UniProtKB - Q99KV1 (DJB11_MOUSE)
Protein
DnaJ homolog subfamily B member 11
Gene
Dnajb11
Organism
Mus musculus (Mouse)
Status
Functioni
As a co-chaperone for HSPA5 it is required for proper folding, trafficking or degradation of proteins. Binds directly to both unfolded proteins that are substrates for ERAD and nascent unfolded peptide chains, but dissociates from the HSPA5-unfolded protein complex before folding is completed. May help recruiting HSPA5 and other chaperones to the substrate. Stimulates HSPA5 ATPase activity. It is necessary for maturation and correct trafficking of PKD1.By similarity
Caution
PubMed:11584023 reported a cytosolic, as well as nuclear subcellular location. This result was obtained using an N-terminally GFP-tagged construct which most probably affected signal peptide-driven targeting to the ER. As a consequence, the in vivo revelance of the observed interaction with APOBEC1, a nuclear protein, is dubious.Curated
GO - Molecular functioni
- signaling receptor binding Source: MGI
- unfolded protein binding Source: MGI
GO - Biological processi
- mRNA modification Source: MGI
- negative regulation of neurogenesis Source: MGI
- positive regulation of ATPase activity Source: MGI
- protein folding Source: InterPro
- protein maturation Source: UniProtKB
Keywordsi
Molecular function | Chaperone |
Names & Taxonomyi
Protein namesi | Recommended name: DnaJ homolog subfamily B member 11Alternative name(s): APOBEC1-binding protein 2 Short name: ABBP-2 ER-associated DNAJ ER-associated Hsp40 co-chaperone Endoplasmic reticulum DNA J domain-containing protein 3 Short name: ER-resident protein ERdj3 Short name: ERdj3 Short name: ERj3p |
Gene namesi | Name:Dnajb11 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1915088, Dnajb11 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum lumen 1 Publication
Endoplasmic reticulum
- endoplasmic reticulum Source: MGI
- endoplasmic reticulum chaperone complex Source: MGI
- endoplasmic reticulum lumen Source: UniProtKB-SubCell
Extracellular region or secreted
- extracellular space Source: MGI
Nucleus
- nucleus Source: MGI
Other locations
- cytoplasm Source: MGI
Keywords - Cellular componenti
Endoplasmic reticulumPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 22 | By similarityAdd BLAST | 22 | |
ChainiPRO_0000007261 | 23 – 358 | DnaJ homolog subfamily B member 11Add BLAST | 336 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 188 | PhosphothreonineBy similarity | 1 | |
Glycosylationi | 261 | N-linked (GlcNAc...) asparagineCurated | 1 |
Post-translational modificationi
Contains high-mannose Endo H-sensitive carbohydrates.By similarity
Cys-169, Cys-171, Cys-193 and Cys-196 form intramolecular disulfide bonds. The preferential partner for each Cys is not known (By similarity).By similarity
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
EPDi | Q99KV1 |
jPOSTi | Q99KV1 |
MaxQBi | Q99KV1 |
PaxDbi | Q99KV1 |
PeptideAtlasi | Q99KV1 |
PRIDEi | Q99KV1 |
2D gel databases
REPRODUCTION-2DPAGEi | Q99KV1 |
PTM databases
GlyGeni | Q99KV1, 1 site |
iPTMneti | Q99KV1 |
PhosphoSitePlusi | Q99KV1 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000004460, Expressed in pancreas and 310 other tissues |
ExpressionAtlasi | Q99KV1, baseline and differential |
Genevisiblei | Q99KV1, MM |
Interactioni
Subunit structurei
Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGGT1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Binds to denatured substrates in an ATP-independent manner.
Interacts via the J domain with HSPA5 in an ATP-dependent manner (By similarity).
By similarityBinary interactionsi
Q99KV1
With | #Exp. | IntAct |
---|---|---|
Hspa5 [P20029] | 4 | EBI-8328260,EBI-772325 |
GO - Molecular functioni
- signaling receptor binding Source: MGI
- unfolded protein binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 212468, 23 interactors |
CORUMi | Q99KV1 |
DIPi | DIP-60531N |
IntActi | Q99KV1, 10 interactors |
MINTi | Q99KV1 |
STRINGi | 10090.ENSMUSP00000126828 |
Miscellaneous databases
RNActi | Q99KV1, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 25 – 90 | JPROSITE-ProRule annotationAdd BLAST | 66 |
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG0713, Eukaryota |
GeneTreei | ENSGT00940000155792 |
HOGENOMi | CLU_017633_0_0_1 |
InParanoidi | Q99KV1 |
OMAi | KEGMMDH |
OrthoDBi | 687505at2759 |
PhylomeDBi | Q99KV1 |
TreeFami | TF105144 |
Family and domain databases
CDDi | cd06257, DnaJ, 1 hit |
Gene3Di | 1.10.287.110, 1 hit |
InterProi | View protein in InterPro IPR002939, DnaJ_C IPR001623, DnaJ_domain IPR018253, DnaJ_domain_CS IPR008971, HSP40/DnaJ_pept-bd IPR036869, J_dom_sf |
Pfami | View protein in Pfam PF00226, DnaJ, 1 hit PF01556, DnaJ_C, 1 hit |
PRINTSi | PR00625, JDOMAIN |
SMARTi | View protein in SMART SM00271, DnaJ, 1 hit |
SUPFAMi | SSF46565, SSF46565, 1 hit SSF49493, SSF49493, 2 hits |
PROSITEi | View protein in PROSITE PS00636, DNAJ_1, 1 hit PS50076, DNAJ_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q99KV1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAPQNLSTFC LLLLYLIGTV IAGRDFYKIL GVPRSASIKD IKKAYRKLAL
60 70 80 90 100
QLHPDRNPDD PQAQEKFQDL GAAYEVLSDS EKRKQYDTYG EEGLKDGHQS
110 120 130 140 150
SHGDIFSHFF GDFGFMFGGT PRQQDRNIPR GSDIIVDLEV TLEEVYAGNF
160 170 180 190 200
VEVVRNKPVA RQAPGKRKCN CRQEMRTTQL GPGRFQMTQE VVCDECPNVK
210 220 230 240 250
LVNEERTLEV EIEPGVRDGM EYPFIGEGEP HVDGEPGDLR FRIKVVKHRI
260 270 280 290 300
FERRGDDLYT NVTVSLVEAL VGFEMDITHL DGHKVHISRD KITRPGAKLW
310 320 330 340 350
KKGEGLPNFD NNNIKGSLII TFDVDFPKEQ LTEEAKEGIK QLLKQGPVQK
VYNGLQGY
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A338P778 | A0A338P778_MOUSE | DnaJ homolog subfamily B member 11 | Dnajb11 | 127 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 9 | F → L in BAC35956 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 88 | T → A in AAH18282 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 217 | R → L in AAL17676 (PubMed:11584023).Curated | 1 | |
Sequence conflicti | 240 | R → P in AAL17676 (PubMed:11584023).Curated | 1 | |
Sequence conflicti | 255 | G → E in BAC35956 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY054981 mRNA Translation: AAL17676.1 AK050500 mRNA Translation: BAC34293.1 AK075785 mRNA Translation: BAC35956.1 AK075953 mRNA Translation: BAC36079.1 AK148511 mRNA Translation: BAE28593.1 AK166730 mRNA Translation: BAE38977.1 CT027991 Genomic DNA No translation available. BC003999 mRNA Translation: AAH03999.1 BC018282 mRNA Translation: AAH18282.1 BC040747 mRNA Translation: AAH40747.1 |
CCDSi | CCDS28070.1 |
RefSeqi | NP_001177733.1, NM_001190804.1 NP_001177734.1, NM_001190805.1 NP_080676.3, NM_026400.5 |
Genome annotation databases
Ensembli | ENSMUST00000004574; ENSMUSP00000004574; ENSMUSG00000004460 ENSMUST00000166487; ENSMUSP00000126828; ENSMUSG00000004460 ENSMUST00000178320; ENSMUSP00000137542; ENSMUSG00000004460 |
GeneIDi | 67838 |
KEGGi | mmu:67838 |
UCSCi | uc007yso.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY054981 mRNA Translation: AAL17676.1 AK050500 mRNA Translation: BAC34293.1 AK075785 mRNA Translation: BAC35956.1 AK075953 mRNA Translation: BAC36079.1 AK148511 mRNA Translation: BAE28593.1 AK166730 mRNA Translation: BAE38977.1 CT027991 Genomic DNA No translation available. BC003999 mRNA Translation: AAH03999.1 BC018282 mRNA Translation: AAH18282.1 BC040747 mRNA Translation: AAH40747.1 |
CCDSi | CCDS28070.1 |
RefSeqi | NP_001177733.1, NM_001190804.1 NP_001177734.1, NM_001190805.1 NP_080676.3, NM_026400.5 |
3D structure databases
SMRi | Q99KV1 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 212468, 23 interactors |
CORUMi | Q99KV1 |
DIPi | DIP-60531N |
IntActi | Q99KV1, 10 interactors |
MINTi | Q99KV1 |
STRINGi | 10090.ENSMUSP00000126828 |
PTM databases
GlyGeni | Q99KV1, 1 site |
iPTMneti | Q99KV1 |
PhosphoSitePlusi | Q99KV1 |
2D gel databases
REPRODUCTION-2DPAGEi | Q99KV1 |
Proteomic databases
EPDi | Q99KV1 |
jPOSTi | Q99KV1 |
MaxQBi | Q99KV1 |
PaxDbi | Q99KV1 |
PeptideAtlasi | Q99KV1 |
PRIDEi | Q99KV1 |
Genome annotation databases
Ensembli | ENSMUST00000004574; ENSMUSP00000004574; ENSMUSG00000004460 ENSMUST00000166487; ENSMUSP00000126828; ENSMUSG00000004460 ENSMUST00000178320; ENSMUSP00000137542; ENSMUSG00000004460 |
GeneIDi | 67838 |
KEGGi | mmu:67838 |
UCSCi | uc007yso.2, mouse |
Organism-specific databases
CTDi | 51726 |
MGIi | MGI:1915088, Dnajb11 |
Phylogenomic databases
eggNOGi | KOG0713, Eukaryota |
GeneTreei | ENSGT00940000155792 |
HOGENOMi | CLU_017633_0_0_1 |
InParanoidi | Q99KV1 |
OMAi | KEGMMDH |
OrthoDBi | 687505at2759 |
PhylomeDBi | Q99KV1 |
TreeFami | TF105144 |
Miscellaneous databases
BioGRID-ORCSi | 67838, 1 hit in 17 CRISPR screens |
ChiTaRSi | Dnajb11, mouse |
PROi | PR:Q99KV1 |
RNActi | Q99KV1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000004460, Expressed in pancreas and 310 other tissues |
ExpressionAtlasi | Q99KV1, baseline and differential |
Genevisiblei | Q99KV1, MM |
Family and domain databases
CDDi | cd06257, DnaJ, 1 hit |
Gene3Di | 1.10.287.110, 1 hit |
InterProi | View protein in InterPro IPR002939, DnaJ_C IPR001623, DnaJ_domain IPR018253, DnaJ_domain_CS IPR008971, HSP40/DnaJ_pept-bd IPR036869, J_dom_sf |
Pfami | View protein in Pfam PF00226, DnaJ, 1 hit PF01556, DnaJ_C, 1 hit |
PRINTSi | PR00625, JDOMAIN |
SMARTi | View protein in SMART SM00271, DnaJ, 1 hit |
SUPFAMi | SSF46565, SSF46565, 1 hit SSF49493, SSF49493, 2 hits |
PROSITEi | View protein in PROSITE PS00636, DNAJ_1, 1 hit PS50076, DNAJ_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DJB11_MOUSE | |
Accessioni | Q99KV1Primary (citable) accession number: Q99KV1 Secondary accession number(s): A6X957 Q8VHB1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 18, 2001 |
Last sequence update: | June 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 165 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot