Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Serine/threonine-protein kinase PINK1, mitochondrial

Gene

Pink1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Protects against mitochondrial dysfunction during cellular stress by phosphorylating mitochondrial proteins (PubMed:24652937). Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy) by mediating activation and translocation of PRKN (PubMed:24784582). Targets PRKN to dysfunctional depolarized mitochondria through the phosphorylation of MFN2 (By similarity). Activates PRKN in 2 steps: (1) by mediating phosphorylation at 'Ser-65' of PRKN and (2) mediating phosphorylation of ubiquitin, converting PRKN to its fully-active form (PubMed:24784582). Required for ubiquinone reduction by mitochondrial complex I by mediating phosphorylation of complex I subunit NDUFA10 (PubMed:24652937).By similarity2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei186ATPPROSITE-ProRule annotation1
Active sitei361Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi162 – 170ATPPROSITE-ProRule annotationBy similarity9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processAutophagy
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-5205685 Pink/Parkin Mediated Mitophagy

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase PINK1, mitochondrial (EC:2.7.11.11 Publication)
Alternative name(s):
BRPK
PTEN-induced putative kinase protein 1
Gene namesi
Name:Pink1
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1916193 Pink1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini78 – 93Mitochondrial intermembraneSequence analysisAdd BLAST16
Transmembranei94 – 110HelicalSequence analysisAdd BLAST17
Topological domaini111 – 580CytoplasmicSequence analysisAdd BLAST470

Keywords - Cellular componenti

Cytoplasm, Membrane, Mitochondrion, Mitochondrion inner membrane, Mitochondrion outer membrane

Pathology & Biotechi

Disruption phenotypei

Mitochondrial dysfunctions (PubMed:18687901, PubMed:20049710). Mice do not show gross structural defects in mitochondria, although the number of larger mitochondria is selectively increased (PubMed:18687901). Mitochondrial respiration is impaired in the striatum, which is rich in dopaminergic terminals, but not in the cerebral cortex in young mice (PubMed:18687901). Mitochondrial respiration activities in the cerebral cortex are decreased in 2 years old mice (PubMed:18687901). Mice show defects in mitochondrial complex I and a decrease in mitochondrial membrane potential (PubMed:20049710).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 77MitochondrionSequence analysisAdd BLAST77
ChainiPRO_000002437078 – 580Serine/threonine-protein kinase PINK1, mitochondrialAdd BLAST503

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei227PhosphoserineBy similarity1
Modified residuei401PhosphoserineBy similarity1

Post-translational modificationi

Autophosphorylated.By similarity
Two shorter forms of 55 kDa and 48 kDa seem to be produced by proteolytic cleavage and localize mainly in cytosol.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ99MQ3
PRIDEiQ99MQ3

PTM databases

iPTMnetiQ99MQ3
PhosphoSitePlusiQ99MQ3

Expressioni

Tissue specificityi

High levels expressed in testis, lower levels in brain, heart, lung, liver and kidney.1 Publication

Gene expression databases

BgeeiENSMUSG00000028756 Expressed in 282 organ(s), highest expression level in gastrocnemius
CleanExiMM_PINK1
ExpressionAtlasiQ99MQ3 baseline and differential
GenevisibleiQ99MQ3 MM

Interactioni

Subunit structurei

Interacts with PRKN and FBXO7 (By similarity). Forms a complex with PRKN and PARK7 (PubMed:19229105).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi213129, 2 interactors
IntActiQ99MQ3, 3 interactors
STRINGi10090.ENSMUSP00000030536

Structurei

3D structure databases

ProteinModelPortaliQ99MQ3
SMRiQ99MQ3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini156 – 510Protein kinasePROSITE-ProRule annotationCuratedAdd BLAST355

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4158 Eukaryota
ENOG410YE6P LUCA
GeneTreeiENSGT00390000001206
HOVERGENiHBG053601
InParanoidiQ99MQ3
KOiK05688
OMAiFLVMKNY
OrthoDBiEOG091G03V6
PhylomeDBiQ99MQ3
TreeFamiTF313183

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 3 hits
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.iShow all

Q99MQ3-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAVRQALGRG LQLGRALLLR FAPKPGPLFG WGKPGPAAAW GRGERPGQVV
60 70 80 90 100
SPGAQPRPVG LPLPDRYRFF RQSVAGLAAR IQRQFMVRAR GGAGPCGRAV
110 120 130 140 150
FLAFGLGLGL IEEKQAEGRR AASACQEIQA IFTQKTKRVS DPLDTRCWQG
160 170 180 190 200
FRLEDYLIGQ AIGKGCNAAV YEATMPTLPQ HLEKAKHLGL IGKGPDVVLK
210 220 230 240 250
GADGEQAPGT PTFPFAIKMM WNISAGSSSE AILSKMSQEL VPASRVALAG
260 270 280 290 300
EYGAVTYRRS RDGPKQLAPH PNIIRVFRAF TSSVPLLPGA LADYPDMLPP
310 320 330 340 350
HYYPEGLGHG RTLFLVMKNY PCTLRQYLEE QTPSSRLATM MTLQLLEGVD
360 370 380 390 400
HLVQQGIAHR DLKSDNILVE WDSDGCPWLV ISDFGCCLAD QHVGLRLPFN
410 420 430 440 450
SSSVERGGNG SLMAPEVSTA HSGPSAVIDY SKADTWAVGA IAYEIFGLAN
460 470 480 490 500
PFYGQGSAHL ESRSYQEAQL PEMPESVPPE ARRLVRSLLQ REASKRPSAR
510 520 530 540 550
LAANVLHLSL WGEHLLALKN LKLDKMIAWL LQQSAATLLA DRLREKSCVE
560 570 580
TKLQMLFLAN LECEALCQAA LLLSSWRAAP
Length:580
Mass (Da):63,181
Last modified:June 7, 2004 - v2
Checksum:i8AB6994B8B4E443E
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A2AM78A2AM78_MOUSE
Serine/threonine-protein kinase PIN...
Pink1
550Annotation score:
A2AM76A2AM76_MOUSE
Serine/threonine-protein kinase PIN...
Pink1
168Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti9R → P in BAB55651 (PubMed:11494141).Curated1
Sequence conflicti48Q → R in AAK28061 (PubMed:14607334).Curated1
Sequence conflicti249A → D in AAK28061 (PubMed:14607334).Curated1
Sequence conflicti425S → G in AAH54349 (PubMed:15489334).Curated1
Sequence conflicti475E → K in AAK28061 (PubMed:14607334).Curated1
Sequence conflicti476S → L in AAH54349 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB053476 mRNA Translation: BAB55651.1
AF316872 mRNA Translation: AAK28061.1
AL807249 Genomic DNA No translation available.
BC044743 mRNA Translation: AAH44743.1
BC054349 mRNA Translation: AAH54349.1
BC067066 mRNA Translation: AAH67066.1
CCDSiCCDS18825.1
RefSeqiNP_081156.2, NM_026880.2
UniGeneiMm.18539

Genome annotation databases

EnsembliENSMUST00000030536; ENSMUSP00000030536; ENSMUSG00000028756
GeneIDi68943
KEGGimmu:68943
UCSCiuc008vku.1 mouse

Similar proteinsi

Entry informationi

Entry nameiPINK1_MOUSE
AccessioniPrimary (citable) accession number: Q99MQ3
Secondary accession number(s): A2AM77
, Q7TMZ3, Q811I8, Q91XU5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: September 12, 2018
This is version 145 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again