UniProtKB - Q99M51 (NCK1_MOUSE)
Cytoplasmic protein NCK1
Nck1
Functioni
GO - Molecular functioni
- ephrin receptor binding Source: MGI
- eukaryotic initiation factor eIF2 binding Source: ParkinsonsUK-UCL
- protein domain specific binding Source: MGI
- protein kinase inhibitor activity Source: UniProtKB
- protein-macromolecule adaptor activity Source: MGI
- receptor tyrosine kinase binding Source: MGI
- signaling receptor binding Source: MGI
GO - Biological processi
- actin filament organization Source: MGI
- cell migration Source: MGI
- ephrin receptor signaling pathway Source: MGI
- lamellipodium assembly Source: MGI
- negative regulation of cell death Source: UniProtKB
- negative regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation Source: ParkinsonsUK-UCL
- negative regulation of insulin receptor signaling pathway Source: MGI
- negative regulation of peptidyl-serine phosphorylation Source: MGI
- negative regulation of PERK-mediated unfolded protein response Source: ParkinsonsUK-UCL
- negative regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress Source: ParkinsonsUK-UCL
- peptidyl-serine dephosphorylation Source: MGI
- positive regulation of actin filament polymerization Source: MGI
- positive regulation of cap-dependent translational initiation Source: MGI
- positive regulation of cap-independent translational initiation Source: MGI
- positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway Source: ParkinsonsUK-UCL
- positive regulation of neuron projection development Source: MGI
- positive regulation of T cell proliferation Source: MGI
- positive regulation of transcription by RNA polymerase II Source: UniProtKB
- positive regulation of translation in response to endoplasmic reticulum stress Source: ParkinsonsUK-UCL
- regulation of cell migration Source: MGI
- response to other organism Source: MGI
- substrate-dependent cell migration, cell extension Source: MGI
- T cell activation Source: MGI
Keywordsi
Biological process | Translation regulation |
Enzyme and pathway databases
Reactomei | R-MMU-186763, Downstream signal transduction R-MMU-202433, Generation of second messenger molecules R-MMU-373753, Nephrin family interactions R-MMU-418885, DCC mediated attractive signaling R-MMU-4420097, VEGFA-VEGFR2 Pathway R-MMU-983695, Antigen activates B Cell Receptor (BCR) leading to generation of second messengers |
Names & Taxonomyi
Protein namesi | Recommended name: Cytoplasmic protein NCK1Alternative name(s): NCK adaptor protein 1 Short name: Nck-1 |
Gene namesi | Name:Nck1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:109601, Nck1 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum By similarity
Nucleus
- Nucleus By similarity
Other locations
- Cytoplasm By similarity
Note: Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Import into the nucleus requires interaction with SOCS7. Predominantly nuclear following genotoxic stresses, such as UV irradiation, hydroxyurea or mitomycin C treatments (By similarity).By similarity
Cytosol
- cytosol Source: MGI
Endoplasmic reticulum
- endoplasmic reticulum Source: MGI
Nucleus
- nucleus Source: UniProtKB-SubCell
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- cell-cell junction Source: MGI
- cytoplasm Source: MGI
- protein phosphatase type 1 complex Source: MGI
- ribosome Source: MGI
- vesicle membrane Source: MGI
Keywords - Cellular componenti
Cytoplasm, Endoplasmic reticulum, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000413801 | 2 – 377 | Cytoplasmic protein NCK1Add BLAST | 376 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Modified residuei | 85 | PhosphoserineCombined sources | 1 | |
Modified residuei | 91 | PhosphoserineBy similarity | 1 | |
Modified residuei | 96 | PhosphoserineBy similarity | 1 | |
Modified residuei | 105 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 166 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q99M51 |
jPOSTi | Q99M51 |
MaxQBi | Q99M51 |
PaxDbi | Q99M51 |
PeptideAtlasi | Q99M51 |
PRIDEi | Q99M51 |
2D gel databases
REPRODUCTION-2DPAGEi | IPI00453999 |
PTM databases
iPTMneti | Q99M51 |
PhosphoSitePlusi | Q99M51 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000032475, Expressed in adult mammalian kidney and 283 other tissues |
ExpressionAtlasi | Q99M51, baseline and differential |
Genevisiblei | Q99M51, MM |
Interactioni
Subunit structurei
Associates with BLNK, PLCG1, VAV1 and NCK1 in a B-cell antigen receptor-dependent fashion.
