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UniProtKB - Q99MI6 (GIMA3_MOUSE)
Protein
GTPase IMAP family member 3
Gene
Gimap3
Organism
Mus musculus (Mouse)
Status
Functioni
During thymocyte development, may support the positive selection of CD4 and CD8 T cells (PubMed:16509771).
May play a role in mitochondrial DNA segregation in hematopoietic tissues (PubMed:25808953).
Binds GTP (PubMed:11238997).
3 PublicationsSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 50 | GTPBy similarity | 1 | |
Binding sitei | 184 | GTPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 29 – 37 | GTPBy similarity | 9 | |
Nucleotide bindingi | 147 – 149 | GTPBy similarity | 3 |
GO - Molecular functioni
- GTP binding Source: MGI
GO - Biological processi
- mitochondrial genome maintenance Source: MGI
- myeloid dendritic cell differentiation Source: MGI
- negative regulation of apoptotic process Source: MGI
- negative regulation of interferon-gamma production Source: MGI
- negative regulation of lipid catabolic process Source: MGI
- negative regulation of nitric oxide biosynthetic process Source: MGI
- negative regulation of T cell activation Source: MGI
- positive regulation of calcium ion transport into cytosol Source: MGI
- positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation Source: MGI
- positive regulation of gamma-delta T cell differentiation Source: MGI
- positive regulation of humoral immune response mediated by circulating immunoglobulin Source: MGI
- positive regulation of membrane potential Source: MGI
- positive regulation of natural killer cell cytokine production Source: MGI
- positive regulation of natural killer cell mediated cytotoxicity Source: MGI
- regulation of mitochondrial membrane permeability Source: MGI
- T cell homeostasis Source: MGI
- temperature homeostasis Source: MGI
Keywordsi
Ligand | GTP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: GTPase IMAP family member 3Alternative name(s): Immunity-associated nucleotide 4 protein Short name: IAN-41 Publication |
Gene namesi | Name:Gimap3 Synonyms:Ian42 Publications |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1932723, Gimap3 |
VEuPathDBi | HostDB:ENSMUSG00000039264 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication; Single-pass type IV membrane protein 1 Publication
Note: The mitochondrial localization originally reported was observed with C-terminally tagged protein and was not confirmed in later publications.2 Publications
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Mitochondrion
- mitochondrial outer membrane Source: MGI
- mitochondrion Source: MGI
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 279 | CytoplasmicSequence analysisAdd BLAST | 279 | |
Transmembranei | 280 – 300 | Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST | 21 | |
Topological domaini | 301 | LumenalSequence analysis | 1 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000190994 | 1 – 301 | GTPase IMAP family member 3Add BLAST | 301 |
Proteomic databases
EPDi | Q99MI6 |
MaxQBi | Q99MI6 |
PaxDbi | Q99MI6 |
PRIDEi | Q99MI6 |
ProteomicsDBi | 267794 |
Expressioni
Tissue specificityi
Expressed in thymus (in thymocytes), spleen (in splenocytes), lymph node and, at lower levels, in lung (PubMed:16509771, PubMed:25808953). Highly expressed in T lymphocytes (PubMed:16509771).2 Publications
Developmental stagei
Up-regulated upon the maturation of CD4/CD8 double-positive to CD4 single-positive thymocytes.1 Publication
Inductioni
By BCR-ABL oncogene.
Gene expression databases
Bgeei | ENSMUSG00000039264, Expressed in blood and 83 other tissues |
Genevisiblei | Q99MI6, MM |
Interactioni
Subunit structurei
Binary interactionsi
Q99MI6
With | #Exp. | IntAct |
---|---|---|
Bcl2 [P10417] | 4 | EBI-15572304,EBI-526314 |
BAX [Q07812] from Homo sapiens. | 2 | EBI-15572304,EBI-516580 |
BCL2L1 - isoform Bcl-X(L) [Q07817-1] from Homo sapiens. | 3 | EBI-15572304,EBI-287195 |
Protein-protein interaction databases
BioGRIDi | 219916, 3 interactors |
DIPi | DIP-29182N |
IntActi | Q99MI6, 6 interactors |
STRINGi | 10090.ENSMUSP00000047435 |
Miscellaneous databases
RNActi | Q99MI6, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 20 – 223 | AIG1-type GPROSITE-ProRule annotationAdd BLAST | 204 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 263 – 301 | Required for targeting to the endoplasmic reticulum1 PublicationAdd BLAST | 39 |
Sequence similaritiesi
Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502RB0C, Eukaryota |
GeneTreei | ENSGT00940000154844 |
HOGENOMi | CLU_010468_1_1_1 |
InParanoidi | Q99MI6 |
OMAi | LHKEICA |
OrthoDBi | 1092873at2759 |
PhylomeDBi | Q99MI6 |
TreeFami | TF330845 |
Family and domain databases
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR006703, G_AIG1 IPR045058, GIMA/IAN/Toc IPR027417, P-loop_NTPase |
PANTHERi | PTHR10903, PTHR10903, 1 hit |
Pfami | View protein in Pfam PF04548, AIG1, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51720, G_AIG1, 1 hit |
i Sequence
Sequence statusi: Complete.
Q99MI6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
METLQNVVTG GKKGGCTSGS RPLRILLVGK SGCGKSATGN SLLRRPAFES
60 70 80 90 100
RLRGQSVTRT SQAETGTWEG RSILVVDTPP IFESKAQNQD MDKDIGDCYL
110 120 130 140 150
LCAPGPHVLL LVTQLGRFTA EDVMAVRMVK EVFGVGVMRH MIVLFTRKED
160 170 180 190 200
LAEKSLEEFV THTDNRSLRS LVQECGRRYC AFNNRASGEE QQGQLAELMA
210 220 230 240 250
LVRRLEQECE GSFHSNDLFL HAETLLREGY SVHQEAYRCY LAKVRQEVEK
260 270 280 290 300
QRWELEEQEG SWVLKVLPIG KKLEVLHSDF CWYLVLAILI FFVFFFLLFY
V
Sequence cautioni
The sequence AAK31138 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 99 | Y → C in AAH94914 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 209 | C → R in AAH94914 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 257 | E → G in BAC25217 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF337052 mRNA Translation: AAK31138.1 Frameshift. AB164418 mRNA Translation: BAD14959.1 BC094914 mRNA Translation: AAH94914.1 AK008376 mRNA Translation: BAC25217.1 |
CCDSi | CCDS20115.1 |
RefSeqi | NP_112537.2, NM_031247.3 |
Genome annotation databases
Ensembli | ENSMUST00000038811; ENSMUSP00000047435; ENSMUSG00000039264 ENSMUST00000204036; ENSMUSP00000145211; ENSMUSG00000039264 |
GeneIDi | 83408 |
KEGGi | mmu:83408 |
UCSCi | uc009bvt.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF337052 mRNA Translation: AAK31138.1 Frameshift. AB164418 mRNA Translation: BAD14959.1 BC094914 mRNA Translation: AAH94914.1 AK008376 mRNA Translation: BAC25217.1 |
CCDSi | CCDS20115.1 |
RefSeqi | NP_112537.2, NM_031247.3 |
3D structure databases
AlphaFoldDBi | Q99MI6 |
SMRi | Q99MI6 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 219916, 3 interactors |
DIPi | DIP-29182N |
IntActi | Q99MI6, 6 interactors |
STRINGi | 10090.ENSMUSP00000047435 |
Proteomic databases
EPDi | Q99MI6 |
MaxQBi | Q99MI6 |
PaxDbi | Q99MI6 |
PRIDEi | Q99MI6 |
ProteomicsDBi | 267794 |
Protocols and materials databases
DNASUi | 83408 |
Genome annotation databases
Ensembli | ENSMUST00000038811; ENSMUSP00000047435; ENSMUSG00000039264 ENSMUST00000204036; ENSMUSP00000145211; ENSMUSG00000039264 |
GeneIDi | 83408 |
KEGGi | mmu:83408 |
UCSCi | uc009bvt.1, mouse |
Organism-specific databases
CTDi | 83408 |
MGIi | MGI:1932723, Gimap3 |
VEuPathDBi | HostDB:ENSMUSG00000039264 |
Phylogenomic databases
eggNOGi | ENOG502RB0C, Eukaryota |
GeneTreei | ENSGT00940000154844 |
HOGENOMi | CLU_010468_1_1_1 |
InParanoidi | Q99MI6 |
OMAi | LHKEICA |
OrthoDBi | 1092873at2759 |
PhylomeDBi | Q99MI6 |
TreeFami | TF330845 |
Miscellaneous databases
BioGRID-ORCSi | 83408, 3 hits in 72 CRISPR screens |
ChiTaRSi | Gimap3, mouse |
PROi | PR:Q99MI6 |
RNActi | Q99MI6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000039264, Expressed in blood and 83 other tissues |
Genevisiblei | Q99MI6, MM |
Family and domain databases
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR006703, G_AIG1 IPR045058, GIMA/IAN/Toc IPR027417, P-loop_NTPase |
PANTHERi | PTHR10903, PTHR10903, 1 hit |
Pfami | View protein in Pfam PF04548, AIG1, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51720, G_AIG1, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GIMA3_MOUSE | |
Accessioni | Q99MI6Primary (citable) accession number: Q99MI6 Secondary accession number(s): Q4VBX0, Q549W4, Q8CF04 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 14, 2003 |
Last sequence update: | November 14, 2003 | |
Last modified: | May 25, 2022 | |
This is version 135 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families