UniProtKB - Q99KP6 (PRP19_MOUSE)
Pre-mRNA-processing factor 19
Prpf19
Functioni
Catalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.By similarity EC:2.3.2.27
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.By similarityView all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
GO - Molecular functioni
- identical protein binding Source: MGI
- ubiquitin protein ligase activity Source: UniProtKB
- ubiquitin-protein transferase activity Source: GO_Central
- ubiquitin-ubiquitin ligase activity Source: MGI
GO - Biological processi
- cellular protein localization Source: UniProtKB
- double-strand break repair via nonhomologous end joining Source: UniProtKB
- generation of catalytic spliceosome for first transesterification step Source: GO_Central
- inner cell mass cell proliferation Source: MGI
- lipid biosynthetic process Source: MGI
- mRNA splicing, via spliceosome Source: UniProtKB
- negative regulation of neuron differentiation Source: MGI
- positive regulation of astrocyte differentiation Source: MGI
- positive regulation of mRNA splicing, via spliceosome Source: MGI
- positive regulation of neuron differentiation Source: MGI
- proteasomal protein catabolic process Source: UniProtKB
- protein K63-linked ubiquitination Source: UniProtKB
- protein polyubiquitination Source: MGI
- signal transduction involved in DNA damage checkpoint Source: UniProtKB
- spliceosomal complex assembly Source: MGI
- spliceosomal tri-snRNP complex assembly Source: UniProtKB
Keywordsi
Molecular function | Transferase |
Biological process | DNA damage, DNA repair, mRNA processing, mRNA splicing, Ubl conjugation pathway |
Enzyme and pathway databases
Reactomei | R-MMU-6781823, Formation of TC-NER Pre-Incision Complex R-MMU-6782135, Dual incision in TC-NER R-MMU-6782210, Gap-filling DNA repair synthesis and ligation in TC-NER R-MMU-72163, mRNA Splicing - Major Pathway |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: Pre-mRNA-processing factor 19Curated (EC:2.3.2.27By similarity)Alternative name(s): Nuclear matrix protein 2001 Publication PRP19/PSO4 homolog RING-type E3 ubiquitin transferase PRP19Curated Senescence evasion factor1 Publication |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:106247, Prpf19 |
Subcellular locationi
Cytoskeleton
- spindle By similarity
Nucleus
- Nucleus 1 Publication
- nucleoplasm By similarity
Other locations
- Cytoplasm By similarity
- Lipid droplet 1 Publication
Note: Nucleoplasmic in interphase cells. Irregularly distributed in anaphase cells. In prophase cells, uniformly distributed, but not associated with condensing chromosomes. Found in extrachromosomal regions in metaphase cells. Mainly localized to the mitotic spindle apparatus when chromosomes segregate during anaphase. When nuclei reform during late telophase, uniformly distributed in daughter cells and displays no preferred association with decondensing chromatin. Recruited on damaged DNA at sites of double-strand break (By similarity).By similarity
Cytoskeleton
- spindle Source: UniProtKB-SubCell
Nucleus
- catalytic step 2 spliceosome Source: MGI
- DNA replication factor A complex Source: Ensembl
- nuclear speck Source: UniProtKB
- nucleus Source: MGI
- spliceosomal complex Source: MGI
- U2-type catalytic step 1 spliceosome Source: GO_Central
- U2-type catalytic step 2 spliceosome Source: MGI
Other locations
- cytoplasm Source: MGI
- lipid droplet Source: MGI
- Prp19 complex Source: UniProtKB
- site of double-strand break Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, Lipid droplet, Nucleus, SpliceosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000051146 | 2 – 504 | Pre-mRNA-processing factor 19Add BLAST | 503 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineBy similarity | 1 | |
Modified residuei | 122 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 179 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 244 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 261 | N6-acetyllysineBy similarity | 1 |
Keywords - PTMi
AcetylationProteomic databases
EPDi | Q99KP6 |
jPOSTi | Q99KP6 |
PaxDbi | Q99KP6 |
PeptideAtlasi | Q99KP6 |
PRIDEi | Q99KP6 |
2D gel databases
REPRODUCTION-2DPAGEi | Q99KP6 |
PTM databases
iPTMneti | Q99KP6 |
PhosphoSitePlusi | Q99KP6 |
SwissPalmi | Q99KP6 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000024735, Expressed in supraoptic nucleus and 294 other tissues |
Genevisiblei | Q99KP6, MM |
Interactioni
Subunit structurei
Homotetramer.
