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Protein

Lipoyl synthase, mitochondrial

Gene

Lias

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.Curated

Catalytic activityi

Protein N6-(octanoyl)lysine + an [Fe-S] cluster scaffold protein carrying a [4Fe-4S]2+ cluster + 2 S-adenosyl-L-methionine + 2 oxidized [2Fe-2S] ferredoxin + 6 H+ = protein N6-(dihydrolipoyl)lysine + an [Fe-S] cluster scaffold protein + 2 sulfide + 4 Fe3+ + 2 L-methionine + 2 5'-deoxyadenosine + 2 reduced [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Putative lipoyltransferase 2, mitochondrial (Lipt2)
  2. Lipoyl synthase, mitochondrial (Lias), Lipoyl synthase, mitochondrial (Lias), Lipoyl synthase, mitochondrial (Lias)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi105Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi110Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi116Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi136Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi140Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi143Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

  • 4 iron, 4 sulfur cluster binding Source: UniProtKB-UniRule
  • lipoate synthase activity Source: MGI
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionTransferase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-MMU-389661 Glyoxylate metabolism and glycine degradation
UniPathwayi
UPA00538;UER00593

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lipoate synthaseUniRule annotation
Short name:
LSUniRule annotation
Short name:
Lip-synUniRule annotation
Short name:
mLIP1
Lipoic acid synthaseUniRule annotation
Gene namesi
Name:Lias
Synonyms:Lip1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1934604 Lias

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 26MitochondrionUniRule annotationAdd BLAST26
ChainiPRO_000001772427 – 373Lipoyl synthase, mitochondrialAdd BLAST347

Proteomic databases

EPDiQ99M04
MaxQBiQ99M04
PaxDbiQ99M04
PRIDEiQ99M04

PTM databases

PhosphoSitePlusiQ99M04

Expressioni

Tissue specificityi

Expressed predominantly in heart, testis, and liver.1 Publication

Gene expression databases

BgeeiENSMUSG00000029199 Expressed in 286 organ(s), highest expression level in ear
CleanExiMM_LIAS
ExpressionAtlasiQ99M04 baseline and differential
GenevisibleiQ99M04 MM

Interactioni

Protein-protein interaction databases

BioGridi219762, 1 interactor
STRINGi10090.ENSMUSP00000031101

Structurei

3D structure databases

ProteinModelPortaliQ99M04
SMRiQ99M04
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2672 Eukaryota
COG0320 LUCA
GeneTreeiENSGT00390000006234
HOGENOMiHOG000235998
HOVERGENiHBG023328
InParanoidiQ99M04
KOiK03644
OMAiPYCDIDF
OrthoDBiEOG091G0AXJ
PhylomeDBiQ99M04
TreeFamiTF300817

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00206 Lipoyl_synth, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006638 Elp3/MiaB/NifB
IPR031691 LIAS_N
IPR003698 Lipoyl_synth
IPR007197 rSAM
PANTHERiPTHR10949 PTHR10949, 1 hit
PfamiView protein in Pfam
PF16881 LIAS_N, 1 hit
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF005963 Lipoyl_synth, 1 hit
SFLDiSFLDF00271 lipoyl_synthase, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00510 lipA, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

