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Protein

Actin-related protein 3

Gene

Actr3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

ATP-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility. Seems to contact the pointed end of the daughter actin filament. In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs). Plays a role in ciliogenesis.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActin-binding
Biological processCilium biogenesis/degradation
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-2029482 Regulation of actin dynamics for phagocytic cup formation
R-MMU-3928662 EPHB-mediated forward signaling
R-MMU-5663213 RHO GTPases Activate WASPs and WAVEs
R-MMU-8856828 Clathrin-mediated endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Actin-related protein 3
Alternative name(s):
Actin-like protein 3
Gene namesi
Name:Actr3
Synonyms:Arp3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1921367 Actr3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000890802 – 418Actin-related protein 3Add BLAST417

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei240N6-acetyllysineBy similarity1
Modified residuei244N6-acetyllysineCombined sources1
Modified residuei251N6-acetyllysineBy similarity1
Modified residuei254N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ99JY9
MaxQBiQ99JY9
PaxDbiQ99JY9
PRIDEiQ99JY9

PTM databases

iPTMnetiQ99JY9
PhosphoSitePlusiQ99JY9
SwissPalmiQ99JY9

Expressioni

Gene expression databases

BgeeiENSMUSG00000026341 Expressed in 44 organ(s), highest expression level in spleen
CleanExiMM_ACTR3
ExpressionAtlasiQ99JY9 baseline and differential
GenevisibleiQ99JY9 MM

Interactioni

Subunit structurei

Component of the Arp2/3 complex composed of ACTR2/ARP2, ACTR3/ARP3, ARPC1B/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and ARPC5/p16-ARC. Interacts with WHDC1. Interacts weakly with MEFV.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
CttnQ605985EBI-773994,EBI-397955

GO - Molecular functioni

Protein-protein interaction databases

BioGridi216504, 6 interactors
IntActiQ99JY9, 15 interactors
MINTiQ99JY9
STRINGi10090.ENSMUSP00000027579

Structurei

3D structure databases

ProteinModelPortaliQ99JY9
SMRiQ99JY9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the actin family. ARP3 subfamily.Curated

Phylogenomic databases

eggNOGiKOG0678 Eukaryota
COG5277 LUCA
GeneTreeiENSGT00550000074695
HOGENOMiHOG000233339
HOVERGENiHBG003771
InParanoidiQ99JY9
KOiK18584
OMAiAIKHIPI
OrthoDBiEOG091G0BPD
PhylomeDBiQ99JY9
TreeFamiTF300644

Family and domain databases

InterProiView protein in InterPro
IPR004000 Actin
IPR020902 Actin/actin-like_CS
IPR015623 Arp3
PANTHERiPTHR11937 PTHR11937, 1 hit
PTHR11937:SF175 PTHR11937:SF175, 1 hit
PfamiView protein in Pfam
PF00022 Actin, 1 hit
SMARTiView protein in SMART
SM00268 ACTIN, 1 hit
PROSITEiView protein in PROSITE
PS01132 ACTINS_ACT_LIKE, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 6 potential isoforms that are computationally mapped.Show allAlign All

Q99JY9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAGRLPACVV DCGTGYTKLG YAGNTEPQFI IPSCIAIKES AKVGDQAQRR
60 70 80 90 100
VMKGVDDLDF FIGDEAIEKP TYATKWPIRH GIVEDWDLME RFMEQVIFKY
110 120 130 140 150
LRAEPEDHYF LLTEPPLNTP ENREYTAEIM FESFNVPGLY IAVQAVLALA
160 170 180 190 200
ASWTSRQVGE RTLTGTVIDS GDGVTHVIPV AEGYVIGSCI KHIPIAGRDI
210 220 230 240 250
TYFIQQLLRD REVGIPPEQS LETAKAVKER YSYVCPDLVK EFNKYDTDGS
260 270 280 290 300
KWIKQYTGVN AISKKEFSID VGYERFLGPE IFFHPEFANP DFTQPISEVV
310 320 330 340 350
DEVIQNCPID VRRPLYKNIV LSGGSTMFRD FGRRLQRDLK RTVDARLKLS
360 370 380 390 400
EELSGGRLKP KPIDVQVITH HMQRYAVWFG GSMLASTPEF YQVCHTKKDY
410
EEIGPSICRH NPVFGVMS
Length:418
Mass (Da):47,357
Last modified:January 23, 2007 - v3
Checksum:i806FED7A08ABA455
GO

Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A087WRA1A0A087WRA1_MOUSE
Actin-related protein 3
Actr3
216Annotation score:
A0A087WQ14A0A087WQ14_MOUSE
Actin-related protein 3
Actr3
161Annotation score:
A0A087WP86A0A087WP86_MOUSE
Actin-related protein 3
Actr3
155Annotation score:
A0A087WS98A0A087WS98_MOUSE
Actin-related protein 3
Actr3
129Annotation score:
A0A087WQ83A0A087WQ83_MOUSE
Actin-related protein 3
Actr3
70Annotation score:
A0A087WPR6A0A087WPR6_MOUSE
Actin-related protein 3
Actr3
41Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti176H → P in BAB23368 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004554 mRNA Translation: BAB23368.1
AK083343 mRNA Translation: BAC38876.1
BC005557 mRNA Translation: AAH05557.1
BC080806 mRNA Translation: AAH80806.1
CCDSiCCDS15242.1
RefSeqiNP_001192314.1, NM_001205385.1
NP_001192315.1, NM_001205386.1
NP_076224.1, NM_023735.2
UniGeneiMm.183102
Mm.471990

Genome annotation databases

EnsembliENSMUST00000027579; ENSMUSP00000027579; ENSMUSG00000026341
ENSMUST00000178474; ENSMUSP00000137503; ENSMUSG00000026341
GeneIDi74117
KEGGimmu:74117
UCSCiuc007cke.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004554 mRNA Translation: BAB23368.1
AK083343 mRNA Translation: BAC38876.1
BC005557 mRNA Translation: AAH05557.1
BC080806 mRNA Translation: AAH80806.1
CCDSiCCDS15242.1
RefSeqiNP_001192314.1, NM_001205385.1
NP_001192315.1, NM_001205386.1
NP_076224.1, NM_023735.2
UniGeneiMm.183102
Mm.471990

3D structure databases

ProteinModelPortaliQ99JY9
SMRiQ99JY9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi216504, 6 interactors
IntActiQ99JY9, 15 interactors
MINTiQ99JY9
STRINGi10090.ENSMUSP00000027579

PTM databases

iPTMnetiQ99JY9
PhosphoSitePlusiQ99JY9
SwissPalmiQ99JY9

Proteomic databases

EPDiQ99JY9
MaxQBiQ99JY9
PaxDbiQ99JY9
PRIDEiQ99JY9

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027579; ENSMUSP00000027579; ENSMUSG00000026341
ENSMUST00000178474; ENSMUSP00000137503; ENSMUSG00000026341
GeneIDi74117
KEGGimmu:74117
UCSCiuc007cke.2 mouse

Organism-specific databases

CTDi10096
MGIiMGI:1921367 Actr3

Phylogenomic databases

eggNOGiKOG0678 Eukaryota
COG5277 LUCA
GeneTreeiENSGT00550000074695
HOGENOMiHOG000233339
HOVERGENiHBG003771
InParanoidiQ99JY9
KOiK18584
OMAiAIKHIPI
OrthoDBiEOG091G0BPD
PhylomeDBiQ99JY9
TreeFamiTF300644

Enzyme and pathway databases

ReactomeiR-MMU-2029482 Regulation of actin dynamics for phagocytic cup formation
R-MMU-3928662 EPHB-mediated forward signaling
R-MMU-5663213 RHO GTPases Activate WASPs and WAVEs
R-MMU-8856828 Clathrin-mediated endocytosis

Miscellaneous databases

ChiTaRSiActr3 mouse
PROiPR:Q99JY9
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026341 Expressed in 44 organ(s), highest expression level in spleen
CleanExiMM_ACTR3
ExpressionAtlasiQ99JY9 baseline and differential
GenevisibleiQ99JY9 MM

Family and domain databases

InterProiView protein in InterPro
IPR004000 Actin
IPR020902 Actin/actin-like_CS
IPR015623 Arp3
PANTHERiPTHR11937 PTHR11937, 1 hit
PTHR11937:SF175 PTHR11937:SF175, 1 hit
PfamiView protein in Pfam
PF00022 Actin, 1 hit
SMARTiView protein in SMART
SM00268 ACTIN, 1 hit
PROSITEiView protein in PROSITE
PS01132 ACTINS_ACT_LIKE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiARP3_MOUSE
AccessioniPrimary (citable) accession number: Q99JY9
Secondary accession number(s): Q9DC56
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 150 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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