Interacts with SOCS7. This interaction is required for nuclear import.
Part of a complex containing PPP1R15B, PP1 and NCK1.
Interacts with RALGPS1.
Interacts with CAV2 (tyrosine phosphorylated form).
Interacts with ADAM15.
Interacts with FASLG. Directly interacts with RASA1.
Interacts with MINK1.
Interacts with KDR (tyrosine phosphorylated).
Interacts (via SH2 domain) with EPHB1; activates the JUN cascade to regulate cell adhesion.
Interacts with EPHA2.
Interacts (via SH2 domain) with PDGFRB (tyrosine phosphorylated).
Interacts (via SH2 domain and SH3 domain 2) with EGFR.
Interacts with PAK1 and SOS1.
Interacts (via SH3 domains) with PKN2 (By similarity).
Interacts with FLT1 (tyrosine phosphorylated).
Interacts with the inactive form of EIF2AK2/PKR (By similarity).
By similarityBinary interactionsi
Hide detailsQ99M51
With | #Exp. | IntAct |
---|---|---|
Abl1 [P00520] | 2 | EBI-642202,EBI-914519 |
Dok1 [P97465] | 5 | EBI-642202,EBI-914917 |
Tnk2 [O54967] | 2 | EBI-642202,EBI-7780354 |
GO - Molecular functioni
- ephrin receptor binding Source: MGI
- eukaryotic initiation factor eIF2 binding Source: ParkinsonsUK-UCL
- protein domain specific binding Source: MGI
- receptor tyrosine kinase binding Source: MGI
- signaling receptor binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 201704, 49 interactors |
DIPi | DIP-37524N |
IntActi | Q99M51, 17 interactors |
MINTi | Q99M51 |
STRINGi | 10090.ENSMUSP00000112221 |
Miscellaneous databases
RNActi | Q99M51, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 61 | SH3 1PROSITE-ProRule annotationAdd BLAST | 60 | |
Domaini | 106 – 165 | SH3 2PROSITE-ProRule annotationAdd BLAST | 60 | |
Domaini | 190 – 252 | SH3 3PROSITE-ProRule annotationAdd BLAST | 63 | |
Domaini | 282 – 376 | SH2PROSITE-ProRule annotationAdd BLAST | 95 |
Domaini
Keywords - Domaini
Repeat, SH2 domain, SH3 domainPhylogenomic databases
eggNOGi | KOG4226, Eukaryota |
GeneTreei | ENSGT00940000156601 |
HOGENOMi | CLU_025160_0_1_1 |
InParanoidi | Q99M51 |
OMAi | KTGMRET |
OrthoDBi | 1097449at2759 |
PhylomeDBi | Q99M51 |
TreeFami | TF351631 |
Family and domain databases
CDDi | cd10408, SH2_Nck1, 1 hit cd11900, SH3_Nck1_1, 1 hit cd11901, SH3_Nck1_2, 1 hit cd11904, SH3_Nck1_3, 1 hit |
Gene3Di | 3.30.505.10, 1 hit |
InterProi | View protein in InterPro IPR017304, NCK IPR035882, Nck1_SH2 IPR035562, Nck1_SH3_1 IPR035564, Nck1_SH3_2 IPR035565, Nck1_SH3_3 IPR000980, SH2 IPR036860, SH2_dom_sf IPR036028, SH3-like_dom_sf IPR001452, SH3_domain |
Pfami | View protein in Pfam PF00017, SH2, 1 hit PF00018, SH3_1, 2 hits PF14604, SH3_9, 1 hit |
PIRSFi | PIRSF037874, Cytoplasmic_NCK, 1 hit |
PRINTSi | PR00401, SH2DOMAIN PR00452, SH3DOMAIN |
SMARTi | View protein in SMART SM00252, SH2, 1 hit SM00326, SH3, 3 hits |
SUPFAMi | SSF50044, SSF50044, 3 hits SSF55550, SSF55550, 1 hit |
