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Protein

Cyclin-G-associated kinase

Gene

Gak

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1 (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei69ATPPROSITE-ProRule annotation1
Active sitei173Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi46 – 54ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell development Source: MGI
  • clathrin coat disassembly Source: MGI
  • clathrin-coated pit assembly Source: MGI
  • clathrin-dependent endocytosis Source: MGI
  • endoplasmic reticulum organization Source: MGI
  • epidermal cell differentiation Source: MGI
  • establishment of skin barrier Source: MGI
  • forebrain morphogenesis Source: MGI
  • Golgi organization Source: MGI
  • Golgi to lysosome transport Source: MGI
  • intrahepatic bile duct development Source: MGI
  • negative regulation of neuron projection development Source: ParkinsonsUK-UCL
  • positive regulation of neural precursor cell proliferation Source: MGI
  • protein localization to Golgi apparatus Source: MGI
  • protein localization to plasma membrane Source: MGI
  • receptor-mediated endocytosis Source: MGI

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processCell cycle
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-G-associated kinase (EC:2.7.11.1)
Gene namesi
Name:Gak
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:2442153 Gak

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cytoplasm, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000859592 – 1305Cyclin-G-associated kinaseAdd BLAST1304

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei2PhosphoserineBy similarity1
Modified residuei16PhosphoserineBy similarity1
Modified residuei454PhosphoserineBy similarity1
Modified residuei768PhosphoserineBy similarity1
Modified residuei774PhosphothreonineBy similarity1
Modified residuei781PhosphoserineBy similarity1
Modified residuei792PhosphothreonineBy similarity1
Modified residuei809PhosphoserineBy similarity1
Modified residuei824PhosphoserineCombined sources1
Modified residuei827PhosphoserineCombined sources1
Modified residuei938PhosphoserineBy similarity1
Modified residuei1122Omega-N-methylarginineCombined sources1
Modified residuei1171PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ99KY4
PRIDEiQ99KY4

PTM databases

iPTMnetiQ99KY4
PhosphoSitePlusiQ99KY4
SwissPalmiQ99KY4

Miscellaneous databases

PMAP-CutDBiQ99KY4

Expressioni

Gene expression databases

CleanExiMM_GAK

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
Il12rb2P973787EBI-7652906,EBI-6253448

GO - Molecular functioni

Protein-protein interaction databases

BioGridi231137, 2 interactors
IntActiQ99KY4, 2 interactors
MINTiQ99KY4
STRINGi10090.ENSMUSP00000036705

Structurei

3D structure databases

ProteinModelPortaliQ99KY4
SMRiQ99KY4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 317Protein kinasePROSITE-ProRule annotationAdd BLAST278
Domaini397 – 564Phosphatase tensin-typePROSITE-ProRule annotationAdd BLAST168
Domaini570 – 708C2 tensin-typePROSITE-ProRule annotationAdd BLAST139
Domaini1241 – 1305JPROSITE-ProRule annotationAdd BLAST65

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0431 Eukaryota
KOG1989 Eukaryota
KOG2283 Eukaryota
ENOG410Y515 LUCA
HOGENOMiHOG000034235
HOVERGENiHBG004322
InParanoidiQ99KY4
KOiK08855
PhylomeDBiQ99KY4
TreeFamiTF105165

