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Protein

Non-POU domain-containing octamer-binding protein

Gene

Nono

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA- and RNA binding protein, involved in several nuclear processes. Binds the conventional octamer sequence in double-stranded DNA. Also binds single-stranded DNA and RNA at a site independent of the duplex site. Involved in pre-mRNA splicing, probably as a heterodimer with SFPQ. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. Together with PSPC1, required for the formation of nuclear paraspeckles. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1. The SFPQ-NONO heteromer may be involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. In vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. NONO is involved in transcriptional regulation. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional activity. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. Important for the functional organization of GABAergic synapses. Plays a specific and important role in the regulation of synaptic RNAs and GPHN/gephyrin scaffold structure, through the regulation of GABRA2 transcript (PubMed:26571461).Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway.By similarity4 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding, Repressor, RNA-binding
Biological processBiological rhythms, DNA damage, DNA recombination, DNA repair, Immunity, Innate immunity, mRNA processing, mRNA splicing, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Non-POU domain-containing octamer-binding protein
Short name:
NonO protein
Gene namesi
Name:Nono
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1855692 Nono

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mutant mice display flattened nose and a smaller cerebellum. Behaviorally, mice show impaired spatial memory, as well as a marked anxiety phenotype and increased risk aversion.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000816841 – 473Non-POU domain-containing octamer-binding proteinAdd BLAST473

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei5N6-acetyllysine; alternateCombined sources1
Cross-linki5Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei11N6-acetyllysineCombined sources1
Cross-linki62Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki98Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki101Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki128Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei149PhosphoserineBy similarity1
Cross-linki192Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei200N6-acetyllysine; alternateCombined sources1
Cross-linki200Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Cross-linki245Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki251Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei264PhosphoserineBy similarity1
Modified residuei297N6-acetyllysineCombined sources1
Modified residuei373N6-acetyllysine; alternateCombined sources1
Cross-linki373Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei430PhosphothreonineBy similarity1
Modified residuei442PhosphothreonineBy similarity1
Modified residuei452PhosphothreonineCombined sources1
Modified residuei458Omega-N-methylarginineBy similarity1
Cross-linki469Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ99K48
PaxDbiQ99K48
PeptideAtlasiQ99K48
PRIDEiQ99K48
TopDownProteomicsiQ99K48-1 [Q99K48-1]

PTM databases

iPTMnetiQ99K48
PhosphoSitePlusiQ99K48
SwissPalmiQ99K48

Expressioni

Tissue specificityi

Expressed in liver and suprachiasmatic nuclei, hippocampus and neocortex (at protein level). Expression is strongest in neurons in CA1 and CA3 pyramidal regions and granule cells of the dentate gyrus. Detected in testis and kidney.3 Publications

Gene expression databases

BgeeiENSMUSG00000031311 Expressed in 227 organ(s), highest expression level in thymus
ExpressionAtlasiQ99K48 baseline and differential
GenevisibleiQ99K48 MM

Interactioni

Subunit structurei

Interacts with CPNE4 (via VWFA domain) (PubMed:12522145). Monomer and component of the SFPQ-NONO complex, which is probably a heterotetramer of two 52 kDa (NONO) and two 100 kDa (SFPQ) subunits. NONO is a component of spliceosome and U5.4/6 snRNP complexes. Forms heterodimers with PSPC1; this involves formation of a coiled coil domain by helices from both proteins. Interacts with PSPC1 and SNRPA/U1A. Part of complex consisting of SFPQ, NONO and MATR3. Part of a complex consisting of SFPQ, NONO and NR5A1. Part of a complex consisting of SFPQ, NONO and TOP1. Interacts with SPI1. Interacts with RNF43. Interacts with PER1 and PER2. Part of the HDP-RNP complex composed of at least HEXIM1, PRKDC, XRCC5, XRCC6, paraspeckle proteins (SFPQ, NONO, PSPC1, RBM14, and MATR3) and NEAT1 RNA.By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Spi1P174333EBI-607499,EBI-607588

GO - Molecular functioni

Protein-protein interaction databases

BioGridi207330, 19 interactors
DIPiDIP-34308N
ELMiQ99K48
IntActiQ99K48, 6 interactors
MINTiQ99K48
STRINGi10090.ENSMUSP00000033673

Structurei

Secondary structure

1473
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ99K48
SMRiQ99K48
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ99K48

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini76 – 143RRM 1PROSITE-ProRule annotationAdd BLAST68
Domaini150 – 231RRM 2PROSITE-ProRule annotationAdd BLAST82

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni56 – 375DBHSBy similarityAdd BLAST320

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili270 – 374Sequence analysisAdd BLAST105

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi29 – 38Poly-Gln10
Compositional biasi39 – 44Poly-Pro6
Compositional biasi350 – 353Poly-Arg4

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG0115 Eukaryota
ENOG410XQA0 LUCA
GeneTreeiENSGT00390000005004
HOVERGENiHBG009801
InParanoidiQ99K48
KOiK13214
OMAiNQQYHKE
OrthoDBiEOG091G09T7
TreeFamiTF315795

Family and domain databases

CDDicd12588 RRM1_p54nrb, 1 hit
Gene3Di3.30.70.330, 2 hits
InterProiView protein in InterPro
IPR012975 NOPS
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR034552 p54nrb_RRM1
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
PfamiView protein in Pfam
PF08075 NOPS, 1 hit
PF00076 RRM_1, 2 hits
SMARTiView protein in SMART
SM00360 RRM, 2 hits
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 2 hits

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.iShow all

Isoform 1 (identifier: Q99K48-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MQSNKAFNLE KQNHTPRKHH QHHHQQHHQQ QQQQQQQQPP PPIPANGQQA
60 70 80 90 100
SSQNEGLTID LKNFRKPGEK TFTQRSRLFV GNLPPDITEE EMRKLFEKYG
110 120 130 140 150
KAGEVFIHKD KGFGFIRLET RTLAEIAKVE LDNMPLRGKQ LRVRFACHSA
160 170 180 190 200
SLTVRNLPQY VSNELLEEAF SVFGQVERAV VIVDDRGRPS GKGIVEFSGK
210 220 230 240 250
PAARKALDRC SEGSFLLTTF PRPVTVEPMD QLDDEEGLPE KLVIKNQQFH
260 270 280 290 300
KEREQPPRFA QPGSFEYEYA MRWKALIEME KQQQDQVDRN IKEAREKLEM
310 320 330 340 350
EMEAARHEHQ VMLMRQDLMR RQEELRRMEE LHNQEVQKRK QLELRQEEER
360 370 380 390 400
RRREEEMRRQ QEEMMRRQQE GFKGTFPDAR EQEIRMGQMA MGGAMGINNR
410 420 430 440 450
GAMPPAPVPP GTPAPPGPAT MMPDGTLGLT PPTTERFGQA ATMEGIGAIG
460 470
GTPPAFNRPA PGAEFAPNKR RRY
Length:473
Mass (Da):54,541
Last modified:July 5, 2005 - v3
Checksum:iF65E6AE25D471A38
GO
Isoform 2 (identifier: Q99K48-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     220-473: Missing.

Note: No experimental confirmation available.
Show »
Length:219
Mass (Da):24,969
Checksum:iCFF17042BD1E721E
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B1AXT0B1AXT0_MOUSE
Non-POU domain-containing octamer-b...
Nono
98Annotation score:

Sequence cautioni

The sequence BAB23598 differs from that shown. Reason: Erroneous termination at position 258. Translated as Arg.Curated
The sequence BAB28857 differs from that shown. Reason: Frameshift at position 187.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti76S → T in BAB28857 (PubMed:16141072).Curated1
Sequence conflicti117R → N in BAB28857 (PubMed:16141072).Curated1
Sequence conflicti127A → V in AAB27887 (PubMed:8355702).Curated1
Sequence conflicti153T → K in BAB28857 (PubMed:16141072).Curated1
Sequence conflicti167E → G in BAB28857 (PubMed:16141072).Curated1
Sequence conflicti183V → E in BAB28857 (PubMed:16141072).Curated1
Sequence conflicti188R → W in BAB28857 (PubMed:16141072).Curated1
Sequence conflicti203 – 204AR → VL in BAB28857 (PubMed:16141072).Curated2
Sequence conflicti222R → W in AAB27887 (PubMed:8355702).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_013981220 – 473Missing in isoform 2. 1 PublicationAdd BLAST254

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S64860 mRNA Translation: AAB27887.1
AK004830 mRNA Translation: BAB23598.1 Sequence problems.
AK013444 mRNA Translation: BAB28857.1 Sequence problems.
AK028338 mRNA Translation: BAC25890.1
BC005465 mRNA Translation: AAH05465.1
BC083074 mRNA Translation: AAH83074.1
CCDSiCCDS30316.1 [Q99K48-1]
RefSeqiNP_001239447.1, NM_001252518.1 [Q99K48-1]
NP_075633.2, NM_023144.2 [Q99K48-1]
UniGeneiMm.280069
Mm.475350

Genome annotation databases

EnsembliENSMUST00000033673; ENSMUSP00000033673; ENSMUSG00000031311 [Q99K48-1]
GeneIDi53610
KEGGimmu:53610
UCSCiuc009txr.2 mouse [Q99K48-2]
uc009txs.2 mouse [Q99K48-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S64860 mRNA Translation: AAB27887.1
AK004830 mRNA Translation: BAB23598.1 Sequence problems.
AK013444 mRNA Translation: BAB28857.1 Sequence problems.
AK028338 mRNA Translation: BAC25890.1
BC005465 mRNA Translation: AAH05465.1
BC083074 mRNA Translation: AAH83074.1
CCDSiCCDS30316.1 [Q99K48-1]
RefSeqiNP_001239447.1, NM_001252518.1 [Q99K48-1]
NP_075633.2, NM_023144.2 [Q99K48-1]
UniGeneiMm.280069
Mm.475350

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CPJNMR-A68-153[»]
2RS8NMR-A68-153[»]
ProteinModelPortaliQ99K48
SMRiQ99K48
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207330, 19 interactors
DIPiDIP-34308N
ELMiQ99K48
IntActiQ99K48, 6 interactors
MINTiQ99K48
STRINGi10090.ENSMUSP00000033673

PTM databases

iPTMnetiQ99K48
PhosphoSitePlusiQ99K48
SwissPalmiQ99K48

Proteomic databases

EPDiQ99K48
PaxDbiQ99K48
PeptideAtlasiQ99K48
PRIDEiQ99K48
TopDownProteomicsiQ99K48-1 [Q99K48-1]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033673; ENSMUSP00000033673; ENSMUSG00000031311 [Q99K48-1]
GeneIDi53610
KEGGimmu:53610
UCSCiuc009txr.2 mouse [Q99K48-2]
uc009txs.2 mouse [Q99K48-1]

Organism-specific databases

CTDi4841
MGIiMGI:1855692 Nono

Phylogenomic databases

eggNOGiKOG0115 Eukaryota
ENOG410XQA0 LUCA
GeneTreeiENSGT00390000005004
HOVERGENiHBG009801
InParanoidiQ99K48
KOiK13214
OMAiNQQYHKE
OrthoDBiEOG091G09T7
TreeFamiTF315795

Miscellaneous databases

ChiTaRSiNono mouse
EvolutionaryTraceiQ99K48
PROiPR:Q99K48
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031311 Expressed in 227 organ(s), highest expression level in thymus
ExpressionAtlasiQ99K48 baseline and differential
GenevisibleiQ99K48 MM

Family and domain databases

CDDicd12588 RRM1_p54nrb, 1 hit
Gene3Di3.30.70.330, 2 hits
InterProiView protein in InterPro
IPR012975 NOPS
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR034552 p54nrb_RRM1
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
PfamiView protein in Pfam
PF08075 NOPS, 1 hit
PF00076 RRM_1, 2 hits
SMARTiView protein in SMART
SM00360 RRM, 2 hits
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiNONO_MOUSE
AccessioniPrimary (citable) accession number: Q99K48
Secondary accession number(s): Q63887, Q9CYQ4, Q9DBP2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: July 5, 2005
Last modified: September 12, 2018
This is version 159 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health

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