We will be switching to the new UniProt website soon. Please explore and share your feedback.
Take me to the new website.
UniProtKB - Q99MA9 (NKX61_MOUSE)
Protein
Homeobox protein Nkx-6.1
Gene
Nkx6-1
Organism
Mus musculus (Mouse)
Status
Functioni
Transcription factor which binds to specific A/T-rich DNA sequences in the promoter regions of a number of genes. Required for the development of insulin-producing beta cells in the islets of Langerhans at the secondary transition (PubMed:11076772).
Involved in transcriptional regulation of the insulin gene. Together with NKX2-2 and IRX3, restricts the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class II proteins of neuronal progenitor factors, which are induced by SHH signals.
2 PublicationsRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 237 – 296 | HomeoboxPROSITE-ProRule annotationAdd BLAST | 60 |
GO - Molecular functioni
- chromatin binding Source: MGI
- DNA-binding transcription factor activity Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: MGI
- sequence-specific DNA binding Source: MGI
GO - Biological processi
- cell differentiation Source: MGI
- cell population proliferation Source: MGI
- cellular response to cytokine stimulus Source: Ensembl
- cellular response to peptide hormone stimulus Source: Ensembl
- central nervous system neuron differentiation Source: MGI
- endocrine pancreas development Source: MGI
- negative regulation of glial cell differentiation Source: MGI
- negative regulation of transcription by RNA polymerase II Source: MGI
- neurogenesis Source: MGI
- neuron migration Source: InterPro
- oligodendrocyte differentiation Source: MGI
- positive regulation of glial cell differentiation Source: MGI
- positive regulation of insulin secretion Source: MGI
- positive regulation of neuron differentiation Source: MGI
- regulation of axon extension Source: MGI
- regulation of neuron migration Source: MGI
- regulation of transcription by RNA polymerase II Source: MGI
- response to nicotine Source: Ensembl
- response to xenobiotic stimulus Source: Ensembl
- smoothened signaling pathway Source: MGI
- transcription by RNA polymerase II Source: MGI
- type B pancreatic cell development Source: Ensembl
- type B pancreatic cell proliferation Source: UniProtKB
Keywordsi
Molecular function | Developmental protein, DNA-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Homeobox protein Nkx-6.1Alternative name(s): Homeobox protein NK-6 homolog A |
Gene namesi | Name:Nkx6-1 Synonyms:Nkx6.1, Nkx6a |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1206039, Nkx6-1 |
VEuPathDBi | HostDB:ENSMUSG00000035187 |
Pathology & Biotechi
Disruption phenotypei
Embryonic development of mutant mice is normal until 13.5 dpc. At this stage, the normal expansion of pancreatic beta cells does not occur. At 18.5 dpc, the numbers of beta cells is dramatically decreased and the pancreatic insulin content is only 2% of wild-type. No effect on the production of other pancreatic hormones, including glucagon, somatostatin and pancreatic polypeptide (PP) (PubMed:11076772). Simultaneous knockout of NKX6-1 and NKX2-2 results in the complete absence of insulin-expressing cells in the pancreas throughout development, and instead accumulation of incompletely differentiated beta cells, a phenotype not distinguable from the single NKX2-2 knockout (PubMed:11076772).1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000048953 | 1 – 365 | Homeobox protein Nkx-6.1Add BLAST | 365 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 190 | Asymmetric dimethylarginineBy similarity | 1 |
Keywords - PTMi
MethylationProteomic databases
PaxDbi | Q99MA9 |
PRIDEi | Q99MA9 |
ProteomicsDBi | 293855 |
PTM databases
iPTMneti | Q99MA9 |
PhosphoSitePlusi | Q99MA9 |
Expressioni
Tissue specificityi
Expressed by neuronal progenitor cells in discrete domains of the ventral neural tube (PubMed:10830170). In the pancreas, expressed exclusively in insulin-producing beta cells of the islets of Langerhans (at protein level) (PubMed:11076772).2 Publications
Developmental stagei
In the developing pancreas, detected as early as 10.5 dpc in the majority of epithelial cells. This broad expression pattern persists through 12.5 dpc. Around 13.5 dpc, with the start of the secondary transition, becomes restricted and by 15.5 dpc, exclusively detected in insulin-expressing beta cells and in some scattered ductal and periductal cells (at protein level).1 Publication
Gene expression databases
Bgeei | ENSMUSG00000035187, Expressed in dorsal pancreas and 93 other tissues |
Genevisiblei | Q99MA9, MM |
Interactioni
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000042716 |
Miscellaneous databases
RNActi | Q99MA9, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 35 – 136 | DisorderedSequence analysisAdd BLAST | 102 | |
Regioni | 102 – 269 | Repressor domainBy similarityAdd BLAST | 168 | |
Regioni | 295 – 365 | DisorderedSequence analysisAdd BLAST | 71 | |
Regioni | 307 – 365 | Involved in DNA-bindingBy similarityAdd BLAST | 59 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 49 – 65 | Polar residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 82 – 96 | Polar residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 116 – 136 | Polar residuesSequence analysisAdd BLAST | 21 | |
Compositional biasi | 301 – 322 | Basic and acidic residuesSequence analysisAdd BLAST | 22 |
Domaini
The C-terminal domain contributes to sequence-specific DNA-binding.
Keywords - Domaini
HomeoboxPhylogenomic databases
eggNOGi | KOG0847, Eukaryota |
GeneTreei | ENSGT00940000160897 |
HOGENOMi | CLU_064820_0_0_1 |
InParanoidi | Q99MA9 |
OMAi | VGAMEGP |
OrthoDBi | 1263401at2759 |
PhylomeDBi | Q99MA9 |
TreeFami | TF327063 |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR017970, Homeobox_CS IPR001356, Homeobox_dom IPR020479, Homeobox_metazoa IPR000047, HTH_motif IPR033630, NKX-6.1 |
PANTHERi | PTHR24340:SF31, PTHR24340:SF31, 1 hit |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit |
PRINTSi | PR00024, HOMEOBOX PR00031, HTHREPRESSR |
SMARTi | View protein in SMART SM00389, HOX, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit |
PROSITEi | View protein in PROSITE PS00027, HOMEOBOX_1, 1 hit PS50071, HOMEOBOX_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q99MA9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLAVGAMEGP RQSAFLLSSP PLAALHSMAE MKTPLYPAAY PPLPTGPPSS
60 70 80 90 100
SSSSSSSSSP SPPLGSHNPG GLKPPAAGGL SSLGSPPQQL SAATPHGIND
110 120 130 140 150
ILSRPSMPVA SGAALPSASP SGSSSSSSSS ASATSASAAA AAAAAAAAAA
160 170 180 190 200
ASSPAGLLAG LPRFSSLSPP PPPPGLYFSP SAAAVAAVGR YPKPLAELPG
210 220 230 240 250
RTPIFWPGVM QSPPWRDARL ACTPHQGSIL LDKDGKRKHT RPTFSGQQIF
260 270 280 290 300
ALEKTFEQTK YLAGPERARL AYSLGMTESQ VKVWFQNRRT KWRKKHAAEM
310 320 330 340 350
ATAKKKQDSE TERLKGTSEN EEDDDDYNKP LDPNSDDEKI TQLLKKHKSS
360
GGSLLLHASE AEGSS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF357883 mRNA Translation: AAK37567.1 BC138019 mRNA Translation: AAI38020.1 BC138020 mRNA Translation: AAI38021.1 AF291666 Genomic DNA Translation: AAG30415.1 |
CCDSi | CCDS19471.1 |
RefSeqi | NP_659204.1, NM_144955.2 |
Genome annotation databases
Ensembli | ENSMUST00000044125; ENSMUSP00000042716; ENSMUSG00000035187 |
GeneIDi | 18096 |
KEGGi | mmu:18096 |
UCSCi | uc008yim.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF357883 mRNA Translation: AAK37567.1 BC138019 mRNA Translation: AAI38020.1 BC138020 mRNA Translation: AAI38021.1 AF291666 Genomic DNA Translation: AAG30415.1 |
CCDSi | CCDS19471.1 |
RefSeqi | NP_659204.1, NM_144955.2 |
3D structure databases
SMRi | Q99MA9 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000042716 |
PTM databases
iPTMneti | Q99MA9 |
PhosphoSitePlusi | Q99MA9 |
Proteomic databases
PaxDbi | Q99MA9 |
PRIDEi | Q99MA9 |
ProteomicsDBi | 293855 |
Protocols and materials databases
Antibodypediai | 25238, 341 antibodies from 31 providers |
DNASUi | 18096 |
Genome annotation databases
Ensembli | ENSMUST00000044125; ENSMUSP00000042716; ENSMUSG00000035187 |
GeneIDi | 18096 |
KEGGi | mmu:18096 |
UCSCi | uc008yim.1, mouse |
Organism-specific databases
CTDi | 4825 |
MGIi | MGI:1206039, Nkx6-1 |
VEuPathDBi | HostDB:ENSMUSG00000035187 |
Phylogenomic databases
eggNOGi | KOG0847, Eukaryota |
GeneTreei | ENSGT00940000160897 |
HOGENOMi | CLU_064820_0_0_1 |
InParanoidi | Q99MA9 |
OMAi | VGAMEGP |
OrthoDBi | 1263401at2759 |
PhylomeDBi | Q99MA9 |
TreeFami | TF327063 |
Miscellaneous databases
BioGRID-ORCSi | 18096, 0 hits in 63 CRISPR screens |
PROi | PR:Q99MA9 |
RNActi | Q99MA9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000035187, Expressed in dorsal pancreas and 93 other tissues |
Genevisiblei | Q99MA9, MM |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR017970, Homeobox_CS IPR001356, Homeobox_dom IPR020479, Homeobox_metazoa IPR000047, HTH_motif IPR033630, NKX-6.1 |
PANTHERi | PTHR24340:SF31, PTHR24340:SF31, 1 hit |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit |
PRINTSi | PR00024, HOMEOBOX PR00031, HTHREPRESSR |
SMARTi | View protein in SMART SM00389, HOX, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit |
PROSITEi | View protein in PROSITE PS00027, HOMEOBOX_1, 1 hit PS50071, HOMEOBOX_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NKX61_MOUSE | |
Accessioni | Q99MA9Primary (citable) accession number: Q99MA9 Secondary accession number(s): B2RQP4, Q9ERQ7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 10, 2003 |
Last sequence update: | June 1, 2001 | |
Last modified: | February 23, 2022 | |
This is version 151 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot