UniProtKB - Q99K85 (SERC_MOUSE)
Protein
Phosphoserine aminotransferase
Gene
Psat1
Organism
Mus musculus (Mouse)
Status
Functioni
Catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine.By similarity
Catalytic activityi
- EC:2.6.1.52
- 2-oxoglutarate + 4-(phosphooxy)-L-threonine = (R)-3-hydroxy-2-oxo-4-phosphooxybutanoate + L-glutamateEC:2.6.1.52
Cofactori
pyridoxal 5'-phosphateBy similarityNote: Binds 1 pyridoxal phosphate per subunit.By similarity
: L-serine biosynthesis Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes L-serine from 3-phospho-D-glycerate.Proteins known to be involved in the 3 steps of the subpathway in this organism are:
- D-3-phosphoglycerate dehydrogenase (Phgdh)
- Phosphoserine aminotransferase (Psat1), Phosphoserine aminotransferase (Psat1), Phosphoserine aminotransferase (Psat1), Phosphoserine aminotransferase (Psat1), Phosphoserine aminotransferase (Psat1)
- Phosphoserine phosphatase (Psph)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-serine from 3-phospho-D-glycerate, the pathway L-serine biosynthesis and in Amino-acid biosynthesis.
Pathwayi: pyridoxine 5'-phosphate biosynthesis
This protein is involved in step 3 of the subpathway that synthesizes pyridoxine 5'-phosphate from D-erythrose 4-phosphate.Proteins known to be involved in the 5 steps of the subpathway in this organism are:
- no protein annotated in this organism
- no protein annotated in this organism
- Phosphoserine aminotransferase (Psat1)
- no protein annotated in this organism
- no protein annotated in this organism
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyridoxine 5'-phosphate from D-erythrose 4-phosphate, the pathway pyridoxine 5'-phosphate biosynthesis and in Cofactor biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 45 | L-glutamateBy similarity | 1 | |
Binding sitei | 107 | Pyridoxal phosphateBy similarity | 1 | |
Binding sitei | 156 | Pyridoxal phosphateBy similarity | 1 | |
Binding sitei | 176 | Pyridoxal phosphateBy similarity | 1 | |
Binding sitei | 199 | Pyridoxal phosphateBy similarity | 1 |
GO - Molecular functioni
- O-phospho-L-serine:2-oxoglutarate aminotransferase activity Source: GO_Central
GO - Biological processi
- L-serine biosynthetic process Source: GO_Central
Keywordsi
Molecular function | Aminotransferase, Transferase |
Biological process | Amino-acid biosynthesis, Serine biosynthesis |
Ligand | Pyridoxal phosphate |
Enzyme and pathway databases
Reactomei | R-MMU-977347 Serine biosynthesis |
UniPathwayi | UPA00135;UER00197 UPA00244;UER00311 |
Names & Taxonomyi
Protein namesi | Recommended name: Phosphoserine aminotransferase (EC:2.6.1.52)Short name: PSAT Alternative name(s): Endometrial progesterone-induced protein Short name: EPIP Phosphohydroxythreonine aminotransferase |
Gene namesi | Name:Psat1 Synonyms:Psa, Psat |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2183441 Psat1 |
Subcellular locationi
Cytosol
- cytosol Source: MGI
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000150136 | 1 – 370 | Phosphoserine aminotransferaseAdd BLAST | 370 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 51 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 127 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 200 | N6-(pyridoxal phosphate)lysineBy similarity | 1 | |
Modified residuei | 269 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 318 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 323 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 331 | PhosphoserineBy similarity | 1 | |
Modified residuei | 333 | N6-acetyllysineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q99K85 |
jPOSTi | Q99K85 |
MaxQBi | Q99K85 |
PaxDbi | Q99K85 |
PRIDEi | Q99K85 |
PTM databases
iPTMneti | Q99K85 |
PhosphoSitePlusi | Q99K85 |
SwissPalmi | Q99K85 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000024640 Expressed in 321 organ(s), highest expression level in embryo |
ExpressionAtlasi | Q99K85 baseline and differential |
Genevisiblei | Q99K85 MM |
Interactioni
Subunit structurei
Homodimer.
By similarityProtein-protein interaction databases
BioGridi | 223231, 1 interactor |
IntActi | Q99K85, 3 interactors |
MINTi | Q99K85 |
STRINGi | 10090.ENSMUSP00000025542 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 79 – 80 | Pyridoxal phosphate bindingBy similarity | 2 | |
Regioni | 241 – 242 | Pyridoxal phosphate bindingBy similarity | 2 |
Sequence similaritiesi
Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily.Curated
Phylogenomic databases
eggNOGi | KOG2790 Eukaryota COG1932 LUCA |
GeneTreei | ENSGT00940000153241 |
HOGENOMi | HOG000088965 |
InParanoidi | Q99K85 |
KOi | K00831 |
OMAi | GAQKNMG |
OrthoDBi | 1357311at2759 |
PhylomeDBi | Q99K85 |
TreeFami | TF312975 |
Family and domain databases
CDDi | cd00611 PSAT_like, 1 hit |
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
HAMAPi | MF_00160 SerC_aminotrans_5, 1 hit |
InterProi | View protein in InterPro IPR000192 Aminotrans_V_dom IPR020578 Aminotrans_V_PyrdxlP_BS IPR022278 Pser_aminoTfrase IPR015424 PyrdxlP-dep_Trfase IPR015422 PyrdxlP-dep_Trfase_dom1 IPR015421 PyrdxlP-dep_Trfase_major |
Pfami | View protein in Pfam PF00266 Aminotran_5, 1 hit |
PIRSFi | PIRSF000525 SerC, 1 hit |
SUPFAMi | SSF53383 SSF53383, 1 hit |
TIGRFAMsi | TIGR01364 serC_1, 1 hit |
PROSITEi | View protein in PROSITE PS00595 AA_TRANSFER_CLASS_5, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q99K85-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEATKQVVNF GPGPAKLPHS VLLEIQKQLL DYRGLGISVL EMSHRSSDFA
60 70 80 90 100
KIIGNTENLV RELLAVPNNY KVIFVQGGGS GQFSAVPLNL IGLKAGRSAD
110 120 130 140 150
YVVTGAWSAK AAEEAKKFGT VNIVHPKLGS YTKIPDPSTW NLNPDASYVY
160 170 180 190 200
FCANETVHGV EFDFVPDVKG AVLVCDMSSN FLSRPVDVSK FGVIFAGAQK
210 220 230 240 250
NVGSAGVTVV IVRDDLLGFS LRECPSVLDY KVQAGNNSLY NTPPCFSIYV
260 270 280 290 300
MGMVLEWIKN NGGAAAMEKL SSIKSQMIYE IIDNSQGFYV CPVERQNRSR
310 320 330 340 350
MNIPFRIGNA KGDEALEKRF LDKAVELNMI SLKGHRSVGG IRASLYNAVT
360 370
TEDVEKLAAF MKNFLEMHQL
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ3U6K9 | Q3U6K9_MOUSE | Phosphoserine aminotransferase | Psat1 | 367 | Annotation score: | ||
E9Q6P1 | E9Q6P1_MOUSE | Phosphoserine aminotransferase | Psat1 | 157 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF259674 mRNA Translation: AAK69389.1 BC004827 mRNA Translation: AAH04827.1 |
CCDSi | CCDS29681.1 |
RefSeqi | NP_803155.1, NM_177420.2 |
Genome annotation databases
Ensembli | ENSMUST00000025542; ENSMUSP00000025542; ENSMUSG00000024640 |
GeneIDi | 107272 |
KEGGi | mmu:107272 |
UCSCi | uc008gwp.2 mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF259674 mRNA Translation: AAK69389.1 BC004827 mRNA Translation: AAH04827.1 |
CCDSi | CCDS29681.1 |
RefSeqi | NP_803155.1, NM_177420.2 |
3D structure databases
SMRi | Q99K85 |
ModBasei | Search... |
Protein-protein interaction databases
BioGridi | 223231, 1 interactor |
IntActi | Q99K85, 3 interactors |
MINTi | Q99K85 |
STRINGi | 10090.ENSMUSP00000025542 |
PTM databases
iPTMneti | Q99K85 |
PhosphoSitePlusi | Q99K85 |
SwissPalmi | Q99K85 |
Proteomic databases
EPDi | Q99K85 |
jPOSTi | Q99K85 |
MaxQBi | Q99K85 |
PaxDbi | Q99K85 |
PRIDEi | Q99K85 |
Genome annotation databases
Ensembli | ENSMUST00000025542; ENSMUSP00000025542; ENSMUSG00000024640 |
GeneIDi | 107272 |
KEGGi | mmu:107272 |
UCSCi | uc008gwp.2 mouse |
Organism-specific databases
CTDi | 29968 |
MGIi | MGI:2183441 Psat1 |
Phylogenomic databases
eggNOGi | KOG2790 Eukaryota COG1932 LUCA |
GeneTreei | ENSGT00940000153241 |
HOGENOMi | HOG000088965 |
InParanoidi | Q99K85 |
KOi | K00831 |
OMAi | GAQKNMG |
OrthoDBi | 1357311at2759 |
PhylomeDBi | Q99K85 |
TreeFami | TF312975 |
Enzyme and pathway databases
UniPathwayi | UPA00135;UER00197 UPA00244;UER00311 |
Reactomei | R-MMU-977347 Serine biosynthesis |
Miscellaneous databases
ChiTaRSi | Psat1 mouse |
PROi | PR:Q99K85 |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000024640 Expressed in 321 organ(s), highest expression level in embryo |
ExpressionAtlasi | Q99K85 baseline and differential |
Genevisiblei | Q99K85 MM |
Family and domain databases
CDDi | cd00611 PSAT_like, 1 hit |
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
HAMAPi | MF_00160 SerC_aminotrans_5, 1 hit |
InterProi | View protein in InterPro IPR000192 Aminotrans_V_dom IPR020578 Aminotrans_V_PyrdxlP_BS IPR022278 Pser_aminoTfrase IPR015424 PyrdxlP-dep_Trfase IPR015422 PyrdxlP-dep_Trfase_dom1 IPR015421 PyrdxlP-dep_Trfase_major |
Pfami | View protein in Pfam PF00266 Aminotran_5, 1 hit |
PIRSFi | PIRSF000525 SerC, 1 hit |
SUPFAMi | SSF53383 SSF53383, 1 hit |
TIGRFAMsi | TIGR01364 serC_1, 1 hit |
PROSITEi | View protein in PROSITE PS00595 AA_TRANSFER_CLASS_5, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SERC_MOUSE | |
Accessioni | Q99K85Primary (citable) accession number: Q99K85 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 23, 2002 |
Last sequence update: | June 1, 2001 | |
Last modified: | May 8, 2019 | |
This is version 154 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways