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Protein

Homeobox protein Nkx-6.1

Gene

Nkx6-1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor which binds to specific A/T-rich DNA sequences in the promoter regions of a number of genes. Required for the development of insulin-producing beta cells in the islets of Langerhans at the secondary transition (PubMed:11076772). Involved in transcriptional regulation of the insulin gene. Together with NKX2-2 and IRX3, restricts the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class II proteins of neuronal progenitor factors, which are induced by SHH signals.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi237 – 296HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Homeobox protein Nkx-6.1
Alternative name(s):
Homeobox protein NK-6 homolog A
Gene namesi
Name:Nkx6-1
Synonyms:Nkx6.1, Nkx6a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1206039 Nkx6-1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Embryonic development of mutant mice is normal until 13.5 dpc. At this stage, the normal expansion of pancreatic beta cells does not occur. At 18.5 dpc, the numbers of beta cells is dramatically decreased and the pancreatic insulin content is only 2% of wild type. No effect on the production of other pancreatic hormones, including glucagon, somatostatin and pancreatic polypeptide (PP) (PubMed:11076772). Simultaneous knockout of NKX6-1 and NKX2-2 results in the complete absence of insulin-expressing cells in the pancreas throughout development, and instead accumulation of incompletely differentiated beta cells, a phenotype not distinguable from the single NKX2-2 knockout (PubMed:11076772).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000489531 – 365Homeobox protein Nkx-6.1Add BLAST365

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei190Asymmetric dimethylarginineBy similarity1

Keywords - PTMi

Methylation

Proteomic databases

PaxDbiQ99MA9
PRIDEiQ99MA9

PTM databases

PhosphoSitePlusiQ99MA9

Expressioni

Tissue specificityi

Expressed by neuronal progenitor cells in discrete domains of the ventral neural tube (PubMed:10830170). In the pancreas, expressed exclusively in insulin-producing beta cells of the islets of Langerhans (at protein level) (PubMed:11076772).2 Publications

Developmental stagei

In the developing pancreas, detected as early as 10.5 dpc in the majority of epithelial cells. This broad expression pattern persists through 12.5 dpc. Around 13.5 dpc, with the start of the secondary transition, becomes restricted and by 15.5 dpc, exclusively detected in insulin-expressing beta cells and in some scattered ductal and periductal cells (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000035187 Expressed in 69 organ(s), highest expression level in dorsal pancreas
GenevisibleiQ99MA9 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000042716

Structurei

3D structure databases

ProteinModelPortaliQ99MA9
SMRiQ99MA9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni102 – 269Repressor domainBy similarityAdd BLAST168
Regioni307 – 365Involved in DNA-bindingBy similarityAdd BLAST59

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi49 – 61Poly-SerAdd BLAST13
Compositional biasi119 – 132Poly-SerAdd BLAST14
Compositional biasi136 – 151Poly-AlaAdd BLAST16
Compositional biasi169 – 174Poly-Pro6
Compositional biasi323 – 326Poly-Asp4

Domaini

The C-terminal domain contributes to sequence-specific DNA-binding.

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG0847 Eukaryota
ENOG410XT1X LUCA
GeneTreeiENSGT00910000143996
HOGENOMiHOG000060127
HOVERGENiHBG070987
InParanoidiQ99MA9
KOiK08030
OMAiVGAMEGP
OrthoDBiEOG091G142D
PhylomeDBiQ99MA9
TreeFamiTF327063

