Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

CDK5 regulatory subunit-associated protein 3

Gene

Cdk5rap3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable tumor suppressor initially identified as a CDK5R1 interactor controlling cell proliferation. Negatively regulates NF-kappa-B-mediated gene transcription through the control of RELA phosphorylation. Also regulates mitotic G2/M transition checkpoint and mitotic G2 DNA damage checkpoint. Through its interaction with CDKN2A/ARF and MDM2 may induce MDM2-dependent p53/TP53 ubiquitination, stabilization and activation in the nucleus, thereby promoting G1 cell cycle arrest and inhibition of cell proliferation. May play a role in the unfolded protein response, mediating the ufmylation of multiple proteins in response to endoplasmic reticulum stress. May also play a role in the rupture of the nuclear envelope during apoptosis. May regulate MAPK14 activity by regulating its dephosphorylation by PPM1D/WIP1.By similarity

GO - Molecular functioni

GO - Biological processi

Names & Taxonomyi

Protein namesi
Recommended name:
CDK5 regulatory subunit-associated protein 3Curated
Gene namesi
Name:Cdk5rap3Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1933126 Cdk5rap3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002205171 – 503CDK5 regulatory subunit-associated protein 3Add BLAST503

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki447Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Post-translational modificationi

May be phosphorylated by CDK5.By similarity
May be ufmylated.1 Publication
Ubiquitinated. Probably triggers proteasomal degradation and is negatively regulated by UFL1.By similarity
Cleaved by caspases early during apoptosis, the resulting peptides may play a role in rupture of the nuclear envelope.By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ99LM2
MaxQBiQ99LM2
PaxDbiQ99LM2
PRIDEiQ99LM2

PTM databases

iPTMnetiQ99LM2
PhosphoSitePlusiQ99LM2

Expressioni

Tissue specificityi

Widely expressed with higher expression in secretory tissues.1 Publication

Gene expression databases

BgeeiENSMUSG00000018669 Expressed in 291 organ(s), highest expression level in lacrimal gland
CleanExiMM_CDK5RAP3
ExpressionAtlasiQ99LM2 baseline and differential
GenevisibleiQ99LM2 MM

Interactioni

Subunit structurei

Interacts with CDK5R1; competes with CDK5RAP1 and CDK5RAP2. Interacts with RELA. Interacts with CHEK1; may negatively regulate CHEK1 and thereby stimulate entry into mitosis. Interacts with CDKN2A/ARF and MDM2; forms a ternary complex involved in regulation of p53/TP53 (By similarity). Interacts with UFL1; the interaction is direct (PubMed:21494687). Interacts with DDRGK1. Interacts with MAPK14. Interacts with CCNB1. Interacts with TUBG1; may regulate CDK5RAP3 in mitotic G2/M transition checkpoint (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ99LM2, 1 interactor
STRINGi10090.ENSMUSP00000099441

Structurei

3D structure databases

ProteinModelPortaliQ99LM2
SMRiQ99LM2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni268 – 503Required for interaction with UFL1 and mediates interaction with CHEK1By similarityAdd BLAST236

Sequence similaritiesi

Belongs to the CDK5RAP3 family.Curated

Phylogenomic databases

eggNOGiKOG2607 Eukaryota
ENOG410XSWB LUCA
GeneTreeiENSGT00390000000713
HOGENOMiHOG000231047
HOVERGENiHBG050978
InParanoidiQ99LM2
OMAiPGVRKQM
OrthoDBiEOG091G11PB
PhylomeDBiQ99LM2

Family and domain databases

InterProiView protein in InterPro
IPR008491 CDK5RAP3
PANTHERiPTHR14894 PTHR14894, 1 hit
PfamiView protein in Pfam
PF05600 DUF773, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 7 potential isoforms that are computationally mapped.Show allAlign All

Q99LM2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MQDHQHVPID IQTSKLLDWL VDRRHCNLKW QSLVLTIREK INTAIQDMPE
60 70 80 90 100
SQEIAQLLSG SYIHYFHCLR IVDLLKGTEA STKNIFGRYS SQRMKDWQEI
110 120 130 140 150
VSLYEKDNTY LVELCSLLVR NVSYEIPSLK KQIAKCQQLQ QEYSRKEEEG
160 170 180 190 200
QAGAAEMREQ FYHSCKQYGI TGDNVRRELL ALVKDLPSQL AEIGAGAQSL
210 220 230 240 250
GEAIDLYQAC VEFVCDSPTE QVLPMLRYVQ KKGNSTVYEW RTGTEPSVVE
260 270 280 290 300
RPQLEEPPEQ VQEDEIDWGD FGVEAVSDSG IVAETPGIDW GISLESEAKD
310 320 330 340 350
AGADKIDWGD DAAAASEITV LETGTEAPEG VARGSDALTL LEYPETRNQF
360 370 380 390 400
IDELMELEIF LSQRAVEMSE EADILSVSQF QLAPAILQGQ TKEKMLSLVS
410 420 430 440 450
TLQQLIGRLT SLRMQHLFMI LASPRYVDRV TEFLQQKLKQ SQLLALKKEL
460 470 480 490 500
MVEKQQEALQ EQAALEPKLD LLLEKTRELQ KLIEADISKR YSGRPVNLMG

TSL
Length:503
Mass (Da):56,991
Last modified:June 1, 2001 - v1
Checksum:i61428829BAB406E3
GO

Computationally mapped potential isoform sequencesi

There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A2A6E1A2A6E1_MOUSE
CDK5 regulatory subunit-associated ...
Cdk5rap3
124Annotation score:
A2A6D9A2A6D9_MOUSE
CDK5 regulatory subunit-associated ...
Cdk5rap3
140Annotation score:
B0QZI8B0QZI8_MOUSE
CDK5 regulatory subunit-associated ...
Cdk5rap3
145Annotation score:
A2A6E0A2A6E0_MOUSE
CDK5 regulatory subunit-associated ...
Cdk5rap3
80Annotation score:
D6RJ46D6RJ46_MOUSE
CDK5 regulatory subunit-associated ...
Cdk5rap3
112Annotation score:
F2Z486F2Z486_MOUSE
CDK5 regulatory subunit-associated ...
Cdk5rap3
142Annotation score:
D6RII4D6RII4_MOUSE
CDK5 regulatory subunit-associated ...
Cdk5rap3
45Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK075771 mRNA Translation: BAC35945.1
AK075791 mRNA Translation: BAC35961.1
AK077853 mRNA Translation: BAC37034.1
AK149765 mRNA Translation: BAE29070.1
BC002318 mRNA Translation: AAH02318.1
CCDSiCCDS25306.1
RefSeqiNP_084524.1, NM_030248.2
UniGeneiMm.28297
Mm.404690

Genome annotation databases

EnsembliENSMUST00000103152; ENSMUSP00000099441; ENSMUSG00000018669
GeneIDi80280
KEGGimmu:80280
UCSCiuc007lcv.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK075771 mRNA Translation: BAC35945.1
AK075791 mRNA Translation: BAC35961.1
AK077853 mRNA Translation: BAC37034.1
AK149765 mRNA Translation: BAE29070.1
BC002318 mRNA Translation: AAH02318.1
CCDSiCCDS25306.1
RefSeqiNP_084524.1, NM_030248.2
UniGeneiMm.28297
Mm.404690

3D structure databases

ProteinModelPortaliQ99LM2
SMRiQ99LM2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ99LM2, 1 interactor
STRINGi10090.ENSMUSP00000099441

PTM databases

iPTMnetiQ99LM2
PhosphoSitePlusiQ99LM2

Proteomic databases

EPDiQ99LM2
MaxQBiQ99LM2
PaxDbiQ99LM2
PRIDEiQ99LM2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000103152; ENSMUSP00000099441; ENSMUSG00000018669
GeneIDi80280
KEGGimmu:80280
UCSCiuc007lcv.1 mouse

Organism-specific databases

CTDi80279
MGIiMGI:1933126 Cdk5rap3

Phylogenomic databases

eggNOGiKOG2607 Eukaryota
ENOG410XSWB LUCA
GeneTreeiENSGT00390000000713
HOGENOMiHOG000231047
HOVERGENiHBG050978
InParanoidiQ99LM2
OMAiPGVRKQM
OrthoDBiEOG091G11PB
PhylomeDBiQ99LM2

Miscellaneous databases

PROiPR:Q99LM2
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000018669 Expressed in 291 organ(s), highest expression level in lacrimal gland
CleanExiMM_CDK5RAP3
ExpressionAtlasiQ99LM2 baseline and differential
GenevisibleiQ99LM2 MM

Family and domain databases

InterProiView protein in InterPro
IPR008491 CDK5RAP3
PANTHERiPTHR14894 PTHR14894, 1 hit
PfamiView protein in Pfam
PF05600 DUF773, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCK5P3_MOUSE
AccessioniPrimary (citable) accession number: Q99LM2
Secondary accession number(s): Q3UE41
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: June 1, 2001
Last modified: November 7, 2018
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again