UniProtKB - Q99MK9 (RASF1_MOUSE)
Ras association domain-containing protein 1
Rassf1
Functioni
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 51 – 101 | Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST | 51 |
GO - Molecular functioni
- identical protein binding Source: MGI
- metal ion binding Source: UniProtKB-KW
- protein N-terminus binding Source: MGI
GO - Biological processi
- cell cycle arrest Source: UniProtKB
- cellular response to DNA damage stimulus Source: UniProtKB
- negative regulation of cell cycle arrest Source: UniProtKB
- positive regulation of protein ubiquitination Source: UniProtKB
- protein stabilization Source: UniProtKB
- Ras protein signal transduction Source: UniProtKB
- regulation of microtubule cytoskeleton organization Source: InterPro
- signal transduction Source: GO_Central
Keywordsi
Biological process | Cell cycle |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Ras association domain-containing protein 1Alternative name(s): Protein 123F2 |
Gene namesi | Name:Rassf1Imported |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1928386, Rassf1 |
Subcellular locationi
Cytoskeleton
- cytoskeleton By similarity
- centrosome By similarity
- spindle By similarity
- spindle pole By similarity
Note: Localizes to cytoplasmic microtubules during interphase, to bipolar centrosomes associated with microtubules during prophase, to spindle fibers and spindle poles at metaphase and anaphase, to the midzone during early telophase, and to the midbody in late telophase in cells. Colocalizes with MDM2 in the nucleus (By similarity).By similarity
Nucleus
- Nucleus By similarity
Note: Predominantly nuclear.By similarity
Cytoskeleton
- microtubule Source: UniProtKB-KW
- microtubule cytoskeleton Source: UniProtKB
- microtubule organizing center Source: UniProtKB-SubCell
- spindle pole Source: UniProtKB-SubCell
Nucleus
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB-KW
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, Microtubule, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000068892 | 2 – 340 | Ras association domain-containing protein 1Add BLAST | 339 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineBy similarity | 1 | |
Modified residuei | 2 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q99MK9 |
PaxDbi | Q99MK9 |
PRIDEi | Q99MK9 |
ProteomicsDBi | 255104 [Q99MK9-1] 255105 [Q99MK9-2] |
PTM databases
iPTMneti | Q99MK9 |
PhosphoSitePlusi | Q99MK9 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000010067, Expressed in optic fissure and 298 other tissues |
ExpressionAtlasi | Q99MK9, baseline and differential |
Genevisiblei | Q99MK9, MM |
Interactioni
Subunit structurei
Interacts with MAP1S and XPA (By similarity). Binds to the N-terminal of CDC20 during prometaphase (By similarity). Binds to STK3/MST2 and STK4/MST1 (By similarity). Isoform A interacts with MOAP1 and E4F1 (By similarity). Recruited to the TNFRSF1A and TNFRSF10A complexes in response to their respective cognate ligand, after internalization (By similarity). Can self-associate (By similarity). Isoform A interacts with RSSF5 and probably associates with HRAS via a RSSF1 isoform A-RSSF5 heterodimer (By similarity).
Part of a complex with MDM2, DAXX, RASSF1 and USP7 (By similarity). Isoform A interacts (via C-terminus) with DAXX (via N-terminus); the interaction is independent of MDM2 and TP53 (By similarity). Isoform A interacts (via N-terminus) with MDM2 (via C-terminus); the interaction is independent of TP53 (By similarity). Isoform C interacts with ECM2 (PubMed:17335777).
