1/BAF60A or SMARCD2/BAF60B or SMARCD3/BAF60C, SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A, SMARCB1/BAF47, SMARCC1/BAF155, SMARCE1/BAF57, SMARCC2/BAF170, DPF1/BAF45B, DPF3/BAF45C, ACTL6B/BAF53B and actin (ACTB)(PubMed:12368262, PubMed:17640523, PubMed:17920018). Note that the nBAF complex is polymorphic in regard to the ATPase, SMARCA2 and SMARCA4 occupying mutually exclusive positions (PubMed:12368262). May be a component of the SWI/SNF-B (PBAF) chromatin remodeling complex, at least composed of SMARCA4/BRG1, SMARCB1/BAF47/SNF5, ACTL6A/BAF53A or ACTL6B/BAF53B, SMARCE1/BAF57, SMARCD1/BAF60A, SMARCD2/BAF60B, perhaps SMARCD3/BAF60C, SMARCC1/BAF155, SMARCC2/BAF170, PBRM1/BAF180, ARID2/BAF200 and actin (By similarity).2 PublicationsBy similarity3 Publications

Protein-protein interaction databases

BioGridi219970, 3 interactors
ComplexPortaliCPX-1236 SWI/SNF ATP-dependent chromatin remodeling complex, ACTL6B-ARID1A-SMARCA2 variant
CPX-1237 SWI/SNF ATP-dependent chromatin remodeling complex, ACTL6B-ARID1A-SMARCA4 variant
CPX-1238 SWI/SNF ATP-dependent chromatin remodeling complex, ACTL6B-ARID1B-SMARCA2 variant
CPX-1239 SWI/SNF ATP-dependent chromatin remodeling complex, ACTL6B-ARID1B-SMARCA4 variant
CPX-1244 Muscle cell-specific SWI/SNF ATP-dependent chromatin remodeling complex, ACTL6B-ARID1A-SMARCA2 variant
CPX-1245 Muscle cell-specific SWI/SNF ATP-dependent chromatin remodeling complex, ACTL6B-ARID1A-SMARCA4 variant
CPX-1246 Muscle cell-specific SWI/SNF ATP-dependent chromatin remodeling complex, ACTL6B-ARID1B-SMARCA2 variant
CPX-1247 Muscle cell-specific SWI/SNF ATP-dependent chromatin remodeling complex, ACTL6B-ARID1B-SMARCA4 variant
CPX-1250 Polybromo-associated SWI/SNF ATP-dependent chromatin remodeling complex, ACTL6B variant
CPX-1256 Neuron-specific SWI/SNF ATP-dependent chromatin remodeling complex, ARID1A-SMARCA2 variant
CPX-1257 Neuron-specific SWI/SNF ATP-dependent chromatin remodeling complex, ARID1A-SMARCA4 variant
CPX-1258 Neuron-specific SWI/SNF ATP-dependent chromatin remodeling complex, ARID1B-SMARCA2 variant
CPX-1259 Neuron-specific SWI/SNF ATP-dependent chromatin remodeling complex, ARID1B-SMARCA4 variant
CPX-1261 Brain-specific SWI/SNF ATP-dependent chromatin remodeling complex, ARID1A-SMARCA2 variant
CPX-1262 Brain-specific SWI/SNF ATP-dependent chromatin remodeling complex, ARID1A-SMARCA4 variant
CPX-1263 Brain-specific SWI/SNF ATP-dependent chromatin remodeling complex, ARID1B-SMARCA2 variant
CPX-1264 Brain-specific SWI/SNF ATP-dependent chromatin remodeling complex, ARID1B-SMARCA4 variant
IntActiQ99MR0, 3 interactors
MINTiQ99MR0
STRINGi10090.ENSMUSP00000119356

Structurei

3D structure databases

ProteinModelPortaliQ99MR0
SMRiQ99MR0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni39 – 82Essential for mediating its function in dendritic development; may contribute to neuronal-specific targeting1 PublicationAdd BLAST44

Sequence similaritiesi

Belongs to the actin family.Curated

Phylogenomic databases

eggNOGiKOG0679 Eukaryota
COG5277 LUCA
GeneTreeiENSGT00900000140992
HOGENOMiHOG000233340
HOVERGENiHBG003771
InParanoidiQ99MR0
KOiK11652
OMAiSTMERRF
OrthoDBiEOG091G0A1S
PhylomeDBiQ99MR0
TreeFamiTF312863

Family and domain databases

InterProiView protein in InterPro
IPR004000 Actin
IPR004001 Actin_CS
PANTHERiPTHR11937 PTHR11937, 1 hit
PfamiView protein in Pfam
PF00022 Actin, 1 hit
PRINTSiPR00190 ACTIN
SMARTiView protein in SMART
SM00268 ACTIN, 1 hit
PROSITEiView protein in PROSITE
PS00432 ACTINS_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 6 potential isoforms that are computationally mapped.Show allAlign All

Q99MR0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSGGVYGGDE VGALVFDIGS FSVRAGYAGE DCPKADFPTT VGLLAAEEGG
60 70 80 90 100
GLELEGEKEK KGKIFHIDTN ALHVPRDGAE VMSPLKNGMI EDWECFRAIL
110 120 130 140 150
DHTYSKHVKS EPNLHPVLMS EAPWNTRAKR EKLTELMFEQ YNIPAFFLCK
160 170 180 190 200
TAVLTAFANG RSTGLVLDSG ATHTTAIPVH DGYVLQQGIV KSPLAGDFIS
210 220 230 240 250
MQCRELFQEM AIDIIPPYMI AAKEPVREGA PPNWKKKEKL PQVSKSWHNY
260 270 280 290 300
MCNEVIQDFQ ASVLQVSDSP YDEQVAAQMP TVHYEMPNGY NTDYGAERLR
310 320 330 340 350
IPEGLFDPSN VKGLSGNTML GVGHVVTTSI GMCDIDIRPG LYGSVIVTGG
360 370 380 390 400
NTLLQGFTDR LNRELSQKTP PSMRLKLIAS NSTMERKFSP WIGGSILASL
410 420
GTFQQMWISK QEYEEGGKQC VERKCP
Length:426
Mass (Da):46,891
Last modified:June 1, 2001 - v1
Checksum:i7B9CD9B70E1304CE
GO

Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F8WI57F8WI57_MOUSE
Actin-like protein 6B
Actl6b
381Annotation score:
D3Z4L2D3Z4L2_MOUSE
Actin-like protein 6B
Actl6b
116Annotation score:
D3Z5W4D3Z5W4_MOUSE
Actin-like protein 6B
Actl6b
74Annotation score:
D3Z2E2D3Z2E2_MOUSE
Actin-like protein 6B
Actl6b
20Annotation score:
A0A0G2JGU5A0A0G2JGU5_MOUSE
Actin-like protein 6B
Actl6b
65Annotation score:
A0A1C7ZN05A0A1C7ZN05_MOUSE
Actin-like protein 6B
Actl6b
8Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB075226 mRNA Translation: BAB97312.1
AF312033 Genomic DNA Translation: AAK28829.1
CH466529 Genomic DNA Translation: EDL19262.1
BC049539 mRNA Translation: AAH49539.1
CCDSiCCDS19770.1
RefSeqiNP_113581.1, NM_031404.4
UniGeneiMm.31646

Genome annotation databases

EnsembliENSMUST00000139395; ENSMUSP00000119356; ENSMUSG00000029712
GeneIDi83766
KEGGimmu:83766
UCSCiuc009acu.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB075226 mRNA Translation: BAB97312.1
AF312033 Genomic DNA Translation: AAK28829.1
CH466529 Genomic DNA Translation: EDL19262.1
BC049539 mRNA Translation: AAH49539.1
CCDSiCCDS19770.1
RefSeqiNP_113581.1, NM_031404.4
UniGeneiMm.31646

3D structure databases

ProteinModelPortaliQ99MR0
SMRiQ99MR0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi219970, 3 interactors
ComplexPortaliCPX-1236 SWI/SNF ATP-dependent chromatin remodeling complex, ACTL6B-ARID1A-SMARCA2 variant
CPX-1237 SWI/SNF ATP-dependent chromatin remodeling complex, ACTL6B-ARID1A-SMARCA4 variant
CPX-1238 SWI/SNF ATP-dependent chromatin remodeling complex, ACTL6B-ARID1B-SMARCA2 variant
CPX-1239 SWI/SNF ATP-dependent chromatin remodeling complex, ACTL6B-ARID1B-SMARCA4 variant
CPX-1244 Muscle cell-specific SWI/SNF ATP-dependent chromatin remodeling complex, ACTL6B-ARID1A-SMARCA2 variant
CPX-1245 Muscle cell-specific SWI/SNF ATP-dependent chromatin remodeling complex, ACTL6B-ARID1A-SMARCA4 variant
CPX-1246 Muscle cell-specific SWI/SNF ATP-dependent chromatin remodeling complex, ACTL6B-ARID1B-SMARCA2 variant
CPX-1247 Muscle cell-specific SWI/SNF ATP-dependent chromatin remodeling complex, ACTL6B-ARID1B-SMARCA4 variant
CPX-1250 Polybromo-associated SWI/SNF ATP-dependent chromatin remodeling complex, ACTL6B variant
CPX-1256 Neuron-specific SWI/SNF ATP-dependent chromatin remodeling complex, ARID1A-SMARCA2 variant
CPX-1257 Neuron-specific SWI/SNF ATP-dependent chromatin remodeling complex, ARID1A-SMARCA4 variant
CPX-1258 Neuron-specific SWI/SNF ATP-dependent chromatin remodeling complex, ARID1B-SMARCA2 variant
CPX-1259 Neuron-specific SWI/SNF ATP-dependent chromatin remodeling complex, ARID1B-SMARCA4 variant
CPX-1261 Brain-specific SWI/SNF ATP-dependent chromatin remodeling complex, ARID1A-SMARCA2 variant
CPX-1262 Brain-specific SWI/SNF ATP-dependent chromatin remodeling complex, ARID1A-SMARCA4 variant
CPX-1263 Brain-specific SWI/SNF ATP-dependent chromatin remodeling complex, ARID1B-SMARCA2 variant
CPX-1264 Brain-specific SWI/SNF ATP-dependent chromatin remodeling complex, ARID1B-SMARCA4 variant
IntActiQ99MR0, 3 interactors
MINTiQ99MR0
STRINGi10090.ENSMUSP00000119356

PTM databases

PhosphoSitePlusiQ99MR0

Proteomic databases

MaxQBiQ99MR0
PaxDbiQ99MR0
PeptideAtlasiQ99MR0
PRIDEiQ99MR0

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000139395; ENSMUSP00000119356; ENSMUSG00000029712
GeneIDi83766
KEGGimmu:83766
UCSCiuc009acu.1 mouse

Organism-specific databases

CTDi51412
MGIiMGI:1933548 Actl6b

Phylogenomic databases

eggNOGiKOG0679 Eukaryota
COG5277 LUCA
GeneTreeiENSGT00900000140992
HOGENOMiHOG000233340
HOVERGENiHBG003771
InParanoidiQ99MR0
KOiK11652
OMAiSTMERRF
OrthoDBiEOG091G0A1S
PhylomeDBiQ99MR0
TreeFamiTF312863

Enzyme and pathway databases

ReactomeiR-MMU-3214858 RMTs methylate histone arginines
R-MMU-8939243 RUNX1 interacts with co-factors whose precise effect on RUNX1 targets is not known

Miscellaneous databases

ChiTaRSiActl6b mouse
PROiPR:Q99MR0
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029712 Expressed in 107 organ(s), highest expression level in cerebral cortex
CleanExiMM_ACTL6B
ExpressionAtlasiQ99MR0 baseline and differential
GenevisibleiQ99MR0 MM

Family and domain databases

InterProiView protein in InterPro
IPR004000 Actin
IPR004001 Actin_CS
PANTHERiPTHR11937 PTHR11937, 1 hit
PfamiView protein in Pfam
PF00022 Actin, 1 hit
PRINTSiPR00190 ACTIN
SMARTiView protein in SMART
SM00268 ACTIN, 1 hit
PROSITEiView protein in PROSITE
PS00432 ACTINS_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiACL6B_MOUSE
AccessioniPrimary (citable) accession number: Q99MR0
Secondary accession number(s): Q54A88
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: June 1, 2001
Last modified: September 12, 2018
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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