Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Transmembrane emp24 domain-containing protein 9

Gene

Tmed9

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Appears to be involved in vesicular protein trafficking, mainly in the early secretory pathway. In COPI vesicle-mediated retrograde transport involved in the coatomer recruitment to membranes of the early secretory pathway. Increases coatomer-dependent activity of ARFGAP2. Thought to play a crucial role in the specific retention of p24 complexes in cis-Golgi membranes; specifically contributes to the coupled localization of TMED2 and TMED10 in the cis-Golgi network. May be involved in organization of intracellular membranes, such as of the ER-Golgi intermediate compartment and the Golgi apparatus. Involved in ER localization of PTPN2 (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-6807878 COPI-mediated anterograde transport
R-MMU-6811434 COPI-dependent Golgi-to-ER retrograde traffic

Names & Taxonomyi

Protein namesi
Recommended name:
Transmembrane emp24 domain-containing protein 9
Alternative name(s):
Glycoprotein 25L2
p24 family protein alpha-2
Short name:
p24alpha2
Gene namesi
Name:Tmed9
Synonyms:Gp25l2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1914761 Tmed9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini38 – 202LumenalSequence analysisAdd BLAST165
Transmembranei203 – 222HelicalSequence analysisAdd BLAST20
Topological domaini223 – 235CytoplasmicSequence analysisAdd BLAST13

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 37By similarityAdd BLAST37
ChainiPRO_000001039738 – 235Transmembrane emp24 domain-containing protein 9Add BLAST198

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi125N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei160N6-acetyllysineCombined sources1

Post-translational modificationi

N-linked glycosylated containing high mannose.By similarity

Keywords - PTMi

Acetylation, Glycoprotein

Proteomic databases

EPDiQ99KF1
MaxQBiQ99KF1
PaxDbiQ99KF1
PeptideAtlasiQ99KF1
PRIDEiQ99KF1
TopDownProteomicsiQ99KF1

PTM databases

iPTMnetiQ99KF1
PhosphoSitePlusiQ99KF1

Expressioni

Gene expression databases

BgeeiENSMUSG00000058569 Expressed in 275 organ(s), highest expression level in prostate gland
ExpressionAtlasiQ99KF1 baseline and differential
GenevisibleiQ99KF1 MM

Interactioni

Subunit structurei

Monomer and homodimer in endoplasmic reticulum. Predominantly monomeric and to lesser extent homodimeric in endoplasmic reticulum-Golgi intermediate compartment and cis-Golgi network. Probably oligomerizes with other members of the EMP24/GP25L family such as TMED2, TMED7 and TMED10. Interacts with TMED5. Interacts (via C-terminus) with COPG1; the interaction involves dimeric TMED9. Interacts with PTPN2 and SPAST. Interacts with STX17; the interaction is direct (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi212239, 1 interactor
IntActiQ99KF1, 2 interactors
STRINGi10090.ENSMUSP00000105531

Structurei

3D structure databases

SMRiQ99KF1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini47 – 145GOLDPROSITE-ProRule annotationAdd BLAST99

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni121 – 160Required for interaction with STX17By similarityAdd BLAST40

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili154 – 184Sequence analysisAdd BLAST31

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi228 – 235COPI vesicle coat-bindingSequence analysis8
Motifi228 – 229COPII vesicle coat-bindingSequence analysis2

Sequence similaritiesi

Belongs to the EMP24/GP25L family.Curated

Keywords - Domaini

Coiled coil, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1690 Eukaryota
ENOG410XPUW LUCA
GeneTreeiENSGT00430000030848
HOGENOMiHOG000160228
HOVERGENiHBG105357
InParanoidiQ99KF1
KOiK20346
OMAiVLWWSIV
OrthoDBiEOG091G0JDW
PhylomeDBiQ99KF1
TreeFamiTF314123

Family and domain databases

InterProiView protein in InterPro
IPR009038 GOLD_dom
IPR015720 TMP21-related
PANTHERiPTHR22811 PTHR22811, 1 hit
PfamiView protein in Pfam
PF01105 EMP24_GP25L, 1 hit
SMARTiView protein in SMART
SM01190 EMP24_GP25L, 1 hit
PROSITEiView protein in PROSITE
PS50866 GOLD, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.iShow all

Q99KF1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAAVRGVRVV GSSPGLLLGR GMRAFLLLLW LAARGSALYF HIGETEKKCF
60 70 80 90 100
IEEIPDETMV IGNYRTQLYD KQREEYQPAT PGLGMFVEVK DPEDKVILAR
110 120 130 140 150
QYGSEGRFTF TSHTPGEHQI CLHSNSTKFS LFAGGMLRVH LDIQVGEHAN
160 170 180 190 200
DYAEIAAKDK LSELQLRVRQ LVEQVEQIQK EQNYQRWREE RFRQTSESTN
210 220 230
QRVLWWSILQ TLILVAIGVW QMRHLKSFFE AKKLV
Length:235
Mass (Da):27,127
Last modified:June 16, 2009 - v2
Checksum:i7B035FB91F1EDEEB
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A286YDS5A0A286YDS5_MOUSE
Transmembrane emp24 domain-containi...
Tmed9
209Annotation score:

Sequence cautioni

The sequence AAH04691 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti208I → V in AAH04691 (PubMed:15489334).Curated1
Sequence conflicti215V → L in AAH04691 (PubMed:15489334).Curated1
Sequence conflicti220W → C in AAH04691 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK010259 mRNA Translation: BAB26802.2
BC004691 mRNA Translation: AAH04691.1 Different initiation.
CCDSiCCDS49277.1
RefSeqiNP_080487.2, NM_026211.3
UniGeneiMm.45233

Genome annotation databases

EnsembliENSMUST00000109905; ENSMUSP00000105531; ENSMUSG00000058569
GeneIDi67511
KEGGimmu:67511
UCSCiuc007qrr.1 mouse

Similar proteinsi

Entry informationi

Entry nameiTMED9_MOUSE
AccessioniPrimary (citable) accession number: Q99KF1
Secondary accession number(s): Q9CX02
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: June 16, 2009
Last modified: September 12, 2018
This is version 125 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again