Component of activated, catalytic and post-catalytic spliceosomes.
Component of the Prp19 complex/PRP19C/Nineteen complex/NTC and related complexes described as PRP19-CDC5L splicing complex and PSO4 complex. A homotetramer of PRPF19, CDC5L, PLRG1 and BCAS2 constitute the core of those complexes. The interaction with CDC5L, PLRG1 and BCAS2 is direct within this core complex. At least three less stably associated proteins CTNNBL1, CWC15 and HSPA8 are found in the Prp19 complex. The Prp19 complex associates with the spliceosome during its assembly and remodeling recruiting additional proteins.
Component of the XAB2 complex, a multimeric protein complex composed of XAB2, PRPF19, AQR, ZNF830, ISY1, and PPIE.
Interacts with CWC22 and EIF4A3 in an RNA-independent manner.
Interacts with RPA1 and RPA2; the PRP19-CDC5L complex is recruited to the sites of DNA repair where it interacts with the replication protein A complex (RPA).
Interacts with SETMAR; required for SETMAR recruitment to site of DNA damage.
Interacts with U2AF2; the interaction is direct and recruits the Prp19 complex to RNA polymerase II C-terminal domain (CTD) and the pre-mRNA.
Interacts with PRPF3.
Interacts with APEX1, DNTT and PSMB4.
Interacts with KNSTRN (By similarity).
Interacts with PSMC5 (PubMed:17349974). Isoform 2 (via N-terminus) interacts with PPIA. Isoform 2 does not interact with CDC5L (PubMed:16352598).
Interacts with KHDC4 (By similarity).
By similarity2 PublicationsGO - Molecular functioni
- identical protein binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 205709, 54 interactors |
ComplexPortali | CPX-5825, PRP19-CDC5L complex |
IntActi | Q99KP6, 9 interactors |
MINTi | Q99KP6 |
STRINGi | 10090.ENSMUSP00000136858 |
Miscellaneous databases
RNActi | Q99KP6, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 73 | U-boxAdd BLAST | 72 | |
Repeati | 219 – 259 | WD 1Add BLAST | 41 | |
Repeati | 262 – 301 | WD 2Add BLAST | 40 | |
Repeati | 304 – 345 | WD 3Add BLAST | 42 | |
Repeati | 348 – 387 | WD 4Add BLAST | 40 | |
Repeati | 390 – 429 | WD 5Add BLAST | 40 | |
Repeati | 433 – 472 | WD 6Add BLAST | 40 | |
Repeati | 473 – 503 | WD 7Add BLAST | 31 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 68 – 223 | May mediate interaction with PSMC51 PublicationAdd BLAST | 156 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Repeat, WD repeatPhylogenomic databases
eggNOGi | KOG0289, Eukaryota |
GeneTreei | ENSGT00940000153662 |
HOGENOMi | CLU_023894_1_1_1 |
InParanoidi | Q99KP6 |
OMAi | QHWAFSD |
OrthoDBi | 1049599at2759 |
PhylomeDBi | Q99KP6 |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR020472, G-protein_beta_WD-40_rep IPR013915, Pre-mRNA_splic_Prp19 IPR038959, Prp19 IPR000772, Ricin_B_lectin IPR003613, Ubox_domain IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR017986, WD40_repeat_dom IPR036322, WD40_repeat_dom_sf IPR013083, Znf_RING/FYVE/PHD |
PANTHERi | PTHR43995, PTHR43995, 1 hit |
Pfami | View protein in Pfam PF08606, Prp19, 1 hit PF04564, U-box, 1 hit PF00400, WD40, 5 hits |
PRINTSi | PR00320, GPROTEINBRPT |
SMARTi | View protein in SMART SM00504, Ubox, 1 hit SM00320, WD40, 7 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS51698, U_BOX, 1 hit PS00678, WD_REPEATS_1, 1 hit PS50082, WD_REPEATS_2, 4 hits PS50294, WD_REPEATS_REGION, 1 hit |
s (3)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSLICSISNE VPEHPCVSPV SNHVYERRLI EKYIAENGTD PINNQPLSEE
60 70 80 90 100
QLIDIKVAHP IRPKPPSATS IPAILKALQD EWDAVMLHSF TLRQQLQTTR
110 120 130 140 150
QELSHALYQH DAACRVIARL TKEVTAAREA LATLKPQAGL IVPQAVPSSQ
160 170 180 190 200
PSVVGAGEPM DLGELVGMTP EIIQKLQDKA TVLTTERKKR GKTVPEELVK
210 220 230 240 250
PEELSKYRQV ASHVGLHSAS IPGILALDLC PSDTNKILTG GADKNVVVFD
260 270 280 290 300
KSTEQILATL KGHTKKVTSV VFHPSQELVF SASPDATIRI WSVPNTSCVQ
310 320 330 340 350
VVRAHESAVT GLSLHATGDY LLSSSDDQYW AFSDIQTGRV LTKVTDETSG
360 370 380 390 400
CSLTCAQFHP DGLIFGTGTM DSQIKIWDLK ERTNVANFPG HSGPITSIAF
410 420 430 440 450
SENGYYLATA ADDSSVKLWD LRKLKNFKTL QLDNNFEVKS LIFDQSGTYL
460 470 480 490 500
ALGGTDVQIY ICKQWTEILH FTEHSGLTTG VAFGHHAKFI ASTGMDRSLK
FYSL
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_012192 | 1 – 85 | Missing in isoform 3. 1 PublicationAdd BLAST | 85 | |
Alternative sequenceiVSP_011945 | 82 | W → WGWPNSPALPQSSQWPTLSQ in isoform 2. 1 Publication1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB222602 mRNA Translation: BAE16340.1 AF251503 mRNA Translation: AAK49039.1 AF386760 Genomic DNA Translation: AAM21468.1 AK047693 mRNA Translation: BAC33127.1 AK076998 mRNA Translation: BAC36557.1 AK088769 mRNA Translation: BAC40560.1 AK164681 mRNA Translation: BAE37873.1 BC004070 mRNA Translation: AAH04070.1 |
CCDSi | CCDS57135.1 [Q99KP6-2] CCDS79687.1 [Q99KP6-1] |
RefSeqi | NP_001240772.1, NM_001253843.1 [Q99KP6-2] NP_001240773.1, NM_001253844.1 [Q99KP6-3] NP_598890.1, NM_134129.4 [Q99KP6-1] |
Genome annotation databases
Ensembli | ENSMUST00000025642; ENSMUSP00000025642; ENSMUSG00000024735 [Q99KP6-1] ENSMUST00000179297; ENSMUSP00000136858; ENSMUSG00000024735 [Q99KP6-2] |
GeneIDi | 28000 |
KEGGi | mmu:28000 |
UCSCi | uc008grf.2, mouse [Q99KP6-1] uc008grg.2, mouse [Q99KP6-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB222602 mRNA Translation: BAE16340.1 AF251503 mRNA Translation: AAK49039.1 AF386760 Genomic DNA Translation: AAM21468.1 AK047693 mRNA Translation: BAC33127.1 AK076998 mRNA Translation: BAC36557.1 AK088769 mRNA Translation: BAC40560.1 AK164681 mRNA Translation: BAE37873.1 BC004070 mRNA Translation: AAH04070.1 |
CCDSi | CCDS57135.1 [Q99KP6-2] CCDS79687.1 [Q99KP6-1] |
RefSeqi | NP_001240772.1, NM_001253843.1 [Q99KP6-2] NP_001240773.1, NM_001253844.1 [Q99KP6-3] NP_598890.1, NM_134129.4 [Q99KP6-1] |
3D structure databases
SMRi | Q99KP6 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 205709, 54 interactors |
ComplexPortali | CPX-5825, PRP19-CDC5L complex |
IntActi | Q99KP6, 9 interactors |
MINTi | Q99KP6 |
STRINGi | 10090.ENSMUSP00000136858 |
PTM databases
iPTMneti | Q99KP6 |
PhosphoSitePlusi | Q99KP6 |
SwissPalmi | Q99KP6 |
2D gel databases
REPRODUCTION-2DPAGEi | Q99KP6 |
Proteomic databases
EPDi | Q99KP6 |
jPOSTi | Q99KP6 |
PaxDbi | Q99KP6 |
PeptideAtlasi | Q99KP6 |
PRIDEi | Q99KP6 |
Protocols and materials databases
Antibodypediai | 3247, 302 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000025642; ENSMUSP00000025642; ENSMUSG00000024735 [Q99KP6-1] ENSMUST00000179297; ENSMUSP00000136858; ENSMUSG00000024735 [Q99KP6-2] |
GeneIDi | 28000 |
KEGGi | mmu:28000 |
UCSCi | uc008grf.2, mouse [Q99KP6-1] uc008grg.2, mouse [Q99KP6-2] |
Organism-specific databases
CTDi | 27339 |
MGIi | MGI:106247, Prpf19 |
Phylogenomic databases
eggNOGi | KOG0289, Eukaryota |
GeneTreei | ENSGT00940000153662 |
HOGENOMi | CLU_023894_1_1_1 |
InParanoidi | Q99KP6 |
OMAi | QHWAFSD |
OrthoDBi | 1049599at2759 |
PhylomeDBi | Q99KP6 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Reactomei | R-MMU-6781823, Formation of TC-NER Pre-Incision Complex R-MMU-6782135, Dual incision in TC-NER R-MMU-6782210, Gap-filling DNA repair synthesis and ligation in TC-NER R-MMU-72163, mRNA Splicing - Major Pathway |
Miscellaneous databases
BioGRID-ORCSi | 28000, 11 hits in 20 CRISPR screens |
ChiTaRSi | Prpf19, mouse |
PROi | PR:Q99KP6 |
RNActi | Q99KP6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000024735, Expressed in supraoptic nucleus and 294 other tissues |
Genevisiblei | Q99KP6, MM |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR020472, G-protein_beta_WD-40_rep IPR013915, Pre-mRNA_splic_Prp19 IPR038959, Prp19 IPR000772, Ricin_B_lectin IPR003613, Ubox_domain IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR017986, WD40_repeat_dom IPR036322, WD40_repeat_dom_sf IPR013083, Znf_RING/FYVE/PHD |
PANTHERi | PTHR43995, PTHR43995, 1 hit |
Pfami | View protein in Pfam PF08606, Prp19, 1 hit PF04564, U-box, 1 hit PF00400, WD40, 5 hits |
PRINTSi | PR00320, GPROTEINBRPT |
SMARTi | View protein in SMART SM00504, Ubox, 1 hit SM00320, WD40, 7 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS51698, U_BOX, 1 hit PS00678, WD_REPEATS_1, 1 hit PS50082, WD_REPEATS_2, 4 hits PS50294, WD_REPEATS_REGION, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PRP19_MOUSE | |
Accessioni | Q99KP6Primary (citable) accession number: Q99KP6 Secondary accession number(s): Q3TP64 Q8BVQ4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 23, 2004 |
Last sequence update: | June 1, 2001 | |
Last modified: | February 10, 2021 | |
This is version 173 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families