UniProtKB - Q99MN9 (PCCB_MOUSE)
Protein
Propionyl-CoA carboxylase beta chain, mitochondrial
Gene
Pccb
Organism
Mus musculus (Mouse)
Status
Functioni
This is one of the 2 subunits of the biotin-dependent propionyl-CoA carboxylase (PCC), a mitochondrial enzyme involved in the catabolism of odd chain fatty acids, branched-chain amino acids isoleucine, threonine, methionine, and valine and other metabolites. Propionyl-CoA carboxylase catalyzes the carboxylation of propionyl-CoA/propanoyl-CoA to D-methylmalonyl-CoA/(S)-methylmalonyl-CoA (By similarity). Within the holoenzyme, the alpha subunit catalyzes the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain, while the beta subunit then transfers the carboxyl group from carboxylated biotin to propionyl-CoA (By similarity). Propionyl-CoA carboxylase also significantly acts on butyryl-CoA/butanoyl-CoA, which is converted to ethylmalonyl-CoA/(2S)-ethylmalonyl-CoA (By similarity). Other alternative minor substrates include (2E)-butenoyl-CoA/crotonoyl-CoA (By similarity).By similarity
Catalytic activityi
- EC:6.4.1.3By similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
: propanoyl-CoA degradation Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes succinyl-CoA from propanoyl-CoA.By similarityProteins known to be involved in the 3 steps of the subpathway in this organism are:
- Propionyl-CoA carboxylase alpha chain, mitochondrial (Pcca), Propionyl-CoA carboxylase beta chain, mitochondrial (Pccb), Propanoyl-CoA:carbon dioxide ligase subunit alpha (Pcca)
- no protein annotated in this organism
- no protein annotated in this organism
View all proteins of this organism that are known to be involved in the subpathway that synthesizes succinyl-CoA from propanoyl-CoA, the pathway propanoyl-CoA degradation and in Metabolic intermediate metabolism.
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- propionyl-CoA carboxylase activity Source: UniProtKB
Keywordsi
Molecular function | Ligase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 6.4.1.3, 3474 |
Reactomei | R-MMU-196780, Biotin transport and metabolism R-MMU-71032, Propionyl-CoA catabolism |
UniPathwayi | UPA00945;UER00908 |
Names & Taxonomyi
Protein namesi | Recommended name: Propionyl-CoA carboxylase beta chain, mitochondrial1 Publication (EC:6.4.1.3By similarity)Short name: PCCase subunit beta Alternative name(s): Propanoyl-CoA:carbon dioxide ligase subunit beta |
Gene namesi | Name:PccbImported |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1914154, Pccb |
Subcellular locationi
Mitochondrion
- Mitochondrion matrix By similarity
Mitochondrion
- mitochondrial matrix Source: UniProtKB
- mitochondrion Source: MGI
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 28 | MitochondrionBy similarityAdd BLAST | 28 | |
ChainiPRO_0000000294 | 29 – 541 | Propionyl-CoA carboxylase beta chain, mitochondrialAdd BLAST | 513 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 73 | PhosphoserineBy similarity | 1 | |
Modified residuei | 101 | N6-acetyllysine; alternateCombined sources | 1 | |
Modified residuei | 101 | N6-succinyllysine; alternateCombined sources | 1 | |
Modified residuei | 250 | N6-succinyllysineCombined sources | 1 | |
Modified residuei | 476 | N6-acetyllysine; alternateCombined sources | 1 | |
Modified residuei | 476 | N6-succinyllysine; alternateCombined sources | 1 | |
Modified residuei | 491 | N6-acetyllysine; alternateCombined sources | 1 | |
Modified residuei | 491 | N6-succinyllysine; alternateCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q99MN9 |
jPOSTi | Q99MN9 |
MaxQBi | Q99MN9 |
PaxDbi | Q99MN9 |
PeptideAtlasi | Q99MN9 |
PRIDEi | Q99MN9 |
2D gel databases
REPRODUCTION-2DPAGEi | Q99MN9 |
PTM databases
iPTMneti | Q99MN9 |
PhosphoSitePlusi | Q99MN9 |
SwissPalmi | Q99MN9 |
Expressioni
Tissue specificityi
Broadly expressed. Most abundantly expressed in the kidney, liver, small intestine and stomach.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000032527, Expressed in adult mammalian kidney and 296 other tissues |
ExpressionAtlasi | Q99MN9, baseline and differential |
Genevisiblei | Q99MN9, MM |
Interactioni
Subunit structurei
The holoenzyme is a dodecamer composed of 6 PCCA/alpha subunits and 6 PCCB/beta subunits.
By similarityProtein-protein interaction databases
BioGRIDi | 211800, 6 interactors |
IntActi | Q99MN9, 4 interactors |
MINTi | Q99MN9 |
STRINGi | 10090.ENSMUSP00000035116 |
Miscellaneous databases
RNActi | Q99MN9, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 34 – 292 | CoA carboxyltransferase N-terminalPROSITE-ProRule annotationAdd BLAST | 259 | |
Domaini | 296 – 535 | CoA carboxyltransferase C-terminalPROSITE-ProRule annotationAdd BLAST | 240 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 34 – 535 | CarboxyltransferasePROSITE-ProRule annotationAdd BLAST | 502 | |
Regioni | 327 – 360 | Acyl-CoA bindingSequence analysisAdd BLAST | 34 |
Domaini
The beta subunit contains the carboxyl transferase (CT) domain.By similarity
Sequence similaritiesi
Belongs to the AccD/PCCB family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG0540, Eukaryota |
GeneTreei | ENSGT00940000157741 |
HOGENOMi | CLU_018822_6_0_1 |
InParanoidi | Q99MN9 |
OMAi | CHFSVMA |
OrthoDBi | 402617at2759 |
TreeFami | TF314350 |
Family and domain databases
InterProi | View protein in InterPro IPR034733, AcCoA_carboxyl IPR029045, ClpP/crotonase-like_dom_sf IPR011763, COA_CT_C IPR011762, COA_CT_N |
Pfami | View protein in Pfam PF01039, Carboxyl_trans, 1 hit |
SUPFAMi | SSF52096, SSF52096, 2 hits |
PROSITEi | View protein in PROSITE PS50989, COA_CT_CTER, 1 hit PS50980, COA_CT_NTER, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
Q99MN9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAAAIRIRAV AAGARLSVLN CGLGITTRGL CSQPVSVKER IDNKRHAALL
60 70 80 90 100
GGGQRRIDAQ HKRGKLTARE RISLLLDPGS FMESDMFVEH RCADFGMAAD
110 120 130 140 150
KNKFPGDSVV TGRGRINGRL VYVFSQDFTV FGGSLSGAHA QKICKIMDQA
160 170 180 190 200
ITVGAPVIGL NDSGGARIQE GVESLAGYAD IFLRNVTASG VIPQISLIMG
210 220 230 240 250
PCAGGAVYSP ALTDFTFMVK DTSYLFITGP EVVKSVTNED VTQEQLGGAK
260 270 280 290 300
THTTVSGVAH RAFDNDVDAL CNLREFFNFL PLSSQDPAPI RECHDPSDRL
310 320 330 340 350
VPELDTVVPL ESSKAYNMLD IIHAVIDERE FFEIMPSYAK NIVVGFARMN
360 370 380 390 400
GRTVGIVGNQ PNVASGCLDI NSSVKGARFV RFCDAFNIPL ITFVDVPGFL
410 420 430 440 450
PGTAQEYGGI IRHGAKLLYA FAEATVPKIT VITRKAYGGA YDVMSSKHLL
460 470 480 490 500
GDTNYAWPTA EIAVMGAKGA VEIIFKGHQD VEAAQAEYVE KFANPFPAAV
510 520 530 540
RGFVDDIIQP SSTRARICCD LEVLASKKVH RPWRKHANIP L
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD3YZC1 | D3YZC1_MOUSE | Propionyl-CoA carboxylase beta chai... | Pccb | 256 | Annotation score: | ||
E9Q1J7 | E9Q1J7_MOUSE | Propionyl-CoA carboxylase beta chai... | Pccb | 504 | Annotation score: | ||
A0A087WQV1 | A0A087WQV1_MOUSE | Propionyl-CoA carboxylase beta chai... | Pccb | 132 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 340 | K → L in AAK28525 (PubMed:11245989).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF327060 mRNA Translation: AAK28525.1 AK004976 mRNA Translation: BAB23712.1 AK050366 mRNA Translation: BAC34211.1 |
CCDSi | CCDS23442.1 |
RefSeqi | NP_001298078.1, NM_001311149.1 NP_080111.1, NM_025835.3 |
Genome annotation databases
Ensembli | ENSMUST00000035116; ENSMUSP00000035116; ENSMUSG00000032527 |
GeneIDi | 66904 |
KEGGi | mmu:66904 |
UCSCi | uc009rfc.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF327060 mRNA Translation: AAK28525.1 AK004976 mRNA Translation: BAB23712.1 AK050366 mRNA Translation: BAC34211.1 |
CCDSi | CCDS23442.1 |
RefSeqi | NP_001298078.1, NM_001311149.1 NP_080111.1, NM_025835.3 |
3D structure databases
SMRi | Q99MN9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 211800, 6 interactors |
IntActi | Q99MN9, 4 interactors |
MINTi | Q99MN9 |
STRINGi | 10090.ENSMUSP00000035116 |
PTM databases
iPTMneti | Q99MN9 |
PhosphoSitePlusi | Q99MN9 |
SwissPalmi | Q99MN9 |
2D gel databases
REPRODUCTION-2DPAGEi | Q99MN9 |
Proteomic databases
EPDi | Q99MN9 |
jPOSTi | Q99MN9 |
MaxQBi | Q99MN9 |
PaxDbi | Q99MN9 |
PeptideAtlasi | Q99MN9 |
PRIDEi | Q99MN9 |
Protocols and materials databases
Antibodypediai | 33408, 198 antibodies |
DNASUi | 66904 |
Genome annotation databases
Ensembli | ENSMUST00000035116; ENSMUSP00000035116; ENSMUSG00000032527 |
GeneIDi | 66904 |
KEGGi | mmu:66904 |
UCSCi | uc009rfc.1, mouse |
Organism-specific databases
CTDi | 5096 |
MGIi | MGI:1914154, Pccb |
Phylogenomic databases
eggNOGi | KOG0540, Eukaryota |
GeneTreei | ENSGT00940000157741 |
HOGENOMi | CLU_018822_6_0_1 |
InParanoidi | Q99MN9 |
OMAi | CHFSVMA |
OrthoDBi | 402617at2759 |
TreeFami | TF314350 |
Enzyme and pathway databases
UniPathwayi | UPA00945;UER00908 |
BRENDAi | 6.4.1.3, 3474 |
Reactomei | R-MMU-196780, Biotin transport and metabolism R-MMU-71032, Propionyl-CoA catabolism |
Miscellaneous databases
BioGRID-ORCSi | 66904, 0 hits in 17 CRISPR screens |
ChiTaRSi | Pccb, mouse |
PROi | PR:Q99MN9 |
RNActi | Q99MN9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000032527, Expressed in adult mammalian kidney and 296 other tissues |
ExpressionAtlasi | Q99MN9, baseline and differential |
Genevisiblei | Q99MN9, MM |
Family and domain databases
InterProi | View protein in InterPro IPR034733, AcCoA_carboxyl IPR029045, ClpP/crotonase-like_dom_sf IPR011763, COA_CT_C IPR011762, COA_CT_N |
Pfami | View protein in Pfam PF01039, Carboxyl_trans, 1 hit |
SUPFAMi | SSF52096, SSF52096, 2 hits |
PROSITEi | View protein in PROSITE PS50989, COA_CT_CTER, 1 hit PS50980, COA_CT_NTER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PCCB_MOUSE | |
Accessioni | Q99MN9Primary (citable) accession number: Q99MN9 Secondary accession number(s): Q9DBG2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 24, 2005 |
Last sequence update: | July 27, 2011 | |
Last modified: | December 2, 2020 | |
This is version 134 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families