Q99M04-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MALRCWDTAR SLGSRIFGRY AFTVRALSSL PDKKKEFLHN GPDLQDFVSG
60 70 80 90 100
DLADKSTWDE YKGNLKRQKG ERLRLPPWLK TKIPMGKNYN KLKNTLRNLS
110 120 130 140 150
LHTVCEEARC PNIGECWGGG EYATATATIM LMGDTCTRGC RFCSVKTARN
160 170 180 190 200
PPPLDANEPD NTAKAIAEWG LDYVVLTSVD RDDVADGGAE HIAKTVSCLK
210 220 230 240 250
ERNPKILVEC LTPDFRGDLR AVEKVALSGL DVYAHNVETV PELQRKVRDP
260 270 280 290 300
RANFDQSLRV LRHAKEVQPD VVSKTSIMLG LGETDEQVYA TLKALRAADV
310 320 330 340 350
DCLTLGQYMQ PTKRHLKVEE YVTPEKFKYW EKVGNELGFL YTASGPLVRS
360 370
SYKAGEFFLK NLVARRKTKA SKV
Length:373
Mass (Da):41,879
Last modified:June 1, 2001 - v1
Checksum:i6796340A54BC5B8E
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0M3HEP3A0A0M3HEP3_MOUSE
Lipoyl synthase, mitochondrial
Lias LIAS
289Annotation score:
A0A0G2JFF7A0A0G2JFF7_MOUSE
Lipoyl synthase, mitochondrial
Lias
74Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB057731 mRNA Translation: BAB62009.1
AK048744 mRNA Translation: BAC33443.1
AK049548 mRNA Translation: BAC33804.1
AK077192 mRNA Translation: BAC36672.1
BC002141 mRNA Translation: AAH02141.1
CCDSiCCDS19307.1
RefSeqiNP_001297541.1, NM_001310612.1
NP_077791.1, NM_024471.5
UniGeneiMm.195776

Genome annotation databases

EnsembliENSMUST00000122026; ENSMUSP00000113228; ENSMUSG00000029199
GeneIDi79464
KEGGimmu:79464
UCSCiuc008xnq.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB057731 mRNA Translation: BAB62009.1
AK048744 mRNA Translation: BAC33443.1
AK049548 mRNA Translation: BAC33804.1
AK077192 mRNA Translation: BAC36672.1
BC002141 mRNA Translation: AAH02141.1
CCDSiCCDS19307.1
RefSeqiNP_001297541.1, NM_001310612.1
NP_077791.1, NM_024471.5
UniGeneiMm.195776

3D structure databases

ProteinModelPortaliQ99M04
SMRiQ99M04
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi219762, 1 interactor
STRINGi10090.ENSMUSP00000031101

PTM databases

PhosphoSitePlusiQ99M04

Proteomic databases

EPDiQ99M04
MaxQBiQ99M04
PaxDbiQ99M04
PRIDEiQ99M04

Protocols and materials databases

DNASUi79464
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000122026; ENSMUSP00000113228; ENSMUSG00000029199
GeneIDi79464
KEGGimmu:79464
UCSCiuc008xnq.1 mouse

Organism-specific databases

CTDi11019
MGIiMGI:1934604 Lias

Phylogenomic databases

eggNOGiKOG2672 Eukaryota
COG0320 LUCA
GeneTreeiENSGT00390000006234
HOGENOMiHOG000235998
HOVERGENiHBG023328
InParanoidiQ99M04
KOiK03644
OMAiPYCDIDF
OrthoDBiEOG091G0AXJ
PhylomeDBiQ99M04
TreeFamiTF300817

Enzyme and pathway databases

UniPathwayi
UPA00538;UER00593

ReactomeiR-MMU-389661 Glyoxylate metabolism and glycine degradation

Miscellaneous databases

ChiTaRSiLipa mouse
PROiPR:Q99M04
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029199 Expressed in 286 organ(s), highest expression level in ear
CleanExiMM_LIAS
ExpressionAtlasiQ99M04 baseline and differential
GenevisibleiQ99M04 MM

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00206 Lipoyl_synth, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006638 Elp3/MiaB/NifB
IPR031691 LIAS_N
IPR003698 Lipoyl_synth
IPR007197 rSAM
PANTHERiPTHR10949 PTHR10949, 1 hit
PfamiView protein in Pfam
PF16881 LIAS_N, 1 hit
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF005963 Lipoyl_synth, 1 hit
SFLDiSFLDF00271 lipoyl_synthase, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00510 lipA, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiLIAS_MOUSE
AccessioniPrimary (citable) accession number: Q99M04
Secondary accession number(s): Q543M1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: June 1, 2001
Last modified: October 10, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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