UniProtKB - Q99KE1 (MAOM_MOUSE)
Protein
NAD-dependent malic enzyme, mitochondrial
Gene
Me2
Organism
Mus musculus (Mouse)
Status
Functioni
Miscellaneous
This isoenzyme can also use NADP+ but is more effective with NAD+.By similarity
Catalytic activityi
Cofactori
Mg2+By similarity, Mn2+By similarityNote: Divalent metal cations. Prefers magnesium or manganese.By similarity
Activity regulationi
Subject to allosteric activation by fumarate.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 112 | Proton donorBy similarity | 1 | |
Binding sitei | 165 | NADBy similarity | 1 | |
Active sitei | 183 | Proton acceptorBy similarity | 1 | |
Metal bindingi | 255 | Divalent metal cationBy similarity | 1 | |
Metal bindingi | 256 | Divalent metal cationBy similarity | 1 | |
Metal bindingi | 279 | Divalent metal cationBy similarity | 1 | |
Binding sitei | 279 | NADBy similarity | 1 | |
Sitei | 279 | Important for activityBy similarity | 1 | |
Binding sitei | 421 | NADBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 311 – 328 | NADBy similarityAdd BLAST | 18 |
GO - Molecular functioni
- malate dehydrogenase (decarboxylating) (NAD+) activity Source: UniProtKB-EC
- malate dehydrogenase (decarboxylating) (NADP+) activity Source: GO_Central
- malic enzyme activity Source: MGI
- metal ion binding Source: UniProtKB-KW
- NAD binding Source: InterPro
- oxaloacetate decarboxylase activity Source: UniProtKB-EC
GO - Biological processi
- malate metabolic process Source: GO_Central
- pyruvate metabolic process Source: GO_Central
- regulation of NADP metabolic process Source: MGI
Keywordsi
Molecular function | Allosteric enzyme, Oxidoreductase |
Ligand | Metal-binding, NAD |
Enzyme and pathway databases
BRENDAi | 1.1.1.38, 3474 |
Reactomei | R-MMU-71403, Citric acid cycle (TCA cycle) |
Names & Taxonomyi
Protein namesi | Recommended name: NAD-dependent malic enzyme, mitochondrial (EC:1.1.1.38)Short name: NAD-ME Alternative name(s): Malic enzyme 2 |
Gene namesi | Name:Me2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2147351, Me2 |
Subcellular locationi
Mitochondrion
- Mitochondrion matrix By similarity
Mitochondrion
- mitochondrial matrix Source: UniProtKB-SubCell
- mitochondrion Source: MGI
Other locations
- intracellular membrane-bounded organelle Source: MGI
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 18 | MitochondrionBy similarityAdd BLAST | 18 | |
ChainiPRO_0000018538 | 19 – 589 | NAD-dependent malic enzyme, mitochondrialAdd BLAST | 571 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 156 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 224 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 240 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 272 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 346 | N6-acetyllysineBy similarity | 1 |
Keywords - PTMi
AcetylationProteomic databases
EPDi | Q99KE1 |
jPOSTi | Q99KE1 |
MaxQBi | Q99KE1 |
PaxDbi | Q99KE1 |
PeptideAtlasi | Q99KE1 |
PRIDEi | Q99KE1 |
2D gel databases
REPRODUCTION-2DPAGEi | Q99KE1 |
PTM databases
PhosphoSitePlusi | Q99KE1 |
SwissPalmi | Q99KE1 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000024556, Expressed in jejunum and 288 other tissues |
Genevisiblei | Q99KE1, MM |
Interactioni
Subunit structurei
Homotetramer.
By similarityProtein-protein interaction databases
BioGRIDi | 223188, 1 interactor |
IntActi | Q99KE1, 1 interactor |
MINTi | Q99KE1 |
STRINGi | 10090.ENSMUSP00000025439 |
Miscellaneous databases
RNActi | Q99KE1, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the malic enzymes family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG1257, Eukaryota |
GeneTreei | ENSGT00950000183134 |
HOGENOMi | CLU_011405_5_0_1 |
InParanoidi | Q99KE1 |
OMAi | GVNGMPI |
OrthoDBi | 435571at2759 |
PhylomeDBi | Q99KE1 |
TreeFami | TF300537 |
Family and domain databases
Gene3Di | 3.40.50.10380, 1 hit |
InterProi | View protein in InterPro IPR015884, Malic_enzyme_CS IPR012301, Malic_N_dom IPR037062, Malic_N_dom_sf IPR012302, Malic_NAD-bd IPR001891, Malic_OxRdtase IPR036291, NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF00390, malic, 1 hit PF03949, Malic_M, 1 hit |
PIRSFi | PIRSF000106, ME, 1 hit |
PRINTSi | PR00072, MALOXRDTASE |
SMARTi | View protein in SMART SM01274, malic, 1 hit SM00919, Malic_M, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00331, MALIC_ENZYMES, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q99KE1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFSRLRAVTT PCTLTCRRVH LKEKGKPLML NPRTNKGMAF TLQERQMLGL
60 70 80 90 100
QGLLPPKIET QDIQALRFHR NLKKMTSPLE KYIYIMGIQE RNEKLFYRIL
110 120 130 140 150
QDDIESLMPI VYTPTVGLAC CQYGHIFRRP KGLFISISDR GHVRSIVDNW
160 170 180 190 200
PENHVKAVVV TDGERILGLG DLGVYGMGIP VGKLCLYTAC AGIQPEKCLP
210 220 230 240 250
VCIDVGTDNM ALLKDPFYMG LYQKRDRSQL YDDLMDEFMK AITDRYGRNT
260 270 280 290 300
LIQFEDFGNH NAFRFLRKYQ QKYCTFNDDI QGTAAVALSG LLAAQRVINK
310 320 330 340 350
PVSEHKILFL GAGEAALGIA NLIVLSMVES GLSEEEAQRK IWMFDKSGLL
360 370 380 390 400
VKGRTASIDS NQEPYAHAAP ESIPATFEDA VNKLKPSVII GVAGAGPLFT
410 420 430 440 450
HGVIKAMASI NERPIIFALS NPTAQAECTA EDAYTLTEGR CLFASGSPFE
460 470 480 490 500
PVKLQDGRVF TPGQGNNAYI FPGVALAVIL CEARHISDTV FLEAAKALTT
510 520 530 540 550
QLTDAELAQG RLYPSLANIQ EVSANIAIKL AEYLYANKMA FRYPEPEDKA
560 570 580
RYVRERIWRS NYVSLLPDVY DWPESSLTPP QITEEKLPH
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK042289 mRNA Translation: BAC31216.1 AK050933 mRNA Translation: BAC34467.1 AK050980 mRNA Translation: BAC34483.1 AK156403 mRNA Translation: BAE33701.1 AK171157 mRNA Translation: BAE42281.1 BC004709 mRNA Translation: AAH04709.1 |
CCDSi | CCDS29339.1 |
RefSeqi | NP_663469.1, NM_145494.2 |
Genome annotation databases
Ensembli | ENSMUST00000025439; ENSMUSP00000025439; ENSMUSG00000024556 |
GeneIDi | 107029 |
KEGGi | mmu:107029 |
UCSCi | uc008fox.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK042289 mRNA Translation: BAC31216.1 AK050933 mRNA Translation: BAC34467.1 AK050980 mRNA Translation: BAC34483.1 AK156403 mRNA Translation: BAE33701.1 AK171157 mRNA Translation: BAE42281.1 BC004709 mRNA Translation: AAH04709.1 |
CCDSi | CCDS29339.1 |
RefSeqi | NP_663469.1, NM_145494.2 |
3D structure databases
SMRi | Q99KE1 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 223188, 1 interactor |
IntActi | Q99KE1, 1 interactor |
MINTi | Q99KE1 |
STRINGi | 10090.ENSMUSP00000025439 |
PTM databases
PhosphoSitePlusi | Q99KE1 |
SwissPalmi | Q99KE1 |
2D gel databases
REPRODUCTION-2DPAGEi | Q99KE1 |
Proteomic databases
EPDi | Q99KE1 |
jPOSTi | Q99KE1 |
MaxQBi | Q99KE1 |
PaxDbi | Q99KE1 |
PeptideAtlasi | Q99KE1 |
PRIDEi | Q99KE1 |
Protocols and materials databases
Antibodypediai | 1636, 200 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000025439; ENSMUSP00000025439; ENSMUSG00000024556 |
GeneIDi | 107029 |
KEGGi | mmu:107029 |
UCSCi | uc008fox.1, mouse |
Organism-specific databases
CTDi | 4200 |
MGIi | MGI:2147351, Me2 |
Phylogenomic databases
eggNOGi | KOG1257, Eukaryota |
GeneTreei | ENSGT00950000183134 |
HOGENOMi | CLU_011405_5_0_1 |
InParanoidi | Q99KE1 |
OMAi | GVNGMPI |
OrthoDBi | 435571at2759 |
PhylomeDBi | Q99KE1 |
TreeFami | TF300537 |
Enzyme and pathway databases
BRENDAi | 1.1.1.38, 3474 |
Reactomei | R-MMU-71403, Citric acid cycle (TCA cycle) |
Miscellaneous databases
BioGRID-ORCSi | 107029, 5 hits in 17 CRISPR screens |
ChiTaRSi | Me2, mouse |
PROi | PR:Q99KE1 |
RNActi | Q99KE1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000024556, Expressed in jejunum and 288 other tissues |
Genevisiblei | Q99KE1, MM |
Family and domain databases
Gene3Di | 3.40.50.10380, 1 hit |
InterProi | View protein in InterPro IPR015884, Malic_enzyme_CS IPR012301, Malic_N_dom IPR037062, Malic_N_dom_sf IPR012302, Malic_NAD-bd IPR001891, Malic_OxRdtase IPR036291, NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF00390, malic, 1 hit PF03949, Malic_M, 1 hit |
PIRSFi | PIRSF000106, ME, 1 hit |
PRINTSi | PR00072, MALOXRDTASE |
SMARTi | View protein in SMART SM01274, malic, 1 hit SM00919, Malic_M, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00331, MALIC_ENZYMES, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MAOM_MOUSE | |
Accessioni | Q99KE1Primary (citable) accession number: Q99KE1 Secondary accession number(s): Q3TBM8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 9, 2004 |
Last sequence update: | June 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 150 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families