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UniProtKB - Q99LM3 (SMTL1_MOUSE)
Protein
Smoothelin-like protein 1
Gene
Smtnl1
Organism
Mus musculus (Mouse)
Status
Functioni
Plays a role in the regulation of contractile properties of both striated and smooth muscles. When unphosphorylated, may inhibit myosin dephosphorylation. Phosphorylation at Ser-301 reduces this inhibitory activity.
2 PublicationsGO - Molecular functioni
- calmodulin binding Source: CAFA
- CH domain binding Source: CAFA
- disordered domain specific binding Source: CAFA
- myosin phosphatase regulator activity Source: MGI
- protein phosphatase 1 binding Source: UniProtKB
- protein phosphatase inhibitor activity Source: MGI
- protein self-association Source: CAFA
- tropomyosin binding Source: CAFA
GO - Biological processi
- actin cytoskeleton organization Source: GO_Central
- muscle organ morphogenesis Source: MGI
- negative regulation of transcription, DNA-templated Source: CACAO
- peptidyl-serine phosphorylation Source: MGI
- positive regulation of vasoconstriction Source: UniProtKB
- response to activity Source: MGI
- response to xenobiotic stimulus Source: MGI
- vasoconstriction Source: MGI
Keywordsi
Molecular function | Calmodulin-binding, Muscle protein |
Names & Taxonomyi
Protein namesi | Recommended name: Smoothelin-like protein 1Alternative name(s): Calponin homology-associated smooth muscle protein Short name: CHASM |
Gene namesi | Name:Smtnl1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1915928, Smtnl1 |
VEuPathDBi | HostDB:ENSMUSG00000027077 |
Subcellular locationi
Cytoskeleton
- filamentous actin Source: GO_Central
- microtubule organizing center Source: GO_Central
Nucleus
- nucleus Source: UniProtKB
Other locations
- contractile fiber Source: UniProtKB
- cytoplasm Source: UniProtKB
- I band Source: MGI
- M band Source: MGI
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Disruption phenotypei
Male mutant mice perform better than wild type in exercise stress test after endurance training. Females do not differ significantly during these tests. Even in the absence of endurance exercise, mutant mice exhibit muscle fiber adaptation, i.e. more type 2a fibers and lower levels of type 1b fibers. Endothelium-dependent vasorelaxation of the aorta is enhanced and responses to beta-adrenergic constriction are reduced. Expression of PPP1R12A is 30-40-fold higher in mutant mice than in wild-type littermates and exhibits a steady decline as the animals become sexually mature (at protein level). During pregnancy, by day 13, PPP1R12A expression is dramatically increased to 6-14 times over the levels observed in pregnant wild-type littermates (at protein level). PPP1R12B expression levels are unaffected. In vascular smooth muscle, force development in response to phenylephrine is reduced and both the rate and extent of relaxation in response to acetylcholine are promoted. Myosin dephosphorylation is promoted in mutant animals.2 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 301 | S → A: Loss of phosphorylation. Loss of nuclear localization. 3 Publications | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000317276 | 1 – 459 | Smoothelin-like protein 1Add BLAST | 459 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 301 | Phosphoserine; by PKA and PKG3 Publications | 1 |
Post-translational modificationi
Maximal phosphorylation of Ser-301 correlates with maximal relaxation of aorta in response to acetylcholine.3 Publications
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q99LM3 |
PeptideAtlasi | Q99LM3 |
PRIDEi | Q99LM3 |
ProteomicsDBi | 261282 |
PTM databases
iPTMneti | Q99LM3 |
PhosphoSitePlusi | Q99LM3 |
Expressioni
Tissue specificityi
Widely expressed, with highest expression in skeletal muscles (at protein level). Within striated muscles, significantly more expressed in soleus muscle compared with plantaris muscle or white vastus (at protein level). 30-40% lower expression in females than in males (at protein level). Expressed in type 2a fibers, but not detected in fast twitch type 2b muscle white vastus nor in oxidative type I/b heart muscle (at protein level). Expressed within myometrial cells of the uterus, as well as in the endometrial layer. In the aorta, confined to smooth muscle cells. Not detected in endothelial cells.2 Publications
Developmental stagei
Not detected in somites which give rise to skeletal muscle at 10.5 dpc (at protein level). Expressed in skeletal muscle of the tongue, diaphragm and axial muscles from 14.5 through 17.5 dpc (at protein level). Not detected in limb buds (at protein level). Overall increase by up to 10-12-fold in vascular and uterine smooth muscle during pregnancy (at protein level). At day 13 of pregnancy, expression increases in striated muscle by 2.5-fold compared with non-pregnant mice, and by about 2-fold over levels expressed in males (at protein level). At the same time, dramatically increased in myometrial cells of the uterus, in the endometrial layer and in aortal smooth muscle. Steadily declines through parturition and the onset of lactation (at protein level).2 Publications
Inductioni
Significantly reduced by exercise in smooth and in skeletal muscles.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000027077, Expressed in tarsal region and 79 other tissues |
Genevisiblei | Q99LM3, MM |
Interactioni
Subunit structurei
Interacts with PPP1R12A.
1 PublicationBinary interactionsi
Q99LM3
With | #Exp. | IntAct |
---|---|---|
TPM1 [P04268] from Gallus gallus. | 3 | EBI-8073484,EBI-8073544 |
GO - Molecular functioni
- calmodulin binding Source: CAFA
- CH domain binding Source: CAFA
- disordered domain specific binding Source: CAFA
- protein phosphatase 1 binding Source: UniProtKB
- protein self-association Source: CAFA
- tropomyosin binding Source: CAFA
Protein-protein interaction databases
IntActi | Q99LM3, 4 interactors |
MINTi | Q99LM3 |
STRINGi | 10090.ENSMUSP00000028471 |
Miscellaneous databases
RNActi | Q99LM3, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q99LM3 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q99LM3 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 343 – 449 | Calponin-homology (CH)PROSITE-ProRule annotationAdd BLAST | 107 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 314 | DisorderedSequence analysisAdd BLAST | 314 | |
Regioni | 441 – 459 | Calmodulin-bindingAdd BLAST | 19 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 124 – 154 | Sequence analysisAdd BLAST | 31 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 25 | Polar residuesSequence analysisAdd BLAST | 25 | |
Compositional biasi | 77 – 172 | Basic and acidic residuesSequence analysisAdd BLAST | 96 | |
Compositional biasi | 184 – 231 | Basic and acidic residuesSequence analysisAdd BLAST | 48 | |
Compositional biasi | 259 – 279 | Polar residuesSequence analysisAdd BLAST | 21 | |
Compositional biasi | 286 – 304 | Basic and acidic residuesSequence analysisAdd BLAST | 19 |
Sequence similaritiesi
Belongs to the smoothelin family.Curated
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG4678, Eukaryota |
GeneTreei | ENSGT00940000162276 |
HOGENOMi | CLU_040651_5_1_1 |
InParanoidi | Q99LM3 |
OMAi | TVASQEM |
OrthoDBi | 168604at2759 |
PhylomeDBi | Q99LM3 |
TreeFami | TF316716 |
Family and domain databases
CDDi | cd00014, CH, 1 hit |
DisProti | DP00742 |
Gene3Di | 1.10.418.10, 1 hit |
IDEALi | IID50181 |
InterProi | View protein in InterPro IPR001715, CH-domain IPR036872, CH_dom_sf |
Pfami | View protein in Pfam PF00307, CH, 1 hit |
SMARTi | View protein in SMART SM00033, CH, 1 hit |
SUPFAMi | SSF47576, SSF47576, 1 hit |
PROSITEi | View protein in PROSITE PS50021, CH, 1 hit |
i Sequence
Sequence statusi: Complete.
Q99LM3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEQTEGNSSE DGTTVSPTAG NLETPGSQGI AEEVAEGTVG TSDKEGPSDW
60 70 80 90 100
AEHLCKAASK SGESGGSPGE ASILDELKTD LQGEARGKDE AQGDLAEEKV
110 120 130 140 150
GKEDTTAASQ EDTGKKEETK PEPNEVREKE EAMLASEKQK VDEKETNLES
160 170 180 190 200
KEKSDVNDKA KPEPKEDAGA EVTVNEAETE SQEEADVKDQ AKPELPEVDG
210 220 230 240 250
KETGSDTKEL VEPESPTEEQ EQGKENESEE RAAVIPSSPE EWPESPTDEG
260 270 280 290 300
PSLSPDGLAP ESTGETSPSA SESSPSEVPG SPTEPQPSEK KKDRAPERRV
310 320 330 340 350
SAPSRPRGPR AQNRKAIMDK FGGAASGPTA LFRNTKAAGA AIGGVKNMLL
360 370 380 390 400
EWCRAMTRNY EHVDIQNFSS SWSSGMAFCA LIHKFFPEAF DYAELDPAKR
410 420 430 440 450
RHNFTLAFST AEKLADCAQL LEVDDMVRLA VPDSKCVYTY IQELYRSLVQ
KGLVKTKKK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL928914 Genomic DNA No translation available. BC002317 mRNA Translation: AAH02317.1 |
CCDSi | CCDS16195.1 |
RefSeqi | NP_077192.1, NM_024230.2 XP_006500172.1, XM_006500109.3 |
Genome annotation databases
Ensembli | ENSMUST00000028471; ENSMUSP00000028471; ENSMUSG00000027077 |
GeneIDi | 68678 |
KEGGi | mmu:68678 |
UCSCi | uc008kjg.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL928914 Genomic DNA No translation available. BC002317 mRNA Translation: AAH02317.1 |
CCDSi | CCDS16195.1 |
RefSeqi | NP_077192.1, NM_024230.2 XP_006500172.1, XM_006500109.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2JV9 | NMR | - | A | 346-459 | [»] | |
2K3S | NMR | - | A | 346-459 | [»] | |
SMRi | Q99LM3 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | Q99LM3, 4 interactors |
MINTi | Q99LM3 |
STRINGi | 10090.ENSMUSP00000028471 |
PTM databases
iPTMneti | Q99LM3 |
PhosphoSitePlusi | Q99LM3 |
Proteomic databases
PaxDbi | Q99LM3 |
PeptideAtlasi | Q99LM3 |
PRIDEi | Q99LM3 |
ProteomicsDBi | 261282 |
Protocols and materials databases
Antibodypediai | 48478, 73 antibodies from 15 providers |
DNASUi | 68678 |
Genome annotation databases
Ensembli | ENSMUST00000028471; ENSMUSP00000028471; ENSMUSG00000027077 |
GeneIDi | 68678 |
KEGGi | mmu:68678 |
UCSCi | uc008kjg.1, mouse |
Organism-specific databases
CTDi | 219537 |
MGIi | MGI:1915928, Smtnl1 |
VEuPathDBi | HostDB:ENSMUSG00000027077 |
Phylogenomic databases
eggNOGi | KOG4678, Eukaryota |
GeneTreei | ENSGT00940000162276 |
HOGENOMi | CLU_040651_5_1_1 |
InParanoidi | Q99LM3 |
OMAi | TVASQEM |
OrthoDBi | 168604at2759 |
PhylomeDBi | Q99LM3 |
TreeFami | TF316716 |
Miscellaneous databases
BioGRID-ORCSi | 68678, 2 hits in 64 CRISPR screens |
EvolutionaryTracei | Q99LM3 |
PROi | PR:Q99LM3 |
RNActi | Q99LM3, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000027077, Expressed in tarsal region and 79 other tissues |
Genevisiblei | Q99LM3, MM |
Family and domain databases
CDDi | cd00014, CH, 1 hit |
DisProti | DP00742 |
Gene3Di | 1.10.418.10, 1 hit |
IDEALi | IID50181 |
InterProi | View protein in InterPro IPR001715, CH-domain IPR036872, CH_dom_sf |
Pfami | View protein in Pfam PF00307, CH, 1 hit |
SMARTi | View protein in SMART SM00033, CH, 1 hit |
SUPFAMi | SSF47576, SSF47576, 1 hit |
PROSITEi | View protein in PROSITE PS50021, CH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SMTL1_MOUSE | |
Accessioni | Q99LM3Primary (citable) accession number: Q99LM3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 5, 2008 |
Last sequence update: | June 1, 2001 | |
Last modified: | February 23, 2022 | |
This is version 139 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families