PROSITEi | View protein in PROSITE PS50001, SH2, 1 hit PS50002, SH3, 3 hits |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MAEEVVVVAK FDYVAQQEQE LDIKKNERLW LLDDSKSWWR VRNSMNKTGF
60 70 80 90 100
VPSNYVERKN SARKASIVKN LKDTLGIGKV KRKPSVPDTA SPADDSFVDP
110 120 130 140 150
GERLYDLNMP AFVKFNYMAE REDELSLIKG TKVIVMEKCS DGWWRGSYNG
160 170 180 190 200
QIGWFPSNYV TEEGDSPLGD HVGSLSEKLA AVVNNLNTGQ VLHVVQALYP
210 220 230 240 250
FSSSNDEELN FEKGDVMDVI EKPENDPEWW KCRKINGMVG LVPKNYVTIM
260 270 280 290 300
QNNPLTSGLE PSPPQCDYIR PSLTGKFAGN PWYYGKVTRH QAEMALNERG
310 320 330 340 350
HEGDFLIRDS ESSPNDFSVS LKAQGKNKHF KVQLKETVYC IGQRKFSTME
360 370
ELVEHYKKAP IFTSEQGEKL YLVKHLS
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ8BH99 | Q8BH99_MOUSE | Cytoplasmic protein NCK1 | Nck1 | 313 | Annotation score: | ||
A0A087WSB1 | A0A087WSB1_MOUSE | Cytoplasmic protein NCK1 | Nck1 | 206 | Annotation score: | ||
A0A087WQD1 | A0A087WQD1_MOUSE | Cytoplasmic protein NCK1 | Nck1 | 132 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 4 | E → G in AAD13752 (Ref. 1) Curated | 1 | |
Sequence conflicti | 4 | E → G in AAC06352 (Ref. 2) Curated | 1 | |
Sequence conflicti | 30 | W → C in AAC06352 (Ref. 2) Curated | 1 | |
Sequence conflicti | 74 | T → N in AAC06352 (Ref. 2) Curated | 1 | |
Sequence conflicti | 84 | P → T in AAC06352 (Ref. 2) Curated | 1 | |
Sequence conflicti | 90 | A → E in AAC06352 (Ref. 2) Curated | 1 | |
Sequence conflicti | 155 – 157 | FPS → LPC in AAC06352 (Ref. 2) Curated | 3 | |
Sequence conflicti | 202 | S → G in AAC06352 (Ref. 2) Curated | 1 | |
Sequence conflicti | 206 | D → N in AAC06352 (Ref. 2) Curated | 1 | |
Sequence conflicti | 307 | I → V in AAC06352 (Ref. 2) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF084183 mRNA Translation: AAD13752.1 AF043259 mRNA Translation: AAC06352.1 AC129223 Genomic DNA No translation available. CH466560 Genomic DNA Translation: EDL21028.1 CH466560 Genomic DNA Translation: EDL21029.1 CH466560 Genomic DNA Translation: EDL21031.1 BC002015 mRNA Translation: AAH02015.1 |
CCDSi | CCDS23440.1 |
RefSeqi | NP_035008.2, NM_010878.3 XP_006510903.1, XM_006510840.2 |
Genome annotation databases
Ensembli | ENSMUST00000116522; ENSMUSP00000112221; ENSMUSG00000032475 |
GeneIDi | 17973 |
KEGGi | mmu:17973 |
UCSCi | uc009rex.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF084183 mRNA Translation: AAD13752.1 AF043259 mRNA Translation: AAC06352.1 AC129223 Genomic DNA No translation available. CH466560 Genomic DNA Translation: EDL21028.1 CH466560 Genomic DNA Translation: EDL21029.1 CH466560 Genomic DNA Translation: EDL21031.1 BC002015 mRNA Translation: AAH02015.1 |
CCDSi | CCDS23440.1 |
RefSeqi | NP_035008.2, NM_010878.3 XP_006510903.1, XM_006510840.2 |
3D structure databases
BMRBi | Q99M51 |
SMRi | Q99M51 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 201704, 49 interactors |
DIPi | DIP-37524N |
IntActi | Q99M51, 17 interactors |
MINTi | Q99M51 |
STRINGi | 10090.ENSMUSP00000112221 |
PTM databases
iPTMneti | Q99M51 |
PhosphoSitePlusi | Q99M51 |
2D gel databases
REPRODUCTION-2DPAGEi | IPI00453999 |
Proteomic databases
EPDi | Q99M51 |
jPOSTi | Q99M51 |
MaxQBi | Q99M51 |
PaxDbi | Q99M51 |
PeptideAtlasi | Q99M51 |
PRIDEi | Q99M51 |
Protocols and materials databases
Antibodypediai | 3567, 538 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000116522; ENSMUSP00000112221; ENSMUSG00000032475 |
GeneIDi | 17973 |
KEGGi | mmu:17973 |
UCSCi | uc009rex.1, mouse |
Organism-specific databases
CTDi | 4690 |
MGIi | MGI:109601, Nck1 |
Phylogenomic databases
eggNOGi | KOG4226, Eukaryota |
GeneTreei | ENSGT00940000156601 |
HOGENOMi | CLU_025160_0_1_1 |
InParanoidi | Q99M51 |
OMAi | KTGMRET |
OrthoDBi | 1097449at2759 |
PhylomeDBi | Q99M51 |
TreeFami | TF351631 |
Enzyme and pathway databases
Reactomei | R-MMU-186763, Downstream signal transduction R-MMU-202433, Generation of second messenger molecules R-MMU-373753, Nephrin family interactions R-MMU-418885, DCC mediated attractive signaling R-MMU-4420097, VEGFA-VEGFR2 Pathway R-MMU-983695, Antigen activates B Cell Receptor (BCR) leading to generation of second messengers |
Miscellaneous databases
BioGRID-ORCSi | 17973, 0 hits in 17 CRISPR screens |
ChiTaRSi | Nck1, mouse |
PROi | PR:Q99M51 |
RNActi | Q99M51, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000032475, Expressed in adult mammalian kidney and 283 other tissues |
ExpressionAtlasi | Q99M51, baseline and differential |
Genevisiblei | Q99M51, MM |
Family and domain databases
CDDi | cd10408, SH2_Nck1, 1 hit cd11900, SH3_Nck1_1, 1 hit cd11901, SH3_Nck1_2, 1 hit cd11904, SH3_Nck1_3, 1 hit |
Gene3Di | 3.30.505.10, 1 hit |
InterProi | View protein in InterPro IPR017304, NCK IPR035882, Nck1_SH2 IPR035562, Nck1_SH3_1 IPR035564, Nck1_SH3_2 IPR035565, Nck1_SH3_3 IPR000980, SH2 IPR036860, SH2_dom_sf IPR036028, SH3-like_dom_sf IPR001452, SH3_domain |
Pfami | View protein in Pfam PF00017, SH2, 1 hit PF00018, SH3_1, 2 hits PF14604, SH3_9, 1 hit |
PIRSFi | PIRSF037874, Cytoplasmic_NCK, 1 hit |
PRINTSi | PR00401, SH2DOMAIN PR00452, SH3DOMAIN |
SMARTi | View protein in SMART SM00252, SH2, 1 hit SM00326, SH3, 3 hits |
SUPFAMi | SSF50044, SSF50044, 3 hits SSF55550, SSF55550, 1 hit |
PROSITEi | View protein in PROSITE PS50001, SH2, 1 hit PS50002, SH3, 3 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NCK1_MOUSE | |
Accessioni | Q99M51Primary (citable) accession number: Q99M51 Secondary accession number(s): O55032, Q9Z279 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 16, 2011 |
Last sequence update: | June 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 161 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot