UniProtKB - Q99K48 (NONO_MOUSE)
Non-POU domain-containing octamer-binding protein
Nono
Functioni
GO - Molecular functioni
- chromatin binding Source: BHF-UCL
- identical protein binding Source: MGI
- nucleic acid binding Source: GO_Central
- RNA binding Source: UniProtKB-KW
- transcription regulatory region sequence-specific DNA binding Source: GO_Central
GO - Biological processi
- activation of innate immune response Source: MGI
- circadian rhythm Source: UniProtKB
- DNA recombination Source: UniProtKB-KW
- DNA repair Source: UniProtKB-KW
- innate immune response Source: UniProtKB-KW
- mRNA splicing, via spliceosome Source: GO_Central
- negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway Source: MGI
- negative regulation of transcription, DNA-templated Source: UniProtKB
- regulation of circadian rhythm Source: UniProtKB
- regulation of transcription, DNA-templated Source: GO_Central
Keywordsi
Molecular function | Activator, DNA-binding, Repressor, RNA-binding |
Biological process | Biological rhythms, DNA damage, DNA recombination, DNA repair, Immunity, Innate immunity, mRNA processing, mRNA splicing, Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Non-POU domain-containing octamer-binding proteinShort name: NonO protein |
Gene namesi | Name:Nono |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1855692, Nono |
Subcellular locationi
Nucleus
- Nucleus 2 Publications
- nucleolus By similarity
- Nucleus speckle By similarity
Note: Detected in punctate subnuclear structures often located adjacent to splicing speckles, called paraspeckles.By similarity
Nucleus
- fibrillar center Source: MGI
- nuclear matrix Source: MGI
- nuclear speck Source: UniProtKB-SubCell
- nucleoplasm Source: MGI
- nucleus Source: UniProtKB
- paraspeckles Source: MGI
Other locations
- RNA polymerase II transcription regulator complex Source: BHF-UCL
Keywords - Cellular componenti
NucleusPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000081684 | 1 – 473 | Non-POU domain-containing octamer-binding proteinAdd BLAST | 473 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineCombined sources | 1 | |
Modified residuei | 5 | N6-acetyllysine; alternateCombined sources | 1 | |
Cross-linki | 5 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 11 | N6-acetyllysineCombined sources | 1 | |
Cross-linki | 62 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 98 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 101 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 128 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 149 | PhosphoserineBy similarity | 1 | |
Cross-linki | 192 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 200 | N6-acetyllysine; alternateCombined sources | 1 | |
Cross-linki | 200 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Cross-linki | 245 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 251 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 264 | PhosphoserineBy similarity | 1 | |
Modified residuei | 297 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 373 | N6-acetyllysine; alternateCombined sources | 1 | |
Cross-linki | 373 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 430 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 442 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 452 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 458 | Omega-N-methylarginineBy similarity | 1 | |
Cross-linki | 469 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity |
Keywords - PTMi
Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q99K48 |
jPOSTi | Q99K48 |
PaxDbi | Q99K48 |
PeptideAtlasi | Q99K48 |
PRIDEi | Q99K48 |
TopDownProteomicsi | Q99K48-1 [Q99K48-1] |
PTM databases
iPTMneti | Q99K48 |
PhosphoSitePlusi | Q99K48 |
SwissPalmi | Q99K48 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000031311, Expressed in undifferentiated genital tubercle and 247 other tissues |
ExpressionAtlasi | Q99K48, baseline and differential |
Genevisiblei | Q99K48, MM |
Interactioni
Subunit structurei
Monomer and component of the SFPQ-NONO complex, which is probably a heterotetramer of two 52 kDa (NONO) and two 100 kDa (SFPQ) subunits. NONO is a component of spliceosome and U5.4/6 snRNP complexes (By similarity).
Interacts with CPNE4 (via VWFA domain) (PubMed:12522145). Forms heterodimers with PSPC1; this involves formation of a coiled coil domain by helices from both proteins (PubMed:15140795). Part of complex consisting of SFPQ, NONO and MATR3.
Part of a complex consisting of SFPQ, NONO and NR5A1.
Part of a complex consisting of SFPQ, NONO and TOP1.
Interacts with SPI1 (PubMed:8626664).
Interacts with RNF43 (By similarity).
Interacts with PER1 and PER2 (PubMed:22966205). Part of the HDP-RNP complex composed of at least HEXIM1, PRKDC, XRCC5, XRCC6, paraspeckle proteins (SFPQ, NONO, PSPC1, RBM14, and MATR3) and NEAT1 RNA.
