UniProtKB - Q99M75 (RTN4R_RAT)
Reticulon-4 receptor
Rtn4r
Functioni
Receptor for RTN4, OMG and MAG (PubMed:12037567, PubMed:15673660, PubMed:19420245).
Functions as receptor for the sialylated gangliosides GT1b and GM1. Besides, functions as receptor for chondroitin sulfate proteoglycans. Can also bind heparin. Intracellular signaling cascades are triggered via the coreceptor NGFR (By similarity).
Signaling mediates activation of Rho and downstream reorganization of the actin cytoskeleton (PubMed:18411262).
Mediates axonal growth inhibition (PubMed:12037567).
May play a role in regulating axon regeneration and neuronal plasticity in the adult central nervous system. Plays a role in postnatal brain development. Required for normal axon migration across the brain midline and normal formation of the corpus callosum (By similarity).
Protects motoneurons against apoptosis; protection against apoptosis is probably mediated via interaction with MAG (PubMed:26335717).
Acts in conjunction with RTN4 and LINGO1 in regulating neuronal precursor cell motility during cortical development. Like other family members, plays a role in restricting the number dendritic spines and the number of synapses that are formed during brain development (PubMed:22325200).
By similarity5 PublicationsGO - Molecular functioni
- chondroitin sulfate binding Source: UniProtKB
- ganglioside GM1 binding Source: UniProtKB
- ganglioside GT1b binding Source: UniProtKB
- heparin binding Source: UniProtKB
- neuregulin receptor activity Source: UniProtKB
- protein-containing complex binding Source: RGD
- Roundabout binding Source: GO_Central
- signaling receptor activity Source: UniProtKB
GO - Biological processi
- axon guidance Source: GO_Central
- axonogenesis Source: RGD
- cell surface receptor signaling pathway Source: UniProtKB
- corpus callosum development Source: UniProtKB
- negative chemotaxis Source: GO_Central
- negative regulation of axon extension Source: UniProtKB
- negative regulation of axonogenesis Source: RGD
- negative regulation of axon regeneration Source: UniProtKB
- negative regulation of neuron projection development Source: UniProtKB
- neuronal signal transduction Source: UniProtKB
- positive regulation of GTPase activity Source: UniProtKB
- positive regulation of Rho protein signal transduction Source: UniProtKB
Keywordsi
Molecular function | Receptor |
Names & Taxonomyi
Protein namesi | Recommended name: Reticulon-4 receptorAlternative name(s): Nogo receptor Short name: NgR Nogo-66 receptor |
Gene namesi | Name:Rtn4r Synonyms:Nogor |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 620810, Rtn4r |
Subcellular locationi
Plasma membrane
- Cell membrane 3 Publications; GPI-anchor By similarity
Other locations
- Membrane raft 1 Publication
- dendrite By similarity
- Perikaryon 1 Publication
- axon 1 Publication
Note: Detected along dendrites and axons, close to synapses, but clearly excluded from synapses.By similarity
Endoplasmic reticulum
- endoplasmic reticulum Source: Ensembl
Plasma Membrane
- anchored component of external side of plasma membrane Source: UniProtKB
- integral component of plasma membrane Source: UniProtKB
- plasma membrane Source: RGD
Other locations
- axonal growth cone Source: UniProtKB
- cell surface Source: UniProtKB
- dendritic shaft Source: UniProtKB
- glutamatergic synapse Source: SynGO
- growth cone Source: RGD
- membrane raft Source: UniProtKB
- neuron projection Source: UniProtKB
- neuronal cell body Source: UniProtKB
- perikaryon Source: UniProtKB-SubCell
- presynapse Source: SynGO
Keywords - Cellular componenti
Cell membrane, Cell projection, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 26 | Sequence analysisAdd BLAST | 26 | |
ChainiPRO_0000022259 | 27 – 447 | Reticulon-4 receptorAdd BLAST | 421 | |
PropeptideiPRO_0000022260 | 448 – 473 | Removed in mature formSequence analysisAdd BLAST | 26 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 27 ↔ 33 | Combined sources1 Publication | ||
Disulfide bondi | 31 ↔ 43 | Combined sources1 Publication | ||
Glycosylationi | 82 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 264 ↔ 287 | Combined sources1 Publication | ||
Disulfide bondi | 266 ↔ 335 | By similarity | ||
Disulfide bondi | 309 ↔ 336 | By similarity | ||
Glycosylationi | 372 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Lipidationi | 447 | GPI-anchor amidated serineSequence analysis | 1 |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, Glycoprotein, GPI-anchor, LipoproteinProteomic databases
PaxDbi | Q99M75 |
PTM databases
GlyGeni | Q99M75, 2 sites |
iPTMneti | Q99M75 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSRNOG00000030920, Expressed in frontal cortex and 21 other tissues |
Genevisiblei | Q99M75, RN |
Interactioni
Subunit structurei
Homodimer (By similarity).
Interacts with MAG (PubMed:15673660, PubMed:19420245).
Interacts with RTN4 and OMG (PubMed:15673660, PubMed:19420245).
Interacts with LINGO1 and NGFR (By similarity).
Interacts with KIAA0319L (By similarity).
Interacts with OLFM1; this inhibits interaction with LINGO1 and NGFR (By similarity).
By similarity2 PublicationsBinary interactionsi
Q99M75
With | #Exp. | IntAct |
---|---|---|
LINGO1 [Q96FE5] from Homo sapiens. | 2 | EBI-7370412,EBI-719955 |
GO - Molecular functioni
- Roundabout binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 250215, 5 interactors |
IntActi | Q99M75, 1 interactor |
MINTi | Q99M75 |
STRINGi | 10116.ENSRNOP00000041517 |
Structurei
Secondary structure
3D structure databases
SMRi | Q99M75 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q99M75 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 27 – 57 | LRRNTSequence analysisAdd BLAST | 31 | |
Repeati | 56 – 79 | LRR 1Sequence analysisAdd BLAST | 24 | |
Repeati | 80 – 103 | LRR 2Sequence analysisAdd BLAST | 24 | |
Repeati | 105 – 128 | LRR 3Sequence analysisAdd BLAST | 24 | |
Repeati | 129 – 152 | LRR 4Sequence analysisAdd BLAST | 24 | |
Repeati | 153 – 176 | LRR 5Sequence analysisAdd BLAST | 24 | |
Repeati | 178 – 200 | LRR 6Sequence analysisAdd BLAST | 23 | |
Repeati | 202 – 224 | LRR 7Sequence analysisAdd BLAST | 23 | |
Repeati | 225 – 248 | LRR 8Sequence analysisAdd BLAST | 24 | |
Repeati | 250 – 273 | LRR 9Sequence analysisAdd BLAST | 24 | |
Domaini | 260 – 310 | LRRCTSequence analysisAdd BLAST | 51 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 346 – 446 | DisorderedSequence analysisAdd BLAST | 101 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 429 – 443 | Polar residuesSequence analysisAdd BLAST | 15 |
Sequence similaritiesi
Keywords - Domaini
Leucine-rich repeat, Repeat, SignalPhylogenomic databases
eggNOGi | KOG0619, Eukaryota |
GeneTreei | ENSGT00940000160711 |
HOGENOMi | CLU_000288_18_6_1 |
InParanoidi | Q99M75 |
OMAi | MWLNLLP |
OrthoDBi | 826997at2759 |
PhylomeDBi | Q99M75 |
TreeFami | TF330080 |
Family and domain databases
Gene3Di | 3.80.10.10, 1 hit |
InterProi | View protein in InterPro IPR000483, Cys-rich_flank_reg_C IPR001611, Leu-rich_rpt IPR003591, Leu-rich_rpt_typical-subtyp IPR032675, LRR_dom_sf |
Pfami | View protein in Pfam PF13855, LRR_8, 2 hits |
SMARTi | View protein in SMART SM00369, LRR_TYP, 8 hits SM00082, LRRCT, 1 hit |
PROSITEi | View protein in PROSITE PS51450, LRR, 8 hits |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MKRASSGGSR LLAWVLWLQA WRVATPCPGA CVCYNEPKVT TSCPQQGLQA
60 70 80 90 100
VPTGIPASSQ RIFLHGNRIS YVPAASFQSC RNLTILWLHS NALAGIDAAA
110 120 130 140 150
FTGLTLLEQL DLSDNAQLRV VDPTTFRGLG HLHTLHLDRC GLQELGPGLF
160 170 180 190 200
RGLAALQYLY LQDNNLQALP DNTFRDLGNL THLFLHGNRI PSVPEHAFRG
210 220 230 240 250
LHSLDRLLLH QNHVARVHPH AFRDLGRLMT LYLFANNLSM LPAEVLVPLR
260 270 280 290 300
SLQYLRLNDN PWVCDCRARP LWAWLQKFRG SSSEVPCNLP QRLAGRDLKR
310 320 330 340 350
LAASDLEGCA VASGPFRPFQ TNQLTDEELL GLPKCCQPDA ADKASVLEPG
360 370 380 390 400
RPASAGNALK GRVPPGDTPP GNGSGPRHIN DSPFGTLPGS AEPPLTALRP
410 420 430 440 450
GGSEPPGLPT TGPRRRPGCS RKNRTRSHCR LGQAGSGSSG TGDAEGSGAL
460 470
PALACSLAPL GLALVLWTVL GPC
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 12 – 13 | LA → PT in AAK20166 (Ref. 1) Curated | 2 | |
Sequence conflicti | 43 | C → R in AAK20166 (Ref. 1) Curated | 1 | |
Sequence conflicti | 53 | T → A in AAK20166 (Ref. 1) Curated | 1 | |
Sequence conflicti | 284 | E → G in AAK20166 (Ref. 1) Curated | 1 | |
Sequence conflicti | 287 | C → S in AAK20166 (Ref. 1) Curated | 1 | |
Sequence conflicti | 303 | A → T in AAK20166 (Ref. 1) Curated | 1 | |
Sequence conflicti | 355 | A → V in AAK20166 (Ref. 1) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY028438 mRNA Translation: AAK20166.1 AF462390 mRNA Translation: AAM46772.1 |
RefSeqi | NP_446065.1, NM_053613.1 |
Genome annotation databases
Ensembli | ENSRNOT00000051037; ENSRNOP00000041517; ENSRNOG00000030920 |
GeneIDi | 113912 |
KEGGi | rno:113912 |
Similar proteinsi
Cross-referencesi
Web resourcesi
Protein Spotlight Nerve regrowth: nipped by a no-go - Issue 69 of April 2006 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY028438 mRNA Translation: AAK20166.1 AF462390 mRNA Translation: AAM46772.1 |
RefSeqi | NP_446065.1, NM_053613.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3KJ4 | X-ray | 3.10 | A/D | 27-312 | [»] | |
SMRi | Q99M75 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 250215, 5 interactors |
IntActi | Q99M75, 1 interactor |
MINTi | Q99M75 |
STRINGi | 10116.ENSRNOP00000041517 |
PTM databases
GlyGeni | Q99M75, 2 sites |
iPTMneti | Q99M75 |
Proteomic databases
PaxDbi | Q99M75 |
Protocols and materials databases
ABCDi | Q99M75, 2 sequenced antibodies |
Genome annotation databases
Ensembli | ENSRNOT00000051037; ENSRNOP00000041517; ENSRNOG00000030920 |
GeneIDi | 113912 |
KEGGi | rno:113912 |
Organism-specific databases
CTDi | 65078 |
RGDi | 620810, Rtn4r |
Phylogenomic databases
eggNOGi | KOG0619, Eukaryota |
GeneTreei | ENSGT00940000160711 |
HOGENOMi | CLU_000288_18_6_1 |
InParanoidi | Q99M75 |
OMAi | MWLNLLP |
OrthoDBi | 826997at2759 |
PhylomeDBi | Q99M75 |
TreeFami | TF330080 |
Miscellaneous databases
EvolutionaryTracei | Q99M75 |
PROi | PR:Q99M75 |
Gene expression databases
Bgeei | ENSRNOG00000030920, Expressed in frontal cortex and 21 other tissues |
Genevisiblei | Q99M75, RN |
Family and domain databases
Gene3Di | 3.80.10.10, 1 hit |
InterProi | View protein in InterPro IPR000483, Cys-rich_flank_reg_C IPR001611, Leu-rich_rpt IPR003591, Leu-rich_rpt_typical-subtyp IPR032675, LRR_dom_sf |
Pfami | View protein in Pfam PF13855, LRR_8, 2 hits |
SMARTi | View protein in SMART SM00369, LRR_TYP, 8 hits SM00082, LRRCT, 1 hit |
PROSITEi | View protein in PROSITE PS51450, LRR, 8 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | RTN4R_RAT | |
Accessioni | Q99M75Primary (citable) accession number: Q99M75 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 25, 2002 |
Last sequence update: | May 10, 2005 | |
Last modified: | February 23, 2022 | |
This is version 163 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Protein Spotlight
Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries