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Protein

N(G),N(G)-dimethylarginine dimethylaminohydrolase 2

Gene

Ddah2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation (By similarity).By similarity

Catalytic activityi

N(omega),N(omega)-dimethyl-L-arginine + H2O = dimethylamine + L-citrulline.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei171Proton donorBy similarity1
Active sitei276NucleophileSequence analysis1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

BRENDAi3.5.3.18 3474
ReactomeiR-MMU-203615 eNOS activation

Names & Taxonomyi

Protein namesi
Recommended name:
N(G),N(G)-dimethylarginine dimethylaminohydrolase 2 (EC:3.5.3.18)
Short name:
DDAH-2
Short name:
Dimethylarginine dimethylaminohydrolase 2
Alternative name(s):
DDAHII
Dimethylargininase-2
Gene namesi
Name:Ddah2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1859016 Ddah2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001711221 – 285N(G),N(G)-dimethylarginine dimethylaminohydrolase 2Add BLAST285

Proteomic databases

EPDiQ99LD8
MaxQBiQ99LD8
PaxDbiQ99LD8
PeptideAtlasiQ99LD8
PRIDEiQ99LD8

2D gel databases

REPRODUCTION-2DPAGEiIPI00336881
UCD-2DPAGEiQ99LD8

PTM databases

iPTMnetiQ99LD8
PhosphoSitePlusiQ99LD8

Expressioni

Gene expression databases

BgeeiENSMUSG00000007039 Expressed in 254 organ(s), highest expression level in cerebral cortex
CleanExiMM_DDAH2
ExpressionAtlasiQ99LD8 baseline and differential
GenevisibleiQ99LD8 MM

Interactioni

Protein-protein interaction databases

BioGridi206177, 1 interactor
STRINGi10090.ENSMUSP00000007255

Structurei

3D structure databases

ProteinModelPortaliQ99LD8
SMRiQ99LD8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DDAH family.Curated

Phylogenomic databases

eggNOGiENOG410ISNS Eukaryota
ENOG4111YMV LUCA
GeneTreeiENSGT00390000009331
HOGENOMiHOG000161035
HOVERGENiHBG055937
InParanoidiQ99LD8
KOiK01482
OMAiAQRQFGC
OrthoDBiEOG091G0M5S
PhylomeDBiQ99LD8
TreeFamiTF314737

Family and domain databases

InterProiView protein in InterPro
IPR033199 DDAH/AD
IPR033202 DDAH2
PANTHERiPTHR12737 PTHR12737, 1 hit
PTHR12737:SF16 PTHR12737:SF16, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

Q99LD8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGTPGEGLGR CSHALIRGVP ESLASGEGAG AGLPALDLAK AQREHGVLGG
60 70 80 90 100
KLRQRLGLQL LELPPEESLP LGPLLGDTAV IQGDTALITR PWSPARRPEV
110 120 130 140 150
DGVRKALQDL GLRIVEMGDE NATLDGTDVL FTGREFFVGL SKWTNHRGAE
160 170 180 190 200
IVADTFRDFA VSTVPVSGSS HLRGLCGMGG PRTVVAGSSE AAQKAVRAMA
210 220 230 240 250
ALTDHPYASL TLPDDAASDC LFLRPGLPGA TPFLLHRGGG DLPNSQEALQ
260 270 280
KLSDVTLVPV SCSELEKAGA GLSSLCLVLS TRPHC
Length:285
Mass (Da):29,646
Last modified:June 1, 2001 - v1
Checksum:i3795D0BA2D314EE5
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
O08972O08972_MOUSE
Dimethylarginine dimethylaminohydro...
Ddah2 mCG_15913
244Annotation score:
G3UZR0G3UZR0_MOUSE
N(G),N(G)-dimethylarginine dimethyl...
Ddah2
157Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC003328 mRNA Translation: AAH03328.1
CCDSiCCDS50082.1
RefSeqiNP_001177378.1, NM_001190449.1
XP_006524675.1, XM_006524612.3
UniGeneiMm.1457

Genome annotation databases

EnsembliENSMUST00000007255; ENSMUSP00000007255; ENSMUSG00000007039
GeneIDi51793
KEGGimmu:51793
UCSCiuc008cfi.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC003328 mRNA Translation: AAH03328.1
CCDSiCCDS50082.1
RefSeqiNP_001177378.1, NM_001190449.1
XP_006524675.1, XM_006524612.3
UniGeneiMm.1457

3D structure databases

ProteinModelPortaliQ99LD8
SMRiQ99LD8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi206177, 1 interactor
STRINGi10090.ENSMUSP00000007255

PTM databases

iPTMnetiQ99LD8
PhosphoSitePlusiQ99LD8

2D gel databases

REPRODUCTION-2DPAGEiIPI00336881
UCD-2DPAGEiQ99LD8

Proteomic databases

EPDiQ99LD8
MaxQBiQ99LD8
PaxDbiQ99LD8
PeptideAtlasiQ99LD8
PRIDEiQ99LD8

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000007255; ENSMUSP00000007255; ENSMUSG00000007039
GeneIDi51793
KEGGimmu:51793
UCSCiuc008cfi.2 mouse

Organism-specific databases

CTDi23564
MGIiMGI:1859016 Ddah2

Phylogenomic databases

eggNOGiENOG410ISNS Eukaryota
ENOG4111YMV LUCA
GeneTreeiENSGT00390000009331
HOGENOMiHOG000161035
HOVERGENiHBG055937
InParanoidiQ99LD8
KOiK01482
OMAiAQRQFGC
OrthoDBiEOG091G0M5S
PhylomeDBiQ99LD8
TreeFamiTF314737

Enzyme and pathway databases

BRENDAi3.5.3.18 3474
ReactomeiR-MMU-203615 eNOS activation

Miscellaneous databases

PROiPR:Q99LD8
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000007039 Expressed in 254 organ(s), highest expression level in cerebral cortex
CleanExiMM_DDAH2
ExpressionAtlasiQ99LD8 baseline and differential
GenevisibleiQ99LD8 MM

Family and domain databases

InterProiView protein in InterPro
IPR033199 DDAH/AD
IPR033202 DDAH2
PANTHERiPTHR12737 PTHR12737, 1 hit
PTHR12737:SF16 PTHR12737:SF16, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDDAH2_MOUSE
AccessioniPrimary (citable) accession number: Q99LD8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: June 1, 2001
Last modified: November 7, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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