Interacts (via second RRM domain) with WASL; the interaction is direct.
Component of a multiprotein complex with WASL and SFPQ (By similarity).
Interacts with ERCC6 (By similarity).
By similarity4 PublicationsBinary interactionsi
Q99K48
With | #Exp. | IntAct |
---|---|---|
Spi1 [P17433] | 3 | EBI-607499,EBI-607588 |
GO - Molecular functioni
- identical protein binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 207330, 39 interactors |
DIPi | DIP-34308N |
ELMi | Q99K48 |
IntActi | Q99K48, 8 interactors |
MINTi | Q99K48 |
STRINGi | 10090.ENSMUSP00000033673 |
Miscellaneous databases
RNActi | Q99K48, protein |
Structurei
Secondary structure
3D structure databases
BMRBi | Q99K48 |
SMRi | Q99K48 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q99K48 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 76 – 143 | RRM 1PROSITE-ProRule annotationAdd BLAST | 68 | |
Domaini | 150 – 231 | RRM 2PROSITE-ProRule annotationAdd BLAST | 82 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 56 – 375 | DBHSBy similarityAdd BLAST | 320 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 270 – 374 | Sequence analysisAdd BLAST | 105 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 29 – 38 | Poly-Gln | 10 | |
Compositional biasi | 39 – 44 | Poly-Pro | 6 | |
Compositional biasi | 350 – 353 | Poly-Arg | 4 |
Keywords - Domaini
Coiled coil, RepeatPhylogenomic databases
eggNOGi | KOG0115, Eukaryota |
GeneTreei | ENSGT00940000154442 |
HOGENOMi | CLU_027185_2_0_1 |
InParanoidi | Q99K48 |
OMAi | CSDGAYL |
TreeFami | TF315795 |
Family and domain databases
CDDi | cd12588, RRM1_p54nrb, 1 hit |
Gene3Di | 3.30.70.330, 2 hits |
InterProi | View protein in InterPro IPR012975, NOPS IPR012677, Nucleotide-bd_a/b_plait_sf IPR034552, p54nrb_RRM1 IPR035979, RBD_domain_sf IPR000504, RRM_dom |
Pfami | View protein in Pfam PF08075, NOPS, 1 hit PF00076, RRM_1, 2 hits |
SMARTi | View protein in SMART SM00360, RRM, 2 hits |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 2 hits |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MQSNKAFNLE KQNHTPRKHH QHHHQQHHQQ QQQQQQQQPP PPIPANGQQA
60 70 80 90 100
SSQNEGLTID LKNFRKPGEK TFTQRSRLFV GNLPPDITEE EMRKLFEKYG
110 120 130 140 150
KAGEVFIHKD KGFGFIRLET RTLAEIAKVE LDNMPLRGKQ LRVRFACHSA
160 170 180 190 200
SLTVRNLPQY VSNELLEEAF SVFGQVERAV VIVDDRGRPS GKGIVEFSGK
210 220 230 240 250
PAARKALDRC SEGSFLLTTF PRPVTVEPMD QLDDEEGLPE KLVIKNQQFH
260 270 280 290 300
KEREQPPRFA QPGSFEYEYA MRWKALIEME KQQQDQVDRN IKEAREKLEM
310 320 330 340 350
EMEAARHEHQ VMLMRQDLMR RQEELRRMEE LHNQEVQKRK QLELRQEEER
360 370 380 390 400
RRREEEMRRQ QEEMMRRQQE GFKGTFPDAR EQEIRMGQMA MGGAMGINNR
410 420 430 440 450
GAMPPAPVPP GTPAPPGPAT MMPDGTLGLT PPTTERFGQA ATMEGIGAIG
460 470
GTPPAFNRPA PGAEFAPNKR RRY
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketB1AXT0 | B1AXT0_MOUSE | Non-POU domain-containing octamer-b... | Nono | 98 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 76 | S → T in BAB28857 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 117 | R → N in BAB28857 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 127 | A → V in AAB27887 (PubMed:8355702).Curated | 1 | |
Sequence conflicti | 153 | T → K in BAB28857 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 167 | E → G in BAB28857 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 183 | V → E in BAB28857 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 188 | R → W in BAB28857 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 203 – 204 | AR → VL in BAB28857 (PubMed:16141072).Curated | 2 | |
Sequence conflicti | 222 | R → W in AAB27887 (PubMed:8355702).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_013981 | 220 – 473 | Missing in isoform 2. 1 PublicationAdd BLAST | 254 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S64860 mRNA Translation: AAB27887.1 AK004830 mRNA Translation: BAB23598.1 Sequence problems. AK013444 mRNA Translation: BAB28857.1 Sequence problems. AK028338 mRNA Translation: BAC25890.1 BC005465 mRNA Translation: AAH05465.1 BC083074 mRNA Translation: AAH83074.1 |
CCDSi | CCDS30316.1 [Q99K48-1] |
RefSeqi | NP_001239447.1, NM_001252518.1 [Q99K48-1] NP_075633.2, NM_023144.2 [Q99K48-1] |
Genome annotation databases
Ensembli | ENSMUST00000033673; ENSMUSP00000033673; ENSMUSG00000031311 [Q99K48-1] |
GeneIDi | 53610 |
KEGGi | mmu:53610 |
UCSCi | uc009txr.2, mouse [Q99K48-2] uc009txs.2, mouse [Q99K48-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S64860 mRNA Translation: AAB27887.1 AK004830 mRNA Translation: BAB23598.1 Sequence problems. AK013444 mRNA Translation: BAB28857.1 Sequence problems. AK028338 mRNA Translation: BAC25890.1 BC005465 mRNA Translation: AAH05465.1 BC083074 mRNA Translation: AAH83074.1 |
CCDSi | CCDS30316.1 [Q99K48-1] |
RefSeqi | NP_001239447.1, NM_001252518.1 [Q99K48-1] NP_075633.2, NM_023144.2 [Q99K48-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2CPJ | NMR | - | A | 68-153 | [»] | |
2RS8 | NMR | - | A | 68-153 | [»] | |
BMRBi | Q99K48 | |||||
SMRi | Q99K48 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 207330, 39 interactors |
DIPi | DIP-34308N |
ELMi | Q99K48 |
IntActi | Q99K48, 8 interactors |
MINTi | Q99K48 |
STRINGi | 10090.ENSMUSP00000033673 |
PTM databases
iPTMneti | Q99K48 |
PhosphoSitePlusi | Q99K48 |
SwissPalmi | Q99K48 |
Proteomic databases
EPDi | Q99K48 |
jPOSTi | Q99K48 |
PaxDbi | Q99K48 |
PeptideAtlasi | Q99K48 |
PRIDEi | Q99K48 |
TopDownProteomicsi | Q99K48-1 [Q99K48-1] |
Protocols and materials databases
Antibodypediai | 13516, 525 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000033673; ENSMUSP00000033673; ENSMUSG00000031311 [Q99K48-1] |
GeneIDi | 53610 |
KEGGi | mmu:53610 |
UCSCi | uc009txr.2, mouse [Q99K48-2] uc009txs.2, mouse [Q99K48-1] |
Organism-specific databases
CTDi | 4841 |
MGIi | MGI:1855692, Nono |
Phylogenomic databases
eggNOGi | KOG0115, Eukaryota |
GeneTreei | ENSGT00940000154442 |
HOGENOMi | CLU_027185_2_0_1 |
InParanoidi | Q99K48 |
OMAi | CSDGAYL |
TreeFami | TF315795 |
Miscellaneous databases
BioGRID-ORCSi | 53610, 5 hits in 17 CRISPR screens |
ChiTaRSi | Nono, mouse |
EvolutionaryTracei | Q99K48 |
PROi | PR:Q99K48 |
RNActi | Q99K48, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000031311, Expressed in undifferentiated genital tubercle and 247 other tissues |
ExpressionAtlasi | Q99K48, baseline and differential |
Genevisiblei | Q99K48, MM |
Family and domain databases
CDDi | cd12588, RRM1_p54nrb, 1 hit |
Gene3Di | 3.30.70.330, 2 hits |
InterProi | View protein in InterPro IPR012975, NOPS IPR012677, Nucleotide-bd_a/b_plait_sf IPR034552, p54nrb_RRM1 IPR035979, RBD_domain_sf IPR000504, RRM_dom |
Pfami | View protein in Pfam PF08075, NOPS, 1 hit PF00076, RRM_1, 2 hits |
SMARTi | View protein in SMART SM00360, RRM, 2 hits |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NONO_MOUSE | |
Accessioni | Q99K48Primary (citable) accession number: Q99K48 Secondary accession number(s): Q63887, Q9CYQ4, Q9DBP2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2005 |
Last sequence update: | July 5, 2005 | |
Last modified: | February 10, 2021 | |
This is version 176 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references