Family and domain databases

CDDicd00086 homeodomain, 1 hit
InterProiView protein in InterPro
IPR009057 Homeobox-like_sf
IPR017970 Homeobox_CS
IPR001356 Homeobox_dom
IPR020479 Homeobox_metazoa
IPR000047 HTH_motif
IPR033630 NKX-6.1
PANTHERiPTHR24340:SF31 PTHR24340:SF31, 1 hit
PfamiView protein in Pfam
PF00046 Homeobox, 1 hit
PRINTSiPR00024 HOMEOBOX
PR00031 HTHREPRESSR
SMARTiView protein in SMART
SM00389 HOX, 1 hit
SUPFAMiSSF46689 SSF46689, 1 hit
PROSITEiView protein in PROSITE
PS00027 HOMEOBOX_1, 1 hit
PS50071 HOMEOBOX_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q99MA9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLAVGAMEGP RQSAFLLSSP PLAALHSMAE MKTPLYPAAY PPLPTGPPSS
60 70 80 90 100
SSSSSSSSSP SPPLGSHNPG GLKPPAAGGL SSLGSPPQQL SAATPHGIND
110 120 130 140 150
ILSRPSMPVA SGAALPSASP SGSSSSSSSS ASATSASAAA AAAAAAAAAA
160 170 180 190 200
ASSPAGLLAG LPRFSSLSPP PPPPGLYFSP SAAAVAAVGR YPKPLAELPG
210 220 230 240 250
RTPIFWPGVM QSPPWRDARL ACTPHQGSIL LDKDGKRKHT RPTFSGQQIF
260 270 280 290 300
ALEKTFEQTK YLAGPERARL AYSLGMTESQ VKVWFQNRRT KWRKKHAAEM
310 320 330 340 350
ATAKKKQDSE TERLKGTSEN EEDDDDYNKP LDPNSDDEKI TQLLKKHKSS
360
GGSLLLHASE AEGSS
Length:365
Mass (Da):37,705
Last modified:June 1, 2001 - v1
Checksum:iB6AABAF5748A99E8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF357883 mRNA Translation: AAK37567.1
BC138019 mRNA Translation: AAI38020.1
BC138020 mRNA Translation: AAI38021.1
AF291666 Genomic DNA Translation: AAG30415.1
CCDSiCCDS19471.1
RefSeqiNP_659204.1, NM_144955.2
UniGeneiMm.193072

Genome annotation databases

EnsembliENSMUST00000044125; ENSMUSP00000042716; ENSMUSG00000035187
GeneIDi18096
KEGGimmu:18096
UCSCiuc008yim.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF357883 mRNA Translation: AAK37567.1
BC138019 mRNA Translation: AAI38020.1
BC138020 mRNA Translation: AAI38021.1
AF291666 Genomic DNA Translation: AAG30415.1
CCDSiCCDS19471.1
RefSeqiNP_659204.1, NM_144955.2
UniGeneiMm.193072

3D structure databases

ProteinModelPortaliQ99MA9
SMRiQ99MA9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000042716

PTM databases

PhosphoSitePlusiQ99MA9

Proteomic databases

PaxDbiQ99MA9
PRIDEiQ99MA9

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000044125; ENSMUSP00000042716; ENSMUSG00000035187
GeneIDi18096
KEGGimmu:18096
UCSCiuc008yim.1 mouse

Organism-specific databases

CTDi4825
MGIiMGI:1206039 Nkx6-1

Phylogenomic databases

eggNOGiKOG0847 Eukaryota
ENOG410XT1X LUCA
GeneTreeiENSGT00910000143996
HOGENOMiHOG000060127
HOVERGENiHBG070987
InParanoidiQ99MA9
KOiK08030
OMAiVGAMEGP
OrthoDBiEOG091G142D
PhylomeDBiQ99MA9
TreeFamiTF327063

Miscellaneous databases

PROiPR:Q99MA9
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000035187 Expressed in 69 organ(s), highest expression level in dorsal pancreas
GenevisibleiQ99MA9 MM

Family and domain databases

CDDicd00086 homeodomain, 1 hit
InterProiView protein in InterPro
IPR009057 Homeobox-like_sf
IPR017970 Homeobox_CS
IPR001356 Homeobox_dom
IPR020479 Homeobox_metazoa
IPR000047 HTH_motif
IPR033630 NKX-6.1
PANTHERiPTHR24340:SF31 PTHR24340:SF31, 1 hit
PfamiView protein in Pfam
PF00046 Homeobox, 1 hit
PRINTSiPR00024 HOMEOBOX
PR00031 HTHREPRESSR
SMARTiView protein in SMART
SM00389 HOX, 1 hit
SUPFAMiSSF46689 SSF46689, 1 hit
PROSITEiView protein in PROSITE
PS00027 HOMEOBOX_1, 1 hit
PS50071 HOMEOBOX_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNKX61_MOUSE
AccessioniPrimary (citable) accession number: Q99MA9
Secondary accession number(s): B2RQP4, Q9ERQ7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 10, 2003
Last sequence update: June 1, 2001
Last modified: October 10, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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