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Protein

Probable cytosolic iron-sulfur protein assembly protein CIAO1

Gene

Ciao1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins (By similarity). As a CIA complex component, interacts specifically with CIAO2A or CIAO2B and MMS19 to assist different branches of iron-sulfur protein assembly, depending of its interactors. The complex CIAO1:CIAO2B:MMS19 binds to and facilitates the assembly of most cytosolic-nuclear Fe/S proteins. CIAO1:CIAO2A specifically matures ACO1 and stabilizes IREB2 (By similarity). Seems to specifically modulate the transactivation activity of WT1. As part of the mitotic spindle-associated MMXD complex it may play a role in chromosome segregation (By similarity).UniRule annotationBy similarity

GO - Molecular functioni

  • DNA-binding transcription factor activity Source: MGI

GO - Biological processi

Keywordsi

Biological processChromosome partition

Names & Taxonomyi

Protein namesi
Recommended name:
Probable cytosolic iron-sulfur protein assembly protein CIAO1UniRule annotation
Alternative name(s):
WD repeat-containing protein 39UniRule annotation
Gene namesi
Name:Ciao1UniRule annotation
Synonyms:Wdr39
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1346998 Ciao1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002811061 – 339Probable cytosolic iron-sulfur protein assembly protein CIAO1Add BLAST339

Proteomic databases

EPDiQ99KN2
MaxQBiQ99KN2
PaxDbiQ99KN2
PeptideAtlasiQ99KN2
PRIDEiQ99KN2

PTM databases

iPTMnetiQ99KN2
PhosphoSitePlusiQ99KN2

Expressioni

Gene expression databases

BgeeiENSMUSG00000003662 Expressed in 301 organ(s), highest expression level in primary oocyte
ExpressionAtlasiQ99KN2 baseline and differential
GenevisibleiQ99KN2 MM

Interactioni

Subunit structurei

Component of the CIA complex. Interacts with CIAO2A and forms a complex with CIAO2B and MMS19; the interactions with CIAO2A and CIAO2B are mutually exclusive (PubMed:23891004) (By similarity). Interacts with CHD1L, ERCC2, IREB2 and POLD1 (By similarity). Component of the MMXD complex, which includes CIAO1, ERCC2, CIAO2B, MMS19 and SLC25A5. Interacts with WT1 (By similarity). Component of the CIA complex. Interacts with CIAO2A and forms a complex with CIAO2B and MMS19; the interactions with CIAO2A and CIAO2B are mutually exclusive. Interacts with CHD1L, ERCC2, IREB2 and POLD1. Component of the MMXD complex, which includes CIAO1, ERCC2, CIAO2B, MMS19 and SLC25A5. Interacts with WT1. Interacts with CIAO3 (By similarity).UniRule annotationBy similarity1 Publication

Protein-protein interaction databases

BioGridi204931, 1 interactor
IntActiQ99KN2, 4 interactors
MINTiQ99KN2
STRINGi10090.ENSMUSP00000003759

Structurei

3D structure databases

ProteinModelPortaliQ99KN2
SMRiQ99KN2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati14 – 53WD 1Add BLAST40
Repeati59 – 98WD 2Add BLAST40
Repeati103 – 142WD 3Add BLAST40
Repeati148 – 187WD 4Add BLAST40
Repeati192 – 231WD 5Add BLAST40
Repeati250 – 289WD 6Add BLAST40
Repeati301 – 339WD 7Add BLAST39

Sequence similaritiesi

Belongs to the WD repeat CIA1 family.UniRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0645 Eukaryota
ENOG410XRMA LUCA
GeneTreeiENSGT00890000139406
HOGENOMiHOG000208901
HOVERGENiHBG056532
InParanoidiQ99KN2
OMAiEFECAAV
OrthoDBiEOG091G0AAM
PhylomeDBiQ99KN2
TreeFamiTF318181

Family and domain databases

Gene3Di2.130.10.10, 1 hit
HAMAPiMF_03037 ciao1, 1 hit
InterProiView protein in InterPro
IPR028608 CIAO1/Cia1
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PANTHERiPTHR19920 PTHR19920, 1 hit
PfamiView protein in Pfam
PF00400 WD40, 6 hits
SMARTiView protein in SMART
SM00320 WD40, 7 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 1 hit
PS50082 WD_REPEATS_2, 6 hits
PS50294 WD_REPEATS_REGION, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

Q99KN2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKDSLVLQSR VPAHPDSRCW FLAWNPSGTL LASCGGDRKI RIWGTEGDSW
60 70 80 90 100
ICKSVLSEGH QRTVRKVAWS PCGNYLASAS FDATTCIWKK NQDDFECVTT
110 120 130 140 150
LEGHENEVKS VAWAPSGNLL ATCSRDKSVW VWEVDEEDEY ECVSVLSSHT
160 170 180 190 200
QDVKHVVWHP SQELLASASY DDTVKLYQEE GDDWVCCATL EGHESTVWSI
210 220 230 240 250
AFDPSGQRLA SCSDDRTVRI WRQYLPGNEQ GVACSGSDPS WKCICTLSGF
260 270 280 290 300
HTRTIYDVAW CQLTGALATA CGDDAIRVFE EDPGSDPQQP TFSLTAHLRQ
310 320 330
AHSQDVNCVA WNPKEPGLLA SCSDDGEVAF WEYHQPAGL
Length:339
Mass (Da):37,633
Last modified:June 1, 2001 - v1
Checksum:i302A9FCD8E110FC0
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G3UYR5G3UYR5_MOUSE
Probable cytosolic iron-sulfur prot...
Ciao1
233Annotation score:
G3UYN9G3UYN9_MOUSE
Probable cytosolic iron-sulfur prot...
Ciao1
176Annotation score:
G3UYS4G3UYS4_MOUSE
Probable cytosolic iron-sulfur prot...
Ciao1
51Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti65R → M in BAB22008 (PubMed:16141072).Curated1
Sequence conflicti213S → N in BAB22008 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002314 mRNA Translation: BAB22008.1
AK028376 mRNA Translation: BAC25915.1
AL845368 Genomic DNA No translation available.
BC004089 mRNA Translation: AAH04089.1
CCDSiCCDS16696.1
RefSeqiNP_079572.2, NM_025296.4
UniGeneiMm.45179

Genome annotation databases

EnsembliENSMUST00000003759; ENSMUSP00000003759; ENSMUSG00000003662
GeneIDi26371
KEGGimmu:26371
UCSCiuc008mfb.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002314 mRNA Translation: BAB22008.1
AK028376 mRNA Translation: BAC25915.1
AL845368 Genomic DNA No translation available.
BC004089 mRNA Translation: AAH04089.1
CCDSiCCDS16696.1
RefSeqiNP_079572.2, NM_025296.4
UniGeneiMm.45179

3D structure databases

ProteinModelPortaliQ99KN2
SMRiQ99KN2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204931, 1 interactor
IntActiQ99KN2, 4 interactors
MINTiQ99KN2
STRINGi10090.ENSMUSP00000003759

PTM databases

iPTMnetiQ99KN2
PhosphoSitePlusiQ99KN2

Proteomic databases

EPDiQ99KN2
MaxQBiQ99KN2
PaxDbiQ99KN2
PeptideAtlasiQ99KN2
PRIDEiQ99KN2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000003759; ENSMUSP00000003759; ENSMUSG00000003662
GeneIDi26371
KEGGimmu:26371
UCSCiuc008mfb.2 mouse

Organism-specific databases

CTDi9391
MGIiMGI:1346998 Ciao1

Phylogenomic databases

eggNOGiKOG0645 Eukaryota
ENOG410XRMA LUCA
GeneTreeiENSGT00890000139406
HOGENOMiHOG000208901
HOVERGENiHBG056532
InParanoidiQ99KN2
OMAiEFECAAV
OrthoDBiEOG091G0AAM
PhylomeDBiQ99KN2
TreeFamiTF318181

Miscellaneous databases

ChiTaRSiCiao1 mouse
PROiPR:Q99KN2
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000003662 Expressed in 301 organ(s), highest expression level in primary oocyte
ExpressionAtlasiQ99KN2 baseline and differential
GenevisibleiQ99KN2 MM

Family and domain databases

Gene3Di2.130.10.10, 1 hit
HAMAPiMF_03037 ciao1, 1 hit
InterProiView protein in InterPro
IPR028608 CIAO1/Cia1
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PANTHERiPTHR19920 PTHR19920, 1 hit
PfamiView protein in Pfam
PF00400 WD40, 6 hits
SMARTiView protein in SMART
SM00320 WD40, 7 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 1 hit
PS50082 WD_REPEATS_2, 6 hits
PS50294 WD_REPEATS_REGION, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCIAO1_MOUSE
AccessioniPrimary (citable) accession number: Q99KN2
Secondary accession number(s): Q9DCZ7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: June 1, 2001
Last modified: November 7, 2018
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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