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Protein

Dehydrodolichyl diphosphate synthase complex subunit Dhdds

Gene

Dhdds

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

With NUS1, forms the dehydrodolichyl diphosphate synthase (DDS) complex, an essential component of the dolichol monophosphate (Dol-P) biosynthetic machinery. Both subunits contribute to enzymatic activity, i.e. condensation of multiple copies of isopentenyl pyrophosphate (IPP) to farnesyl pyrophosphate (FPP) to produce dehydrodolichyl diphosphate (Dedol-PP), a precursor of dolichol phosphate which is utilized as a sugar carrier in protein glycosylation in the endoplasmic reticulum (ER). Regulates the glycosylation and stability of nascent NPC2, thereby promoting trafficking of LDL-derived cholesterol.By similarity

Catalytic activityi

(2E,6E)-farnesyl diphosphate + n isopentenyl diphosphate = n diphosphate + ditrans,polycis-polyprenyl diphosphate (n = 10-55).By similarity

Cofactori

Mg2+By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.By similarity
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Pathwayi: Lipid metabolism

This protein is involved in Lipid metabolism.By similarity
View all proteins of this organism that are known to be involved in Lipid metabolism.

GO - Molecular functioni

  • dehydrodolichyl diphosphate synthase activity Source: UniProtKB
  • polyprenyltransferase activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processLipid metabolism
LigandMagnesium

Enzyme and pathway databases

ReactomeiR-MMU-446199 Synthesis of Dolichyl-phosphate
UniPathwayi
UPA00378

Names & Taxonomyi

Protein namesi
Recommended name:
Dehydrodolichyl diphosphate synthase complex subunit DhddsCurated (EC:2.5.1.87By similarity)
Alternative name(s):
Cis-isoprenyltransferaseBy similarity
Short name:
CITBy similarity
Short name:
Cis-IPTaseBy similarity
Gene namesi
Name:DhddsImported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1914672 Dhdds

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001237501 – 333Dehydrodolichyl diphosphate synthase complex subunit DhddsAdd BLAST333

Proteomic databases

EPDiQ99KU1
PaxDbiQ99KU1
PRIDEiQ99KU1

PTM databases

iPTMnetiQ99KU1
PhosphoSitePlusiQ99KU1

Expressioni

Gene expression databases

BgeeiENSMUSG00000012117 Expressed in 264 organ(s), highest expression level in membranous labyrinth
CleanExiMM_DHDDS
ExpressionAtlasiQ99KU1 baseline and differential
GenevisibleiQ99KU1 MM

Interactioni

Subunit structurei

Forms an active dehydrodolichyl diphosphate synthase complex with NUS1. Interacts with NPC2.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000012262

Structurei

3D structure databases

ProteinModelPortaliQ99KU1
SMRiQ99KU1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the UPP synthase family.Curated

Phylogenomic databases

eggNOGiKOG1602 Eukaryota
COG0020 LUCA
GeneTreeiENSGT00390000007879
HOGENOMiHOG000006053
HOVERGENiHBG051350
InParanoidiQ99KU1
KOiK11778
OrthoDBiEOG091G0K4O
PhylomeDBiQ99KU1
TreeFamiTF323753

Family and domain databases

CDDicd00475 Cis_IPPS, 1 hit
Gene3Di3.40.1180.10, 1 hit
HAMAPiMF_01139 ISPT, 1 hit
InterProiView protein in InterPro
IPR001441 UPP_synth-like
IPR018520 UPP_synth-like_CS
IPR036424 UPP_synth-like_sf
PANTHERiPTHR10291 PTHR10291, 1 hit
PfamiView protein in Pfam
PF01255 Prenyltransf, 1 hit
SUPFAMiSSF64005 SSF64005, 1 hit
TIGRFAMsiTIGR00055 uppS, 1 hit
PROSITEiView protein in PROSITE
PS01066 UPP_SYNTHASE, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q99KU1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSWIKEGELS LWERFCANII KAGPVPKHIA FIMDGNRRYA KKCQVERQEG
60 70 80 90 100
HTQGFNKLAE TLRWCLNLGI LEVTVYAFSI ENFKRSKSEV DGLLDLARQK
110 120 130 140 150
FSCLMEEQEK LQKHGVCIRV LGDLHLLPLD LQEKIAHAIQ ATKNYNKCFL
160 170 180 190 200
NVCFAYTSRH EIANAVREMA WGVEQGLLEP SDVSESLLDK CLYSNHSPHP
210 220 230 240 250
DILIRTSGEV RLSDFLLWQT SHSCLVFQPV LWPEYTFWNL CEAILQFQRN
260 270 280 290 300
HGALQKARDM YAEERKRRQL ERDQAAVTEQ LLREGLQASG DAQLRRTRLH
310 320 330
KLSTKREERV QGFLKALELK RANWLALWGT ASA
Length:333
Mass (Da):38,509
Last modified:June 1, 2001 - v1
Checksum:i53992F5E92D3F2C9
GO
Isoform 2 (identifier: Q99KU1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     255-255: Q → QQ

