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UniProtKB - Q99M85 (SCRT1_MOUSE)
Protein
Transcriptional repressor scratch 1
Gene
Scrt1
Organism
Mus musculus (Mouse)
Status
Functioni
Transcriptional repressor that binds E-box motif CAGGTG (By similarity).
Appears to function downstream of proneural bHLH proteins in promoting neural differentiation.
By similarity1 PublicationRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 191 – 213 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 222 – 244 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 248 – 270 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 276 – 298 | C2H2-type 4PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 304 – 327 | C2H2-type 5; atypicalPROSITE-ProRule annotationAdd BLAST | 24 |
GO - Molecular functioni
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: MGI
- metal ion binding Source: UniProtKB-KW
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: MGI
- sequence-specific DNA binding Source: MGI
- sequence-specific double-stranded DNA binding Source: MGI
GO - Biological processi
- negative regulation of transcription by RNA polymerase II Source: MGI
- regulation of neuron migration Source: MGI
- regulation of transcription, DNA-templated Source: GO_Central
Keywordsi
Molecular function | DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Transcriptional repressor scratch 1Alternative name(s): Scratch homolog 1 zinc finger protein Short name: SCRT Short name: Scratch 1 Short name: mScrt |
Gene namesi | Name:Scrt1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2176606, Scrt1 |
VEuPathDBi | HostDB:ENSMUSG00000048385 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Nucleus
- nuclear body Source: MGI
- nucleus Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000047037 | 1 – 348 | Transcriptional repressor scratch 1Add BLAST | 348 |
Proteomic databases
MaxQBi | Q99M85 |
PaxDbi | Q99M85 |
PRIDEi | Q99M85 |
ProteomicsDBi | 256939 |
PTM databases
iPTMneti | Q99M85 |
PhosphoSitePlusi | Q99M85 |
Expressioni
Developmental stagei
Detected throughout the spinal cord except in the ventricular zone surrounding the central canal, which contains proliferating neurons, and dorsal root ganglia at 11.5 dpc. At 12.5 dpc, detected in the telencephalon, and by 14.5 dpc a distinct laminar pattern of expression was seen in regions adjacent to the ventricular zone. In the developing eye, expression was detected in the inner nuclear layer of the retina beginning at 13.5 dpc.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000048385, Expressed in cerebellum and 132 other tissues |
Genevisiblei | Q99M85, MM |
Interactioni
Subunit structurei
Interacts (via SNAG domain) with LIMD1 (via LIM domains), WTIP (via LIM domains) and AJUBA (via LIM domains).
1 PublicationProtein-protein interaction databases
STRINGi | 10090.ENSMUSP00000094093 |
Miscellaneous databases
RNActi | Q99M85, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 20 | SNAG domainBy similarityAdd BLAST | 20 | |
Regioni | 130 – 190 | DisorderedSequence analysisAdd BLAST | 61 |
Domaini
The N-terminal non zinc-finger region mediates the repressor activity.By similarity
Sequence similaritiesi
Belongs to the snail C2H2-type zinc-finger protein family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 191 – 213 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 222 – 244 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 248 – 270 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 276 – 298 | C2H2-type 4PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 304 – 327 | C2H2-type 5; atypicalPROSITE-ProRule annotationAdd BLAST | 24 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG2462, Eukaryota |
GeneTreei | ENSGT00940000154491 |
HOGENOMi | CLU_002678_42_3_1 |
InParanoidi | Q99M85 |
OMAi | RCHKAFA |
OrthoDBi | 1332418at2759 |
PhylomeDBi | Q99M85 |
TreeFami | TF315515 |
Family and domain databases
InterProi | View protein in InterPro IPR029794, SCRATCH1/2 IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
PANTHERi | PTHR24388:SF60, PTHR24388:SF60, 1 hit |
Pfami | View protein in Pfam PF00096, zf-C2H2, 4 hits |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 5 hits |
SUPFAMi | SSF57667, SSF57667, 3 hits |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 4 hits PS50157, ZINC_FINGER_C2H2_2, 4 hits |
i Sequence
Sequence statusi: Complete.
Q99M85-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPRSFLVKKV KLDTFSSADL DSSYGRARSD LGVRLQDKGY LSDYVGPASV
60 70 80 90 100
YDGDAEAALL KGPSPEPMYA AAVRGELGPA ASGSAPPPTP RPELATAAGG
110 120 130 140 150
YINGDAAVSE GYAADAFFIT DGRSRRKAAN ANAAAAPSTA SVAAPDSDAG
160 170 180 190 200
GGGGPGTRGS GSGSASRGGT RVGAGTEARA GSGATGAGGR HACGECGKTY
210 220 230 240 250
ATSSNLSRHK QTHRSLDSQL ARRCPTCGKV YVSMPAMAMH LLTHDLRHKC
260 270 280 290 300
GVCGKAFSRP WLLQGHMRSH TGEKPFGCAH CGKAFADRSN LRAHMQTHSA
310 320 330 340
FKHFQCKRCK KSFALKSYLN KHYESACFKG GASGPATPAP PQLSPVQA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY014997 mRNA Translation: AAK01468.1 BC057562 mRNA Translation: AAH57562.1 |
CCDSi | CCDS37123.1 |
RefSeqi | NP_570963.1, NM_130893.3 |
Genome annotation databases
Ensembli | ENSMUST00000096365; ENSMUSP00000094093; ENSMUSG00000048385 ENSMUST00000164703; ENSMUSP00000131152; ENSMUSG00000048385 |
GeneIDi | 170729 |
KEGGi | mmu:170729 |
UCSCi | uc007wko.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY014997 mRNA Translation: AAK01468.1 BC057562 mRNA Translation: AAH57562.1 |
CCDSi | CCDS37123.1 |
RefSeqi | NP_570963.1, NM_130893.3 |
3D structure databases
SMRi | Q99M85 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000094093 |
PTM databases
iPTMneti | Q99M85 |
PhosphoSitePlusi | Q99M85 |
Proteomic databases
MaxQBi | Q99M85 |
PaxDbi | Q99M85 |
PRIDEi | Q99M85 |
ProteomicsDBi | 256939 |
Protocols and materials databases
Antibodypediai | 59727, 29 antibodies from 13 providers |
DNASUi | 170729 |
Genome annotation databases
Ensembli | ENSMUST00000096365; ENSMUSP00000094093; ENSMUSG00000048385 ENSMUST00000164703; ENSMUSP00000131152; ENSMUSG00000048385 |
GeneIDi | 170729 |
KEGGi | mmu:170729 |
UCSCi | uc007wko.1, mouse |
Organism-specific databases
CTDi | 83482 |
MGIi | MGI:2176606, Scrt1 |
VEuPathDBi | HostDB:ENSMUSG00000048385 |
Phylogenomic databases
eggNOGi | KOG2462, Eukaryota |
GeneTreei | ENSGT00940000154491 |
HOGENOMi | CLU_002678_42_3_1 |
InParanoidi | Q99M85 |
OMAi | RCHKAFA |
OrthoDBi | 1332418at2759 |
PhylomeDBi | Q99M85 |
TreeFami | TF315515 |
Miscellaneous databases
BioGRID-ORCSi | 170729, 0 hits in 63 CRISPR screens |
ChiTaRSi | Scrt1, mouse |
PROi | PR:Q99M85 |
RNActi | Q99M85, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000048385, Expressed in cerebellum and 132 other tissues |
Genevisiblei | Q99M85, MM |
Family and domain databases
InterProi | View protein in InterPro IPR029794, SCRATCH1/2 IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
PANTHERi | PTHR24388:SF60, PTHR24388:SF60, 1 hit |
Pfami | View protein in Pfam PF00096, zf-C2H2, 4 hits |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 5 hits |
SUPFAMi | SSF57667, SSF57667, 3 hits |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 4 hits PS50157, ZINC_FINGER_C2H2_2, 4 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | SCRT1_MOUSE | |
Accessioni | Q99M85Primary (citable) accession number: Q99M85 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 13, 2004 |
Last sequence update: | June 1, 2001 | |
Last modified: | February 23, 2022 | |
This is version 137 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families