By similarity1 PublicationGO - Molecular functioni
- identical protein binding Source: MGI
- protein N-terminus binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 207877, 32 interactors |
IntActi | Q99MK9, 31 interactors |
MINTi | Q99MK9 |
STRINGi | 10090.ENSMUSP00000010211 |
Miscellaneous databases
RNActi | Q99MK9, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 194 – 288 | Ras-associatingPROSITE-ProRule annotationAdd BLAST | 95 | |
Domaini | 290 – 337 | SARAHPROSITE-ProRule annotationAdd BLAST | 48 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 2 – 115 | Mediates interaction with E4F1By similarityAdd BLAST | 114 | |
Regioni | 311 – 314 | MOAP1-bindingBy similarity | 4 |
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 51 – 101 | Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST | 51 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG4239, Eukaryota |
GeneTreei | ENSGT00940000155664 |
HOGENOMi | CLU_045544_0_1_1 |
InParanoidi | Q99MK9 |
OMAi | GTPSFEM |
PhylomeDBi | Q99MK9 |
TreeFami | TF319243 |
Family and domain databases
CDDi | cd00029, C1, 1 hit |
InterProi | View protein in InterPro IPR033614, C-RASSF IPR002219, PE/DAG-bd IPR000159, RA_dom IPR033600, RASSF1 IPR011524, SARAH_dom IPR029071, Ubiquitin-like_domsf |
PANTHERi | PTHR22738, PTHR22738, 1 hit PTHR22738:SF12, PTHR22738:SF12, 1 hit |
Pfami | View protein in Pfam PF00130, C1_1, 1 hit PF16517, Nore1-SARAH, 1 hit PF00788, RA, 1 hit |
SMARTi | View protein in SMART SM00109, C1, 1 hit SM00314, RA, 1 hit |
SUPFAMi | SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS50200, RA, 1 hit PS50951, SARAH, 1 hit PS00479, ZF_DAG_PE_1, 1 hit PS50081, ZF_DAG_PE_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative promoter usage. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSAEPELIEL RELAPSGRIG PGRTRLERAN ALRIAPGTTR NPSQQHVPGR
60 70 80 90 100
GHRFQPAGPT THTWCDLCGD FIWGVVRKGL QCAHCKFTCH YRCRALVCLD
110 120 130 140 150
CCGPRDLGWD SALERDTNVD EAVERETPDL SQAETEQKIK DYNGQINSNL
160 170 180 190 200
FMSLNKDGSY TGFIKVQLKL VRPVSVPSSK KPPSLQDARR GTGRSTAVKR
210 220 230 240 250
RTSFYLPKDA IKHLHVLSRT RAREVIEALL RKFMVVDDPR KFALFERTER
260 270 280 290 300
HGQVYLRKLS DDEQPLKLRL LAGPSEKALS FVLKENDSGE VNWDAFSMPE
310 320 330 340
LHNFLRILQR EEEEHLRQIL QKYSRCRQKI QEALHACPLG
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketB0QZV7 | B0QZV7_MOUSE | Ras association domain-containing p... | Rassf1 | 344 | Annotation score: | ||
D6REH3 | D6REH3_MOUSE | Ras association domain-containing p... | Rassf1 | 84 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_050779 | 1 – 70 | Missing in isoform C. 2 PublicationsAdd BLAST | 70 | |
Alternative sequenceiVSP_050780 | 71 – 119 | FIWGV…RDTNV → MGEAETPSFEMTWSSTTSSG YCSQEDSDSELEQYFTARTS LVRRPRRDQ in isoform C. 3 PublicationsAdd BLAST | 49 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF132851 mRNA Translation: AAD30061.1 AF333027 Genomic DNA Translation: AAK21200.1 AF333027 Genomic DNA Translation: AAK21201.1 BC002173 mRNA Translation: AAH02173.1 |
CCDSi | CCDS23494.1 [Q99MK9-2] CCDS57699.1 [Q99MK9-1] |
RefSeqi | NP_001230677.1, NM_001243748.1 [Q99MK9-1] NP_062687.1, NM_019713.4 [Q99MK9-2] |
Genome annotation databases
Ensembli | ENSMUST00000010211; ENSMUSP00000010211; ENSMUSG00000010067 [Q99MK9-2] ENSMUST00000093786; ENSMUSP00000091301; ENSMUSG00000010067 [Q99MK9-1] |
GeneIDi | 56289 |
KEGGi | mmu:56289 |
UCSCi | uc009rlq.2, mouse [Q99MK9-2] uc012hag.2, mouse [Q99MK9-1] |
Keywords - Coding sequence diversityi
Alternative promoter usageSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF132851 mRNA Translation: AAD30061.1 AF333027 Genomic DNA Translation: AAK21200.1 AF333027 Genomic DNA Translation: AAK21201.1 BC002173 mRNA Translation: AAH02173.1 |
CCDSi | CCDS23494.1 [Q99MK9-2] CCDS57699.1 [Q99MK9-1] |
RefSeqi | NP_001230677.1, NM_001243748.1 [Q99MK9-1] NP_062687.1, NM_019713.4 [Q99MK9-2] |
3D structure databases
SMRi | Q99MK9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 207877, 32 interactors |
IntActi | Q99MK9, 31 interactors |
MINTi | Q99MK9 |
STRINGi | 10090.ENSMUSP00000010211 |
PTM databases
iPTMneti | Q99MK9 |
PhosphoSitePlusi | Q99MK9 |
Proteomic databases
EPDi | Q99MK9 |
PaxDbi | Q99MK9 |
PRIDEi | Q99MK9 |
ProteomicsDBi | 255104 [Q99MK9-1] 255105 [Q99MK9-2] |
Protocols and materials databases
Antibodypediai | 30900, 516 antibodies |
DNASUi | 56289 |
Genome annotation databases
Ensembli | ENSMUST00000010211; ENSMUSP00000010211; ENSMUSG00000010067 [Q99MK9-2] ENSMUST00000093786; ENSMUSP00000091301; ENSMUSG00000010067 [Q99MK9-1] |
GeneIDi | 56289 |
KEGGi | mmu:56289 |
UCSCi | uc009rlq.2, mouse [Q99MK9-2] uc012hag.2, mouse [Q99MK9-1] |
Organism-specific databases
CTDi | 11186 |
MGIi | MGI:1928386, Rassf1 |
Phylogenomic databases
eggNOGi | KOG4239, Eukaryota |
GeneTreei | ENSGT00940000155664 |
HOGENOMi | CLU_045544_0_1_1 |
InParanoidi | Q99MK9 |
OMAi | GTPSFEM |
PhylomeDBi | Q99MK9 |
TreeFami | TF319243 |
Miscellaneous databases
BioGRID-ORCSi | 56289, 0 hits in 52 CRISPR screens |
ChiTaRSi | Rassf1, mouse |
PROi | PR:Q99MK9 |
RNActi | Q99MK9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000010067, Expressed in optic fissure and 298 other tissues |
ExpressionAtlasi | Q99MK9, baseline and differential |
Genevisiblei | Q99MK9, MM |
Family and domain databases
CDDi | cd00029, C1, 1 hit |
InterProi | View protein in InterPro IPR033614, C-RASSF IPR002219, PE/DAG-bd IPR000159, RA_dom IPR033600, RASSF1 IPR011524, SARAH_dom IPR029071, Ubiquitin-like_domsf |
PANTHERi | PTHR22738, PTHR22738, 1 hit PTHR22738:SF12, PTHR22738:SF12, 1 hit |
Pfami | View protein in Pfam PF00130, C1_1, 1 hit PF16517, Nore1-SARAH, 1 hit PF00788, RA, 1 hit |
SMARTi | View protein in SMART SM00109, C1, 1 hit SM00314, RA, 1 hit |
SUPFAMi | SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS50200, RA, 1 hit PS50951, SARAH, 1 hit PS00479, ZF_DAG_PE_1, 1 hit PS50081, ZF_DAG_PE_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RASF1_MOUSE | |
Accessioni | Q99MK9Primary (citable) accession number: Q99MK9 Secondary accession number(s): Q9WUF5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2004 |
Last sequence update: | June 1, 2001 | |
Last modified: | April 7, 2021 | |
This is version 148 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot