Homeobox protein Nkx-6.1

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functionⁱ

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functionⁱ

• chromatin binding Source: MGI
• DNA-binding transcription factor activity Source: MGI
• DNA-binding transcription repressor activity, RNA polymerase II-specific Source: MGI
• RNA polymerase II proximal promoter sequence-specific DNA binding Source: MGI
• sequence-specific DNA binding Source: MGI

GO - Biological processⁱ

• cell differentiation Source: MGI <p>Inferred from Mutant Phenotype</p> <p>Describes annotations that are concluded from looking at variations or changes in a gene product such as mutations or abnormal levels and includes techniques such as knockouts, overexpression, anti-sense experiments and use of specific protein inhibitors.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#imp">GO evidence code guide</a></p> Inferred from mutant phenotypeⁱ
  • "NKX6 transcription factor activity is required for alpha- and beta-cell development in the pancreas."
    Henseleit K.D., Nelson S.B., Kuhlbrodt K., Hennings J.C., Ericson J., Sander M.
    Development 132:3139-3149(2005) [PubMed] [Europe PMC] [Abstract]
• cell population proliferation Source: MGI
• cellular response to cytokine stimulus Source: Ensembl
• cellular response to peptide hormone stimulus Source: Ensembl
• central nervous system neuron differentiation Source: MGIInferred from mutant phenotypeⁱ
  • "Nkx6-1 controls the identity and fate of red nucleus and oculomotor neurons in the mouse midbrain."
    Prakash N., Puelles E., Freude K., Trumbach D., Omodei D., Di Salvio M., Sussel L., Ericson J., Sander M., Simeone A., Wurst W.
    Development 136:2545-2555(2009) [PubMed] [Europe PMC] [Abstract]
• endocrine pancreas development Source: MGIInferred from mutant phenotypeⁱ
  • "NKX6 transcription factor activity is required for alpha- and beta-cell development in the pancreas."
    Henseleit K.D., Nelson S.B., Kuhlbrodt K., Hennings J.C., Ericson J., Sander M.
    Development 132:3139-3149(2005) [PubMed] [Europe PMC] [Abstract]
• negative regulation of glial cell differentiation Source: MGI <p>Inferred from Genetic Interaction</p> <p>Used to describe “traditional” genetic interactions such as suppressors and synthetic lethals as well as other techniques such as functional complementation, rescue experiments, or inferences about a gene drawn from the phenotype of a mutation in a different gene.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#igi">GO evidence code guide</a></p> Inferred from genetic interactionⁱ
  • "Multiple dorsoventral origins of oligodendrocyte generation in the spinal cord and hindbrain."
    Vallstedt A., Klos J.M., Ericson J.
    Neuron 45:55-67(2005) [PubMed] [Europe PMC] [Abstract]
• negative regulation of transcription by RNA polymerase II Source: MGI
• neurogenesis Source: MGI
• neuron migration Source: InterPro
• oligodendrocyte differentiation Source: MGIInferred from genetic interactionⁱ
  • "Generation of oligodendrocyte precursor cells from mouse dorsal spinal cord independent of Nkx6 regulation and Shh signaling."
    Cai J., Qi Y., Hu X., Tan M., Liu Z., Zhang J., Li Q., Sander M., Qiu M.
    Neuron 45:41-53(2005) [PubMed] [Europe PMC] [Abstract]
• positive regulation of glial cell differentiation Source: MGIInferred from genetic interactionⁱ
  • "Multiple dorsoventral origins of oligodendrocyte generation in the spinal cord and hindbrain."
    Vallstedt A., Klos J.M., Ericson J.
    Neuron 45:55-67(2005) [PubMed] [Europe PMC] [Abstract]
• positive regulation of insulin secretion Source: MGI
• positive regulation of neuron differentiation Source: MGI <p>Inferred from Direct Assay</p> <p>Used to indicate a direct assay for the function, process or component indicated by the GO term.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#ida">GO evidence code guide</a></p> Inferred from direct assayⁱ
  • Ref.5

    "A homeodomain protein code specifies progenitor cell identity and neuronal fate in the ventral neural tube."
    Briscoe J., Pierani A., Jessell T.M., Ericson J.
    Cell 101:435-445(2000) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION IN NEURAL PATTERNING, TISSUE SPECIFICITY.
• positive regulation of transcription by RNA polymerase II Source: GO_Central <p>Inferred from Biological aspect of Ancestor</p> <p>A type of phylogenetic evidence whereby an aspect of a descendent is inferred through the characterization of an aspect of a ancestral gene.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#iba">GO evidence code guide</a></p> Inferred from biological aspect of ancestorⁱ
  • "Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium."
    Gaudet P., Livstone M.S., Lewis S.E., Thomas P.D.
    Brief. Bioinformatics 12:449-462(2011) [PubMed] [Europe PMC] [Abstract]
• regulation of axon extension Source: MGIInferred from mutant phenotypeⁱ
  • "Nkx6-1 controls the identity and fate of red nucleus and oculomotor neurons in the mouse midbrain."
    Prakash N., Puelles E., Freude K., Trumbach D., Omodei D., Di Salvio M., Sussel L., Ericson J., Sander M., Simeone A., Wurst W.
    Development 136:2545-2555(2009) [PubMed] [Europe PMC] [Abstract]
• regulation of neuron migration Source: MGIInferred from mutant phenotypeⁱ
  • "Nkx6-1 controls the identity and fate of red nucleus and oculomotor neurons in the mouse midbrain."
    Prakash N., Puelles E., Freude K., Trumbach D., Omodei D., Di Salvio M., Sussel L., Ericson J., Sander M., Simeone A., Wurst W.
    Development 136:2545-2555(2009) [PubMed] [Europe PMC] [Abstract]
• regulation of transcription by RNA polymerase II Source: MGIInferred from mutant phenotypeⁱ
  • "Domain-specific control of neurogenesis achieved through patterned regulation of Notch ligand expression."
    Marklund U., Hansson E.M., Sundstroem E., de Angelis M.H., Przemeck G.K., Lendahl U., Muhr J., Ericson J.
    Development 137:437-445(2010) [PubMed] [Europe PMC] [Abstract]
• regulation of transcription from RNA polymerase II promoter involved in spinal cord motor neuron fate specification Source: MGIInferred from genetic interactionⁱ
  • "Different levels of repressor activity assign redundant and specific roles to Nkx6 genes in motor neuron and interneuron specification."
    Vallstedt A., Muhr J., Pattyn A., Pierani A., Mendelsohn M., Sander M., Jessell T.M., Ericson J.
    Neuron 31:743-755(2001) [PubMed] [Europe PMC] [Abstract]
• regulation of transcription from RNA polymerase II promoter involved in ventral spinal cord interneuron specification Source: MGIInferred from mutant phenotypeⁱ
  • "Different levels of repressor activity assign redundant and specific roles to Nkx6 genes in motor neuron and interneuron specification."
    Vallstedt A., Muhr J., Pattyn A., Pierani A., Mendelsohn M., Sander M., Jessell T.M., Ericson J.
    Neuron 31:743-755(2001) [PubMed] [Europe PMC] [Abstract]
• response to nicotine Source: Ensembl
• smoothened signaling pathway Source: MGIInferred from direct assayⁱ
  • "FKBP8 cell-autonomously controls neural tube patterning through a Gli2- and Kif3a-dependent mechanism."
    Cho A., Ko H.W., Eggenschwiler J.T.
    Dev. Biol. 321:27-39(2008) [PubMed] [Europe PMC] [Abstract]
• transcription by RNA polymerase II Source: MGI
• type B pancreatic cell development Source: Ensembl
• type B pancreatic cell proliferation Source: UniProtKB

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsⁱ

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyⁱ

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationⁱ

Keywords - Cellular componentⁱ

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechⁱ

<p>This subsection of the ‘Pathology and Biotech’ section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypeⁱ

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingⁱ

Molecule processing

Amino acid modifications

Keywords - PTMⁱ

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressionⁱ

<p>This subsection of the ‘Expression’ section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified ‘at protein level’. <br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityⁱ

<p>This subsection of the ‘Expression’ section provides information on the expression of the gene product at various stages of a cell, tissue or organism development. By default, the information is derived from experiments at the mRNA level, unless specified ‘at the protein level’.<p><a href='/help/developmental_stage' target='_top'>More...</a></p>Developmental stageⁱ

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactionⁱ

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structureⁱ

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsⁱ

Region

Compositional bias

<p>This subsection of the ‘Family and domains’ section provides general information on the biological role of a domain. The term ‘domain’ is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domainⁱ

Keywords - Domainⁱ

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequenceⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusⁱ: Complete.

        10         20         30         40         50
MLAVGAMEGP RQSAFLLSSP PLAALHSMAE MKTPLYPAAY PPLPTGPPSS 
        60         70         80         90        100
SSSSSSSSSP SPPLGSHNPG GLKPPAAGGL SSLGSPPQQL SAATPHGIND 
       110        120        130        140        150
ILSRPSMPVA SGAALPSASP SGSSSSSSSS ASATSASAAA AAAAAAAAAA 
       160        170        180        190        200
ASSPAGLLAG LPRFSSLSPP PPPPGLYFSP SAAAVAAVGR YPKPLAELPG 
       210        220        230        240        250
RTPIFWPGVM QSPPWRDARL ACTPHQGSIL LDKDGKRKHT RPTFSGQQIF 
       260        270        280        290        300
ALEKTFEQTK YLAGPERARL AYSLGMTESQ VKVWFQNRRT KWRKKHAAEM 
       310        320        330        340        350
ATAKKKQDSE TERLKGTSEN EEDDDDYNKP LDPNSDDEKI TQLLKKHKSS 
       360 
GGSLLLHASE AEGSS                                       

Sequence databases

Genome annotation databases

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsⁱ

• 90% Identity
• 50% Identity

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesⁱ

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationⁱ

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousⁱ

Keywords - Technical termⁱ

Documents

1. MGD cross-references
   Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot