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Protein

Cell division cycle-associated protein 3

Gene

Cdca3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

F-box-like protein which is required for entry into mitosis. Acts by participating in E3 ligase complexes that mediate the ubiquitination and degradation of WEE1 kinase at G2/M phase (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division, Mitosis, Ubl conjugation pathway

Enzyme and pathway databases

UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Cell division cycle-associated protein 3
Alternative name(s):
Gene-rich cluster protein C8
Trigger of mitotic entry protein 1
Short name:
TOME-1
Gene namesi
Name:Cdca3
Synonyms:C8, Grcc8, Tome1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1315198 Cdca3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002877091 – 266Cell division cycle-associated protein 3Add BLAST266

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei29PhosphoserineCombined sources1
Modified residuei31PhosphoserineBy similarity1
Modified residuei37PhosphothreonineBy similarity1
Modified residuei44PhosphoserineBy similarity1
Modified residuei67PhosphoserineCombined sources1
Modified residuei75PhosphothreonineBy similarity1
Modified residuei93PhosphoserineBy similarity1
Modified residuei197PhosphoserineCombined sources1
Modified residuei200PhosphothreonineBy similarity1
Modified residuei207PhosphoserineCombined sources1
Modified residuei210PhosphothreonineCombined sources1

Post-translational modificationi

Ubiquitinated and degraded by the APC/C-Cdh1 complex.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ99M54
PaxDbiQ99M54
PeptideAtlasiQ99M54
PRIDEiQ99M54

PTM databases

iPTMnetiQ99M54
PhosphoSitePlusiQ99M54

Expressioni

Gene expression databases

BgeeiENSMUSG00000023505 Expressed in 215 organ(s), highest expression level in testis
CleanExiMM_CDCA3
ExpressionAtlasiQ99M54 baseline and differential
GenevisibleiQ99M54 MM

Interactioni

Subunit structurei

Interacts with SKP1. Part of a SCF (SKP1-cullin-F-box) protein ligase complex.1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000024270

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni90 – 119F-box-likeAdd BLAST30

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi256 – 258KEN box3

Domaini

The KEN box is required for the association with the APC/C-Cdh1 complex.By similarity

Phylogenomic databases

eggNOGiENOG410IQ4X Eukaryota
ENOG410Y54T LUCA
GeneTreeiENSGT00390000017343
HOGENOMiHOG000013100
HOVERGENiHBG053225
InParanoidiQ99M54
OMAiGHDKENQ
OrthoDBiEOG091G0MF1
PhylomeDBiQ99M54
TreeFamiTF101068

Family and domain databases

InterProiView protein in InterPro
IPR038832 CDCA3
PANTHERiPTHR34756 PTHR34756, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q99M54-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGSTQSVSGT PARPLPRNKQ VARVADPRSP SAGIQRTPIQ VESSPQPSLP
60 70 80 90 100
AEQLNGLKQA QDPDPRSPTL GIARTPMKIS GPDPQCSLVK ELSEVLETEA
110 120 130 140 150
SESISSPELA LPRETPLFYD LDLSSDPQLS PEDQLLPWSQ AELDPKQVFT
160 170 180 190 200
KEEAKQSAET IAASQNSDKP SRDPETPQSS GSKRSRRKAN SKVLGRSPLT
210 220 230 240 250
ILQDDNSPGT LTLRQGKRPS ALSENVKDLK EGVVLGTGRF LKAGGGAREP
260
NQDHDKENQH FALLES
Length:266
Mass (Da):28,708
Last modified:May 15, 2007 - v2
Checksum:i8E02E77ACAAAC2F8
GO
Isoform 2 (identifier: Q99M54-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     83-84: DP → GK
     85-266: Missing.

Note: No experimental confirmation available.
Show »
Length:84
Mass (Da):8,791
Checksum:i4D3621305F809E22
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3YXE3D3YXE3_MOUSE
Cell division cycle-associated prot...
Cdca3
180Annotation score:
D3YZ81D3YZ81_MOUSE
Cell division cycle-associated prot...
Cdca3
187Annotation score:
D3Z3H6D3Z3H6_MOUSE
Cell division cycle-associated prot...
Cdca3
213Annotation score:

Sequence cautioni

The sequence AAH27172 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti119Y → S in AAH02006 (PubMed:15489334).Curated1
Sequence conflicti126D → G in AAH02006 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_02560683 – 84DP → GK in isoform 2. 1 Publication2
Alternative sequenceiVSP_02560785 – 266Missing in isoform 2. 1 PublicationAdd BLAST182

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK008606 mRNA Translation: BAB25773.1
AK010409 mRNA Translation: BAB26916.1
AK011313 mRNA Translation: BAB27539.1
AK028159 mRNA Translation: BAC25785.1
AK145537 mRNA Translation: BAE26492.1
AK169792 mRNA Translation: BAE41370.1
AC002397 Genomic DNA Translation: AAC36014.1
BC002006 mRNA Translation: AAH02006.1
BC027172 mRNA Translation: AAH27172.1 Different initiation.
CCDSiCCDS39629.1 [Q99M54-1]
RefSeqiNP_038566.1, NM_013538.5 [Q99M54-1]
XP_006505627.1, XM_006505564.3 [Q99M54-1]
XP_017176884.1, XM_017321395.1 [Q99M54-1]
UniGeneiMm.285723

Genome annotation databases

EnsembliENSMUST00000024270; ENSMUSP00000024270; ENSMUSG00000023505 [Q99M54-1]
GeneIDi14793
KEGGimmu:14793
UCSCiuc009dsc.2 mouse [Q99M54-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK008606 mRNA Translation: BAB25773.1
AK010409 mRNA Translation: BAB26916.1
AK011313 mRNA Translation: BAB27539.1
AK028159 mRNA Translation: BAC25785.1
AK145537 mRNA Translation: BAE26492.1
AK169792 mRNA Translation: BAE41370.1
AC002397 Genomic DNA Translation: AAC36014.1
BC002006 mRNA Translation: AAH02006.1
BC027172 mRNA Translation: AAH27172.1 Different initiation.
CCDSiCCDS39629.1 [Q99M54-1]
RefSeqiNP_038566.1, NM_013538.5 [Q99M54-1]
XP_006505627.1, XM_006505564.3 [Q99M54-1]
XP_017176884.1, XM_017321395.1 [Q99M54-1]
UniGeneiMm.285723

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000024270

PTM databases

iPTMnetiQ99M54
PhosphoSitePlusiQ99M54

Proteomic databases

EPDiQ99M54
PaxDbiQ99M54
PeptideAtlasiQ99M54
PRIDEiQ99M54

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000024270; ENSMUSP00000024270; ENSMUSG00000023505 [Q99M54-1]
GeneIDi14793
KEGGimmu:14793
UCSCiuc009dsc.2 mouse [Q99M54-1]

Organism-specific databases

CTDi83461
MGIiMGI:1315198 Cdca3

Phylogenomic databases

eggNOGiENOG410IQ4X Eukaryota
ENOG410Y54T LUCA
GeneTreeiENSGT00390000017343
HOGENOMiHOG000013100
HOVERGENiHBG053225
InParanoidiQ99M54
OMAiGHDKENQ
OrthoDBiEOG091G0MF1
PhylomeDBiQ99M54
TreeFamiTF101068

Enzyme and pathway databases

UniPathwayi
UPA00143

Miscellaneous databases

PROiPR:Q99M54
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000023505 Expressed in 215 organ(s), highest expression level in testis
CleanExiMM_CDCA3
ExpressionAtlasiQ99M54 baseline and differential
GenevisibleiQ99M54 MM

Family and domain databases

InterProiView protein in InterPro
IPR038832 CDCA3
PANTHERiPTHR34756 PTHR34756, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCDCA3_MOUSE
AccessioniPrimary (citable) accession number: Q99M54
Secondary accession number(s): O88837, Q8R2V1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: May 15, 2007
Last modified: November 7, 2018
This is version 102 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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