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UniProtKB - Q99KP6 (PRP19_MOUSE)

Entry version 176 (29 Sep 2021)
Sequence version 1 (01 Jun 2001)
Previous versions | rss
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Protein

Pre-mRNA-processing factor 19

Gene

Prpf19

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Ubiquitin-protein ligase which is a core component of several complexes mainly involved in pre-mRNA splicing and DNA repair. Required for pre-mRNA splicing as component of the spliceosome. Core component of the PRP19C/Prp19 complex/NTC/Nineteen complex which is part of the spliceosome and participates in its assembly, its remodeling and is required for its activity. During assembly of the spliceosome, mediates 'Lys-63'-linked polyubiquitination of the U4 spliceosomal protein PRPF3. Ubiquitination of PRPF3 allows its recognition by the U5 component PRPF8 and stabilizes the U4/U5/U6 tri-snRNP spliceosomal complex. Recruited to RNA polymerase II C-terminal domain (CTD) and the pre-mRNA, it may also couple the transcriptional and spliceosomal machineries. The XAB2 complex, which contains PRPF19, is also involved in pre-mRNA splicing, transcription and transcription-coupled repair. Beside its role in pre-mRNA splicing PRPF19, as part of the PRP19-CDC5L complex, plays a role in the DNA damage response/DDR. It is recruited to the sites of DNA damage by the RPA complex where PRPF19 directly ubiquitinates RPA1 and RPA2. 'Lys-63'-linked polyubiquitination of the RPA complex allows the recruitment of the ATR-ATRIP complex and the activation of ATR, a master regulator of the DNA damage response. May also play a role in DNA double-strand break (DSB) repair by recruiting the repair factor SETMAR to altered DNA. As part of the PSO4 complex may also be involved in the DNA interstrand cross-links/ICLs repair process. In addition, may also mediate 'Lys-48'-linked polyubiquitination of substrates and play a role in proteasomal degradation (PubMed:17349974).

May play a role in the biogenesis of lipid droplets (PubMed:17118936).

May play a role in neural differentiation possibly through its function as part of the spliceosome (By similarity).

By similarity2 Publications

Forced expression leads to suppression of neuronal differentiation, and on the contrary to stimulation of astroglial cell differentiation in retinoic acid-primed P19 cells (PubMed:16352598).

1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

  • S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.By similarity EC:2.3.2.27

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.By similarity
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionTransferase
Biological processDNA damage, DNA repair, mRNA processing, mRNA splicing, Ubl conjugation pathway

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...Reactomei		R-MMU-6781823, Formation of TC-NER Pre-Incision Complex
R-MMU-6782135, Dual incision in TC-NER
R-MMU-6782210, Gap-filling DNA repair synthesis and ligation in TC-NER
R-MMU-72163, mRNA Splicing - Major Pathway

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...UniPathwayi		UPA00143

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Pre-mRNA-processing factor 19Curated (EC:2.3.2.27By similarity)
Alternative name(s):
Nuclear matrix protein 2001 Publication
PRP19/PSO4 homolog
RING-type E3 ubiquitin transferase PRP19Curated
Senescence evasion factor1 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:Prpf19Imported
Synonyms:Prp19, Snev1 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMus musculus (Mouse)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri10090 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000589 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 19

Organism-specific databases

Mouse genome database (MGD) from Mouse Genome Informatics (MGI)

More...MGIi		MGI:106247, Prpf19

Eukaryotic Pathogen, Vector and Host Database Resources

More...VEuPathDBi		HostDB:ENSMUSG00000024735

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Lipid droplet, Nucleus, Spliceosome

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM / Processing</a> section indicates that the initiator methionine is cleaved from the mature protein.<p><a href='/help/init_met' target='_top'>More...</a></p>Initiator methionineiRemovedBy similarity
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000511462 – 504Pre-mRNA-processing factor 19Add BLAST503

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei2N-acetylserineBy similarity1
Modified residuei122N6-acetyllysineBy similarity1
Modified residuei179N6-acetyllysineCombined sources1
Modified residuei244N6-acetyllysineCombined sources1
Modified residuei261N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

Encyclopedia of Proteome Dynamics

More...EPDi		Q99KP6

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		Q99KP6

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		Q99KP6

PeptideAtlas

More...PeptideAtlasi		Q99KP6

PRoteomics IDEntifications database

More...PRIDEi		Q99KP6

ProteomicsDB: a multi-organism proteome resource

More...ProteomicsDBi		291890 [Q99KP6-1]
291891 [Q99KP6-2]
291892 [Q99KP6-3]

2D gel databases

REPRODUCTION-2DPAGE

More...REPRODUCTION-2DPAGEi		Q99KP6

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		Q99KP6

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...PhosphoSitePlusi		Q99KP6

SwissPalm database of S-palmitoylation events

More...SwissPalmi		Q99KP6

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Expressed in white and brown adipose tissues, brain and to a lower extent in liver, kidney, muscle, lung and spleen (at protein level).1 Publication

<p>This subsection of the 'Expression' section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

Up-regulated in differentiating adipocytes (PubMed:17118936). Isoform 2 maximal expression level during the neural differentiation of P19 cells treated with retinoic acid (RA) is estimated to be 2.5-fold of the expression level of the untreated cells and it is detected 1-2 days after RA treatment and it decreases steeply thereafter to the basal level. Isoform 2 expression level increases steeply after 1 day of RA treatment and is estimated to be 4.2-fold of the original level at 0 hours (PubMed:16352598).2 Publications

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...Bgeei		ENSMUSG00000024735, Expressed in supraoptic nucleus and 302 other tissues

Genevisible search portal to normalized and curated expression data from Genevestigator

More...Genevisiblei		Q99KP6, MM

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homotetramer.

Component of activated, catalytic and post-catalytic spliceosomes.

Component of the Prp19 complex/PRP19C/Nineteen complex/NTC and related complexes described as PRP19-CDC5L splicing complex and PSO4 complex. A homotetramer of PRPF19, CDC5L, PLRG1 and BCAS2 constitute the core of those complexes. The interaction with CDC5L, PLRG1 and BCAS2 is direct within this core complex. At least three less stably associated proteins CTNNBL1, CWC15 and HSPA8 are found in the Prp19 complex. The Prp19 complex associates with the spliceosome during its assembly and remodeling recruiting additional proteins.

Component of the XAB2 complex, a multimeric protein complex composed of XAB2, PRPF19, AQR, ZNF830, ISY1, and PPIE.

Interacts with CWC22 and EIF4A3 in an RNA-independent manner.

Interacts with RPA1 and RPA2; the PRP19-CDC5L complex is recruited to the sites of DNA repair where it interacts with the replication protein A complex (RPA).

Interacts with SETMAR; required for SETMAR recruitment to site of DNA damage.

Interacts with U2AF2; the interaction is direct and recruits the Prp19 complex to RNA polymerase II C-terminal domain (CTD) and the pre-mRNA.

Interacts with PRPF3.

Interacts with APEX1, DNTT and PSMB4.

Interacts with KNSTRN (By similarity).

Interacts with PSMC5 (PubMed:17349974). Isoform 2 (via N-terminus) interacts with PPIA. Isoform 2 does not interact with CDC5L (PubMed:16352598).

Interacts with KHDC4 (By similarity).

By similarity2 Publications

GO - Molecular functioni

Complete GO annotation on QuickGO ...

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		205709, 56 interactors

ComplexPortal: manually curated resource of macromolecular complexes

More...ComplexPortali		CPX-5825, PRP19-CDC5L complex

Protein interaction database and analysis system

More...IntActi		Q99KP6, 8 interactors

Molecular INTeraction database

More...MINTi		Q99KP6

STRING: functional protein association networks

More...STRINGi		10090.ENSMUSP00000136858

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...RNActi		Q99KP6, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		Q99KP6

Database of comparative protein structure models

More...ModBasei		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini2 – 73U-boxAdd BLAST72
<p>This subsection of the 'Family and Domains' section indicates the positions and types of repeated sequence motifs or repeated domains within the protein.<p><a href='/help/repeat' target='_top'>More...</a></p>Repeati219 – 259WD 1Add BLAST41
Repeati262 – 301WD 2Add BLAST40
Repeati304 – 345WD 3Add BLAST42
Repeati348 – 387WD 4Add BLAST40
Repeati390 – 429WD 5Add BLAST40
Repeati433 – 472WD 6Add BLAST40
Repeati473 – 503WD 7Add BLAST31

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni68 – 223May mediate interaction with PSMC51 PublicationAdd BLAST156

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The 7 WD repeats are necessary and sufficient to support interaction with the RPA complex.By similarity

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the WD repeat PRP19 family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		KOG0289, Eukaryota

Ensembl GeneTree

More...GeneTreei		ENSGT00940000153662

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_023894_1_1_1

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		Q99KP6

Identification of Orthologs from Complete Genome Data

More...OMAi		QHWAFSD

Database of Orthologous Groups

More...OrthoDBi		1049599at2759

Database for complete collections of gene phylogenies

More...PhylomeDBi		Q99KP6

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		2.130.10.10, 1 hit
3.30.40.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR020472, G-protein_beta_WD-40_rep
IPR013915, Pre-mRNA_splic_Prp19
IPR038959, Prp19
IPR000772, Ricin_B_lectin
IPR003613, Ubox_domain
IPR015943, WD40/YVTN_repeat-like_dom_sf
IPR001680, WD40_repeat
IPR019775, WD40_repeat_CS
IPR036322, WD40_repeat_dom_sf
IPR013083, Znf_RING/FYVE/PHD

The PANTHER Classification System

More...PANTHERi		PTHR43995, PTHR43995, 1 hit

Pfam protein domain database

More...Pfami		View protein in Pfam
PF08606, Prp19, 1 hit
PF04564, U-box, 1 hit
PF00400, WD40, 5 hits

Protein Motif fingerprint database; a protein domain database

More...PRINTSi		PR00320, GPROTEINBRPT

Simple Modular Architecture Research Tool; a protein domain database

More...SMARTi		View protein in SMART
SM00504, Ubox, 1 hit
SM00320, WD40, 7 hits

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF50978, SSF50978, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS51698, U_BOX, 1 hit
PS00678, WD_REPEATS_1, 1 hit
PS50082, WD_REPEATS_2, 4 hits
PS50294, WD_REPEATS_REGION, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (3)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q99KP6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSLICSISNE VPEHPCVSPV SNHVYERRLI EKYIAENGTD PINNQPLSEE 
        60         70         80         90        100
QLIDIKVAHP IRPKPPSATS IPAILKALQD EWDAVMLHSF TLRQQLQTTR 
       110        120        130        140        150
QELSHALYQH DAACRVIARL TKEVTAAREA LATLKPQAGL IVPQAVPSSQ 
       160        170        180        190        200
PSVVGAGEPM DLGELVGMTP EIIQKLQDKA TVLTTERKKR GKTVPEELVK 
       210        220        230        240        250
PEELSKYRQV ASHVGLHSAS IPGILALDLC PSDTNKILTG GADKNVVVFD 
       260        270        280        290        300
KSTEQILATL KGHTKKVTSV VFHPSQELVF SASPDATIRI WSVPNTSCVQ 
       310        320        330        340        350
VVRAHESAVT GLSLHATGDY LLSSSDDQYW AFSDIQTGRV LTKVTDETSG 
       360        370        380        390        400
CSLTCAQFHP DGLIFGTGTM DSQIKIWDLK ERTNVANFPG HSGPITSIAF 
       410        420        430        440        450
SENGYYLATA ADDSSVKLWD LRKLKNFKTL QLDNNFEVKS LIFDQSGTYL 
       460        470        480        490        500
ALGGTDVQIY ICKQWTEILH FTEHSGLTTG VAFGHHAKFI ASTGMDRSLK 

FYSL
Length:504
Mass (Da):55,239
Last modified:June 1, 2001 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iB2918188A0651024
GO
Isoform 2 (identifier: Q99KP6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     82-82: W → WGWPNSPALPQSSQWPTLSQ

Show »
Length:523
Mass (Da):57,302
Checksum:i4867008E9F3D969E
GO
Isoform 3 (identifier: Q99KP6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-85: Missing.

Show »
Length:419
Mass (Da):45,789
Checksum:i56AD02B0FBFB1B48
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_0121921 – 85Missing in isoform 3. 1 PublicationAdd BLAST85
Alternative sequenceiVSP_01194582W → WGWPNSPALPQSSQWPTLSQ in isoform 2. 1 Publication1 Publication1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
AB222602 mRNA Translation: BAE16340.1
AF251503 mRNA Translation: AAK49039.1
AF386760 Genomic DNA Translation: AAM21468.1
AK047693 mRNA Translation: BAC33127.1
AK076998 mRNA Translation: BAC36557.1
AK088769 mRNA Translation: BAC40560.1
AK164681 mRNA Translation: BAE37873.1
BC004070 mRNA Translation: AAH04070.1

The Consensus CDS (CCDS) project

More...CCDSi		CCDS57135.1 [Q99KP6-2]
CCDS79687.1 [Q99KP6-1]

NCBI Reference Sequences

More...RefSeqi		NP_001240772.1, NM_001253843.1 [Q99KP6-2]
NP_001240773.1, NM_001253844.1 [Q99KP6-3]
NP_598890.1, NM_134129.4 [Q99KP6-1]

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...Ensembli		ENSMUST00000025642; ENSMUSP00000025642; ENSMUSG00000024735 [Q99KP6-1]
ENSMUST00000179297; ENSMUSP00000136858; ENSMUSG00000024735 [Q99KP6-2]

Database of genes from NCBI RefSeq genomes

More...GeneIDi		28000

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		mmu:28000

UCSC genome browser

More...UCSCi		uc008grf.2, mouse [Q99KP6-1]
uc008grg.2, mouse [Q99KP6-2]

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB222602 mRNA Translation: BAE16340.1
AF251503 mRNA Translation: AAK49039.1
AF386760 Genomic DNA Translation: AAM21468.1
AK047693 mRNA Translation: BAC33127.1
AK076998 mRNA Translation: BAC36557.1
AK088769 mRNA Translation: BAC40560.1
AK164681 mRNA Translation: BAE37873.1
BC004070 mRNA Translation: AAH04070.1
CCDSiCCDS57135.1 [Q99KP6-2]
CCDS79687.1 [Q99KP6-1]
RefSeqiNP_001240772.1, NM_001253843.1 [Q99KP6-2]
NP_001240773.1, NM_001253844.1 [Q99KP6-3]
NP_598890.1, NM_134129.4 [Q99KP6-1]

3D structure databases

SMRiQ99KP6
ModBaseiSearch...

Protein-protein interaction databases

BioGRIDi205709, 56 interactors
ComplexPortaliCPX-5825, PRP19-CDC5L complex
IntActiQ99KP6, 8 interactors
MINTiQ99KP6
STRINGi10090.ENSMUSP00000136858

PTM databases

iPTMnetiQ99KP6
PhosphoSitePlusiQ99KP6
SwissPalmiQ99KP6

2D gel databases

REPRODUCTION-2DPAGEiQ99KP6

Proteomic databases

EPDiQ99KP6
jPOSTiQ99KP6
PaxDbiQ99KP6
PeptideAtlasiQ99KP6
PRIDEiQ99KP6
ProteomicsDBi291890 [Q99KP6-1]
291891 [Q99KP6-2]
291892 [Q99KP6-3]

Protocols and materials databases

Antibodypedia a portal for validated antibodies

More...Antibodypediai		3247, 313 antibodies

The DNASU plasmid repository

More...DNASUi		28000

Genome annotation databases

EnsembliENSMUST00000025642; ENSMUSP00000025642; ENSMUSG00000024735 [Q99KP6-1]
ENSMUST00000179297; ENSMUSP00000136858; ENSMUSG00000024735 [Q99KP6-2]
GeneIDi28000
KEGGimmu:28000
UCSCiuc008grf.2, mouse [Q99KP6-1]
uc008grg.2, mouse [Q99KP6-2]

Organism-specific databases

Comparative Toxicogenomics Database

More...CTDi		27339
MGIiMGI:106247, Prpf19
VEuPathDBiHostDB:ENSMUSG00000024735

Phylogenomic databases

eggNOGiKOG0289, Eukaryota
GeneTreeiENSGT00940000153662
HOGENOMiCLU_023894_1_1_1
InParanoidiQ99KP6
OMAiQHWAFSD
OrthoDBi1049599at2759
PhylomeDBiQ99KP6

Enzyme and pathway databases

UniPathwayiUPA00143
ReactomeiR-MMU-6781823, Formation of TC-NER Pre-Incision Complex
R-MMU-6782135, Dual incision in TC-NER
R-MMU-6782210, Gap-filling DNA repair synthesis and ligation in TC-NER
R-MMU-72163, mRNA Splicing - Major Pathway

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...BioGRID-ORCSi		28000, 36 hits in 101 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...ChiTaRSi		Prpf19, mouse

Protein Ontology

More...PROi		PR:Q99KP6
RNActiQ99KP6, protein

The Stanford Online Universal Resource for Clones and ESTs

More...SOURCEi		Search...

Gene expression databases

BgeeiENSMUSG00000024735, Expressed in supraoptic nucleus and 302 other tissues
GenevisibleiQ99KP6, MM

Family and domain databases

Gene3Di2.130.10.10, 1 hit
3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR020472, G-protein_beta_WD-40_rep
IPR013915, Pre-mRNA_splic_Prp19
IPR038959, Prp19
IPR000772, Ricin_B_lectin
IPR003613, Ubox_domain
IPR015943, WD40/YVTN_repeat-like_dom_sf
IPR001680, WD40_repeat
IPR019775, WD40_repeat_CS
IPR036322, WD40_repeat_dom_sf
IPR013083, Znf_RING/FYVE/PHD
PANTHERiPTHR43995, PTHR43995, 1 hit
PfamiView protein in Pfam
PF08606, Prp19, 1 hit
PF04564, U-box, 1 hit
PF00400, WD40, 5 hits
PRINTSiPR00320, GPROTEINBRPT
SMARTiView protein in SMART
SM00504, Ubox, 1 hit
SM00320, WD40, 7 hits
SUPFAMiSSF50978, SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS51698, U_BOX, 1 hit
PS00678, WD_REPEATS_1, 1 hit
PS50082, WD_REPEATS_2, 4 hits
PS50294, WD_REPEATS_REGION, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiPRP19_MOUSE
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q99KP6
Secondary accession number(s): Q3TP64
, Q4ADG5, Q8BKZ5, Q8BVQ4
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: June 1, 2001
Last modified: September 29, 2021
This is version 176 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families
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