Family and domain databases

CDDicd06257 DnaJ, 1 hit
Gene3Di1.10.287.110, 1 hit
3.90.190.10, 1 hit
InterProiView protein in InterPro
IPR001623 DnaJ_domain
IPR036869 J_dom_sf
IPR011009 Kinase-like_dom_sf
IPR029021 Prot-tyrosine_phosphatase-like
IPR000719 Prot_kinase_dom
IPR008271 Ser/Thr_kinase_AS
IPR014020 Tensin_C2-dom
IPR029023 Tensin_phosphatase
PfamiView protein in Pfam
PF00226 DnaJ, 1 hit
PF00069 Pkinase, 1 hit
PF10409 PTEN_C2, 1 hit
SMARTiView protein in SMART
SM00271 DnaJ, 1 hit
SM01326 PTEN_C2, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF46565 SSF46565, 1 hit
SSF52799 SSF52799, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS51182 C2_TENSIN, 1 hit
PS50076 DNAJ_2, 1 hit
PS51181 PPASE_TENSIN, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q99KY4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLLQSALDF LAGPGSLGGA AGRDQSDFVG QTVELGELRL RVRRVLAEGG
60 70 80 90 100
FAFVYEAQDL GSGREYALKR LLSNEEEKNR AIIQEVCFLK KLSGHPNIVQ
110 120 130 140 150
FCSAASIGKE ESDTGQAEFL LLTELCKGQL VEFLKRVECK GPLSCDSILK
160 170 180 190 200
IFYQTCRAVQ HMHRQKPPII HRDLKVENLL LSNQGTIKLC DFGSATTISH
210 220 230 240 250
YPDYSWSAQK RAMVEEEITR NTTPMYRTPE IVDLYSNFPI GEKQDIWALG
260 270 280 290 300
CILYLLCFRQ HPFEDGAKLR IVNGKYSIPV NDTRYTVFHD LIRAMLKVNP
310 320 330 340 350
VERLSIAEVV RQLQEIAAAR NVNPKAPITE LLEQNGGYGN SGPSRAQPPC
360 370 380 390 400
GGTVNSSGVL ALAEYDQPYG GFLDILRGGT ERLFTNLKDT SSKVIQSVAN
410 420 430 440 450
YAKGDLDISY ITSRIAVMSF PAEGVESAIK NNIEDVRMFL DAKHPGHYAV
460 470 480 490 500
YNLSPRIYRA SKFHNRVTEC GWAVRRAPHL HSLYTLCRSM HAWLREDHRN
510 520 530 540 550
VCVVHCMDGR AASAVAVCAF LCFCRLFSTA EAAVYMFSMK RCPPGIWPSH
560 570 580 590 600
KRYIEYVCDM VAEEPITPHS KPMLVKSVVM TPVPLFSKQR NGCRPFCEVY
610 620 630 640 650
VGEERVTTTS QEYDRMKEFK IEDGKAVIPL GVTVQGDVLI IIYHARATLG
660 670 680 690 700
GRLQAKMASM KMFQIQFHTG FVPRNATTVK FAKYDLDACD IQEKYPDLFQ
710 720 730 740 750
VNLEVEVEPR DRPSREAPPW ENTSLRGLNP KILFSNREEQ QDILSKFGKP
760 770 780 790 800
ELPRQPGSTA QYDAEAGSPE AEITESDSPQ SSSTDTNHFL HTLDWQEEKE
810 820 830 840 850
PETGLDNTSP KESQSVLIAD GDGSEVSDEE EASFPSEERK PGAGEDTPRL
860 870 880 890 900
AAGTKQQDLI FDVGMLAAPQ EPVQPEEGVD LLGLHSEGDL RPAAPLQACG
910 920 930 940 950
VPSSNTDLLS CLLEPSDAAQ VGPPGDLLGG EAPLLLASPV SPLGLQNNLQ
960 970 980 990 1000
GKVPDTVDPF DQFLLSSNSD TQPCSKPDLF GEFLNSDSVA SSTAFPSTHS
1010 1020 1030 1040 1050
APPPSCSTAF LHLGDLPAEP SKVIASSSHP DLLGGWDTWA DTATPGPASI
1060 1070 1080 1090 1100
PVPEGTLFSS AGHPAPPGPN PSQTKSQNLD PFADLSDLSS SLQGLPAGLP
1110 1120 1130 1140 1150
AGGFVGAPAP TQKSNSPWQA NRPTAPGTSW TPQAKPAPRA SEQLRSHFSV
1160 1170 1180 1190 1200
IGAREERGVR VPSFAQKPKV SENDFEDLLP NQGFSKSDKK GPKTMAEMRK
1210 1220 1230 1240 1250
QELARDTDPL KLKLLDWIEG KERNIRALLS TLHTVLWDGE SRWTPVSMAD
1260 1270 1280 1290 1300
LVTPEQVKKQ YRRAVLVVHP DKATGQPYEQ YAKMIFMELN DAWSEFENQG

SRPLF
Length:1,305
Mass (Da):143,641
Last modified:October 11, 2005 - v2
Checksum:i4FC416FE52650ED2
GO
Isoform 2 (identifier: Q99KY4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     748-796: Missing.

Note: No experimental confirmation available.
Show »
Length:1,256
Mass (Da):138,344
Checksum:i47F3767646BDF6C7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti301V → E in BAE38941 (PubMed:16141072).Curated1
Sequence conflicti301V → E in AAH65052 (PubMed:15489334).Curated1
Sequence conflicti822D → G in BAC28779 (PubMed:16141072).Curated1
Sequence conflicti868A → V in AAH03958 (PubMed:15489334).Curated1
Sequence conflicti868A → V in AAH30859 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_015821748 – 796Missing in isoform 2. 1 PublicationAdd BLAST49

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK034637 mRNA Translation: BAC28779.1
AK166682 mRNA Translation: BAE38941.1
BC003958 mRNA Translation: AAH03958.1
BC030859 mRNA Translation: AAH30859.1
BC060622 mRNA Translation: AAH60622.1
BC065052 mRNA Translation: AAH65052.1
CCDSiCCDS19513.1 [Q99KY4-1]
RefSeqiNP_001268980.1, NM_001282051.1
NP_001268981.1, NM_001282052.1
NP_705797.1, NM_153569.2
UniGeneiMm.276647

Genome annotation databases

GeneIDi231580
KEGGimmu:231580
UCSCiuc008yoh.2 mouse [Q99KY4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiGAK_MOUSE
AccessioniPrimary (citable) accession number: Q99KY4
Secondary accession number(s): Q6P1I8
, Q6P9S5, Q8BM74, Q8K0Q4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: October 11, 2005
Last modified: June 20, 2018
This is version 151 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

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