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Protein

Eukaryotic translation initiation factor 3 subunit M

Gene

Eif3m

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.UniRule annotation

GO - Molecular functioni

GO - Biological processi

  • cytoplasmic translational initiation Source: GO_Central
  • formation of cytoplasmic translation initiation complex Source: UniProtKB-UniRule
  • translation Source: MGI

Keywordsi

Molecular functionInitiation factor
Biological processProtein biosynthesis

Enzyme and pathway databases

ReactomeiR-MMU-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-MMU-72702 Ribosomal scanning and start codon recognition

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 3 subunit MUniRule annotation
Short name:
eIF3mUniRule annotation
Alternative name(s):
PCI domain-containing protein 1
Gene namesi
Name:Eif3m
Synonyms:Pcid1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1351744 Eif3m

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedUniRule annotation
ChainiPRO_00003081962 – 374Eukaryotic translation initiation factor 3 subunit MAdd BLAST373

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineUniRule annotationBy similarity1
Modified residuei2PhosphoserineBy similarity1
Modified residuei152PhosphoserineBy similarity1
Modified residuei254N6-acetyllysineBy similarity1
Modified residuei367PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ99JX4
MaxQBiQ99JX4
PaxDbiQ99JX4
PeptideAtlasiQ99JX4
PRIDEiQ99JX4

PTM databases

iPTMnetiQ99JX4
PhosphoSitePlusiQ99JX4
SwissPalmiQ99JX4

Expressioni

Gene expression databases

BgeeiENSMUSG00000027170 Expressed in 47 organ(s), highest expression level in female gonad
ExpressionAtlasiQ99JX4 baseline and differential
GenevisibleiQ99JX4 MM

Interactioni

Subunit structurei

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3.UniRule annotation

GO - Molecular functioni

Protein-protein interaction databases

BioGridi221017, 3 interactors
DIPiDIP-59784N
IntActiQ99JX4, 3 interactors
MINTiQ99JX4
STRINGi10090.ENSMUSP00000028592

Structurei

3D structure databases

ProteinModelPortaliQ99JX4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini180 – 339PCIPROSITE-ProRule annotationAdd BLAST160

Sequence similaritiesi

Belongs to the eIF-3 subunit M family.UniRule annotation

Phylogenomic databases

eggNOGiKOG2753 Eukaryota
ENOG410XNP7 LUCA
GeneTreeiENSGT00390000004456
HOGENOMiHOG000112351
HOVERGENiHBG107844
InParanoidiQ99JX4
KOiK15030
OMAiVFTGVDQ
OrthoDBiEOG091G0H3D
PhylomeDBiQ99JX4
TreeFamiTF106148

Family and domain databases

HAMAPiMF_03012 eIF3m, 1 hit
InterProiView protein in InterPro
IPR016024 ARM-type_fold
IPR027528 eIF3m
IPR000717 PCI_dom
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF01399 PCI, 1 hit
SMARTiView protein in SMART
SM00088 PINT, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF48371 SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS50250 PCI, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

Q99JX4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSVPAFIDIS EEDQAAELRA YLKSKGAELS EENSEGGLHV DLAQIIEACD
60 70 80 90 100
VCLKEDDKDV ESVMNSVVSL LLILEPDKQE ALIESLCEKL VKFREGERPS
110 120 130 140 150
LRLQLLSNLF HGMDKNTPVR YTVYCSLIKV AASCGAIQYI PTELDQVRKW
160 170 180 190 200
ISDWKLTTEK KHTLLRLLYE ALVDCKKSDA ASKVMVELLG SYTEDNASQA
210 220 230 240 250
RVDAHRCIVR ALKDPNAFLF DHLLTLKPVK FLEGELIHDL LTIFVSAKLA
260 270 280 290 300
SYVKFYQNNK DFIDSLGLLH EQNMAKMRLL TFMGMAVENK EISFDTMQQE
310 320 330 340 350
LQIGADDVEA FVIDAVRTKM VYCKIDQTQR KVVVSHSTHR TFGKQQWQQL
360 370
YDTLNAWKQN LNKVKNSLLS LSDT
Length:374
Mass (Da):42,517
Last modified:June 1, 2001 - v1
Checksum:i09845D87E792B783
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A2A702A2A702_MOUSE
Eukaryotic translation initiation f...
Eif3m RP24-297H17.12-004
242Annotation score:
A2A701A2A701_MOUSE
Eukaryotic translation initiation f...
Eif3m RP24-297H17.12-003
143Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti29L → I in AAH89568 (PubMed:15489334).Curated1
Sequence conflicti141P → Q in BAE40042 (PubMed:16141072).Curated1
Sequence conflicti155K → N in AAH89568 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK077480 mRNA Translation: BAC36821.1
AK084738 mRNA Translation: BAC39270.1
AK146222 mRNA Translation: BAE26990.1
AK168066 mRNA Translation: BAE40042.1
AL606494 Genomic DNA Translation: CAM17236.1
BC005598 mRNA Translation: AAH05598.1
BC089568 mRNA Translation: AAH89568.1
BC103795 mRNA Translation: AAI03796.1
BC116787 mRNA Translation: AAI16788.1
BC116789 mRNA Translation: AAI16790.1
CCDSiCCDS16495.1
RefSeqiNP_663355.1, NM_145380.2
UniGeneiMm.379278

Genome annotation databases

EnsembliENSMUST00000028592; ENSMUSP00000028592; ENSMUSG00000027170
GeneIDi98221
KEGGimmu:98221
UCSCiuc008lkl.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK077480 mRNA Translation: BAC36821.1
AK084738 mRNA Translation: BAC39270.1
AK146222 mRNA Translation: BAE26990.1
AK168066 mRNA Translation: BAE40042.1
AL606494 Genomic DNA Translation: CAM17236.1
BC005598 mRNA Translation: AAH05598.1
BC089568 mRNA Translation: AAH89568.1
BC103795 mRNA Translation: AAI03796.1
BC116787 mRNA Translation: AAI16788.1
BC116789 mRNA Translation: AAI16790.1
CCDSiCCDS16495.1
RefSeqiNP_663355.1, NM_145380.2
UniGeneiMm.379278

3D structure databases

ProteinModelPortaliQ99JX4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi221017, 3 interactors
DIPiDIP-59784N
IntActiQ99JX4, 3 interactors
MINTiQ99JX4
STRINGi10090.ENSMUSP00000028592

PTM databases

iPTMnetiQ99JX4
PhosphoSitePlusiQ99JX4
SwissPalmiQ99JX4

Proteomic databases

EPDiQ99JX4
MaxQBiQ99JX4
PaxDbiQ99JX4
PeptideAtlasiQ99JX4
PRIDEiQ99JX4

Protocols and materials databases

DNASUi98221
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028592; ENSMUSP00000028592; ENSMUSG00000027170
GeneIDi98221
KEGGimmu:98221
UCSCiuc008lkl.1 mouse

Organism-specific databases

CTDi10480
MGIiMGI:1351744 Eif3m

Phylogenomic databases

eggNOGiKOG2753 Eukaryota
ENOG410XNP7 LUCA
GeneTreeiENSGT00390000004456
HOGENOMiHOG000112351
HOVERGENiHBG107844
InParanoidiQ99JX4
KOiK15030
OMAiVFTGVDQ
OrthoDBiEOG091G0H3D
PhylomeDBiQ99JX4
TreeFamiTF106148

Enzyme and pathway databases

ReactomeiR-MMU-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-MMU-72702 Ribosomal scanning and start codon recognition

Miscellaneous databases

PROiPR:Q99JX4
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027170 Expressed in 47 organ(s), highest expression level in female gonad
ExpressionAtlasiQ99JX4 baseline and differential
GenevisibleiQ99JX4 MM

Family and domain databases

HAMAPiMF_03012 eIF3m, 1 hit
InterProiView protein in InterPro
IPR016024 ARM-type_fold
IPR027528 eIF3m
IPR000717 PCI_dom
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF01399 PCI, 1 hit
SMARTiView protein in SMART
SM00088 PINT, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF48371 SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS50250 PCI, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiEIF3M_MOUSE
AccessioniPrimary (citable) accession number: Q99JX4
Secondary accession number(s): Q3TI04, Q5EBI5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: June 1, 2001
Last modified: October 10, 2018
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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