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Protein

Eukaryotic translation initiation factor 2 subunit 2

Gene

Eif2s2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri279 – 303C4-typeSequence analysisAdd BLAST25

GO - Molecular functioni

GO - Biological processi

  • in utero embryonic development Source: MGI
  • male germ cell proliferation Source: MGI
  • male gonad development Source: MGI

Keywordsi

Molecular functionInitiation factor
Biological processProtein biosynthesis
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-382556 ABC-family proteins mediated transport
R-MMU-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-MMU-72702 Ribosomal scanning and start codon recognition
R-MMU-72731 Recycling of eIF2:GDP

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 2 subunit 2
Alternative name(s):
Eukaryotic translation initiation factor 2 subunit beta
Short name:
eIF-2-beta
Gene namesi
Name:Eif2s2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1914454 Eif2s2

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001374072 – 331Eukaryotic translation initiation factor 2 subunit 2Add BLAST330

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei2PhosphoserineBy similarity1
Modified residuei13PhosphoserineBy similarity1
Modified residuei36PhosphothreonineBy similarity1
Modified residuei67PhosphoserineCombined sources1
Cross-linki102Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei105PhosphoserineCombined sources1
Modified residuei158PhosphoserineBy similarity1
Modified residuei216PhosphoserineBy similarity1
Modified residuei263N6-acetyllysineBy similarity1
Modified residuei291N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ99L45
MaxQBiQ99L45
PaxDbiQ99L45
PRIDEiQ99L45

PTM databases

iPTMnetiQ99L45
PhosphoSitePlusiQ99L45
SwissPalmiQ99L45

Expressioni

Gene expression databases

BgeeiENSMUSG00000074656 Expressed in 294 organ(s), highest expression level in embryonic stem cell
ExpressionAtlasiQ99L45 baseline and differential
GenevisibleiQ99L45 MM

Interactioni

Subunit structurei

Heterotrimer composed of an alpha, a beta and a gamma chain (By similarity). Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5.By similarity1 Publication

Protein-protein interaction databases

BioGridi212014, 3 interactors
IntActiQ99L45, 3 interactors
STRINGi10090.ENSMUSP00000096777

Structurei

3D structure databases

ProteinModelPortaliQ99L45
SMRiQ99L45
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi14 – 21Poly-Lys8
Compositional biasi79 – 87Poly-Lys9
Compositional biasi124 – 129Poly-Lys6

Sequence similaritiesi

Belongs to the eIF-2-beta/eIF-5 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri279 – 303C4-typeSequence analysisAdd BLAST25

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2768 Eukaryota
COG1601 LUCA
GeneTreeiENSGT00390000001804
HOGENOMiHOG000107198
HOVERGENiHBG000927
InParanoidiQ99L45
KOiK03238
OMAiYAQLHAS
OrthoDBiEOG091G0H6A
PhylomeDBiQ99L45
TreeFamiTF101503

Family and domain databases

InterProiView protein in InterPro
IPR002735 Transl_init_fac_IF2/IF5
IPR016189 Transl_init_fac_IF2/IF5_N
IPR016190 Transl_init_fac_IF2/IF5_Zn-bd
PfamiView protein in Pfam
PF01873 eIF-5_eIF-2B, 1 hit
SMARTiView protein in SMART
SM00653 eIF2B_5, 1 hit
SUPFAMiSSF100966 SSF100966, 1 hit
SSF75689 SSF75689, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.iShow all

Q99L45-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSGDEMIFDP TMSKKKKKKK KPFMLDEEGD AQTEETQPSE TKEVEPEPTE
60 70 80 90 100
EKDVDADEED SRKKDASDDL DDLNFFNQKK KKKKTKKIFD IDEAEEAIKD
110 120 130 140 150
VKIESDAQEP AEPEDDLDIM LGNKKKKKKN VKFPEEDEIL EKDEALEDED
160 170 180 190 200
SKKDDGISFS SQTAWAGSER DYTYEELLNR VFNIMREKNP DMVAGEKRKF
210 220 230 240 250
VMKPPQVVRV GTKKTSFVNF TDICKLLHRQ PKHLLAFLLA ELGTSGSIDG
260 270 280 290 300
NNQLVIKGRF QQKQIENVLR RYIKEYVTCH TCRSPDTILQ KDTRLYFLQC
310 320 330
ETCHSRCSVA SIKTGFQAVT GKRAQLRAKA N
Length:331
Mass (Da):38,092
Last modified:June 1, 2001 - v1
Checksum:iE91BEBBF330B7A5C
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E0CXJ3E0CXJ3_MOUSE
Eukaryotic translation initiation f...
Eif2s2
118Annotation score:

Sequence cautioni

The sequence BAB28490 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK011503 mRNA Translation: BAB27663.1
AK012817 mRNA Translation: BAB28490.2 Different initiation.
BC003848 mRNA Translation: AAH03848.1
CCDSiCCDS38292.1
RefSeqiNP_080306.1, NM_026030.2
UniGeneiMm.377134
Mm.470088

Genome annotation databases

EnsembliENSMUST00000099173; ENSMUSP00000096777; ENSMUSG00000074656
ENSMUST00000166171; ENSMUSP00000128257; ENSMUSG00000074656
GeneIDi67204
KEGGimmu:67204
UCSCiuc008njw.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK011503 mRNA Translation: BAB27663.1
AK012817 mRNA Translation: BAB28490.2 Different initiation.
BC003848 mRNA Translation: AAH03848.1
CCDSiCCDS38292.1
RefSeqiNP_080306.1, NM_026030.2
UniGeneiMm.377134
Mm.470088

3D structure databases

ProteinModelPortaliQ99L45
SMRiQ99L45
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi212014, 3 interactors
IntActiQ99L45, 3 interactors
STRINGi10090.ENSMUSP00000096777

PTM databases

iPTMnetiQ99L45
PhosphoSitePlusiQ99L45
SwissPalmiQ99L45

Proteomic databases

EPDiQ99L45
MaxQBiQ99L45
PaxDbiQ99L45
PRIDEiQ99L45

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000099173; ENSMUSP00000096777; ENSMUSG00000074656
ENSMUST00000166171; ENSMUSP00000128257; ENSMUSG00000074656
GeneIDi67204
KEGGimmu:67204
UCSCiuc008njw.1 mouse

Organism-specific databases

CTDi8894
MGIiMGI:1914454 Eif2s2

Phylogenomic databases

eggNOGiKOG2768 Eukaryota
COG1601 LUCA
GeneTreeiENSGT00390000001804
HOGENOMiHOG000107198
HOVERGENiHBG000927
InParanoidiQ99L45
KOiK03238
OMAiYAQLHAS
OrthoDBiEOG091G0H6A
PhylomeDBiQ99L45
TreeFamiTF101503

Enzyme and pathway databases

ReactomeiR-MMU-382556 ABC-family proteins mediated transport
R-MMU-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-MMU-72702 Ribosomal scanning and start codon recognition
R-MMU-72731 Recycling of eIF2:GDP

Miscellaneous databases

PROiPR:Q99L45
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000074656 Expressed in 294 organ(s), highest expression level in embryonic stem cell
ExpressionAtlasiQ99L45 baseline and differential
GenevisibleiQ99L45 MM

Family and domain databases

InterProiView protein in InterPro
IPR002735 Transl_init_fac_IF2/IF5
IPR016189 Transl_init_fac_IF2/IF5_N
IPR016190 Transl_init_fac_IF2/IF5_Zn-bd
PfamiView protein in Pfam
PF01873 eIF-5_eIF-2B, 1 hit
SMARTiView protein in SMART
SM00653 eIF2B_5, 1 hit
SUPFAMiSSF100966 SSF100966, 1 hit
SSF75689 SSF75689, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiIF2B_MOUSE
AccessioniPrimary (citable) accession number: Q99L45
Secondary accession number(s): Q9CSH6, Q9CT12
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 13, 2001
Last sequence update: June 1, 2001
Last modified: September 12, 2018
This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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