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Protein

Asporin

Gene

Aspn

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds calcium and plays a role in osteoblast-driven collagen biomineralization activity (By similarity). Critical regulator of TGF-beta in articular cartilage and plays an essential role in cartilage homeostasis and osteoarthritis (OA) pathogenesis. Negatively regulates chondrogenesis in the articular cartilage by blocking the TGF-beta/receptor interaction on the cell surface and inhibiting the canonical TGF-beta/Smad signal. Negatively regulates periodontal ligament (PDL) differentiation and mineralization to ensure that the PDL is not ossified and to maintain homeostasis of the tooth-supporting system. Inhibits BMP2-induced cytodifferentiation of PDL cells by preventing its binding to BMPR1B/BMP type-1B receptor, resulting in inhibition of BMP-dependent activation of SMAD proteins. Inhibits the interaction between TGFB1 and TGF-beta receptor type II in the presence of heparin/heparan sulfate in vitro.By similarity4 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processBiomineralization
LigandCalcium

Names & Taxonomyi

Protein namesi
Recommended name:
Asporin
Alternative name(s):
Periodontal ligament-associated protein 1
Short name:
PLAP-1
Gene namesi
Name:Aspn
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1913945 Aspn

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Extracellular matrix, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi170R → E: Partial inhibition of BMP2-induced cytodifferentiation; when associated with E-219. 1 Publication1
Mutagenesisi194E → G or K: Rescues the inhibitory effects of ASPN on BMP2-induced cytodifferentiation. 1 Publication1
Mutagenesisi219R → E: Partial inhibition of BMP2-induced cytodifferentiation; when associated with E-170. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 15Sequence analysisAdd BLAST15
PropeptideiPRO_000003272916 – 32Sequence analysisAdd BLAST17
ChainiPRO_000003273033 – 373AsporinAdd BLAST341

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi48O-linked (GalNAc...) serineBy similarity1
Disulfide bondi68 ↔ 74By similarity
Disulfide bondi72 ↔ 81By similarity
Glycosylationi275N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi326 ↔ 359By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ99MQ4
PaxDbiQ99MQ4
PRIDEiQ99MQ4

PTM databases

PhosphoSitePlusiQ99MQ4

Expressioni

Tissue specificityi

Higher expression in heart, also detected in kidney, stomach, testes, and skin but only weakly in lung, skeletal muscle, small intestine, and thymus. Expressed specifically and predominantly in the periodontal ligament (PDL). During tooth development, strong expression is seen in the dental follicle, which is the progenitor tissue that forms cementum, alveolar bone, and the PDL. Expressed in the perichondria of the maxilla, mandible, vertebrae, and long bones. Predominantly expressed in the perichondrium/periosteum of long bones (at protein level).2 Publications

Developmental stagei

At 12.5 dpc, it is present in the mandibular as well as maxillary components of the first branchial arch. Also detected in the thoracic body wall adjacent to the heart. At 13.5 dpc, it is detected in the mesenchyme lateral to Meckel's cartilage. Pronounced expression is observed in the perichondrium of the humerus, ribs, and scapula. At 14.5 dpc, it is detected in the mesenchymal condensations lateral to Meckel's cartilage, in the perichondrium surrounding the central cartilaginous elements of the vertebra and also in the dermal mesenchyme. At 15.5 dpc, it is expressed in the perichondrium/periosteum of the long bones (i.e. femur, tibia, and fibula), some of the flat bones at the base of the skull (i.e. sphenoid bone), ribs, clavicle, and vertebrae. Also detected in the intramembranous bones of the maxilla and mandible (alveolar bone) and a strong expression is observed in sagittal sections of the subcutaneous muscles or panniculus carnosus of the thorax, trunk, and head/ neck (platysma muscle) region. Very little expression is detected in the major parenchymal organs (with the exception of the large bronchi of the lung). Its expression is prominent in the developing mouse skeleton, particularly in the perichondrium/periosteum of cartilage/bone, and is also found in other specialized connective tissues such as tendon, sclera, the connective tissue sheath surrounding muscle and dermis. In the sclera of the eye it is first detected at 15.5 dpc and stronger expression was detected at 17.5 dpc.1 Publication

Inductioni

By TGFB1. Induction requires ALK5 kinase activity and SMAD3.1 Publication

Gene expression databases

BgeeiENSMUSG00000021388 Expressed in 230 organ(s), highest expression level in diaphysis of femur
CleanExiMM_ASPN
ExpressionAtlasiQ99MQ4 baseline and differential
GenevisibleiQ99MQ4 MM

Interactioni

Subunit structurei

Interacts with type I collagen. DCN can inhibit collagen binding (By similarity). Interacts with TGFB1, TGFB2 and TGFB3. DCN, BGN, and FMOD inhibit binding to TGFB1. Interacts with BMP2. Interacts in vitro with type II collagen.By similarity3 Publications

Protein-protein interaction databases

IntActiQ99MQ4, 1 interactor
MINTiQ99MQ4
STRINGi10090.ENSMUSP00000021820

Structurei

3D structure databases

ProteinModelPortaliQ99MQ4
SMRiQ99MQ4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini59 – 95LRRNTAdd BLAST37
Repeati96 – 117LRR 1Add BLAST22
Repeati120 – 141LRR 2Add BLAST22
Repeati144 – 166LRR 3Add BLAST23
Repeati167 – 186LRR 4Add BLAST20
Repeati189 – 212LRR 5Add BLAST24
Repeati235 – 255LRR 6Add BLAST21
Repeati259 – 280LRR 7Add BLAST22
Repeati283 – 305LRR 8Add BLAST23
Repeati306 – 327LRR 9Add BLAST22
Repeati328 – 349LRR 10Add BLAST22
Repeati350 – 373LRR 11Add BLAST24

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni159 – 205Interaction with TGFB1Add BLAST47

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi36 – 46Poly-AspAdd BLAST11
Compositional biasi68 – 81Cys-richAdd BLAST14

Domaini

The repeats LRR 9, LRR 10 and LRR 11 are involved in binding type I collagen. The poly-Asp region is involved in binding calcium (By similarity). The LRR 5 repeat can inhibit BMP2-induced cytodifferentiation and may be involved in the interaction with BMP2.By similarity1 Publication

Sequence similaritiesi

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal

Phylogenomic databases

eggNOGiKOG0619 Eukaryota
COG4886 LUCA
GeneTreeiENSGT00760000118969
HOGENOMiHOG000261690
HOVERGENiHBG016052
InParanoidiQ99MQ4
KOiK08120
OMAiNDFCPTV
OrthoDBiEOG091G044B
PhylomeDBiQ99MQ4
TreeFamiTF334562

Family and domain databases

Gene3Di3.80.10.10, 1 hit
InterProiView protein in InterPro
IPR028548 Asporin
IPR001611 Leu-rich_rpt
IPR003591 Leu-rich_rpt_typical-subtyp
IPR032675 LRR_dom_sf
IPR000372 LRRNT
IPR016352 SLRP_I_decor/aspor/byglycan
PANTHERiPTHR44053:SF3 PTHR44053:SF3, 1 hit
PfamiView protein in Pfam
PF13855 LRR_8, 3 hits
PF01462 LRRNT, 1 hit
PIRSFiPIRSF002490 SLRP_I, 1 hit
SMARTiView protein in SMART
SM00369 LRR_TYP, 7 hits
SM00013 LRRNT, 1 hit
PROSITEiView protein in PROSITE
PS51450 LRR, 7 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99MQ4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKEYVMLLLL AVCSAKPFFS PSHTALKNMM LKDMEDTDDD DNDDDDNSLF
60 70 80 90 100
PTKEPVNPFF PFDLFPTCPF GCQCYSRVVH CSDLGLTSVP NNIPFDTRMV
110 120 130 140 150
DLQNNKIKEI KENDFKGLTS LYALILNNNK LTKIHPKTFL TTKKLRRLYL
160 170 180 190 200
SHNQLSEIPL NLPKSLAELR IHDNKVKKIQ KDTFKGMNAL HVLEMSANPL
210 220 230 240 250
ENNGIEPGAF EGVTVFHIRI AEAKLTSIPK GLPPTLLELH LDFNKISTVE
260 270 280 290 300
LEDLKRYREL QRLGLGNNRI TDIENGTFAN IPRVREIHLE HNKLKKIPSG
310 320 330 340 350
LQELKYLQII FLHYNSIAKV GVNDFCPTVP KMKKSLYSAI SLFNNPMKYW
360 370
EIQPATFRCV LGRMSVQLGN VGK
Length:373
Mass (Da):42,573
Last modified:June 1, 2001 - v1
Checksum:iA50C4C82AABCFC35
GO

Sequence cautioni

The sequence AK014504 differs from that shown. Reason: Erroneous termination at position 238. Translated as Glu.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF316825 mRNA Translation: AAK35162.1
AK014504 mRNA No translation available.
BC034888 mRNA Translation: AAH34888.1
CCDSiCCDS26503.1
RefSeqiNP_001165952.1, NM_001172481.1
NP_079987.2, NM_025711.3
UniGeneiMm.383216

Genome annotation databases

EnsembliENSMUST00000021820; ENSMUSP00000021820; ENSMUSG00000021388
ENSMUST00000177948; ENSMUSP00000136728; ENSMUSG00000021388
GeneIDi66695
KEGGimmu:66695
UCSCiuc007qjm.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF316825 mRNA Translation: AAK35162.1
AK014504 mRNA No translation available.
BC034888 mRNA Translation: AAH34888.1
CCDSiCCDS26503.1
RefSeqiNP_001165952.1, NM_001172481.1
NP_079987.2, NM_025711.3
UniGeneiMm.383216

3D structure databases

ProteinModelPortaliQ99MQ4
SMRiQ99MQ4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ99MQ4, 1 interactor
MINTiQ99MQ4
STRINGi10090.ENSMUSP00000021820

PTM databases

PhosphoSitePlusiQ99MQ4

Proteomic databases

MaxQBiQ99MQ4
PaxDbiQ99MQ4
PRIDEiQ99MQ4

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021820; ENSMUSP00000021820; ENSMUSG00000021388
ENSMUST00000177948; ENSMUSP00000136728; ENSMUSG00000021388
GeneIDi66695
KEGGimmu:66695
UCSCiuc007qjm.2 mouse

Organism-specific databases

CTDi54829
MGIiMGI:1913945 Aspn

Phylogenomic databases

eggNOGiKOG0619 Eukaryota
COG4886 LUCA
GeneTreeiENSGT00760000118969
HOGENOMiHOG000261690
HOVERGENiHBG016052
InParanoidiQ99MQ4
KOiK08120
OMAiNDFCPTV
OrthoDBiEOG091G044B
PhylomeDBiQ99MQ4
TreeFamiTF334562

Miscellaneous databases

PROiPR:Q99MQ4
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021388 Expressed in 230 organ(s), highest expression level in diaphysis of femur
CleanExiMM_ASPN
ExpressionAtlasiQ99MQ4 baseline and differential
GenevisibleiQ99MQ4 MM

Family and domain databases

Gene3Di3.80.10.10, 1 hit
InterProiView protein in InterPro
IPR028548 Asporin
IPR001611 Leu-rich_rpt
IPR003591 Leu-rich_rpt_typical-subtyp
IPR032675 LRR_dom_sf
IPR000372 LRRNT
IPR016352 SLRP_I_decor/aspor/byglycan
PANTHERiPTHR44053:SF3 PTHR44053:SF3, 1 hit
PfamiView protein in Pfam
PF13855 LRR_8, 3 hits
PF01462 LRRNT, 1 hit
PIRSFiPIRSF002490 SLRP_I, 1 hit
SMARTiView protein in SMART
SM00369 LRR_TYP, 7 hits
SM00013 LRRNT, 1 hit
PROSITEiView protein in PROSITE
PS51450 LRR, 7 hits
ProtoNetiSearch...

Entry informationi

Entry nameiASPN_MOUSE
AccessioniPrimary (citable) accession number: Q99MQ4
Secondary accession number(s): Q9D6A2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2002
Last sequence update: June 1, 2001
Last modified: November 7, 2018
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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