Show »
Length:334
Mass (Da):38,637
Checksum:iB4574B527D0D8C1B
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A3KGL0A3KGL0_MOUSE
Alkyl transferase
Dhdds
299Annotation score:
A3KGL3A3KGL3_MOUSE
Alkyl transferase
Dhdds
258Annotation score:
A3KGL2A3KGL2_MOUSE
Dehydrodolichyl diphosphate synthas...
Dhdds
294Annotation score:
A3KGK9A3KGK9_MOUSE
Dehydrodolichyl diphosphate synthas...
Dhdds
168Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti30A → L in BAC29643 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_010032255Q → QQ in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK034277 mRNA Translation: BAC28657.1
AK036929 mRNA Translation: BAC29643.1
AK049991 mRNA Translation: BAC34020.1
AK135087 mRNA Translation: BAE22416.1
AK149138 mRNA Translation: BAE28748.1
AK171207 mRNA Translation: BAE42313.1
AL837508, AL670680 Genomic DNA Translation: CAM46083.1
BC004011 mRNA Translation: AAH04011.1
CCDSiCCDS18760.1 [Q99KU1-1]
RefSeqiNP_080420.2, NM_026144.4 [Q99KU1-1]
UniGeneiMm.100290

Genome annotation databases

EnsembliENSMUST00000012262; ENSMUSP00000012262; ENSMUSG00000012117 [Q99KU1-1]
ENSMUST00000105887; ENSMUSP00000101511; ENSMUSG00000012117 [Q99KU1-2]
ENSMUST00000144668; ENSMUSP00000116098; ENSMUSG00000012117 [Q99KU1-1]
GeneIDi67422
KEGGimmu:67422
UCSCiuc008vds.1 mouse [Q99KU1-1]
uc008vdv.1 mouse [Q99KU1-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK034277 mRNA Translation: BAC28657.1
AK036929 mRNA Translation: BAC29643.1
AK049991 mRNA Translation: BAC34020.1
AK135087 mRNA Translation: BAE22416.1
AK149138 mRNA Translation: BAE28748.1
AK171207 mRNA Translation: BAE42313.1
AL837508, AL670680 Genomic DNA Translation: CAM46083.1
BC004011 mRNA Translation: AAH04011.1
CCDSiCCDS18760.1 [Q99KU1-1]
RefSeqiNP_080420.2, NM_026144.4 [Q99KU1-1]
UniGeneiMm.100290

3D structure databases

ProteinModelPortaliQ99KU1
SMRiQ99KU1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000012262

PTM databases

iPTMnetiQ99KU1
PhosphoSitePlusiQ99KU1

Proteomic databases

EPDiQ99KU1
PaxDbiQ99KU1
PRIDEiQ99KU1

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000012262; ENSMUSP00000012262; ENSMUSG00000012117 [Q99KU1-1]
ENSMUST00000105887; ENSMUSP00000101511; ENSMUSG00000012117 [Q99KU1-2]
ENSMUST00000144668; ENSMUSP00000116098; ENSMUSG00000012117 [Q99KU1-1]
GeneIDi67422
KEGGimmu:67422
UCSCiuc008vds.1 mouse [Q99KU1-1]
uc008vdv.1 mouse [Q99KU1-2]

Organism-specific databases

CTDi79947
MGIiMGI:1914672 Dhdds

Phylogenomic databases

eggNOGiKOG1602 Eukaryota
COG0020 LUCA
GeneTreeiENSGT00390000007879
HOGENOMiHOG000006053
HOVERGENiHBG051350
InParanoidiQ99KU1
KOiK11778
OrthoDBiEOG091G0K4O
PhylomeDBiQ99KU1
TreeFamiTF323753

Enzyme and pathway databases

UniPathwayi
UPA00378

ReactomeiR-MMU-446199 Synthesis of Dolichyl-phosphate

Miscellaneous databases

PROiPR:Q99KU1
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000012117 Expressed in 264 organ(s), highest expression level in membranous labyrinth
CleanExiMM_DHDDS
ExpressionAtlasiQ99KU1 baseline and differential
GenevisibleiQ99KU1 MM

Family and domain databases

CDDicd00475 Cis_IPPS, 1 hit
Gene3Di3.40.1180.10, 1 hit
HAMAPiMF_01139 ISPT, 1 hit
InterProiView protein in InterPro
IPR001441 UPP_synth-like
IPR018520 UPP_synth-like_CS
IPR036424 UPP_synth-like_sf
PANTHERiPTHR10291 PTHR10291, 1 hit
PfamiView protein in Pfam
PF01255 Prenyltransf, 1 hit
SUPFAMiSSF64005 SSF64005, 1 hit
TIGRFAMsiTIGR00055 uppS, 1 hit
PROSITEiView protein in PROSITE
PS01066 UPP_SYNTHASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDHDDS_MOUSE
AccessioniPrimary (citable) accession number: Q99KU1
Secondary accession number(s): A3KGL4
, Q3UF13, Q8BZ16, Q8BZK8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: June 1, 2001
Last modified: November 7, 2018
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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