UniProtKB - Q99L43 (CDS2_MOUSE)
Protein
Phosphatidate cytidylyltransferase 2
Gene
Cds2
Organism
Mus musculus (Mouse)
Status
Functioni
Catalyzes the conversion of phosphatidic acid (PA) to CDP-diacylglycerol (CDP-DAG), an essential intermediate in the synthesis of phosphatidylglycerol, cardiolipin and phosphatidylinositol (By similarity). Exhibits specificity for the nature of the acyl chains at the sn-1 and sn-2 positions in the substrate, PA and the preferred acyl chain composition is 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid (By similarity). Plays an important role in regulating the growth and maturation of lipid droplets which are storage organelles at the center of lipid and energy homeostasis (PubMed:26946540).By similarity1 Publication
Catalytic activityi
- a 1,2-diacyl-sn-glycero-3-phosphate + CTP + H+ = a CDP-1,2-diacyl-sn-glycerol + diphosphateBy similarityEC:2.7.7.41By similarityThis reaction proceeds in the forwardBy similarity direction.
- 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphate + CTP + H+ = 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-cytidine-5ʼ-diphosphate + diphosphateBy similarityThis reaction proceeds in the forwardBy similarity direction.
- 1-octadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphate + CTP + H+ = 1-octadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-cytidine-5ʼ-diphosphate + diphosphateBy similarityThis reaction proceeds in the forwardBy similarity direction.
- 1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphate + CTP + H+ = 1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-cytidine-5ʼ-diphosphate + diphosphateBy similarityThis reaction proceeds in the forwardBy similarity direction.
- 1,2-di-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phosphate + CTP + H+ = 1,2-di-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-cytidine-5ʼ-diphosphate + diphosphateBy similarityThis reaction proceeds in the forwardBy similarity direction.
- 1-octadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphate + CTP + H+ = 1-octadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-cytidine-5ʼ-diphosphate + diphosphateBy similarityThis reaction proceeds in the forwardBy similarity direction.
- 1-octadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphate + CTP + H+ = 1-octadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-cytidine-5ʼ-diphosphate + diphosphateBy similarityThis reaction proceeds in the forwardBy similarity direction.
- 1,2-di-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphate + CTP + H+ = 1,2-di-(9Z,12Z-octadecadienoyl)-sn-glycero-3-cytidine-5ʼ-diphosphate + diphosphateBy similarityThis reaction proceeds in the forwardBy similarity direction.
- 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphate + CTP + H+ = 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-cytidine-5ʼ-diphosphate + diphosphateBy similarityThis reaction proceeds in the forwardBy similarity direction.
: CDP-diacylglycerol biosynthesis Pathwayi
This protein is involved in step 3 of the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate.Proteins known to be involved in the 3 steps of the subpathway in this organism are:
- Glycerol-3-phosphate acyltransferase 1, mitochondrial (Gpam), Glycerol-3-phosphate acyltransferase 2, mitochondrial (Gpat2), Glycerol-3-phosphate acyltransferase 4 (Gpat4), Glycerol-3-phosphate acyltransferase 3 (Gpat3), Glycerol-3-phosphate acyltransferase 1, mitochondrial (Gpam)
- Lysocardiolipin acyltransferase 1 (Lclat1), 1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon (Agpat5), 1-acyl-sn-glycerol-3-phosphate acyltransferase beta (Agpat2), 1-acyl-sn-glycerol-3-phosphate acyltransferase gamma (Agpat3), 1-acyl-sn-glycerol-3-phosphate acyltransferase alpha (Agpat1), 1-acyl-sn-glycerol-3-phosphate acyltransferase delta (Agpat4)
- Phosphatidate cytidylyltransferase (Cds2), Phosphatidate cytidylyltransferase, mitochondrial (Tamm41), CDP-diacylglycerol synthase (Tamm41), Phosphatidate cytidylyltransferase, mitochondrial (Tamm41), CDP-diacylglycerol synthase (Tamm41), Phosphatidate cytidylyltransferase (Cds2), Phosphatidate cytidylyltransferase (Cds2), Phosphatidate cytidylyltransferase 1 (Cds1), CDP-diacylglycerol synthase (Tamm41), Phosphatidate cytidylyltransferase 2 (Cds2), Phosphatidate cytidylyltransferase (Cds2), Phosphatidate cytidylyltransferase (Cds2)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate, the pathway CDP-diacylglycerol biosynthesis and in Phospholipid metabolism.
GO - Molecular functioni
- phosphatidate cytidylyltransferase activity Source: UniProtKB
GO - Biological processi
- CDP-diacylglycerol biosynthetic process Source: UniProtKB
- glycosylation Source: BHF-UCL
- lipid droplet formation Source: UniProtKB
- phototransduction Source: BHF-UCL
Keywordsi
Molecular function | Nucleotidyltransferase, Transferase |
Biological process | Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism |
Enzyme and pathway databases
BRENDAi | 2.7.7.41, 3474 |
Reactomei | R-MMU-1483148, Synthesis of PG |
UniPathwayi | UPA00557;UER00614 |
Names & Taxonomyi
Protein namesi | Recommended name: Phosphatidate cytidylyltransferase 2Curated (EC:2.7.7.41By similarity)Alternative name(s): CDP-DAG synthase 2 CDP-DG synthase 2 CDP-diacylglycerol synthase 2 Short name: CDS 2 CDP-diglyceride pyrophosphorylase 2 CDP-diglyceride synthase 2 CTP:phosphatidate cytidylyltransferase 2 |
Gene namesi | Name:Cds2Imported |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1332236, Cds2 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein Sequence analysis
Endoplasmic reticulum
- endoplasmic reticulum Source: MGI
- endoplasmic reticulum membrane Source: GO_Central
Other locations
- integral component of membrane Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 78 – 98 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 129 – 149 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 165 – 185 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 212 – 232 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 261 – 281 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 339 – 359 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000090717 | 1 – 444 | Phosphatidate cytidylyltransferase 2Add BLAST | 444 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 20 | PhosphoserineBy similarity | 1 | |
Modified residuei | 30 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 32 | PhosphoserineCombined sources | 1 | |
Modified residuei | 34 | PhosphoserineBy similarity | 1 | |
Modified residuei | 36 | PhosphoserineCombined sources | 1 | |
Modified residuei | 50 | PhosphothreonineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q99L43 |
jPOSTi | Q99L43 |
MaxQBi | Q99L43 |
PaxDbi | Q99L43 |
PeptideAtlasi | Q99L43 |
PRIDEi | Q99L43 |
ProteomicsDBi | 281301 |
PTM databases
GlyConnecti | 2581, 1 N-Linked glycan (1 site) |
GlyGeni | Q99L43, 1 site |
iPTMneti | Q99L43 |
PhosphoSitePlusi | Q99L43 |
SwissPalmi | Q99L43 |
Expressioni
Tissue specificityi
Ubiquitous. Expressed in the ganglion cell layer and inner nuclear layer of the retina.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000058793, Expressed in substantia nigra and 313 other tissues |
ExpressionAtlasi | Q99L43, baseline and differential |
Genevisiblei | Q99L43, MM |
Interactioni
Subunit structurei
Homodimer.
By similarityProtein-protein interaction databases
BioGRIDi | 226008, 6 interactors |
IntActi | Q99L43, 1 interactor |
MINTi | Q99L43 |
STRINGi | 10090.ENSMUSP00000099470 |
Miscellaneous databases
RNActi | Q99L43, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the CDS family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1440, Eukaryota |
GeneTreei | ENSGT00940000158877 |
InParanoidi | Q99L43 |
OrthoDBi | 1072976at2759 |
PhylomeDBi | Q99L43 |
TreeFami | TF313464 |
Family and domain databases
InterProi | View protein in InterPro IPR000374, PC_trans IPR016720, PC_Trfase_euk |
PANTHERi | PTHR13773, PTHR13773, 1 hit |
PIRSFi | PIRSF018269, PC_trans_euk, 1 hit |
PROSITEi | View protein in PROSITE PS01315, CDS, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All
Q99L43-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTELRQRVVR EDAPPEDKES ESEAKLDGET ASDSESRAET APLPTSVDDT
60 70 80 90 100
PEVLNRALSN LSSRWKNWWV RGILTLAMIA FFFIIIYLGP MVLMMIVMCV
110 120 130 140 150
QIKCFHEIIT IGYNVYHSYD LPWFRTLSWY FLLCVNYFFY GETVTDYFFT
160 170 180 190 200
LVQREEPLRI LSKYHRFISF ALYLTGFCMF VLSLVKKHYR LQFYMFGWTH
210 220 230 240 250
VTLLIVVTQS HLVIHNLFEG MIWFIVPISC VICNDIMAYM FGFFFGRTPL
260 270 280 290 300
IKLSPKKTWE GFIGGFFATV VFGLLLSYVM SGYRCFVCPV EYNNDTNSFT
310 320 330 340 350
VDCEPSDLFR LQEYNIPGVI QSAIGWKTVR MYPFQIHSIA LSTFASLIGP
360 370 380 390 400
FGGFFASGFK RAFKIKDFAN TIPGHGGIMD RFDCQYLMAT FVNVYIASFI
410 420 430 440
RGPNPSKLIQ QFLTLRPDQQ LHIFNTLKSH LTDKGILTSA LEDE
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA2AMQ5 | A2AMQ5_MOUSE | Phosphatidate cytidylyltransferase | Cds2 | 427 | Annotation score: | ||
F6S4G2 | F6S4G2_MOUSE | Phosphatidate cytidylyltransferase | Cds2 | 194 | Annotation score: | ||
Q6PBC0 | Q6PBC0_MOUSE | Phosphatidate cytidylyltransferase | Cds2 | 129 | Annotation score: | ||
H3BLA1 | H3BLA1_MOUSE | Phosphatidate cytidylyltransferase | Cds2 | 33 | Annotation score: | ||
H3BKR7 | H3BKR7_MOUSE | Phosphatidate cytidylyltransferase ... | Cds2 | 56 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 43 | L → P in AAH69879 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY159802 mRNA Translation: AAO17790.1 AK036328 mRNA Translation: BAC29384.1 AK147541 mRNA Translation: BAE27984.1 AK166001 mRNA Translation: BAE38511.1 BC003852 mRNA Translation: AAH03852.1 BC069879 mRNA Translation: AAH69879.1 |
CCDSi | CCDS16772.1 |
RefSeqi | NP_001277968.1, NM_001291039.1 NP_619592.1, NM_138651.7 |
Genome annotation databases
Ensembli | ENSMUST00000103181; ENSMUSP00000099470; ENSMUSG00000058793 |
GeneIDi | 110911 |
KEGGi | mmu:110911 |
UCSCi | uc008mmo.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY159802 mRNA Translation: AAO17790.1 AK036328 mRNA Translation: BAC29384.1 AK147541 mRNA Translation: BAE27984.1 AK166001 mRNA Translation: BAE38511.1 BC003852 mRNA Translation: AAH03852.1 BC069879 mRNA Translation: AAH69879.1 |
CCDSi | CCDS16772.1 |
RefSeqi | NP_001277968.1, NM_001291039.1 NP_619592.1, NM_138651.7 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 226008, 6 interactors |
IntActi | Q99L43, 1 interactor |
MINTi | Q99L43 |
STRINGi | 10090.ENSMUSP00000099470 |
PTM databases
GlyConnecti | 2581, 1 N-Linked glycan (1 site) |
GlyGeni | Q99L43, 1 site |
iPTMneti | Q99L43 |
PhosphoSitePlusi | Q99L43 |
SwissPalmi | Q99L43 |
Proteomic databases
EPDi | Q99L43 |
jPOSTi | Q99L43 |
MaxQBi | Q99L43 |
PaxDbi | Q99L43 |
PeptideAtlasi | Q99L43 |
PRIDEi | Q99L43 |
ProteomicsDBi | 281301 |
Protocols and materials databases
Antibodypediai | 8142, 144 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000103181; ENSMUSP00000099470; ENSMUSG00000058793 |
GeneIDi | 110911 |
KEGGi | mmu:110911 |
UCSCi | uc008mmo.2, mouse |
Organism-specific databases
CTDi | 8760 |
MGIi | MGI:1332236, Cds2 |
Phylogenomic databases
eggNOGi | KOG1440, Eukaryota |
GeneTreei | ENSGT00940000158877 |
InParanoidi | Q99L43 |
OrthoDBi | 1072976at2759 |
PhylomeDBi | Q99L43 |
TreeFami | TF313464 |
Enzyme and pathway databases
UniPathwayi | UPA00557;UER00614 |
BRENDAi | 2.7.7.41, 3474 |
Reactomei | R-MMU-1483148, Synthesis of PG |
Miscellaneous databases
BioGRID-ORCSi | 110911, 21 hits in 55 CRISPR screens |
ChiTaRSi | Cds2, mouse |
PROi | PR:Q99L43 |
RNActi | Q99L43, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000058793, Expressed in substantia nigra and 313 other tissues |
ExpressionAtlasi | Q99L43, baseline and differential |
Genevisiblei | Q99L43, MM |
Family and domain databases
InterProi | View protein in InterPro IPR000374, PC_trans IPR016720, PC_Trfase_euk |
PANTHERi | PTHR13773, PTHR13773, 1 hit |
PIRSFi | PIRSF018269, PC_trans_euk, 1 hit |
PROSITEi | View protein in PROSITE PS01315, CDS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CDS2_MOUSE | |
Accessioni | Q99L43Primary (citable) accession number: Q99L43 Secondary accession number(s): Q3TMD1, Q6NSU1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2005 |
Last sequence update: | June 1, 2001 | |
Last modified: | April 7, 2021 | |
This is version 148 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families